小儿脓毒症CD62p、CD63及CD64分子变化及临床意义探讨

目的探讨脓毒症患儿血小板仅颗粒膜糖蛋白（CD62p）、溶酶体膜蛋白（CD63）及中性粒细胞表面膜糖蛋白（CD64）的变化及意义。方法2010年3月至2013年3月哈尔滨市儿童医院感染内科收治的脓毒症患儿56例,其中严重脓毒症16例,一般脓毒症40例,另设健康对照组34例。应用流式细胞术检测儿童外周血的血小板表面活性标记糖蛋白CD62p、CD63及中性粒细胞CD64表达,并进行比较。结果严重脓毒症组患儿外周血的CD62p、CD63及CD64水平明显高于一般脓毒症组,差异有统计学意义（P〈0．01）;一般脓毒症组患儿CD62p、CD63及CD64水平明显高于健康对照组,差异有统计学意义（P〈0．01）。相关性分析显示,脓毒症患儿的CD62p、CD63与CD64水平呈正相关（r=0．817、0．796,P均〈0．001）,CD62p、CD63、CD64水平与小儿危重病例评分呈正相关（CD62p：r=0．883,P〈0．001;CD63：r=0．862,P〈0．001;CD64：r=0．805,P〈0．001）。结论CD62p、CD63、CD64与感染和炎症严重程度密切相关,可作为评价疾病严重程度及预后的指标。     

64排螺旋CT对肺孤立性结节的征象分析及诊断价值

目的研究64排螺旋CT孤立性肺结节（SPN）影像学特征,提高良恶性结节的诊断准确性。 方法收集连云港市第二人民医院2015年8月至2018年12月病理确诊的170例SPN,恶性结节（116例）作为肺腺癌组,良性结节（54例）作为良性结节组,采用χ²检验比较组间结节的CT表现（位置、病灶形态、内部成分、瘤-肺界面、瘤周毛刺征、胸膜牵拉征、支气管截断征等）及强化方式的差异,采用t检验比较组间病灶直径和距胸膜距离的差异。 结果肺腺癌组与良性结节组比较,实性、亚实性结节（42.2% vs 22.2%、44.8% vs 29.6%）、不规则形（88.7% vs 35.2%）、瘤-肺界面不光滑（77.6% vs 22.2%）、分叶（62.9% vs 14.8%）、瘤周毛刺征（56.9% vs 3.7%）、胸膜牵拉征（45.7% vs 3.7%）等征象表现所占比例均大,且差异均具有统计学意义（χ²=25.17、52.24、47.03、23.54、43.43、29.66,P均＜0.05）;2组间病灶直径和距胸膜距离比较,差异无统计学意义（P＞0.05）。增强动脉期肺腺癌组结节轻-中度度强化比例高于良性结节组（57.4% vs 28.6%）,结节明显强化比例低于良性结节组（35.2% vs 53.5%）,差异具有统计学意义（χ²=4.68,P＜0.05）;静脉期肺腺癌组结节延迟强化比例高于良性结节组（83.3% vs 53.5%）,结节强化减退比例低于良性结节组（9.2% vs 46.5%）,差异具有统计学意义（χ²=64.23,P＜0.05）。 结论CT是检出肺结节的主要方法,通过特征性表现结合增强特点可以提高影像诊断准确性,尽早对肺癌结节进行正确的临床干预。     

评价64排螺旋CT三维重建技术评估稳定性骨盆骨折的应用价值

目的:评价64排螺旋CT三维重建技术评估稳定性骨盆骨折的应用价值。方法:回顾性分析我院2015年6月-2019年6月收治的20例骨盆骨折患者的临床资料,结合手术结果,对64排螺旋CT三维重建及X线两种影像学检查方式对各个部位骨折诊断准确率进行观察和比较。结果:在不同部位骨折检查中,64排螺旋CT三维重建诊断准确率皆比X线检查高(P<0.05),且骨折总诊断准确率明显比X线检查高(P<0.05)。结论:在骨盆骨折诊断方式中,CT检查有效率较高,而对64排螺旋CT三维重建技术加以应用,可使盆骨骨折诊断准确率得到进一步提高。     

A single amino acid substitution modulates low-pH-triggered membrane fusion of GP64 protein in Autographa californica and Bombyx mori nucleopolyhedroviruses

We have previously shown that budded viruses of Bombyx mori nucleopolyhedrovirus (BmNPV) enter the cell cytoplasm but do not migrate into the nuclei of non-permissive Sf9 cells that support a high titer of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) multiplication. Here we show, using the syncytium formation assay, that low-pH-triggered membrane fusion of BmNPV GP64 protein (Bm-GP64) is significantly lower than that of AcMNPV GP64 protein (Ac-GP64). Mutational analyses of GP64 proteins revealed that a single amino acid substitution between Ac-GP64 H155 and Bm-GP64 Y153 can have significant positive or negative effects on membrane fusion activity. Studies using bacmid-based GP64 recombinant AcMNPV harboring point-mutated ac-gp64 and bm-gp64 genes showed that Ac-GP64 H155Y and Bm-GP64 Y153H substitutions decreased and increased, respectively, the multiplication and cell-to-cell spread of progeny viruses. These results indicate that Ac-GP64 H155 facilitates the low-pH-triggered membrane fusion reaction between virus envelopes and endosomal membranes.     

64层螺旋CT冠状动脉成像对冠状动脉疾病的诊断价值

目的评价64层螺旋CT冠状动脉成像（64SCTCA）在诊断冠状动脉疾病中的临床价值。方法收集60名患者64SCTCA的完整资料,与近期冠状动脉造影术结果进行对照分析。结果CAG发现92支血管有狭窄,64SCTCA发现98支血管有狭窄,冠状动脉CTA诊断狭窄的敏感性为94.6%,特异性95.9%。结论64SCTCA对于冠状动脉狭窄的评估具有一定敏感性和特异性,用于冠心病的临床筛查具有重要意义。     

Two-generation reproductive toxicity evaluation of dietary 17beta-estradiol (E2; CAS No. 50-28-2) in CD-1 (Swiss) mice.

No information exists on reproductive/developmental effects in mice exposed to dietary 17beta-estradiol (E2) over multiple generations. Therefore, under OECD Test Guideline 416 with enhancements, CD-1 mice (F0 generation, 25 mice/sex/group) were exposed to dietary E2 at 0, 0.001, 0.005, 0.05, 0.15, or 0.5 ppm ( approximately 0, 0.2, 1, 10, 30, or 100 mug E2/kg body weight/day) for 8 weeks prebreed, 2 weeks mating, approximately 3 weeks gestation, and 3 weeks lactation. At weaning, selected F1 offspring (F1 parents; 25/sex/group) and extra retained F1 males (one per litter) were exposed to the same dietary concentrations and durations as the F0 generation; study termination occurred at F2 weaning; F1/F2 weanlings (up to three per sex per litter) were necropsied with organs weighed. At 0.5 ppm, effects were increased F1/F2 perinatal loss, prolonged F0/F1 gestational length, reduced numbers of F2 (but not F1) litters/group, reduced F1/F2 litter sizes, accelerated vaginal patency (VP) and delayed preputial separation (PPS), increased uterus + cervix + vagina weights (UCVW) in F0/F1 adults and F1/F2 weanlings, and decreased testes and epididymides weights (TEW) in F1/F2 weanlings. At 0.15 ppm, effects were increased UCVW in F0/F1 adults and F1/F2 weanlings, accelerated VP, delayed PPS, and reduced TEW in F1/F2 weanlings. At 0.05 ppm, UCVW were increased in F1/F2 weanlings, and PPS was delayed only in extra retained F1 males. There were no biologically significant or treatment-related effects on F0/F1 parental body weights, feed consumption, or clinical observations, or on F0/F1 estrous cyclicity, F0/F1 andrology, or F1/F2 anogenital distance at any dose. The no observable effect level was 0.005 ppm E2 ( approximately 1 mug/kg/day). Therefore, the mouse model is sensitive to E2 by oral administration, with effects on reproductive development at doses of 10- 100 mug/kg/day.     

双源64层CT的工作原理及其临床应用

本文对双源64层CT的基本结构、工作原理及其临床应用做了介绍。     

Ulrich注射器在64排螺旋CTA检查中的应用价值及护理

目的:评价Ulrich造影剂注射器在64排螺旋CTA检查中的应用价值。方法:使用GE-Lightspeed VCT64排螺旋CT机,德国Ulrich Missouvi(XD2001)造影剂注射器,对比剂碘帕醇370mg/mg,注射总量80～100mL。所有病人留置静脉套管针(20G～22G)。注射方案采用:注入生理盐水20ml4～5ml/s,确保血管对高压注射器的承受力。结果:我院2007年4月至2008年1月进行CTA检查105例病人无1例渗漏,对比剂顺利注入,造影剂注射成功率达到100%,无1例发生严重过敏反应。结论:使用Ulrich造影剂注射器,操作简便,注射成功率高,能提高工作效率,减少病人候诊时间。其功能完全可以适应64排螺旋CT检查的要求。     

64排多层螺旋CT与冠脉造影在冠心病介入诊疗应用中的对比研究

目的:评价64排多层螺旋CT在冠心病诊断中的临床应用价值。方法:选择2005年8月-2006年5月共21例经过冠脉造影病人的67支血管分为185个节段,以冠脉造影结果为金标准,将其CT影像与冠脉造影结果进行对比分析。结果:三维重建64排CT血管造影(CTA)诊断冠状动脉病变总的敏感性(Sn)95.5%,特异性(Sp)96.6%,准确度(Ac)96.2%,阳性预测值( PV)94.1%,阴性预测值(-PV)97.4%。结论:64排多层螺旋CT做为无创的筛查冠心病的手段,其结果可靠,应予推广。     

A selective plasmin inhibitor, trans-aminomethylcyclohexanecarbonyl-L-(O-picolyl)tyrosine-octylamide (YO-2), induces thymocyte apoptosis

The treatment of rat thymocytes with YO-2, a novel inhibitor of plasmin, resulted in an increase in DNA fragmentation. DNA fragmentation was also induced by another YO compounds such as YO-0, -3, -4 and -5. These YO compounds are the inhibitor of plasmin activity. On the other hand, YO-1, -6 and -8 that hardly inhibit plasmin activity had no effect on DNA fragmentation. Analysis of fragmented DNA from thymocytes treated with YO-2 by agarose gel electrophoresis revealed that the compound caused internucleosomal DNA fragmentation. In addition, judging from a laser scanning microscopy, annexin V-positive and propidium iodide-negative cells were increased by the treatment of the cells with the compound. Moreover, chromatin condensation was observed in thymocytes treated with the compound. These results demonstrated that YO-2 induces thymocyte apoptosis. There seemed to be some correlation between the apoptosis induced by YO compounds and their plasmin inhibitory effect. However, because the other protease inhibitors including pepstatin A, leupeptin, AEBSF, DFP and E-64-d did not affect DNA fragmentation, YO compounds are likely to have unique mechanism on plasmin or to show the effect on the other plasmin-like proteases. The plasmin inhibitory activity may have an important role in YO-2-induced apoptosis. Furthermore, the stimulations of caspase-8, -9 and -3-like activities were observed in thymocytes treated with YO-2. These results suggest that YO-2 induces thymocyte apoptosis via activation of caspase cascade.