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61.
为给临床心脏术后监测心淋巴管损伤程度及可能出现的并发症提供参考指标,以手术方法阻断羊心淋巴流并对其血清相关成份进行了测定。实验发现,实验组术后谷草转氨酶(SGOT)、谷丙转氨酶(SGPT)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)均有不同程度的增高,以SGOT增高最为明显,术前均值为47.53U,术后2天增至104.20U,高峰出现时间最早,多在术后24小时。血清离子改变中低钾最为突出,血钾在术后2天明显降低(P<0.01),术后7天逐渐恢复,术后14天恢复至术前水平。血清蛋白改变以球蛋白降低、白蛋白/球蛋白比值升高为主。提示心淋巴循环郁滞可导致心肌细胞损伤、毛细血管通透性和结构的改变。不同酶活性的变化对郁滞的敏感度存在着差异。 相似文献
62.
本文用胶固素酶联免疫吸附试验检测了16例缺血性脑血管病(ICD)患者,14例出血性脑血管病(HCD)患者和20例健康正常人的血清IgE型循环免疫复合物(CID)水平。结果显示ICD组和HCD组IgE-CIC水平明显高于正常组(分别为P<0.001和P<0.05)。ICD组与HCD组间没有显著差异(P>0.02)。提示脑血管病的发生与IgE-CIC的形成有关。 相似文献
63.
对沈阳市某幼儿园99例3~6岁锌缺乏儿童的营养调查和生物样品(血液、尿液和毛发)的分析发现:同对照组儿童相比,锌缺乏儿童除Fe、维生素A外,其他主要营养素的摄入明显不足;缺锌儿童的发锌、RBC锌水平、血清ALP活性、尿锌/尿肌酐比值均明显低于对照组。经补锌治疗后血浆锌水平、血清ALP活性、尿锌/尿肌酐比值显著升高。应用临床诊断实验的评价方法分析发现结合血浆锌水平,联合使用发锌,血清ALP和尿锌/尿肌酐比值3项指标可以准确判断儿童边缘性锌缺乏,这时并联的灵敏度为96.18%,串联的特异度可高达到100%(血清ALP活性<104IU/L,同时发锌含量<110ppm.尿锌/尿肌酐比值<0.3μg/g)。 相似文献
64.
L. Lund H. Henmar P. A. Würtzen G. Lund N. Hjortskov J. N. Larsen 《Clinical and experimental allergy》2007,37(4):564-571
BACKGROUND: Specific immunotherapy with intact allergen vaccine is a well-documented treatment for allergic diseases. Different vaccine formulations are currently commercially available, the active ingredient either being intact allergens or chemically modified allergoids. The rationale behind allergoids is to decrease allergenicity while maintaining immunogenicity. However, data from the German health authorities based on reporting of adverse events over a 10-year period did not indicate increased safety of allergoids over intact allergens. OBJECTIVE: The objective of this study was to investigate the effect of chemical modification on allergenicity and immunogenicity comparing four commercial allergoid products for birch pollen immunotherapy with an intact allergen vaccine. METHODS: Solid-phase IgE inhibition and histamine release assays were selected as model systems for allergenicity, and a combination of human T cell proliferation and IgG titres following mouse immunizations were used to address the immunogenicity of the intact allergen vaccine and the four allergoids. In all assays, the products were normalized with respect to the manufacturer's recommended maintenance dose. RESULTS: IgE inhibition experiments showed a change in epitope composition comparing intact allergen vaccine with allergoid. One allergoid product induced enhanced histamine release compared to the intact allergens, while the other three allergoids showed reduced release. Standard T cell stimulation assays using lines from allergic patients showed a reduced response for all allergoids compared with the intact allergen vaccine regardless of the cell type used for antigen presentation. All allergoids showed reduced capacity to induce allergen-specific IgG responses in mice. CONCLUSION: While some allergoids were associated with reduced allergenicity, a clear reduction in immunogenicity was observed for all allergoid products compared with the intact allergen vaccine, and the commercial allergoids tested therefore do not fulfil the allergoid concept. 相似文献
65.
Crossed radioimmunoelectrophoresis (CRIE) was used to study the presence of serum IgE against antigenic components of cow milk in 21 selected milk-allergic patients. The amount of each IgE specificity was estimated by a scoring system. The milk-allergic children had mainly IgE against alpha-lactalbumin, beta-lactoglobulin, albumin and immunoglobulin, the four major proteins of bovine whey, as well as IgE against three casein components. A serum pool from 1000 normal adults had IgE against the same whey protein, but in smaller amounts, and no IgE against the casein components. Eight cow milk-based formulae, commonly used for infant feeding, and goat milk were studied by the same method. It was found that six of the milk substitutes did not differ significantly from cow milk in antibody binding, but the two hydrolysed casein products, Nutramigen and Pregestimil, consisted of such small molecules that the rabbit antisera could not precipitate the hydrolysed proteins in the gels on the CRIE plates. It was therefore not possible to study their IgE binding, if any, by this method. 相似文献
66.
Twenty-six patients diagnosed as chronic schizophrenics were given injections of zuclopenthixol decanoate (cis(Z)-clopenthixol decanoate) 200 mg every 3 weeks for at least 6 months. Before treatment and on each day of injection the
patients' mental state was assessed by Brief Psychiatric Rating Scale (BPRS), 18 items. A registration of side effects and
basal laboratory data was also performed. Blood samples were drawn on each day of injection before injection and 3–7 days
after injection (time of maximum concentration). Neuroleptic activity, which was considered equivalent to the concentration
of zuclopenthixol, was determined in serum by radio-receptor assay (RRA). Based on amelioration scores ≥50% on the BPRS, 15
patients were characterized as responders and 11 as non-responders. The responder group showed a statistically significant
reduction in BPRS score, whereas this was not the case for the non-responders. Apart from a few patients, the serum concentrations
showed a low intra-individual variation, but a relatively high inter-individual variation. The responder group had a significantly
higher mean preinjection concentration than the non-responder group, whereas no significant difference was found in day 3–7
concentrations. The fluctuation of the serum concentration expressed as the ratio between maximum (days 3–7) and minimum (pre-inj.)
was found to be significantly lower for responders than for non-responders. Thus although the present study did not demonstrate
a clear relationship between serum level and clinical effect, it indicates that the best antipsychotic effect is obtained
with a serum concentration which fluctuates only slightly (the ratio max/min concentration not exceeding 2.1). 相似文献
67.
腺病毒载体介导CTLA4Ig基因治疗小鼠变应性鼻炎 总被引:1,自引:0,他引:1
目的 检测CTLA4Ig-重组腺病毒载体(Ad-CTLA4Ig)在变应性鼻炎治疗中的作用。方法 采用卵清蛋白(OVA)致敏和激发诱导小鼠变应性鼻炎。实验组在OVA激发前30min予以Ad-CTA4Ig腹腔注射。未转CTLA4IgcDNA的腺病毒载体(Adv)作对照。比较各组动物鼻部症状和鼻粘膜形态学改变,并采用ELISA法测定血清中OVA-特异性IgE水平。结果 CTLA4Ig重组腺病毒实验组小鼠鼻部症状和鼻粘膜病理改变不明显,并且血清中OVA-特异性IgE水平明显低于对照组(P<0.05)。结论 Ad-CTLA4Ig可防治小鼠应变性鼻炎的发生,提示CTLA4Ig-重组腺病毒载体有可能运用于临床变应性鼻炎的治疗。 相似文献
68.
Objective: To evaluate the diagnostic value of serum neuron specific enolase and Video EEG inFebrile Convulsion of childen. Method:Serum NSE was detected by RIA on the first day and the seventh day afterseizure in 40 children with simple febrile convulsion and 18 with complex febrile convulsion. Video EEG was per-formed at 1st, 7th and 30th day in all the patients. Results: There were significant differences between NSE levels at24th hour and on 7th day after convulsion (P<0.01). NSE concentrations in patients with SFC and CFC were also 相似文献
69.
Summary A rapid and quantitative method for the determination of benzodiazepines using high-performance liquid chromatography (HPLC)
with diode-array detection (DAD) is reported. The drugs were extracted from serum, blood or post-mortem blood using C18 extraction columns. Brotizolam was used as internal standard. Experiments with spiked serum/blood samples resulted in recoveries
between 75% and 94% for all investigated benzodiazepines. Excellent linearity was obtained over the concentration range 5–1500
ng benzodiazepine/ml. The limit of detection was approximately 2 ng/ml. The detection of low therapeutic serum levels of highly
potent benzodiazepines is also possible.
相似文献
70.
目的探讨早产儿血清表皮生长因子水平与脑发育的关系。方法依据入选标准观察对象为自2005年8月起连续收治入本院新生儿病房、出生24h内的胎龄为34周±3d的“正常”早产适于胎龄儿30例。采用酶联免疫吸附测定(ELISA)法,分别测定30例早产儿生后24h内和出生第8天空腹股静脉血清表皮生长因子(EGF)水平,分别为t0和t1值,计算血清EGF浓度变化的速率(Δt)。同时,在新生儿出生后6周(纠正胎龄接近40周)和纠正1月龄时进行NBNA评分测定。并将以上实验室数据与新生儿行为神经测定(NBNA)评分值及差值、早产儿出生后的头围增长值及增长速率进行Pearson相关分析。如果P值<0.05,则认为差有统计学意义。结果30例早产儿观察对象t0为(234.71±58.07)pg/ml,t1为(347.81±87.49)pg/ml,Δt为(16.16±9.94)(12.14、15.20)pg/ml/d;Pearson相关分析显示2次NBNA评分结果及差值、出生后6周和纠正1月龄时的头围增长值/增长速率与t0值无相关性(P>0.05);2次NBNA评分结果及差值、出生后6周和纠正1月龄时的头围增长值/增长速率与t1和Δt之间有相关性(P<0.05)。结论研究结果表明,早产儿出生后血清EGF浓度呈上升趋势,提示早产儿出生后体内EGF合成和释放增加,可能促进脏器成熟和组织修复;更重要的是,早产儿出生1周时高水平血清EGF浓度和/或较快上升速率者,其出生后头围增长速度也快,可能有较好的脑发育水平、远期预后良好。 相似文献