Fc receptor-mediated accumulation of macrophages in crescentic glomerulonephritis induced by anti-glomerular basement membrane antibody administration in WKY rats

Anti-glomerular basement membrane (GBM) glomerulonephritis induced in WKY rats is characterized by glomerular accumulation of CD8(+) T cells and monocytes/macrophages, followed by crescent formation. The mechanism of leukocyte accumulation after antibody binding to GBM is still unclear. To unveil an involvement of Fcgamma receptors (FcgammaR) in leukocytes recruitment we examined the expression of FcgammaR in glomeruli and the effects of the administration of F(ab')(2) fragment of anti-GBM antibody or FcgammaR blocking on the initiation and progression of this model. A gradual increase of FcgammaR mRNA expression in glomeruli during the time course of disease suggested their significance in the development of glomerulonephritis. Glomerular lesions and proteinuria were induced only in rats injected with intact IgG of anti-GBM antibody, but not with the F(ab')(2) fragment. In vivo blocking of FcgammaR by administering heat-aggregated IgG led to the decrease of mRNA expression for all types of FcgammaR (types 1, 2 and 3) and a significant amelioration of glomerulonephritis manifestations. By flow cytometry and immunohistochemistry FcgammaR2-expressing cells in glomeruli were identified as macrophages, but not CD8(+) T cells. The expression of FcgammaR1 and 3 was significantly decreased, and that of FcgammaR2 became undetectable in CD8(+) T cell-depleted rats. Thus, CD8(+) T cells may stimulate FcgammaR expression on macrophages, contributing to their glomerular accumulation and injury. These studies provide direct evidence for a crucial involvement of IgG Fc-FcgammaR interaction in glomerular recruitment of macrophages and following induction of anti-GBM glomerulonephritis in WKY rats.     

Functional polymorphisms in cell death pathway genes FAS and FASL contribute to risk of lung cancer

Background: The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. There is reduced expression of FAS but elevated expression of FASL in many types of human cancers including lung cancer. We recently reported an association between functional polymorphisms in FAS (–1377G→A) and FASL (–844T→C) and risk of oesophageal cancer.     

血糖感应型胰岛素给药智能载体的研究进展

胰岛素控制释放高分子载体系统一直是国内外科技工作者的研究热点 ,迄今已经研究报道了多种具有不同工作原理的血糖感应型胰岛素给药智能载体。本文基于国内外大量研究文献 ,综述了血糖感应型胰岛素控制释放智能化高分子载体的研究进展。     

缺血预处理对大鼠骨骼肌缺血再灌注损伤时Bax基因蛋白表达影响的实验研究

探讨缺血预处理对大鼠骨骼肌缺血再灌注损伤的可能影响,特别是对Bax基因表达的影响.用免疫组化方法检测并观察大鼠后肢止血带缺血4 h后再灌注4、24、48 h后和先经预缺血处理后再灌注4、24 h后Bax基因蛋白在肌细胞中的表达情况.结果表明缺血4 h(A组)肌细胞出现Bax蛋白表达,24h后(B组)表达量显增加,48h后(C组)无表达.缺血预处理后再灌注4 h后(D组)肌细胞内无Bax蛋白表达,再灌注24 h后(E组)Bax蛋白表达量非常显著地低于B组.Bax基因参与了骨骼缺血再灌注损伤的病理过程,其蛋白表达量与组织损伤程度密切相关,经缺血预处理可以下调Bax基因蛋白的表达水平、或是抑制其合成,使肌细胞存活而发挥其保护作用.     

电刺激杏仁核对猫丘脑后核内脏大神经诱发放电的抑制作用

本文使用条件—检验刺激技术,研究了电刺激杏仁皮质内侧核对猫丘脑后核内脏大神经诱发放电的影响。电刺激杏仁皮质内侧核抑制丘脑后核的中等和长潜伏期内脏大神经诱发放电呈现两种形式:即时抑制和迟发抑制。前者可能是通过从杏仁核到丘脑后核的神经通路产生的,静脉注射纳洛酮可消除这种抑制,提示有鸦片样物质的参与。后者可能涉及神经—体液性因素。结果提示,杏仁核参与抑制内脏痛。     

杜仲及其有效成分治疗膝骨关节炎的作用机制研究现状

膝骨关节炎（KOA）是中老年常见的退行性关节疾病,发病率随着人口老龄化程度加深及肥胖人群增加而不断增加,严重影响患者健康及日常生活。目前采用的非甾体类抗炎药、软骨保护类药物、阿片类镇痛药等对症治疗手段作用有限,且药物不良反应明显。杜仲是治疗KOA常用且有效的中药之一,但其作用机制和药效物质基础尚未明确,限制了其在临床更为广泛的运用。杜仲在KOA治疗领域的有效成分主要为环烯醚萜类（京尼平苷、杜仲苷/桃叶珊瑚苷）、木脂素类（松脂醇二葡萄糖苷）、黄酮类（槲皮素、紫云英苷、黄芩素、金丝桃苷、山柰酚）、苯丙素类（绿原酸）、杜仲多糖等化合物,他们主要通过丝裂原活化蛋白激酶、核转录因子-κB、磷脂酰肌醇3-激酶/蛋白激酶B及等Janus激酶1/信号转导和转录激活因子3等信号通路,来调节炎性因子水平、抗氧化应激反应、保护软骨细胞、平衡细胞外基质合成与降解等,控制KOA病情进展。该文对杜仲及其有效成分在KOA治疗方面的作用机制进行了综述,以期为KOA新药研发提供理论依据。     

基于内控视角的医院后勤维修改造项目管理与审计

主要就医院后勤支出中占比较高的后勤维修改造项目的管理和审计进行探讨和分析,研究如何规范管理,从内控视角出发制定一套高标准、高质量的项目全流程监管体系和审计方法,以提高医院运营效率,降低运营成本。     

乳酸及降钙素原对一氧化碳中毒后迟发性脑病的预测价值

目的探讨检测血乳酸、降钙素原和联合检测对急性一氧化碳中毒后迟发性脑病(DEACMP)的预测价值。 方法随机抽取我院在急诊科收住院的ACMP患者70例,随访3个月,以其中发生DEACMP患者10例为DEACMP组,其余60例为非DEACMP组,随机抽取同期笔者医院40例健康体检者为对照组。对患者立即行动脉血Lac和血清PCT检测,并计算Lac和PCT诊断DEACMP的灵敏度、特异度。 结果DEACMP组血Lac和PCT检测值均明显高于非DEACMP和对照组,差异有统计学意义(采用秩和检验,P<0.05);检测异常率亦明显高于非DEACMP组及对照组,差异有统计学意义(采用χ²检验,P<0.05)。Lac和PCT升高预测DEACMP的敏感度均为90.0% ,特异度分别为50.0%和65.0% ,准确性分别为55.7%和68.6%。联合检测时若其中1个指标为阳性时即预测DEACMP发病,则联合检测的敏感度为100.0%,特异度为41.7%,阳性预测值为21.7%,阴性预测值为100.0%,准确性为71.4%。 结论DEACMP患者早期动脉血Lac和PCT均明显升高,PCT诊断价值优于Lac,联合检测较单项指标检测对DEACMP发病预测价值更理想。     

益气止血药对脾不统血证模型动物前列腺素的作用

采用复合因素的脾不统血证大鼠模型 ,以 6 酮 前列腺素F1α(6 酮 PGF1α)和血栓素B2(TXB2 )作为观测指标 ,对益气止血药对脾不统血证模型大鼠血浆和子宫前列腺素的影响进行了研究。结果 :大剂量组益气止血药能降低脾不统血证模型血浆和子宫的 6 酮 PGF1α及子宫 6 酮 PGF1α/TXB2 比值。说明 6 酮 PGF1α及 6 酮 PGF1α/TXB2 比值升高可能是脾不统血证的重要机理之一。     

Cloning and identification of a novel variant of human vascular endothelial growth factor

Vascular endothelialgrowth factor ( VEGF) is amultifunctional cytokine thatexerts in vivo a key rolein physiologicalneoangiogenesisduring embryonicde-velopmentor the female cycle and pathologicalneoan-giogenesis of many diseases including hypervascular-ized tumors,rheumatoid arthritis,and retinopathiesdiseases[1— 3] by stimulating endothelial cell prolifera-tion and vessel hyperpermeability.VEGF exists asone of five different isoforms,VEGF1 2 1 ,VEGF1 65 ,VEGF1 83,VEGF1 89and VEGF2 …