胎儿股骨骺板和干骺端胰岛素样生长因子－Ⅰ分布的研究

采用硬组织切片技术和ＡＢＣ免疫组织化学技术，对５例１６～２０周胎儿股骨骺板、干骺瑞进行胰岛素样生长因子－Ｉ（简称ＩＧＦ－Ｉ）的定位研究。观察到骺板储备区、增生区和肥大区均有ＩＧＦ－Ｉ免疫反应阳住的软骨细胞；干骺端存在一定数量的ＩＧＦ－Ｉ阳性成骨细胞，部分类骨质和骨基质也有阳性反应物沉积，少数破骨细胞也呈ＩＧＦ－Ｉ免疫阳性反应。作者同时讨论了在硬组织切片上进行免疫组化染色的可能性和ＩＧＦ－Ｉ在骨组织内的作用。     

显微锁孔手术治疗脑干及其周围病变

目的 将显微锁孔手术应用于脑干及其周围病变的外科治疗，探求以最小的创伤来取得最佳的手术疗效。方法 采用颞下锁孔人路、乳突后锁孔人路、枕下正中锁孔人路，以20mm左右直径的骨窗进行脑干及其周围病变的显微手术治疗。结果 本组16例例病人术后3d内均行MRI或DSA检查，肿瘤或动静脉畸形全切除11例，次全切除3例，部分切除1例，1例小脑后下动脉瘤成功夹闭。术中输血3例。并发脑脊液耳漏1例，硬膜下积液1例，1例术后持续昏迷40d苏醒，无死亡及感染病例。结论 锁孔人路微创技术处理脑干及其周围病变，因其手术创伤小，疗效佳，费用节省，值得临床推广应用。     

IMMUNOREGULATORY EFFECT OF PANAXATRIOL GINSENOSIDE ON IMMUNE FUNCTIONS IN BONE MARROW SUPPRESSED MICE

The effect of Panaxatriol Ginsenoside (PTGS) on Immune functions in bone marrow suppressed mice induced by injection of cyclophosphamide (CY) has been studied. Bone marrow suppressed mice were made by injection of CY (150 mg/kg) parenterally. Subcutaneous injection of PTGS three days earlier partially restored the number and the activity of bone marrow cells, significantly enhanced the production of IL-1, IL- 3 and IL- 6 like substances and promoted the reactivity of murlne spleen cells to Con-A In bone marrow suppressed mice.     

STUDIES ON NUTRITION AND PRECANCEROUS LESIONS OF THE ESOPHAGUS IN THE ADOLESCENTS

This paper reports the prevalence of chronic esophagitis and nutritional status among 538 young persons aged 15 to 26 years from the high risk area for esophageal cancer. Of these subjects, 166 were from households with history of esophageal cancer and 372 were from households without history of esophageal cancer. The Incidences of chronic esophagltis among male and female adolescents were 37. 6% and 36% respectively, which was significantly higher than those in the low risk area (17%). The frequency of chronic esophagltis in the adolescents in the households with history of esophageal cancer was aiso higher than in those In the households without history of esophageal cancer. The deficiencies of vitamins, especially of riboflavin and ascorbate, are prevalent and severe among these adolescents. Ascorbate deficiency Is correlated with the severity of the chronic esophagltis. These results indicate that chronic esophagltis may be involved in the natural history of esophageal carclnogenesis. Nutrient defic     

Inhibitory effect of glycyrrhiza uralensis fish and chelidonium majus L on gastrocarcinogenesis induced by N-methyl-N′-nitron-N-nitrosoguanidine

In order to investigate the antagonistic effect of Glycyrrhiza Uralensis Fish (GUF) and Chelidonium maJus L (CML) on gastrccarcinogenesis induced by MNNG in Wastar rats, we treated the rats with MNNG alone (group 1) and with MNNG plus GUF and CML (group 2 and 3) respectively. The incidence of infiltrating adenocarcinoma of the glandular stomach and duodenum in group 2 was significantly lower than that in group 1 (26.7% vs. 67.8%). The differentiation and aggressivenees of carcinomas occured in group 2 were much better and mild than those in group 1. Present study also demonstrated that the inhibitory effect of CML on proliferation of human stomach carcinoma cell line MGC-803 was very remarkable; in addition, GUF and CML were able to antagonise the mutagenic activation of MNNG. These results suggest that GUF and CML may be empoyed in prevention of gastric carcinoma.     

一种鉴别肥大细胞表型的复合染色法

运用ABC-爱先蓝-PAS复合染色法,分别对大鼠肝,胃、肠中不同表型肥大细胞即结缔组织肥大细胞(CTMC)和粘膜肥大细胞(MMC)进行鉴别染色实验。结果表明:肝汇管区MC呈棕褐色;胃粘膜上皮及粘膜下结缔组织内MC分别被染成蓝色和棕褐色;肠粘膜固有层MC呈蓝色.通过不同颜色反应从而确定其MC表型。本实验还通过对染色顺序。固定液选择等的多次摸索。取得了较好的染色效果,与其它检测方法的结果相一致,但试剂来源方便,操作简易。一般实验室即可进行。     

人胎盘微绒毛膜的制备及其转铁蛋白受体研究

作者分别用差速离心法及蔗糖梯度离心法制备胎盘微绒毛膜(PMM),用受体放射分析法研究了PMM的转铁蛋白受体(TfB),结果表明:两种方法制备的PMM均可用于受体分析,但差速离心法更为简便。测定60例产妇PMM的TfR,其数目为3.53±1.98×10~(12)个位点/mg膜蛋白,最大结合容量为6.33±4.21×10~(-12)mol/mg膜蛋白,Kd值为4.95±3.39×10~(-9)mol/L。受体结合反应体系最适实验条件为,膜蛋白浓度每管50μg,标记物浓度每管50 000cpm,孵育30分钟,B/F分离的聚乙二醇浓度为12％(W/V)。对TfR的特性研究表明:TfR与转铁蛋白的结合具有高度亲和力、高度特异性及可饱和性。     

50例冠心病某些生化基础观测的临床诊断意义

对５０例冠心病患者进行某些生化基础观测，发现冠心病肾阴虚与糖耐量减低有关，肾阳虚患者尿１７－羟低於正常；而冠心病标证有其生化基础，与甘油三酯增高有密切相关。     

我国登革2型病毒43株包膜E蛋白基因的特征

对我国1987年流行的登革2型病毒43株包膜E蛋白基因的核苷酸序列进行了分析。结果表明登革2型病毒43株包膜E蛋白基因核苷酸序列含1485个核苷酸，编码495个氨基酸，并就其核苷酸序列及其相应的氨基酸序列与其它的登革2型病毒株进行了比较，发现核苷酸序列与我国1985年分离的登革2型病毒04株，新几内亚C株（NGC），牙买加株1409（JAM）和马来西亚当地流行株M1（登革出血热）、血2（登革休克综合征）、M3（登革热）同源性分别是95.8%、94.6%、97.5%、925%、92.7%和939%，氨基酸序列的同源性分别是94.3%、94.3%、96.0%、93.7%、93.7%和91.5%，推断出的氨基酸序列显示出12个保守的半胱氨酸残基和两个潜在的糖基化位点，分别位于Asn-67和Asn-153位。     

X线录相对无喉人食管发音机理的初步探讨

本文对19名无喉人食管发音患者,利用X线电影、X线录相及录音技术,对食管发声的机理进行了图、声同步的动态观察与分析。认为食管发声须经过:科学训练;代声门形成;贮气、送气、发音三个时相过程及口腔、鼻腔、舌唇、齿等构字器官的协调配合加工而成。文中还对此项检查设备、方法、检查前准备及步骤进行了详尽论述。