心肌梗死后移植骨髓间充质干细胞后全身细胞的分布

Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs) 24 h and 2 weeks after cell injection into the border zone of myocardial infarction area. Methods MSCs from male SD rats were labeled with Bromodeoxyuridine (BrdU). Three weeks after in-duction of myocardial infarction,female SD rats were randomized into 2 groups. Labeled cells (3 × 10 ,50 μl) were injected into the border zone of infarcted area in the one group (n = 12) ,and PBS of equal vol-ume was injected into the border zone of infarcted area in the other group ( n = 8 ). The systemic distribu-tion of MSCs was evaluated through real-time PCR and immunohistochemistry at time points of 24 h and 2 weeks after injection, respectively. Results Cells injected into border zone of infarcted area were distribu-ted to extra-cardiac organs such as spleen,lung and liver. Twenty-four h after injection,cells mainly con-centrated in the heart (467 467 ± 191 387) ,while obvious cell loss was noted in all organs including the heart ( 112 388 ±43 751 ) 2 weeks after injection. Of immunostaining were consistent with those of real-time PCR. Conclusion After injected into the border zone of infarcted area, MSCs mainly gathered in the heart with distributions into spleen, lung, and liver. However, substantial number of cells lost with pro-longed time span.     

子宫内膜增生患者的中医临床特点分析

【目的】 总结分析子宫内膜增生患者的中医临床特点。【方法】 采用回顾性调查方法,收集2016年5月至2020年5月期间在广东省中医院珠海医院妇科因“异常子宫出血”住院治疗,经宫腔镜检查及诊刮,病理诊断为子宫内膜增生不伴不典型增生（EH）或子宫内膜不典型增生（AH）的患者,共 112 例。其中 EH 组 90 例,AH 组 22 例;而 EH 组中,单纯性增生组73 例,复杂性增生组17例。采集患者的发病年龄、病程、中医四诊资料、宫腔镜下表现、合并疾病、治疗转归等情况,并分析其中医辨病情况。【结果】（1）一般资料方面,子宫内膜增生患者的发病年龄为（41.3 ± 6.9）岁,中位病程为3个月,不同病理类型子宫内膜增生患者的发病年龄和病程分布情况比较,差异均无统计学意义（P＞0.05）。（2）中医四诊资料方面,主症（月经情况）多见漏下淋漓[占47.3%（53/112）]、崩中暴下[占12.5%（14/112）]、经期延长[占12.5%（14/112）],而经血的色质变化多不典型;兼证常见腰膝酸软[占28.6%（32/112）]、头晕乏力[占14.3%（16/112）]、小腹隐痛[占8.0%（9/112）];舌质以淡黯或红多见,而舌苔及脉象较繁杂。除AH组的崩中暴下发生率[35.0%（7/22）]明显高于EH组[7.8%（7/90）],差异有统计学意义（P＜0.01）外,不同病理类型子宫内膜增生患者的其他中医四诊资料比较,差异均无统计学意义（P＞0.05）。综合中医四诊资料,其辨证以肾虚肝郁、血瘀气滞为主,出血过多时可伴血虚。（3）宫腔镜下表现方面,主要为内膜增厚,局限增生,血管丰富、扩张等,部分患者伴有内膜息肉形成[占50.9%（57/112）]。（4）合并疾病方面,合并子宫肌瘤者占34.8%（39/112）,合并子宫腺肌病者占12.5%（12/112）,合并乳房结节者占52.7%（59/112）,未见乳房恶性肿瘤。并发贫血者占41.1%（46/112）,其中发展至重度贫血者占7.1%（8/112）。（5）辨病方面,居前3位者分别为崩漏、经期延长、癥瘕,不同病理类型子宫内膜增生患者的辨病情况比较,差异无统计学意义（P＞0.05）。【结论】 子宫内膜增生患者的中医证候在EH、AH间无明显差异,其辨证以肾虚肝郁、血瘀气滞为主,出血过多时可伴血虚。     

心肌梗死后移植骨髓间充质干细胞后全身细胞的分布

Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs) 24 h and 2 weeks after cell injection into the border zone of myocardial infarction area. Methods MSCs from male SD rats were labeled with Bromodeoxyuridine (BrdU). Three weeks after in-duction of myocardial infarction,female SD rats were randomized into 2 groups. Labeled cells (3 × 10 ,50 μl) were injected into the border zone of infarcted area in the one group (n = 12) ,and PBS of equal vol-ume was injected into the border zone of infarcted area in the other group ( n = 8 ). The systemic distribu-tion of MSCs was evaluated through real-time PCR and immunohistochemistry at time points of 24 h and 2 weeks after injection, respectively. Results Cells injected into border zone of infarcted area were distribu-ted to extra-cardiac organs such as spleen,lung and liver. Twenty-four h after injection,cells mainly con-centrated in the heart (467 467 ± 191 387) ,while obvious cell loss was noted in all organs including the heart ( 112 388 ±43 751 ) 2 weeks after injection. Of immunostaining were consistent with those of real-time PCR. Conclusion After injected into the border zone of infarcted area, MSCs mainly gathered in the heart with distributions into spleen, lung, and liver. However, substantial number of cells lost with pro-longed time span.     

心肌梗死后移植骨髓间充质干细胞后全身细胞的分布

Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs) 24 h and 2 weeks after cell injection into the border zone of myocardial infarction area. Methods MSCs from male SD rats were labeled with Bromodeoxyuridine (BrdU). Three weeks after in-duction of myocardial infarction,female SD rats were randomized into 2 groups. Labeled cells (3 × 10 ,50 μl) were injected into the border zone of infarcted area in the one group (n = 12) ,and PBS of equal vol-ume was injected into the border zone of infarcted area in the other group ( n = 8 ). The systemic distribu-tion of MSCs was evaluated through real-time PCR and immunohistochemistry at time points of 24 h and 2 weeks after injection, respectively. Results Cells injected into border zone of infarcted area were distribu-ted to extra-cardiac organs such as spleen,lung and liver. Twenty-four h after injection,cells mainly con-centrated in the heart (467 467 ± 191 387) ,while obvious cell loss was noted in all organs including the heart ( 112 388 ±43 751 ) 2 weeks after injection. Of immunostaining were consistent with those of real-time PCR. Conclusion After injected into the border zone of infarcted area, MSCs mainly gathered in the heart with distributions into spleen, lung, and liver. However, substantial number of cells lost with pro-longed time span.     

心肌梗死后移植骨髓间充质干细胞后全身细胞的分布

Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs) 24 h and 2 weeks after cell injection into the border zone of myocardial infarction area. Methods MSCs from male SD rats were labeled with Bromodeoxyuridine (BrdU). Three weeks after in-duction of myocardial infarction,female SD rats were randomized into 2 groups. Labeled cells (3 × 10 ,50 μl) were injected into the border zone of infarcted area in the one group (n = 12) ,and PBS of equal vol-ume was injected into the border zone of infarcted area in the other group ( n = 8 ). The systemic distribu-tion of MSCs was evaluated through real-time PCR and immunohistochemistry at time points of 24 h and 2 weeks after injection, respectively. Results Cells injected into border zone of infarcted area were distribu-ted to extra-cardiac organs such as spleen,lung and liver. Twenty-four h after injection,cells mainly con-centrated in the heart (467 467 ± 191 387) ,while obvious cell loss was noted in all organs including the heart ( 112 388 ±43 751 ) 2 weeks after injection. Of immunostaining were consistent with those of real-time PCR. Conclusion After injected into the border zone of infarcted area, MSCs mainly gathered in the heart with distributions into spleen, lung, and liver. However, substantial number of cells lost with pro-longed time span.     

高月平治疗子宫内膜异位症经验

高月平是南京中医药大学中医妇科学教授,从事中医妇科教学、临床工作20余年,学验俱丰。笔者在随其学习期间,观察到高教授对子宫内膜异位症的治疗见解独到,疗效甚佳,现归纳介绍如下。1发病机制子宫内膜异位症(内异症)是现代生育期妇女的常见病、多发病,现多根据其症状、体征将其     

XELOX方案联合恩度一线治疗晚期胆系肿瘤的临床观察

目的 观察卡培他滨和奥沙利铂（XELOX方案）联合重组人血管内皮抑制素（恩度）一线治疗晚期胆系肿瘤的临床疗效及毒副反应。方法 收集2008年1月至2013年12月我院肿瘤科收治的胆系肿瘤Ⅳ期患者42例,随机分为联合组（n=18）和单纯化疗组（n=24）。单纯化疗组应用XELOX方案化疗：卡培他滨1.25 g/m2 口服,d1~d14;奥沙利铂85 mg/m2 静脉滴注,d1。联合组在应用以上药物的同时给予恩度15 mg静滴3~4 h,d1~d14。21 d为1个周期,每2个周期评价疗效、生活质量（QOL）及毒副反应,比较两组有效率（RR）、疾病控制率（DCR）、中位无疾病进展生存期（mPFS）和中位生存时间（mOS）。结果 单纯化疗组患者获CR 0例,PR 6例,SD 8例,PD 10例,RR为25.0%,DCR为58.3%;mPFS为 5个月,mOS 为9.5个月,QOL改善稳定率为66.7%。联合组患者获CR 0例,PR 5例,SD 6例,PD 7例,RR为27.8%,DCR为61.1%;mPFS为7.5个月,mOS为14个月,QOL改善稳定率为77.8%。两组mPFS、mOS和QOL改善稳定率比较,差异均有统计学意义（P均<0.05）。两组毒副反应主要为消化道反应、手足综合征、骨髓抑制、神经毒性及口腔黏膜炎,多为Ⅰ级/Ⅱ级,Ⅲ级/Ⅳ级少见,差异均无统计学意义（P均＞0.05）。结论 XELOX方案联合恩度一线治疗晚期胆系肿瘤的疗效较好,毒副反应可耐受,安全性良好,值得进一步观察及临床推广应用。     

转基因雌鼠乳腺癌大豆异黄酮干预效果评价

目的探讨大豆异黄酮对MMTV-erbB-2转基因雌性小鼠乳腺癌发生、发展的影响。方法随机将100只MMTV-erbB-2转基因雌性小鼠分为早期干预组、晚期干预组、全程干预组和对照组,每组各25只,观察各组小鼠乳腺癌的发病率、潜伏期、肿瘤生长部位及肿瘤生长速度。结果早期干预组、晚期干预组、全程干预组和对照组雌性小鼠乳腺癌的发病率分别为48%,28%,20%和92%;与对照组比较,其他3组发病率均降低(P0.01);全程干预组发病率低于早期干预组(P0.05);晚期干预组与早期干预组和全程干预组比较,发病率差异均无统计学意义(P0.05);各组肿瘤发生部位主要在小鼠3对乳腺;各组小鼠乳腺癌潜伏期和肿瘤生长速度间差异均无统计学意义(P0.05)。结论大豆异黄酮对MMTV-erbB-2转基因雌性小鼠乳腺癌的发生、发展具有一定预防作用。     

食管鳞癌及其癌前病变组织p63与Ki-67蛋白共表达临床意义的探讨

目的:研究食管癌高发区食管鳞癌组织及癌前病变标本中p63和Ki-67蛋白的联合表达,探讨其共表达与食管癌变的关系及作为早期癌变生物学标志的可能性.方法:应用免疫组化技术对来自食管癌高发区的正常食管黏膜组织和轻度、中度、重度不典型增生上皮以及癌组织共203例组织中p63和Ki-67蛋白的异常表达进行研究.结果:在正常黏膜、轻度、中度、重度不典型增生及癌组织中,p63蛋白异常表达的阳性率分别为15.0%(3/20)、36.8%(14/38)、47.1%(24/51)、65.2%(30/46)和83.3%(40/48);Ki-67异常表达检出率分别为10.0%(2/20)、28.9%(11/38)、41.2%(21/51)、56.5%(26/46)和81.3%(39/48),等级相关分析结果显示,p63、Ki-67表达异常与组织学分级均显著相关(r分别为0.553和0.583,P<0.01),而且p63蛋白与Ki-67蛋白表达之间也具有相关性(r=0.690,P<0.01).结论:p63和Ki-67蛋白共表达与食管癌癌变过程显著相关,p63及Ki-67表达改变的时相分布,有可能成为在食管癌前人群中确立高危个体和选择重点化学预防个体的分子生物学标志.