鸡血藤的研究进展

目的对鸡血藤的研究进展加以综述。方法结合国内外最新文献,从化学成分、药理活性等方面对鸡血藤的研究进展进行归纳与整理。结果与结论鸡血藤植物资源丰富,具有独特的药理活性与应用,对其进行深入研究及开发有意义。     

Deficiency of p67phox, p47phox or gp91phox in chronic granulomatous disease does not impair leucocyte chemotaxis or motility

Chronic granulomatous disease (CGD) is a syndrome characterized by failure of the NADPH oxidase of phagocytes that generates superoxide, which is central to the microbicidal process. Cytosolic components of this oxidase system include the proteins p67 ^phox and p47 ^phox , deficiencies of which cause the autosomal recessive form of CGD, whereas the X-linked form of the disease is characterized by a deficiency in the plasma membrane component gp91 ^phox . Components of the oxidase system have been reported to be associated with the cytoskeleton and neutrophils from CGD patients have been reported to have a defective chemotactic response in Boyden chambers. Using a chamber that permits the direct observation of cell behaviour in a linear gradient of a chemoattractant, we have analysed the chemotactic response of neutrophils from a patient lacking p67 ^phox ; from another lacking p47 ^phox and from a third lacking gp91 ^phox . The results of measuring the speeds and directions of locomotion of the cells show that their speeds are undiminished relative to cells from healthy control subjects and that their directions of migration are at least as strongly biased in the direction of the gradient as those of the control cells. We conclude that these definitive aspects of the chemotactic response are not abnormal in either the autosomal recessive or the X-linked forms of CGD and that they are therefore not factors in the predisposition to infection that characterizes the syndrome.     

鸡血藤化学成分的分离与结构鉴定

目的对中药鸡血藤(Spatholobus suberectus Dunn)中化学成分进行分离及结构鉴定。方法采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱及高效液相色谱等手段进行分离纯化,并通过理化性质与波谱分析方法鉴定了化合物的结构。结果从鸡血藤体积分数为95%的乙醇提取物中分离鉴定了9个单体成分,分别为blumenol A(1)、(6S,7E,9R)-roseoside(2)、(6S,7E,9R)-6,9-dihydroxy-4,7-megastigman-3-one-9-O-[α-L-arabinopyranosyl-(1→6)-(β-D-glucopyranoside](3)、7S,8R-erythro-4,9,9'-trihydroxy-3,3'-dimethoxy-8-O-4'-neolignan-7-O-β-D-glucopyranoside(4)、(7S,8R)-dihydrodehydrodiconiferyl alcohol-4-O-(β-D-glucopyranoside(5)、(7S,8R)-3,3',5-trimethoxy-4',7-epoxy-8,5'-neolignan-4,9,9'-triol(6)、次黄苷(hypoxanthine-9-β-D-ribofuranoside,7)、烟酸(nicotinic acid,8)和丁二酸(amber acid,9)。结论其中2-8均为首次从密花豆属中分离得到的化合物。     

HPLC-TOF/MS对中药猫人参化学成分的快速鉴别

目的运用高效液相-高分辨飞行时间质谱(HPLC-TOF/MS)技术快速鉴别中药猫人参化学成分。方法色谱分离采用SHISEIDO MG C18(3.0 mm×100 mm,3μm)色谱柱;流动相为乙腈和0.1%甲酸水溶液,梯度洗脱,0¹0 min:10%10 min:10%²5%A,1025%A,10³5 min:25%35 min:25%⁹0%A,3590%A,35⁴0 min:90%A;柱温25℃;流速0.6 mL·min-1,柱后分流比为2:1。质谱定性采用飞行时间质谱,电喷雾离子源(ESI),正离子模式,质量数扫描范围m/z 10040 min:90%A;柱温25℃;流速0.6 mL·min-1,柱后分流比为2:1。质谱定性采用飞行时间质谱,电喷雾离子源(ESI),正离子模式,质量数扫描范围m/z 100¹ 000。结果一次性鉴别出猫人参中15种的化学成分,其中2种成分首次从猫人参中发现。结论建立了一种基于HPLC-TOF/MS技术对猫人参中化学成分进行快速鉴别的方法,为中药猫人参的质量控制及体内的深入研究奠定了基础。     

Differentiation of Dunn osteosarcoma cells in response to dibutyryl cyclic 3′,5′-adenosine monophosphate

We investigated the effects of dibutyryl cyclic adenosine 3,5-monophosphate (Bt₂cAMP) on the differentiation of Dunn osteosarcoma cells. Flow-cytometric analysis and DNA synthesis assay showed that Bt₂cAMP decreased the cell population in the S phase in a time- and dose-dependent manner. Also, the cells showed distinct morphological and functional alterations: the cell morphology changed to a fibroblast-like appearance with long and thin protoplasmic processes, the knobs or blebs on both the cell membrane and nuclear membrane disappeared and the intracellular alkaline phosphatase activity increased. Moreover, Bt₂cAMP-treated cells secreted a large quantity of fibronectin, which was deposited on the extended cell surface in the culture medium. Thus, Dunn osteosarcoma cells are differentiated morphologically and functionally by Bt₂cAMP, and might be transformed to benign precursor cells.Abbreviation Bt₂cAMP dibutyryl cyclic 3,5-adenosine monophosphate This research was supported by a grant-in-aid for eucouragement of young scientists from the Japanese Ministry of Education, Science and Culture (02770900)     

龙眼参多糖对不同模型拟痴呆小鼠的益智作用

目的　探讨龙眼参多糖对记忆障碍小鼠学习记忆功能的影响。方法　从龙眼参提取多糖 ,以东莨菪碱、40 0ml/L乙醇和D -半乳糖复制记忆障碍小鼠模型 ,以水迷宫为学习记忆评价指标 ,观察不同剂量的多糖对小鼠学习记忆的影响。结果　在东莨菪碱、40 0ml/L乙醇和D -半乳糖复制记忆障碍小鼠模型中 ,龙眼参多糖组与模型组比较 ,水迷宫的正确次数差异均有显著性 (P <0 .0 5 )。结论　龙眼参多糖能明显增强小鼠的学习记忆功能。     

HPLC法测定玉郎伞中水黄皮素的含量

[目的]建立高效液相色谱法测定玉郎伞中水黄皮素含量的方法。[方法]以CLC-ODS（6.0 mm×150 mm,5μm）为色谱柱,[0.05 mol/L庚烷磺酸钠–0.05 mol/L磷酸二氢钾（3∶5）]–乙腈（1∶1）用磷酸调pH 3.0为流动相,流速1.0 ml/min,检测波长261 nm,柱温30℃。[结果]水黄皮素的线性范围为0.099～0.998μg,平均回收率为100.3%（RSD=1.2%）。[结论]所建立的HPLC法可用于玉郎伞中水黄皮素含量测定及质量控制。     

RP-HPLC法测定鸡血藤中芒柄花素的含量

目的建立测定鸡血藤药材中芒柄花素的RP-HPLC法。方法采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(体积比为35∶65),流速为1 mL.min-1,检测波长为254 nm,柱温为30℃。结果芒柄花素在0.5~15.4 mg.L-1内线性关系良好(r=0.999 8,n=6),平均回收率为98.6%(RSD=1.6%,n=9)。结论RP-HPLC法可用于鸡血藤中芒柄花素的含量测定。     

密花豆扦插苗与种子苗的生长及光合特性研究

目的 探究密花豆(Spatholobus suberectus Dunn)扦插苗与种子苗的生长动态和光合特性差异.方法 通过盆栽及大田栽培试验,对同龄密花豆扦插苗与种子苗的株高、基径、成活率及光合特性进行对比分析.结果 在盆栽及大田试验中,密花豆扦插苗与种子苗的株高和基径均呈直线增大趋势,且种子苗的长势优于扦插苗.盆栽...     

狭叶香茶菜二萜成分的研究

A spirosecokaurenoid, angustifolin (Ⅰ) was isolated from the leaves of Rabdosia angustifolia (Dunn) Hara (Labiatae) and its structure was established from spectral and chemical evidence. One known diterpenoid, isodonal (V) was also isolated together with β-sitosterol. (Ⅰ) and (Ⅴ) showed in vitro cytotoxicity and in vivo tumor inhibitory activities.