Although the carbendazim is widely used to manage spot blight in celery cultivation, information on residues identified is of interest. In this study, we examined the dissipation and residual amounts of carbendazim in celery and soil under different cultivation methods when using the suggested dose and ten times of that and the bioconcentration factor of carbendazim for celery. The results showed that when celery leaves were sprayed with the suggested dose, the half-lives in a celery field and greenhouse were 2.75 days and 3.29 days, respectively. When the soil matrix was sprayed with the recommended dose before cultivation, the half-lives of carbendazim residues were 16.86 days and 11.97 days. We also conducted a long-term dietary risk assessment using the corresponding criteria. The results showed that, in China, the use of carbendazim at a dose of 0.022 g/m2 is safer and more reasonable when the harvest interval is 28 days.
BackgroundARNTL2 is a core component of the circadian clock genes and plays regulatory roles in the cell cycle and immune infiltration, but its mechanism in lung cancer (LC) remains unclear.ObjectiveTo investigate the clinical and therapeutic value of ARNTL2 in LC.MethodsThe Oncomine and Cancer Cell Line Encyclopedia (CCLE) databases were adopted for assessing the ARNTL2 expression, after which the Kaplan-Meier plotter and Gene Expression Profiling Interactive Analysis 2 (GEPIA2) databases were used to assess the correlation of ARNTL2 with prognosis. The univariate and multivariate Cox regression analyses were utilized to identify independent prognostic factors. Also, we explored how ARNTL2 expression is related to immune infiltration, and immunomodulators in non-small lung cancer (NSCLC) using the Tumor Immune Estimation Resource (TIMER) database, TISIDB database and Gene Set Enrichment Analysis (GSEA). Finally, coexpression of ARNTL2 and PD-L1 in lung adenocarcinoma (LUAD) was verified via immunofluorescence staining and COXPRESdb v7 database.ResultsOur study demonstrated a remarkable upregulated expression of ARNTL2 in multiple cell lines and cancers, including NSCLC. Prognostic analysis displayed a remarkable correlation between high ARNTL2 expression and unfavorable overall survival (OS) and first progressive (FP) survival among patients ailing from LUAD, and ARNTL2 was an independent predictor of prognosis for LUAD patients. GSEA analysis showed that overexpression of ARNTL2 was significantly linked with cell cycle and immunity. Furthermore, we reported a correlation of ARNTL2 expression with immunomodulators and lymphocytes. Immunofluorescence staining revealed that ARNTL2 and PD-L1 were elevated relative to normal tissue for LUAD, and colocalization of them was observed.ConclusionElevated ARNTL2 expression in LUAD revealed the prognostic values and its prospective role as a target for cell cycle and immune therapy. 相似文献
This study was conducted to determine the occurrence of the natural steroid hormones estrone (E1), 17α-estradiol (αE2), 17β-estradiol (βE2) and estriol (E3) in 38 commercial milk samples obtained from markets in Beijing, China. Liquid Chromatography coupled with tandem mass spectrometry (LC–MS/MS) was employed to determine estrogens levels. The concentrations of E1, αE2, βE2 and E3 in different milk products varied from 0–146.12 ng/L, 0–70.12 ng/L, 0–31.85 ng/L to 0–2.18 ng/L, respectively. We compared exposures to estrogens through milk consumption with acceptable daily intakes (ADIs) and threshold for toxicological concern (TTC) to determine whether estrogen intakes from milk consumption are larger or smaller than the toxicity-based benchmarks. The combined margin of safety MOS (MOST) for total estrogens are about 72–99, 118–161, 539–1104, for 2–4, 4–7 year-old residential children, and adults, respectively. The lowest MOST for children of 2–4 years old result from comparing total of estrogens with the lowest TTC value (0.15 μg/person/day) (MOS = 3.5). The MOS values suggest that the individual and total estrogens that may present in milk are not causing a health risk for the local residents, including young children. 相似文献
正The molecular pathogenesis of T-2toxin-induced cartilage destruction has not been fully unraveled yet.The aim of this study was to detect changes in serum metabolites in a rat anomaly model with articular cartilage destruction.Thirty healthy male Wistar rats were fed a diet containing T-2 toxin(300 ng/kg chow)for 3 months. 相似文献