腹腔镜对原发性闭经和继发性闭经的诊断价值

为探讨腹腔镜检查对原发性和继发性闭经的诊断价值。研究原发性闭经患者92例,继发性闭经患者108例,腹腔镜下观察:子宫、输卵管、卵巢及盆腔腹膜。部分患者行卵巢活检,其中19例继发性闭经合并不孕患者行输卵管通液,观察输卵管通畅情况。结果 原发性闭经患者以先天性发育异常居首位。占65.22％,继发性闭经患者以多囊卵巢综合征居首位,占52.78％,盆腔结核仍为闭经一个不可忽视的原因。临床和腹腔镜误(漏)诊率:原发性闭经分别显72.83％和4.35％,继发性闭经分别为88.99％和1.83％,经统计学处理,原发性和继发性闭经患者其临床和腹腔镜误(漏)诊率均有显著性差异,19例继发性闭经患者不孕的主要原因为卵巢功能异常。因此,大多数闭经患者可通过腹腔镜检查确定病因,少数病例尚需进行其它相关的检查。     

pH值对邻苯二亚胺纤维素的制备及荧光性能的影响

制备了席夫碱（Ｓｃｈｉｆｆ’ｓ ｂａｓｅ）邻苯二亚胺纤维素（ＰＤＩＭＣ），考查ｐＨ值对制备反应和产物荧光性能的影响。结果表明：氧化度为１００％的二醛纤维素在ｐＨ＝７￣１３、室温下、反应３０ｈ生成的邻苯二亚胺纤维素，具有强较的荧光性能，熔点４１０ｋ，分解温度为５７８Ｋ；ＰＤＩＭＣ水溶液在ＰＨ＝７．６，浓度为７．６×１０＾－４ｇ／ｍＬ，２０℃时，Ｅｘ＝４７０ｎｍ，Ｅｍ＝５５５ｎｍ处荧光最强。     

乙型肝炎后肝硬化患者外周血IL—2,IFN—γ活性与肝功能分级的关系

目的：探讨乙型肝炎后肝硬化（ＰＨＣ）患者外周血白介素２（ＩＬ－２）和γ－干扰素（ＩＮＦ－γ）生与肝功能状态的关系。方法：测定５６例ＰＨＣ患者的２４例健康成人外周血淋巴细胞ＩＬ－２受体（ＩＬ＿２Ｒ）的表达，ＩＬ－２和ＩＦＮ－γ生成及因清可溶性ＩＬ－２受体。结果：ＰＨＣ组和对照组的ＩＬ－２活性ＩＬ－２Ｒ表达率、ＩＦＮ－γ活性之间均呈正相关，与ＳＩＬ－２Ｒ水平则负相关，。与对照组比较，ＰＨＣ组ＩＬ－２Ｒ     

急性心肌梗塞后心室重构

心室重构（Ventricularremodeling）又称心脏重构、心肌重构，简单来说是指心脏结构发生了改变。它往往是充血性心力衰竭最早的解剖基础，并贯穿于整个病程发展过程中，越来越引起临床医生的关注。急性心肌梗塞（Acutemyocardialinfarction，AMI）后的心室重构，是指急性心肌梗塞后左心室梗塞区和非梗塞区结构及形态的改变，根据其发生时间及演变，可分为梗塞区伸展（Infarctexpansion）和左心室扩张（Ventriculardilation）[1]。1发病机理和病理改变梗塞区伸展，是梗塞区不成比例地拉长、变薄，使局部室壁变形，心室腔扩大，但无新的心…     

精子核DNA及形态图像定量分析与评价

精子形态观察在精液检查中占有重要位置,常规显微镜检查带有一定的主观性,我们采用国产真彩色医学图像分析仪,能精确测量精子核DNA及形态参数各指标,现将结果报告如下。 1 材料与方法 1.1 对象 取35例正常生育力男性精液,     

Increased Expression of PI-3K in Asthmatic Rat T Lymphocytes

In order to explore the expression of PI-3K in T lymphocytes of asthmatic rats and the relationship between PI-3K and activation of T lymphocytes, 24 Wistar rats were randomly divided into 4 groups: normal control group, asthmatic one-week group, asthmatic two-week group and asth-matic four-week group. T cells were purified from blood of each rat and the expression of PI-3K was observed by immunocytochemical fluorescence staining, the semiquantitative fluorescence intensity was measured by HPIAS-2000 analytic software, and the expression of IL-4 in supernatants was de-tected by ELISA. The results showed that the fluorescence intensity of T lymphocytes in asthmatic groups was significantly higher than that in normal control (P<0.001), indicating that the expression of PI-3K in T lymphocytes of asthmatic rats was significantly higher than that in those of normal controls, and the difference between acute and chronic stage asthmatic groups was significant (P<0.05). The expression levels of IL-4 protein in supernatants of asthmatic T lymphocytes were sig-nificantly higher than those in the normal controls (P<0.05). There was a significant positive correla-tion between the expression of PI-3K in T lymphocytes and the IL-4 protein expression in super-natants (r=0.583, P<0.01). It was suggested that PI-3K signal pathway may participate in the proc-esses of activation and other cytological effects of asthmatic T lymphocytes, thus may play an impor-tant roles in the pathogenesis of asthma.     

Primary Culture of Alveolar Epithelial Type Ⅱ Cells and Its Bionomic Study

To establish a better method of primary culture for alveolar epithelial type Ⅱ cells (AEC Ⅱ) and to study its bionomics, alveolar epithelial type Ⅱ cells were isolated by digestion with tryp- sin and collagenase, which were then purified by plated into culture flask coated with rat immu- noglobulin G. The purified AEC Ⅱ were identified by alkaline phosphatase staining, electron mi- croscopy, immunocytochemical staining of pulmonary surfactant protein A (SPA). The SPA expres- sion and transfection characteristics were compared with those of A549 cell line. The results showed that AEC Ⅱ could be isolated by digestion with trysin and collagenase and purified by adhesive pu- rification by using IgG, with a yield of about 2–3×107, and a purity of about 75%–84 %. Cells could be quickly identified with AKP staining. AECⅡ were different from A549 cell line in terms of SPA expression and transfection characteristics. It is concluded that adhesive purification with IgG can improve the purity of AECⅡ, and AKP staining is simple in cell identification. AECⅡ can not be completely replaced by A549 cells in some studies because the differences between them, such as SPA expression.     

难治性心力衰竭的中西医治疗进展

心力衰竭患者在经过标准的三联(地高辛、ACEI和利尿剂)治疗，对可能祛除的导致心力衰竭恶化的原因也得到纠正以后，其心力衰竭的症状仍然反复发作或持续存在，则称为顽固性心力衰竭或难治性心力衰竭(Refractory heart failure，RHF)。此类患者多是心肌病变进入了不可逆阶段。近年来西医对RHF发生发展机制有了深刻认识，但疗效并不显著，而中西医结合治疗扩宽治疗途径，成为治疗的新趋热。现就近几年的中西医治疗RHF进展作一综述。