过氧戊二酸对大肠杆菌噬菌体f2杀灭机理

观察结果表明过氧戊二酸对大肠杆菌噬菌休ｆ２有较强的杀灭作用，并影响其吸附特性和感染能力。电镜观察表明过氧戊二酸处理后的ｆ２颗粒聚集成团、变形破碎或残缺不全，这可能是ｆ２灭活的主要原因。     

肺螨类生境研究

本文叙述了肺螨类的孳生环境及其不同生境里肺螨的种群分布,并对分离出的28种螨的生境和肺螨病患者的工作环境进行了对比分析,认为螨在肺螨病患者工作环境中的分布和肺螨的生境基本上相符合,因此证实了患者的病原体是直接来源于工作环境。     

肝脏移植术后肺部感染病原体调查及危险因素分析

目的　总结肝移植术后肺部感染的病原体分布特点并探讨其危险因素。方法　对我院1999年 11月至 2 0 0 3年 6月间的 130例肝脏移植进行前瞻性调查 ,统计其发病率、病死率 ,描述其时间分布、病原学分布 ,利用非条件Logistic回归方程筛选术后肺部感染危险因素。结果　肝移植术后肺部感染发病率为 32 .31% ,病死率为 35 .71%。 78.5 7%的肺部感染发生在术后第一周。革兰氏阴性菌是最常见肺部感染病原体且多高度耐药。肝移植术后肺部感染危险因素包括 :术后使用呼吸机≥ 2d、长时间手术、纤支镜检查或治疗、术前大量腹水、术后气管切开、术后肾功能异常、术后肺水肿、术后长时间留置胃管、术中长时间低血压。结论　肝移植术后肺部感染多发生在移植术后早期 ,具有高发病率、病死率高的特点。病原体多为具耐药性的革兰氏阴性菌。围手术期多方面因素均可能成为肺部感染的诱因 ,其防治工作必须贯穿整个围手术期     

长链核酸扩增技术评价病毒灭活效果的研究

目的:探讨用长链核酸扩增技术评价病毒灭活效果的可行性.方法:针对伪狂犬病毒(pseudorabies virus,PRV)糖蛋白gD基因前后的保守区设计预计产物长短不一的5对引物,用半巢式PCR技术扩增经低pH法(4.0±0.1)、巴氏消毒法[(60±1.0)℃]和s/D法(有机溶剂/洗涤荆)处理后的PRV核酸,同时以细胞感染法做平行对照.结果:低pH对PRV核酸有破坏作用,处理时间越长,核酸损伤程度越明显,处理60 min时,6.62 lgTCID50的PRV完全被灭活.5条不同长度PCR扩增产物中,只有3.9 kb的长片段检出与细胞培养结果一致.7.25 lgTCID50的PRV经(60±1.0)℃处理20 min后即被完全灭活,7.13 lgTCID50的PRV经s/D法处理1 h后被完全灭活,但各长度核酸片段扩增均为阳性,与细胞感染试验结果不符.结论:低pH对PRV核酸的损伤程度随处理时间的延长而增加;用长链PCR(3.9 kb)技术来评价经低pH法灭活病毒的效果是可行的,而该法不适合评价巴氏消毒法和S/D法灭活病毒的效果.     

Immunoblotting human C4 bound to human erythrocytes in vivo and in vitro.

A study of C4 bound to human erythrocytes in vitro and in vivo has been made by immunoblotting with mouse monoclonal anti-C4c and anti-C4d and human polyclonal anti-C4d (Rodgers and Chido) following SDS-PAGE. Multi-banded patterns differentiated between C4A and C4B isotypes. Treatment of EC4b with trypsin eliminated immunoblotting but not agglutination reactions. Serum inactivation (factor I) of EC4b resulted in banding patterns similar to those obtained from patients' EC4d. Treatment of EC4b membranes with NH2OH affected many of the bands, two were lost, one was markedly reduced and others had altered SDS-PAGE mobility. Interpretation of the bands has been made in terms of C4-acceptor complexes and inactivation fragments of C4. A distinct difference in the banding of C4A and C4B isotypes has been detected.     

病原生物学多媒体网络互动教学实验室的建设与展望

介绍了病原生物学多媒体网络互动教学实验室的建设和应具备的功能 ,论述了多媒体互动网络的建成对病原生物学实验教学环境和实验教学效果的积极促进作用 ,并对实验室以后的发展方向进行了展望     

解毒降浊、益气活瘀是治疗老年缺血性卒中的主要治则

老年缺血性卒中在病因病机、治疗方法等方面迥异于老年前期缺血性卒中患者。根据老年人的生理病理特点结合临床实际，认为正虚血瘀为本，浊毒内蕴为标，毒损脑络为老年脑缺血损伤的终结；正虚血瘀、浊毒内蕴是老年脑缺血损伤的根本原因；解毒降浊、益气活血是治疗老年缺血性卒中的重要方法之一。     

Proteolytic artifacts in SDS-PAGE analysis of selected periodontal pathogen:

The aim of the study was to examine whether proteolytic artifacts, which result in a loss and poor resolution of protein bands, occur during sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis analysis of cellular proteins from selected proteolytic ( Porphyromonas gingivalis, Prevotella nigrescens and Treponema denticola ) and non-proteolytic ( Fusobacterium nudeatum ) bacteria. Conditions to limit or prevent proteolysis were also investigated. Bacterial cells were incubated in solubilizing buffer (SDS +β mercaptoethanol) at room temperature for various periods of time before boiling. A control assay consisted of trichloroacetic acid-treated bacterial cells. Cellular proteins were separated by electrophoresis and stained with Coomassie blue. Proteolysis occurred very rapidly in the case of P. gingivalis (<30 s), whereas a longer incubation time (>1 h) was required to observe similar effects in P. nigrescens and T. denticola. No proteolysis was observed for F. nudeatum. In all cases, heat (100°C) and low pH (<4) treatments of bacterial cells could avoid production of proteolytic artifacts. Incorporation of specific protease inhibitors before solubilization of bacteria could also prevent proteolysis. More particularly, N -α- p -tosyl- l -lysine chloromethyl ketone (TLCK), iodoacetamide and diisopropylfluorophosphate (50 mM) were highly efficient for P. gingivalis, P. nigrescens and T. denticola , respectively. When outer membranes of P. gingivalis were prepared in the presence of TLCK, numerous additional protein bands, not seen in the absence of TLCK, were detected. The present study suggests that specific protease inhibitors, effective in preventing proteolysis. should be identified and added during cell fractionation and protein purification procedures.