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61.
输血作为一种重要的医疗救治手段,已被广泛地应用于临床,输血的不良反应已引起医护人员的高度重视,并制定了安全的输血制度和规范的输血技术操作常规.但在输血的不良反应中未见有静脉输注血小板后患者出现双下肢疼痛的报道.我科一例ABO血型不合异基因造血干细胞移植"零期"的患者输注血小板后出现双下肢疼痛,经过与医生探讨和总结临床经验,对患者进行护理干预,取得满意效果,现报道如下. 相似文献
62.
目的探讨双水平气道正压通气联合纳洛酮治疗Ⅱ型呼吸衰竭。方法采用经鼻罩/鼻面罩BiPAP呼吸机辅助通气,联合纳洛酮治疗16例Ⅱ型呼吸衰竭患者。结果13例患者血气指标明显改善,预后良好。结论双水平气道正压通气联合纳洛酮是治疗Ⅱ型呼吸衰竭的一种有效手段,且因并发症少、疗程短、费用和死亡率低等优点,值得临床推广。 相似文献
63.
64.
BACKGROUND: It has been previously shown that hyperbaric oxygen may promote proliferation of neural stem cells and reduce death of endogenous neural stem cells (NSCs).
OBJECTIVE: To explore the effects of hyperbaric oxygen on the differentiation of hypoxic/ischemic brain-derived NSCs into neuron-like cells and compare with high-concentration oxygen and high pressure.
DESIGN, TIME AND SETTING: An in vitro contrast study, performed at Laboratory of Neurology, Central South University between January and May 2006.
MATERIALS: A hyperbaric oxygen chamber (YLC 0.5/1A) was provided by Wuhan Shipping Design Research Institute; mouse anti-rat microtubule-associated protein 2 monoclonal antibody by Jingmei Company, Beijing; mouse anti-rat glial fibrillary acidic protein monoclonal antibody by Neo Markers, USA; mouse anti-rat galactocerebroside monoclonal antibody by Santa Cruz Biotechnology Inc., USA; and goat anti-mouse fluorescein isothiocyanate-labeled secondary antibody by Wuhan Boster Bioengineering Co., Ltd., China.
METHODS: Brain-derived NSCs isolated from brain tissues of neonatal Sprague Dawley rats were cloned and passaged, and assigned into five groups: normal control, model, high-concentration oxygen, high pressure, and hyperbaric oxygen groups. Cells in the four groups, excluding the normal control group, were incubated in serum-containing DMEM/F12 culture medium. Hypoxic/ischemic models of NSCs were established in an incubator comprising 93% N2, 5% 002, and 2% 02. Thereafter, cells were continuously cultured as follows: compressed air (0.2 MPa, 1 hour, once a day) in the high pressure group, compressed air + a minimum of 80% 02 in the hyperbaric oxygen group, and a minimum of 80% Q2 in the high-concentration oxygen group. Cells in the normal control and model groups were cultured as normal.
MAIN OUTCOME MEASURES: At day 7 after culture, glial fibrillary acidic protein, microtubule-associated protein 2, and galactocerebroside immunofluorescence staining were examined to observe differentiation and calculate the percentage of NSCs differentiating into neuron-like cells or neuroglia-like cells.
RESULTS: Neuron-like cells or neuroglia-like cells were visualized in all five groups. There were no significant differences in the percentage of differentiating cells between the hyperbaric oxygen group and the normal control group (P 〉 0.05). The percentage of NSCs differentiating into neuron-like cells in the hyperbaric oxygen group was significantly greater than model, high-concentration oxygen, and high pressure groups; however, the percentage differentiating into neuroglia-like cells was significantly lower (P 〈 0.01 ).
CONCLUSION: Hyperbaric oxygen promotes the differentiation of brain-derived neural stem cells into neuron-like cells but inhibits differentiation into neuroglia-like cells. Furthermore, the efficacy of hyperbaric oxygen is superior to high-concentration oxygen and high pressure. 相似文献
OBJECTIVE: To explore the effects of hyperbaric oxygen on the differentiation of hypoxic/ischemic brain-derived NSCs into neuron-like cells and compare with high-concentration oxygen and high pressure.
DESIGN, TIME AND SETTING: An in vitro contrast study, performed at Laboratory of Neurology, Central South University between January and May 2006.
MATERIALS: A hyperbaric oxygen chamber (YLC 0.5/1A) was provided by Wuhan Shipping Design Research Institute; mouse anti-rat microtubule-associated protein 2 monoclonal antibody by Jingmei Company, Beijing; mouse anti-rat glial fibrillary acidic protein monoclonal antibody by Neo Markers, USA; mouse anti-rat galactocerebroside monoclonal antibody by Santa Cruz Biotechnology Inc., USA; and goat anti-mouse fluorescein isothiocyanate-labeled secondary antibody by Wuhan Boster Bioengineering Co., Ltd., China.
METHODS: Brain-derived NSCs isolated from brain tissues of neonatal Sprague Dawley rats were cloned and passaged, and assigned into five groups: normal control, model, high-concentration oxygen, high pressure, and hyperbaric oxygen groups. Cells in the four groups, excluding the normal control group, were incubated in serum-containing DMEM/F12 culture medium. Hypoxic/ischemic models of NSCs were established in an incubator comprising 93% N2, 5% 002, and 2% 02. Thereafter, cells were continuously cultured as follows: compressed air (0.2 MPa, 1 hour, once a day) in the high pressure group, compressed air + a minimum of 80% 02 in the hyperbaric oxygen group, and a minimum of 80% Q2 in the high-concentration oxygen group. Cells in the normal control and model groups were cultured as normal.
MAIN OUTCOME MEASURES: At day 7 after culture, glial fibrillary acidic protein, microtubule-associated protein 2, and galactocerebroside immunofluorescence staining were examined to observe differentiation and calculate the percentage of NSCs differentiating into neuron-like cells or neuroglia-like cells.
RESULTS: Neuron-like cells or neuroglia-like cells were visualized in all five groups. There were no significant differences in the percentage of differentiating cells between the hyperbaric oxygen group and the normal control group (P 〉 0.05). The percentage of NSCs differentiating into neuron-like cells in the hyperbaric oxygen group was significantly greater than model, high-concentration oxygen, and high pressure groups; however, the percentage differentiating into neuroglia-like cells was significantly lower (P 〈 0.01 ).
CONCLUSION: Hyperbaric oxygen promotes the differentiation of brain-derived neural stem cells into neuron-like cells but inhibits differentiation into neuroglia-like cells. Furthermore, the efficacy of hyperbaric oxygen is superior to high-concentration oxygen and high pressure. 相似文献
65.
目的从蛋白质组学角度探讨偏头痛肝阳上亢证证候相关蛋白及快速埋线缓解疼痛和改善肝阳上亢证症状的机制。方法用固相pH梯度双向凝胶电泳分离正常大鼠及肝阳上亢证大鼠经快速埋线治疗前、后肾上腺的总蛋白质,经考马斯亮兰染色,PDQuest 7.0软件分析。结果与正常组匹配,肝阳上亢证组与快速埋线组大鼠肾上腺图象匹配率分别为85%、89%,且重复性较好,其蛋白质主要分布在等电点PI3-8,分子量M r14.4-75kD范围。与正常组比较,肝阳上亢证组中蛋白质点表达上升2倍及以上的点有10个,下降2倍及以上的点有12个。而在快速埋线组中这些表达上升的点均有下降,有11个点下降达2倍以上,其中有7个下降后接近于正常组;而表达下降的点均有上升,有8个点上升达2倍以上,其中有5个点上升后接近于正常组。结论这些有显著差异的蛋白质可能是偏头痛肝阳上亢证证候相关蛋白;快速埋线缓解头痛和改善临床症状也可能与上述蛋白质的改变有关。 相似文献
66.
目的研究高压氧(HBO)对神经精神性狼疮(NPSLE)患者SLE疾病活动性指数(SLEDAI)的影响及临床疗效。 方法将60例NPSLE患者随机分为HBO组和对照组,对照组给予常规药物治疗及对症处理,HBO组则加用HBO干预。分别测定2组患者治疗前、后SLEDAI积分,并对2组患者临床疗效进行比较。 结果2组患者分别经3个疗程治疗后,发现HBO组有效率为80.0%,对照组有效率为53.3%,HBO组有效率明显高于对照组(P<0.05)。2组患者治疗前,其SLEDAI积分均明显偏高,治疗后2组患者SLEDAI积分均显著降低(P<0.05),且HBO组患者治疗后SLEDAI积分明显低于对照组水平(P<0.05)。 结论HBO能进一步降低NPSLE患者疾病活动度,抑制机体免疫功能,促进患者康复。 相似文献
67.
目的:探讨快速埋线缓解肝阳上亢型偏头痛大鼠与相关蛋白的机理。方法:30只大鼠随机抽取20只制成肝阳上亢证模型后又复制为实验性偏头痛模型,其中10只给予快速埋线治疗(埋线组);10只给予蒸馏水灌服(上亢组);另10只为正常组。用双向凝胶电泳检测3组大鼠下丘脑的总蛋白质的表达。结果:与正常组匹配,上亢组与埋线组大鼠下丘脑图象匹配率分别为92%、96%,且重复性较好,其蛋白质主要分布在等电点PI4-8,分子量Mr20-75 kD。与正常组比较,上亢组蛋白质点表达上升2倍及以上12个,下降2倍及以上14个;埋线组以上表达上升的点有13个下降2倍以上,其中8个点下降后接近正常组,而表达下降的点均上升,有9个点上升达2倍以上,其中6个点上升后接近于正常组。结论:与正常组差异有显著的蛋白质可能是偏头痛肝阳上亢证证候相关蛋白;快速埋线可缓解头痛和改善临床症状可能与上述蛋白质的改变有关。 相似文献
68.
针刺对偏头痛大鼠血浆神经肽的影响 总被引:5,自引:1,他引:5
目的:探讨针刺治疗偏头痛对降钙素基因相关肽(calatonin gene-ralated peptide,CGRP)、P物质、内皮素、神经肽Y、心钠素、神经降压肽(neurotensin,NT)的影响。方法:将40只SD大鼠数字表法随机分为正常对照组、模型对照组、针刺对照组和针刺治疗组。按各组方案造模,或针刺治疗后,采用放射免疫法测定颈静脉血浆中多种神经肽的含量。结果:模型对照组CGRP(319.64&;#177;53.12)ng/L;P物质(126.76&;#177;39.29)ng/L;心钠素(366.11&;#177;83.12)ng/L;内皮素(785.00&;#177;186.64)ng/L;神经肽Y(4567.064&;#177;1041.86)ng/L均较正常对照组(152.87&;#177;31.82),(57.43&;#177;21.03),(278.31&;#177;58.76),(516.88&;#177;126.89),(3232.75&;#177;424.87)ng/L有明显升高(t=2.283—2.791,P&;lt;0.05或0.01),而模型对照组NT(56.64&;#177;21.21)ng/L较正常对照组(113.12&;#177;35.92)ng/L明显降低(t=2.842,P&;lt;0.01);针刺治疗组CGRP(148.54&;#177;19.18)ng/L;P物质(60.76&;#177;22.31)ng/L;心钠素(287.63&;#177;68.64)ng/L;内皮素(524.38&;#177;122.55)ng/L;神经肽Y(3324.56&;#177;576.77)ng/L均明显下降(t=2.833—2.481,P&;lt;0,05或P&;lt;0.01),NT(101.13&;#177;27.69)ng/L较模型对照组明显升高(t=2.669,P&;lt;0.05)。结论:针刺调节脑神经肽的合成与释放,是其治疗偏头痛的主要因素之一。 相似文献
69.
目的探讨希望水平在晚期肺癌患者抑郁与心理韧性水平的中介效应,为临床医护人员开展心理韧性相关干预,改善患者抑郁状态提供理论依据。方法采用便利抽样法选取2019年10月至2020年12月在北京协和医院肺癌诊治中心接受规律治疗的202例肺癌患者为研究对象。应用Herth希望量表、心理韧性量表(CD-RISC)、抑郁自评量表(SDS)以及自行设计的患者人口学特征和疾病特征资料调查表对患者进行问卷调查。结果晚期肺癌患者抑郁水平与患者心理韧性呈负相关(r=-0.50,P<0.01),患者希望水平与心理韧性呈正相关(r=0.62,P<0.01),患者抑郁水平与希望水平呈负相关(r=-0.44,P<0.01);晚期肺癌患者希望水平在抑郁状态与心理韧性中存在部分中介效应,中介效应值为-16.51(95%CI=-0.20~-0.03),中介效应占比为19.83%。结论接受规律治疗的晚期肺癌患者抑郁程度较高,希望在抑郁状态和心理韧性之间起到部分中介效应。医护人员可通过干预提升晚期肺癌患者的信心和希望,改善悲观情绪。 相似文献
70.
目的:通过观察服用天麻钩藤饮后高血压肝阳上亢证大鼠下丘脑的蛋白质的改变,从蛋白质组学角度探讨天麻钩藤饮的降血压和改善肝阳上亢证症状的机制。方法:实验于2005-10/2006-06在中南大学湘雅医院完成。①动物与中药:自发性高血压大鼠20只,完全随机(随机排列表法)分为治疗组及对照组各10只;正常SD大鼠10只(正常组)。附子汤:由60g单味制附子组成,先将药物浸泡30min,煎煮两次后再将药液合并浓缩为含生药0.1g/mL。天麻钩藤饮:天麻15g,钩藤15g,石决明20g,黄芩10g,杜仲10g,牛膝10g。石决明先煎20min,天麻后下;煎煮两次后再将药液合并浓缩为含生药0.1g/mL。②高血压肝阳上亢证模型的复制:所有动物同时喂养,治疗组及对照组连续灌服附子汤共21d,复制成高血压肝阳上亢证模型(收缩压明显增高,饮水量增加,性情变得更易激怒为造模成功)。③对照组自第22天起改为连续灌服蒸馏水14d,治疗组自第22天起改为连续灌服天麻钩藤饮14d,均于第36天处死。正常组大鼠,连续灌服蒸馏水35d,于第36天处死。④用固相pH梯度双向凝胶电泳分离各组大鼠下丘脑的总蛋白质,经考马斯亮兰染色,PDQuest7.0软件分析。结果:30只大鼠均进入结果分析。①治疗后治疗组大鼠的血压明显下降,饮水量减少,性情变得温和。②大鼠的下丘脑蛋白质图像匹配率达94%~100%,且重复性较好,其蛋白质主要分布在等电点4~8,相对分子质量14400~75000。治疗后有67个蛋白质点表达上升2倍及以上,有19个蛋白质点表达下降2倍及以上。结论:天麻钩藤饮治疗后高血压肝阳上亢证大鼠血压降低、症状改善,这可能与其下丘脑蛋白质的改变有关。 相似文献