RMP基因在体内对肝癌细胞株SMMC-7721的生长抑制作用

目的探讨RMP基因在体内抑制肿瘤细胞生长的作用及其机制。方法通过体外构建真核表达载体pGPU6-Neo-RMP-484、pFLAG-CMV-4-RMP以及稳定表达细胞株shRNA-SMMC-7721、RMP-SMMC-7721,并测定RMPmRNA表达的变化,荷瘤裸鼠皮下注射各组肝癌细胞,观察移植瘤的大小及病理变化。结果成功构建了真核表达载体及稳定表达细胞系。实验组移植瘤体积、质量、病理及肿瘤相关蛋白的表达量均有明显变化。结论RMP基因在体内能有效地抑制肝癌细胞的生长,为RMP对肝癌的基因治疗/基因干预提供了有价值的靶点及体内分子机制的实验依据,为深入研究奠定了基础。。     

过氧化物酶增殖物激活受体激动剂(罗格列酮)在大鼠急性坏死性胰腺炎中的防治作用

目的:研究PPAR-γ增殖物激动剂(罗格Yqm)对ANP及合并肺损伤大鼠是否有保护作用.方法:50只健康雄性SD大鼠随机分为5组,正常对照组、假手术组、ANP组、罗格列酮预防组,罗格列酮治疗组.正常对照组,未做任何处理;假手术组,腹腔内注射同等剂量生理盐水;采用大剂量精氨酸腹腔注射诱导ANP模型,预防组于造模前60 min给予罗格列酮10ms/kg,2次,每次间隔30 min,治疗组于造模后60 min给予罗格列酮10 ms/kg,2次,每次间隔30 min.各组于造模后24 h处死大鼠,观察大鼠血清AMY、TNF-α和IL-1β水平变化,同时检测胰腺和肺的组织病理变化和肺组织髓过氧化物酶(MPO)的变化.结果:ANP组AMY、TNF-α、IL-1β和MPO均较对照组和假手术组明显升高(P<0.001);罗格列酮预防和治疗组血清AMY、TNF-α、IL-1β、肺组织中MPO水平较ANP组明显下降,且胰腺和肺的病理损伤也明显减轻(P<0.001)结论:罗格列酮能明显降低ANP的炎症,能明显减少TNF-α、IL-1β的产生,能降低肺组织中MPO含量,对ANP及合并的肺损伤具有保护和治疗作用.     

多层螺旋CT血管造影与DSA诊断颅内动脉瘤的效果比较

目的:通过与数字减影血管造影(DSA)和外科手术结果对比,评价16层螺旋CT血管造影(CTA)对颅内动脉瘤诊断的准确性?方法:对356例临床怀疑颅内动脉瘤患者的16层螺旋CTA图像与DSA和外科手术结果进行回顾性对比研究?结果:结合CTA?DSA和术中所见,在356例患者中的195例患者颅内发现215个动脉瘤?5个动脉瘤CTA漏诊,9个动脉瘤在DSA检查中未能清楚显示,但被CTA发现,其中5个得到手术证实,另外4个未行手术治疗?16层螺旋CTA对于动脉瘤检出的敏感性与DSA的结果差别没有统计学意义?结论:16层螺旋CTA对于颅内动脉瘤检出具有很高的敏感性?特异性和准确性,在颅内动脉瘤的筛选?诊断和治疗方案的制定方面可以替代常规DSA检查?     

特发性血小板减少性紫癜患者CD3^＋、CD4^＋和CD8^＋T淋巴细胞凋亡率的研究

目的:通过检测CD3+、CD4+、CD8+T淋巴细胞的凋亡率,探讨T淋巴细胞凋亡在特发性血小板减少性紫癜(ITP)免疫发病机制中的作用。方法:应用流式细胞仪检测ITP患者外周血CD3+、CD4+、CD8+T淋巴细胞的凋亡率;分离ITP患者和正常人外周血单个核细胞(PBMC),分成A、B 2组,A组加入白介素2(IL-2),B组加入IL-2和地塞米松共培养,分别于24和48 h收获细胞,应用流式细胞仪检测CD3+、CD4+、CD8+T淋巴细胞的凋亡率。结果:①ITP患者组CD3+、CD4+、CD8+T淋巴细胞凋亡率均明显低于正常对照(均P<0.01);②细胞培养24 h时ITP患者组A、B 2组间CD3+、CD4+、CD8+T淋巴细胞凋亡率间均差异无统计学意义(均P>0.05),而48 h时B组CD3+、CD4+、CD8+T淋巴细胞的凋亡率均明显高于A组(P<0.05或0.01);正常对照组24和48 h B组CD3+、CD4+、CD8+T淋巴细胞凋亡率均明显高于A组(均P<0.05);③ITP患者组细胞培养24 h后A、B 2组间CD3+、CD4+、CD8+T淋巴细胞凋亡率的差值均明显低于正常对照组(P<0....     

The Prevailing Preference for Left Nephrectomy in Living Donor Transplantation Does Not Adversely Affect Long-Term Donor and Recipient Outcomes

BackgroundIn living donor renal transplantation, surgeons traditionally prefer the left kidney for donation. The aim of this study was to assess the effects of the choice of laterality of donor nephrectomy on donor and recipient outcomes.MethodsThe data was obtained from the UK National Health Service Blood and Transplant (NHSBT). During the study period, 7919 donor nephrectomy and transplantation were carried out in 24 transplant centers. Of these procedures, 6407 (80.9%) were left and 1512 (19.1%) were right kidney donors.ResultsRight kidney donation was associated with higher incidence of surgical site infection in the donor. Recipient outcome was superior for left-sided kidneys in terms of immediate graft function, delayed graft function, graft loss within 30 days, and graft survival at 3 years, but not at 1 and 5 years. Open donor nephrectomy (n = 2396, 30.2%) was associated with higher rates of pneumothorax and hemorrhage, longer hospital stay, and inferior graft survival at 3 and 5 years compared with laparoscopic donor nephrectomy (n = 5523, 69.8%).ConclusionsA right donor nephrectomy is associated with higher rate of wound infection in the donor and similar long-term graft outcomes in the recipients. Laparoscopic donor nephrectomy offers lower rate of major complications in the donor and a better overall graft survival.     

甘精胰岛素经皮超声导入与皮下注射的疗效比较

目的 探讨甘精胰岛素经皮超声导入的降糖效果。方法将43例T2DM患者随机分组，分别采用经皮超声导人甘精胰岛素和皮下注射甘精胰岛素两种不同方法控制血糖，自身前后交叉对照，评价经皮超声导入甘精胰岛素的降糖效果，比较不同给药方式所需的胰岛素剂量。结果甘精胰岛素采用经皮超声导入和皮下注射两种不同的给药方式，对空腹及餐后血糖的控制无统计学差异（P〉0．05），不同给药方式所需甘精胰岛素剂量具有统计学差异（P〈0．05）。结论经皮超声导入甘精胰岛素以及皮下注射甘精胰岛素两种给药方式均能有效控制血糖。在达到同样降糖目标的情况下，经皮超声导入所需甘精胰岛素剂量较皮下注射增多15％左右。     

The Reliability and Validity of Post Stroke Depression Scale in Different Type of Post Stroke Depression Patients

ObjectiveA self-rating post stroke depression scale (PSDS) showed a good reliability and validity to assess severity of depressive symptoms among stroke patients. This study aimed to retest the psychometric properties of PSDS in different types of post-stroke depression (PSD).Materials and methodsA total of 170 stroke patients were recruited in the study. 82 and 25 patients were respectively diagnosed as PSD symptoms disorder (PSDSD) and PSD disorder (PSDD) patients according to their respective diagnostic criteria. The PSDS and the 9-item Patient Health Questionnaire (PHQ-9) were used to assess the severity of depression. Cronbach α, Spearman rank coefficient and independent sample t-test were conducted to examine reliability, internal consistency and discriminate validity. Then the receiver operating characteristic curve and Youden index were used to performance evaluation and cut-off value respectively in different subtypes of PSD patients.ResultsThe Cronbach α of PSDS was 0.857, indicting a good reliability. The Spearman correlation coefficient between PSDS and PHQ-9 was 0.942 (P<0.001). The discriminate validity displayed significant difference between PSDSD as well as PSDD and no depression patients (all P<0.001). 5/24 and 10/24 were the cut-off value for PSDSD and PSDD patients.ConclusionsPSDS is a useful screen tool with an acceptable psychometric properties for estimation of different subtypes of PSD patients.