3080张西药处方的调查与分析

处方在医师、药剂调配者、患者三者之间起着非常重要的桥梁作用。开好一张处方 ,它不仅反映了医药人员的业务素质和责任心 ,还直接关系着用药的效果。本人调查了 1999年 10月份我科全部门诊西药处方 ,并对处方的书写状况与一些不合理用药现象作了分析探讨。1　材料与方法　　从药房查阅 1999年 10月全部门诊西药处方 ,并作了如下数据处理 :统计全月西药处方总数和书写不符合要求的处方数 ;不合理用药的处方数。2　结　果2 .1　被查的全月西药处方 3 0 80张 ,书写不符合要求者 93 2张 ,占调查总数的 3 0 .2 6% ,其中前记不全 10张 ,占2 8.12 …     

丹参在急性肝损伤时对枯否细胞功能的调整作用

目的探讨丹参在急性肝损伤时对枯否细胞功能的调整作用。方法取大鼠40只,随机分成4组,正常对照组(对照组),硫代乙酰胺组(TAA 组),三氯化钆加硫代乙酰胺组(GdCl_3加 TAA 组)和三氯化钆加硫代乙酰胺加丹参组(丹参组),后两组予大鼠鼠尾静脉注射 GdCl_3共2日。丹参组给大鼠皮下注射丹参醇提取液,除正常对照组,各组均用 TAA 给大鼠灌胃造成大鼠急性肝损伤。观察细胞巨噬指数、肿瘤坏死因子(TNF-α)、谷丙转氨酶(ALT)、内毒素及总胆红素(TB)等指标。结果丹参组吞噬指数较 GdCl_3加 TAA 及 TAA组明显增高(P<0.05);而血浆内毒素、TNF-α、ALT 及 TB 水平较 GdCl_3加 TAA 及 TAA 组明显下降(P<0.05)。结论丹参具有增强封闭状态枯否细胞吞噬功能并使炎性介质 TNF-α分泌减少,减轻肝细胞损伤。     

高脂血症性胰腺炎

自1952年Klaskin等[1]报告1例原发性高脂血症导致胰腺炎反复发作后,现已明确地认识到高脂血症是胰腺炎的病因之一。鉴于其发生与血清胆固醇升高无关,而与血清三酰甘油(TG)水平显著升高密切相关,故高脂血症性胰腺炎又称为“高三酰甘油血症性胰腺炎”(hypertriglyceridaemia pancreatitis,HLP)。临床上通常以TG>11.30mmol/L(1000mg/dl)或血TG在5.65～11.3mmol/L且出现乳糜样血清即可诊断为HLP。12%～38%的AP患者伴有血脂的异常升高,在某些家族性高脂血症患者和妊娠高脂血症患者中常反复发生AP[2,3]。一、高脂血症的病因根据脂蛋白电泳…     

脂肪性肝病与胃食管反流病的相关性研究

目的评估脂肪性肝病(FLD)与胃食管反流病GERD之间的关系。方法选取2 000例受试者,采用较简便的胃食管反流病问卷(GerdQ)作为初筛GERD诊断标准,对入选者均进行腹部超声波等检查,分析FLD与GERD关系。结果对2 000例患者利用GerdQ评估GERD诊断情况,有176例(8.8%)诊断为GERD,女68人(38.64%),男108人(61.36%);脂肪肝患者760例,患病率38.0%,在脂肪肝组中,男女性GERD症状的GerdQ阳性率分别为13.77%和12.24%,两者无明显差异(P>0.05)。脂肪肝人群发生GERD症状的患病率与对照组存在统计学差异(P<0.05)。结论脂肪肝与GERD显著相关,控制脂肪肝发展可以降低GERD发病率。     

基质细胞衍生因子/趋化因子受体4轴参与胰腺癌细胞侵袭的体外实验研究

Objective To investigate the expression of chemokine receptor (CXCR)4 in human pancreatic cancer cell lines,and its association with proliferation,adhesion and invasion of pancreatic cancer cells.Methods The CXCR4 mRNA and protein in three pancreatic cancer cell lines were detected by RT-PCR and Western blotting,respectively.Confocal microscopy was used to detect the fluorescence intensity induced by SDF-lα in AsPC-1 cells.MTT test was performed to study the proliferation of pancreatic cancer cells.The invasive ability of pancreatic cell lines was determined by transwell invasion assay kit and the adhesive ability was detected by cell adhesive test in vitro.Results There were expressions of CXCR4 mRNA and protein in different extent in three pancreatic cancer cell lines.The strong expression was seen in AsPC-1 cell line,but weak expression in SW1990 cell line.The CXCR4 was functional expressed on AsPC-1 ceils.SDF-1α improved the proliferation,adhesion and invasion of three pancreatic cancer cell lines,especially in AsPC-1 cell line,while the proliferation in SW1990 cell line was weak.But all above effects of the SDF-1α could be inhibited by AMD3100.Conclasions CXCR4 mRNA and protein were expressed in pancreatic cancer cell lines.The efficacy that SDF-1 can increase the invasive ability of pancreatic cancer ceils through SDF-1/CXCR4 axis is closely related to the expression of CXCR4.     

基质细胞衍生因子/趋化因子受体4轴参与胰腺癌细胞侵袭的体外实验研究

Objective To investigate the expression of chemokine receptor (CXCR)4 in human pancreatic cancer cell lines,and its association with proliferation,adhesion and invasion of pancreatic cancer cells.Methods The CXCR4 mRNA and protein in three pancreatic cancer cell lines were detected by RT-PCR and Western blotting,respectively.Confocal microscopy was used to detect the fluorescence intensity induced by SDF-lα in AsPC-1 cells.MTT test was performed to study the proliferation of pancreatic cancer cells.The invasive ability of pancreatic cell lines was determined by transwell invasion assay kit and the adhesive ability was detected by cell adhesive test in vitro.Results There were expressions of CXCR4 mRNA and protein in different extent in three pancreatic cancer cell lines.The strong expression was seen in AsPC-1 cell line,but weak expression in SW1990 cell line.The CXCR4 was functional expressed on AsPC-1 ceils.SDF-1α improved the proliferation,adhesion and invasion of three pancreatic cancer cell lines,especially in AsPC-1 cell line,while the proliferation in SW1990 cell line was weak.But all above effects of the SDF-1α could be inhibited by AMD3100.Conclasions CXCR4 mRNA and protein were expressed in pancreatic cancer cell lines.The efficacy that SDF-1 can increase the invasive ability of pancreatic cancer ceils through SDF-1/CXCR4 axis is closely related to the expression of CXCR4.     

一起水源性污染引起的细菌性食物中毒调查报告

2006年8月25日-9月3日，大理州宾川县鸡足山镇炼洞村委会北卡拉村一户村民办理 “满月酒”，发生水源性污染导致群体性食物中毒，共发病213人。事件发生后，县委、县人民政府及时启动突发公共卫生事件应急处置预案，开展疫情调查处理和救治工作。经采取相应措施后，疫情得到了有效控制，现将调查结果报告如下。     

不同pH值下苦参总碱透皮速率的研究

通过对苦参总碱在不同ＰＨ值下的透皮研究,考察ＰＨ值为影响药物透皮速率的重要因素。实验结果表明苦参碱的溶解度随ＰＨ值的升降低,稳态透皮速率随着ＰＨ值的变化而变化。在ＰＨ值为９．１时,透皮速率最大。根据分子型药物一离子型药物透过皮肤的途径不同,讨论了不同途径对皮肤渗透的影响。     

肺癌100例诊断与治疗分析

为使肺癌患者能够得到早期诊断和治疗，将１９８９年至１９９５年内科住院１００例肺癌患者进行年龄、吸烟、临床表现、Ｘ线特征、诊断方法和治疗措施进行分析比较，Ｘ线检查特征发现肺癌高发年龄在４０岁～７０岁（占６０％），１０％的患者小于４０岁，有趋于年轻化趋势，有６２％患者有吸烟史，吸烟与肺癌发生有关，临床表现无特异性，全身表现多样化，多种诊断方法有利于提高肺癌确诊率纤支镜法５７％，脱落细胞学（胸水、痰）２２％，活组织检查（淋巴结、胸膜）１７％，早期诊断困难，晚期肺癌应用以化疗或放疗为主的综合治疗，有利于提高患者的生存率和改变生活质量，全身化学治疗的总有效率５６．７％，局部化学治疗的总有效率６３．６％。