银杏叶制剂、丹参对急性肾功能衰竭家兔血液流变学的影响

目的 :探讨银杏叶制剂 (GbE)、丹参对实验性急性肾功能衰竭(ARF)兔血液流变学、肾功能的影响。方法 :用 5 0 %的甘油 10ml/kg行兔后肢肌肉加压注射 ,建立ARF动物模型 ,观察不同时相血液流变学、肾功能 (血Cr、BUN)的改变 ,并给予GbE、丹参注射液处理。结果 :ARF组肾功能明显下降 ,血液流变学指标明显异常。GbE组、丹参组动物血液流变学指标明显改善 ;肾功能得到保护。结论 :GbE、丹参对甘油所致ARF家兔的血液流变性、肾功能有明显的保护作用     

沉默Galectin-3对人甲状腺乳头状癌细胞增殖、侵袭能力影响及机制研究

目的:探讨沉默Galectin-3对人甲状腺乳头状癌细胞增殖、侵袭能力的影响及其主要机制.方法:分为空白对照组(Control,无特殊处理)、阴性干扰组NC-siRNA(非特异性siR-NA)、RNA干扰组(人甲状腺乳头状癌细胞中转染干扰Galectin-3表达的siRNA1及siRNA2),使用MTT法分别检测转染12、24及48 h细胞增殖率;使用Western-blot方法检测转染48 h后各组Galectin-3表达情况及对bcl-2、survivin、MMP-9表达的影响;Transwell小室检测细胞侵袭能力.结果:沉默Galectin-3后人甲状腺乳头状癌细胞的Galectin-3表达与空白对照组及阴性对照组相比明显降低(P<0.05),差异有统计学意义;细胞增殖、侵袭能力明显下降(P<0.05),差异有统计学意义;Bcl-2、Survivin、MMP-9蛋白表达显著低于空白对照组及阴性对照组(P<0.05),差异有统计学意义.结论:沉默Galectin-3基因表达可以降低人甲状腺乳头状癌细胞增殖及侵袭能力,该机制可能与其降低bcl-2、Survivin及MMP-9蛋白表达有一定相关性.     

胸腔镜下手汗症手术方法的改进

目的 探讨改进后胸腔镜下手汗症手术方法的临床效果.方法 采用改进胸腔镜下胸交感神经切断术治疗105例手汗症患者,电刀单纯切断第3胸交感神经.结果 105例患者术后手汗均消失,腋窝汗明显减少,术后气胸5例.无出血和Horner's综合征等严重并发症.术后随访1~12个月,15例出现代偿性多汗.结论 改进的手术方法具有操作简单、安全有效、创伤小、并发症少等特点,值得临床推广.     

复方丹参滴丸对胰岛素抵抗大鼠的保护作用及其机制研究

目的观察复方丹参滴丸对胰岛素抵抗大鼠糖脂代谢的作用,探讨其改善胰岛素抵抗的机制。方法隔日1次肌肉注射地塞米松1mg/kg诱导SD大鼠产生胰岛素抵抗,并随机分为正常对照组、模型对照组、吡格列酮组、丹参注射针组及复方丹参滴丸浸膏组。给药8周后分别检测空腹血糖、空腹胰岛素、一氧化氮合酶、一氧化氮、胸主动脉肌层/管壁厚度,并采用ELISA法测定脂联素浓度及实时PCR法测定大鼠骨骼肌细胞膜葡萄糖转运蛋白-4 mRNA表达。结果造模成功大鼠胰岛素抵抗指数增加,一氧化氮合酶生成减少,一氧化氮降低,胸主动脉肌层/管壁厚度明显增加,脂联素分泌减少,肌细胞膜葡萄糖转运蛋白-4 mRNA表达降低。而经上述3种药物干预后,大鼠的胰岛素抵抗指数均有不同程度降低,一氧化氮合酶及一氧化氮生成增加,胸主动脉肌层/管壁厚度明显降低,脂联素分泌及肌细胞膜葡萄糖转运蛋白-4 mRNA表达增强（P〈0.05或P〈0.01）,且吡格列酮组及复方丹参滴丸浸膏两组间作用无统计学意义（P〉0.05）;丹参注射针组作用较弱（P〈0.05）。结论复方丹参滴丸通过多靶点调节糖脂代谢而发挥改善胰岛素抵抗的作用,并与其促进脂联素分泌、增强骨骼肌细胞膜葡萄糖转运蛋白-4 mRNA表达密切相关。     

Serum calcitonin negative mixed medullary‐follicular carcinoma initially diagnosed as medullary thyroid carcinoma by fine‐needle aspiration cytology: A case report and review of the literatures

Medullary thyroid carcinoma (MTC) is potentially lethal. A prompt and accurate diagnosis is the prerequisite for the treatment of MTC. Fine‐needle aspiration (FNA) is a reliable diagnostic tool in the assessment of thyroid nodules. However, cytologic assessment of MTC based on FNA has several drawbacks due to morphological variants. We present a case of MTC diagnosed through FNA cytology, which was eventually histologically confirmed as a mixed medullary‐follicular carcinoma with negative serum calcitonin expression. Hence, diagnosis of MTC based on FNA should be applied with caution. Ultrasound characteristics of suspicious thyroid nodules are recommended to be evaluated by FNA. However, calcitonin levels should be measured in both the FNA washout fluid and serum when features of MTC are presented or cytology result is inconclusive. If adequate FNA sample is available, a supplementary immunocytochemical staining of markers such as calcitonin, chromogranin, carcinoembryonic antigen, and thyroglobulin is helpful for a correct diagnosis of MTC.     

Protective effect of paeonol on beta-amyloid 25-35- induced toxicity in PC12 cells

BACKGROUND: Paeonol is a primary phenolic component of the Chinese medicinal herb Cortex moutan. Recent studies have shown that paeonol has anti-inflammatory, analgesic, and antioxidative effects as well as a significant cardioprotective effect against myocardial ischemia.OBJECTIVE: To investigate the protective effect of paeonol on β-amyloid 25-35-induced toxicity in PC12 cells and analyze its mechanism of action.DESIGN, TIME AND SETTING: A controlled repeated-measures cell-based study was performed in the Department of Pharmacology of Guangdong Medical College between September 2006 and December 2007.MATERIALS: Paeonol was supplied by Xuancheng Baicao Plant Industry and Trade Company, China.PC12 cells were a kind girl from Dr. Haitao Zhang at Guangdong Medical College. Β-amyloid 25-35was purchased from Sigma Company, USA. Lactate dehydrogenase (LDH) and malondialdehyde (MDA)kits were purchased from Nanjing Jiancheng Bioengineering Research Institute, China.METHODS: PC12 cells were maintained in Dulbecco's modified eagle's medium (DMEM) supplemented with 100 mL/L heat-inactivated horse serum and 50 mL/L fetal bovine serum at 37℃ and cultured in an incubator with 5% CO2. The medium was renewed every other day. Batches of cells were assigned into three groups. (1) Paeonol group: cells were preincubated with different concentrations of paeonol (12, 25or 50 μ mol/L) for one hour and β-amyloid 25-35 was added to the medium; (2) control group: cells were cultured in DMEM supplemented with 100 mL/L heat-inactivated horse serum and 50 mL/L fetal bovine serum; and (3) β-amyloid 25-35 group: β-amyloid 25-35 was added to the medium.MAIN OUTCOME MEASURES: When PC12 cells in each group were cultured for 24 hours, the cell viability was determined using the MTT reduction assay, LDH release into the culture media was measured by 2,4-dinitrophenylhydrazine chromatometry and MDA content was measured using a thiobarbituric acid assay.RESULTS: When PC12 cells were treated with β -amyloid 25-35 (50 μ mol/L) for 24 hours, their viability was significantly lower compared with the control group (P < 0.01). When the cells were treated with paeonol for one hour prior to incubation with β -amyloid 25-35, their viability was significantly increased compared with the β -amyloid 25-35 group (P < 0.05-0.01), LDH activity and MDA level in the β -amyloid 25-35 group were significantly increased compared with the control group (P < 0.01). When the cells were treated with different concentrations of paeonot, LDH activity and MDA level in PC12 cells were significantly decreased compared with the β-amyloid 25-35 group (P < 0.01).CONCLUSION: Paeonol protects PC12 cells against β-amyloid 25-35-induced toxicity and the protective effect of paeonol is probably achieved through its antioxidative effects.     

巨大左心室患者心脏瓣膜手术的围术期特点及其处理

目的：探讨巨大左心室患者心脏瓣膜手术围术期的病情及其处理特点。方法：回顾性分析１９９０年１月至１９９８年１２月１２９例巨大左心室（收缩期末直径５．０４￣８．１４ｃｍ）患者心脏前膜手术的临床资料,其中行二尖瓣置换或成形术（ＭＶＲＰ）３１例、主动脉瓣置换术（ＡＶＲ）３４例、二尖瓣与主动脉瓣双瓣置换术（ＤＶＲ）６４例。结果：患者以中青年男性为主（８３．７２％）;瓣膜病变以关闭不全为主;病因主要为风湿性（     

苯那普利和氨氯地平对高血压病患者蛋白尿及肾功能的作用

目的：观察比较苯那普利和氨氯地平对高血压患者白蛋白尿及肾功能的疗效。方法：３０例未曾治疗的高血压病患者随机分为分两组,Ｉ组患者用苯那普利每日１０ｍｇ治疗１２周,后用氨氯地平每日１０ｍｇ治疗１２周,ＩＩ组患者先用氨氯地平每日１０ｍｇ治疗１２周,后用苯那普利每日１０ｍｇ治疗１２周,结果：两类药物能同等程度地降低患者的动脉血压,两组患者在苯那普利的期间白蛋白尿均显著减少（Ｐ〈０．０１）,而氨氯地平对白蛋     

mTOR结合蛋白的研究进展

mTOR(mammalian target of rapamycin,mTOR)是雷帕霉素在哺乳动物细胞内作用的蛋白激酶,通过PI3K/Akt信号磷酸化激活而调控细胞分裂、促进转录、信号翻译等。研究表明,mTOR在肿瘤的形成和发展中扮演着非常重要的角色,并参与肿瘤的侵袭和转移。目前,以mTOR为治疗靶点成为肿瘤治疗的研究新热点。本文就近年来有关mTOR靶标及其结合蛋白研究进展作一综述。     

阿托伐他汀与低分子量肝素联用对急性冠状动脉综合征早期复发性缺血事件的预防作用

将149例急性冠状动脉综合征（ACS）患者随机分为常规治疗组、克赛（低分子量肝素）组和克赛立普妥（阿托伐他汀）联合治疗组，观察治疗后三组心绞痛平均发作次数、心绞痛平均持续时间、心脏事件发生病例数。结果：治疗后三组心绞痛平均发作次数、心绞痛平均持续时间均比治疗前明显减少（P＜0．001）；与常规治疗组比较，克赛组、联合治疗组治疗第2周和第4周心绞痛发作次数及心脏事件发生病例数均显著降低（P＜0．01）；联合治疗组治疗后第4周心绞痛发作例数低于克赛组（P＜0．05）。提示联合应用阿托伐他汀与低分子量肝素可显著降低ACS早期复发性缺血事件的发生。