义齿用Ti-6Al-7Nb合金铸件挠度的研究

目的：通过对相同长度、宽度、不同厚度的Ti-6Al-7Nb合金铸件挠度的测量,观察铸件厚度与挠度的关系,为确定Ti-6Al-7Nb合金铸造义齿基托的适合厚度提供实验依据。方法：参照ISO关于义齿基托材料挠度的标准,测量不同厚度Ti-6Al-7Nb合金铸件的挠度,并与纯钛、Ti-6Al-4V合金及Co-Cr合金对比。结果：Ti-6Al-7Nb合金、纯钛及Ti-6Al-4V合金铸件的厚度在0.65mm时可以达到义齿基托挠度的标准;Co-Cr合金在0.57mm时可达到标准要求。结论：在临床制作Ti-6Al-7Nb合金义齿基托时,厚度应不低于0.65mm。     

大肠癌组织中细胞胀亡及MMP-2检测

目的 :检测大肠癌组织中胀亡细胞及MMP - 2。方法 :应用透射电镜和免疫组化方法检测 5 1例大肠癌组织中胀亡细胞和MMP - 2的表达。结果 :①大肠癌组织中可检测到不同时期的胀亡细胞。②大肠癌组织中MMP- 2表达率 5 4 .9% (2 8/ 5 1 ) ,明显高于正常大肠粘膜 (P <0 .0 5 )。③MMP - 2在淋巴结转移组的阳性表达率为 6 9.5 7% (1 6 / 2 3) ,明显高于无淋巴结转移组的 4 2 .86 % (1 2 / 2 8) ;有淋巴结转移组胀亡指数明显低于无淋巴结转移组 (P<0 .0 5 )。④MMP - 2表达阳性组OI高于MMP - 2阴性组。结论 :大肠癌组织中存在胀亡细胞和MMP - 2的表达 ,2者关系密切 ,且均与大肠癌的转移有关。     

7β-(6-取代-2-喹诺酮-3-乙酰氨基)头孢菌素的合成

以6-取代-2-喹诺酮-3-乙酸为侧链,用CDI法和潘化酯法与7-ADCA,7-ACA,7-ACT,和7-ACD缩合,合成了16个新的7β-(6-取代-2-喹诺酮-3-乙酰氨基)头孢菌素类化合物,通过溶媒转提,葡聚糖凝胶(Sephadex LH-20)柱层析及离心薄层层析分离精制,得到纯品。初步体外抑菌试验表明:新化合物对革兰氏阳性及某些阴性菌具有高度敏感性。大多数化合物对所试试验菌的抗菌活性与头孢唑啉和青霉素G钠相当,有些比它们还强。     

乳腺癌组织中基质金属蛋白酶MMP-9和CD44v6表达及其意义

目的探讨基质金属蛋白酶MMP-9和CD44v6在乳腺癌中的表达及其与乳腺癌侵袭、转移的关系.方法采用免疫组化方法对46例乳腺癌(10例导管内癌,36例乳腺浸润癌),10例乳腺增生组织MMP-9、CD44v6进行标记和分析.结果MMP-9和CD44v6在乳腺增生组织中几乎不表达,导管内癌、乳腺浸润癌表达率显著增高,各组间差异有显著性(P＜0.05),而且MMP-9与CD44v6过表达与淋巴结转移有关.结论MMP-9和CD44v6的过表达在乳腺癌侵袭和转移中发挥重要作用,可作为乳腺癌侵袭和转移的重要分子学标志.     

宫颈癌组织中HPV16 E7序列多态性分析

目的探讨广东地区宫颈癌组织中HPV16肿瘤相关性抗原E7基因序列的多态性.方法采用通用引物PCR直接测序法对宫颈癌标本中的HPV分型,从含有HPV16型的标本中采用自行设计的多重引物通过巢式PCR扩增出HPV16E7,经DNA序列测定法检测其基因变异,进而寻找其热点突变.结果50例宫颈癌组织HPV-DNA的检出率为78%,其中HPV16和HPV18型混合感染18例,单纯HPV16型感染15例.33例含有HPV16型的标本中扩增出25例HPV16E7,其中17例647位核苷酸“T”变异“C”,导致相应的蛋白质由天冬氨酸变异为丝氨酸.结论广东地区宫颈癌组织中HPV16E7DNA序列发生碱基替换的区域主要在647位至846位,热点突变点为Nt647和Nt846.     

Serum markers for fibrosis and plasma transforming growth factor-β1 in patients with hepatocellular carcinoma in comparison with patients with liver cirrhosis

In order to elucidate collagen metabolism in hepatocellular carcinoma (HCC) tissue, we compared levels of different potential markers of collagen metabolism and plasma transforming growth factor-β₁ in patients with HCC and in patients with liver cirrhosis. Serum levels of prolyl hydroxylase and the tissue inhibitor of metalloproteinase-1 in patients with HCC were significantly higher than those in patients with liver cirrhosis and increased with the size of the HCC tumour, whereas the serum levels of procollagen type III propeptide and type IV collagen 7S domain were similar in the two groups. In HCC, the increased plasma transforming growth factor-β₁ levels were closely correlated with serum levels of prolyl hydroxylase and the tissue inhibitor of metalloproteinase-1. These findings suggest that, in HCC tissue, the intracellular biosynthesis of collagen is enhanced, whereas the secretion of procollagen is disturbed and the degradation of collagen is suppressed by the excess production of the tissue inhibitor of metalloproteinase-1. The results also suggest that plasma transforming growth factor-β₁ plays an important role in the altered metabolism of collagen in HCC.     

利用T7和PR双启动子的新型表达载体的构建及其应用

构建具有λＰＲ与Ｔ７双启动动子的新型原核表达载体ｐＤＯＧ，以便使用同一载体研究不同诱导条件下，重组蛋白在大肠杆菌中表达，降解以及折叠的情况，从而选择最佳的诱导条件。方法采用ｐＢＶ２２０与ＰＥＴ－３表达载体作为原始材料，将ＰＥＴ－３上包括Ｔ７启动子与Ｔ７ｇ－１０释译起始序列的一段序列克隆到     

不同来源HSP70的氨基酸组成分析

使用ＤＥＡＥ－５２离子交换层析和ＡＴＰ琼脂糖亲和层析方法，从人体肿瘤细胞株，大鼠肝脏和心脏、家兔和小鼠肝脏组织中纯化了主要的热应激蛋白－ＨＳＰ７０，用氨基酸自动分析仪分析它的氨基酸组成。     

Sequestration patterns of transfused rat neutrophilic granulocytes under normal and inflammatory conditions

Abstract: The fate of polymorphonuclear neutrophilic granulocytes (PMN) after their mobilization from the bone marrow of healthy individuals is not clearly understood. It has been suggested that there is a continuous utilization of these cells in widespread, subclinical inflammatory foci, where they are ultimately degraded. The goal of the present experiments was to determine whether an alternative ecotaxis (“homing”) exists, namely sequestration and degradation of PMN by mononuclear phagocytes exposed to the bloodstream in the liver, spleen and bone marrow. Blood PMN were collected from donor rats, labelled with ⁵¹Cr, and injected i.v. into 2 syngeneic rats, one of them having an induced sterile peritonitis. After various time intervals up to 18 h, the rats were killed and exsanguinated. As expected, we found cell-bound radioactivity in the inflamed peritoneal cavities, and also a high amount of radioactivity in liver, spleen, and bone marrow. The bone marrow uptake of PMN appeared to be much lower in the inflammation rats than in the normal controls. These findings were confirmed in PMN transfer experiments using PVG rats congenic for the RT7 alloantigenic system. Here, transfused blood leukocytes were traced with fluorescent, monoclonal HIS41 antibodies and flow cytometry. A possible corticosteroid effect on the bone marrow sequestration could not be substantiated. Uptake and degradation of PMN takes place in organs containing phagocytes exposed to the bloodstream. Sequestration of PMN in the bone marrow is apparently down-regulated in inflammatory states, perhaps increasing the PMN availability to inflamed tissue.     

MMP-2、TIMP-2在脑胶质瘤中的表达及其意义

目的 分析MMP-2、TIMP-2的表达与脑胶质瘤恶性程度和侵袭性之间的关系。方法 采用免疫组化和原位杂交的方法,检测42例人脑胶质瘤和8例正常脑组织中,MMP-2 mRNA、MMP-2和TIMP-2蛋白表达。结果 MMP-2蛋白表达水平(LI)与肿瘤的分级呈正相关(r=0.872,P<0.01);Ⅲ、Ⅳ级与正常脑组织、Ⅰ～Ⅱ级之间均有显著的差异(P<0.01)。MMP-2mRNA表达与肿瘤的分级同样呈正相关(P<0.01);Ⅲ、Ⅳ级与正常脑组织、Ⅰ～Ⅱ级之间均有显著的差异(P<0.01)。MMP-2蛋白表达增高时TIMP-2蛋白表达也增高;但随着肿瘤恶性程度的增加,TIMP-2表达强度增加的幅度较MMP-2明显减缓。结论 MMP-2的高表达与脑胶质瘤恶性程度呈正相关;TIMP-2与MMP-2间的平衡失调与脑胶质瘤的侵袭性和恶性程度密切相关。