Studies on Toxicity of Nitroquine-Dapsone Compound and Therapeutic Effects of Folic or Folinic Acid on Toxicated Animals

The toxic effects of nitroquine-dapsone compound(NQD)in mice and dogs were studied.The therapeutic index of NQDin mice is 1911,the greatest among the 6 antimalarials tested.Thetoxic effects of NQD(50 mg/kg/day for 3 days per os)and nitro-quine in dogs were manifested by injuries on the adrenal cortexand intestinal epithelium.When folic acid(4 mg/kg/day for 4 days)or calcium leucovorinum(0.3 mg/kg/day for 4 days)were usedconcomitantly with NQD,the death rate and the incidence of dia-rrhea in the toxicated dogs were greatly reduced,the injury on theintestinal epithelium was much milder,and the goblet cells weremuch more numerous than those without treatment.The results suggestthat folic acid and calcium leucovorinum can protect the undifferen-tiated cells in the intestinal crypts from being injured by NQD.     

针刀配合膝关节间断主动活动治疗膝关节强直

膝关节强直是膝关节及关节周围创伤、关节炎晚期及手术后的严重并发症,同时伴有髌骨活动度严重减少甚至消失.病理多为髌上囊及两侧沟的广泛粘连,部分患者伴有关节外结构的挛缩.物理治疗几乎无效、开放性手术切开松解及关节镜下松解创伤大,出血多,存在再粘连的可能性,手术并发症多,一次手术失败,没有再次松解的机会.我院自1997年8月-2002年3月,应用针刀闭合松解加关节间断主动活动治疗严重膝关节粘连52例疗效满意.……     

5名电离辐射事故患者外周血T细胞T细胞抗原受体、T细胞分化抗原决定簇－3效应的研究

文中报道了５名事故性急性骨髓型放射病患者照后２．５和３．５年外周血Ｔ淋巴细胞Ｔ细胞受体（ＴＣＲ）基因，ＴＣＲ、Ｔ细胞分化抗原决定簇－３（ＣＤ_３）表达与ＴＣＲ／ＣＤ_３复合物功能的辐射效应．发现５名患者于照后２．５年，２名（５．２Ｇｙ和２．４Ｇｙ，５５岁）于照后３．５年外周血Ｔ细胞应答抗ＣＤ３单抗刺激而增殖的能力尚未完全恢复；经同时用ＩＬ－２和抗ＣＤ_３单抗刺激，增殖能力比单用抗ＣＤ_３单抗刺激有所增强；后２名的外周血ＴＣＲ、ＣＤ_３阳性细胞百分率一直低于正常对照和其他患者；并见一患者出现ＤＮＡ重排杂交带型．本文并从ＴＣＲ／ＣＤ_３在介导Ｔ细胞抗原刺激反应中的作用，电离辐射对ＴＣＲ／ＣＤ_３复合物的影响，后果和意义等方面进行了讨论．     

Presentation of viral antigens restricted by H-2Kb,Db or Kd in proteasome subunit LMP2-and LMP7-deficient cells

In the class II region of the major histocompatibility complex (MHC), four genes implicated in MHC class I-mediated antigen processing have been described. Two genes (TAP 1 and TAP 2) code for multimembrane-spanning ATP-binding transporter proteins and two genes (LMP 2 and LMP 7) code for subunits of the proteasome. While TAP 1 and TAP 2 have been shown to transport antigenic peptides from the cytosol into the endoplasmic reticulum, where the peptides associate with MHC class I molecules, the role of LMP 2/7 in antigen presentation is less clear. Using antigen processing mutant T2 cells that lack TAP 1/2 and LMP 2/7 genes, it was recently shown that expression of TAP 1/2 alone was sufficient for processing and presentation of the influenza matrix protein M1 as well as the minor histocompatibility antigen HA-2 by HLA-A2. To understand if presentation of a broader range of viral antigens occurs in the absence of LMP 2/7, we transfected T2 cells with TAP 1, TAP 2 and either of the H-2K^b, D^b or K^d genes and tested their ability to present vesicular stomatitis vires and influenza virus antigens to virus-specific cytotoxic T lymphocytes. We found that T2 cells, expressing TAP 1/2 gene products, presented all tested viral antigens restricted through either the H-2K^b, D^b or K^d class I molecules. We conclude that the proteasome subunits LMP 2/7 as well as other gene products in the MHC class II region, except from TAP 1/2, are not generally necessary for presentation of a broader panel of viral antigens to cytotoxic T cells. However, the present results do not exclude that LMP 2/7 in a more subtle way may, or in rare cases completely, affect processing of antigen for presentation by MHC class I molecules.     

冠心病家族史大学生apo E基因多态性的临床研究

目的:探讨大学生载脂蛋白E(apoE)基因多态性,及其对血脂代谢紊乱、动脉粥样硬化(AS)和冠心病(CHD)的影响。方法:对广东地区高校152名健康汉族大学生,在问卷调查的基础上,根据一、二级亲属有无CHD病史分为阳性组与对照组,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法进行apoE基因分析。结果:有CHD家族史的阳性组e_4等位基因频率显著高于无CHD家族史的阴性组(P<0.05)。结论:apoE基因多态性与AS、CHD易感性密切有关,e_4可能是CHD的重要遗传因子。     

Combination therapy with afatinib and bevacizumab in an EGFR-mutated non-small cell lung cancer patient with acquired ERBB2 amplification: A case report

Introduction:Acquired resistance to reversible EGFR tyrosine kinase inhibitors remains a significant obstacle, and acquired ERBB2 amplification is the most common “bypass” mechanism. For patients with sensitizing EGFR mutation who experience resistance via ERBB2 amplification, no targeted drug has been demonstrated to be effective.Patient concerns:A 56-year-old female nonsmoker suffered from left leg paralysis and low back pain. Imaging examination revealed a mass in the anterior segment of the right upper lobe lung and possible multiple metastases in the right hilar, mediastinal lymph nodes, bone metastases, and soft tissue invasion.Diagnosis:Transbronchial lung biopsy revealed a moderately differentiated adenocarcinoma (cT4N2M1c, stage IV). An EGFR exon 19 deletion was identified using amplification refractory mutation system.Interventions:After the patient was treated with gefitinib initiation (250 mg/d) for 15 months, the tumor progressed with ERBB2 amplification revealed by next-generation sequencing test. Then, the patient was started on afatinib (40 mg/d) plus bevacizumab (7.5 mg/kg every 3 weeks).Outcomes:The combination therapy of afatinib and bevacizumab in this patient was effective with some slight side effects. Computed tomography scans showed the tumor shrinkage and the pleural effusion disappeared in the right lung. The overall survival was 23.5 months.Conclusion:To date, there is no targeted therapy approved and demonstrated to be effective for non-small cell lung cancer patients with EGFR sensitizing mutations, and ERBB2 amplification. The effectiveness of combination therapy with afatinib and bevacizumab may provide a new therapeutic option for these patients.     

正畸支抗之“惑”

与医学其他学科一样,口腔正畸学也不乏用模糊概念来取代精确测量的习惯做法,正畸支抗控制便是其中之一。随着循证医学的发展,正畸医师越来越重视临床证据的准确性,于是,以往对于正畸支抗控制的经验性描述逐渐显露出其弊端。本文根据笔者近年来对支抗的系列研究结果,阐述了最大支抗传统概念的模糊性、定量测量支抗丧失时常被忽略的问题及解决方法,并讨论了最大支抗的限度问题。     

全身放射损伤对皮肤伤口组织细胞的影响及康复新的促愈作用(英文)

目的研究全身放射损伤对皮肤伤口组织细胞的影响及康复新(W11-α12)的促愈作用,探讨放创复合伤时创伤难愈的机制与促愈措施。方法100只雄性健康昆明种小鼠,体质量(20±2)g,以随机数字表法设单纯创伤(单伤)和全身放射损伤合并创伤(复合伤)组。小鼠6Gy照射后,于背部造成两个皮肤切口伤,观察伤后伤口愈合速度、伤口炎细胞、成纤维细胞和毛细血管数量及表皮细胞中碱性成纤维细胞生长因子(bFGF)含量的变化。结果伤后3,5,7d,单纯创伤组(单伤组)伤口愈合速度(%)为63.22,83.47,94.61,放创复合伤组(复合伤组)则显著降低(t=7.84,7.26,8.18,P<0.01),分别为52.67,69.18,82.88。伤后1,3,5d,复合伤组伤口炎细胞数量分别比单伤组下降26.53%,62.11%,38.91%;成纤维细胞数量下降43.73%,52.70%,25.32%;毛细血管数量在伤后5d降低55.97%;应用康复新后,两伤组均见伤口愈合速度加快、细胞数量增加,但复合伤组的增幅要大于单伤组。伤后3,5d,复合伤组表皮细胞bFGF含量比单伤组低59.15%和36.15%,当应用康复新后,复合伤组增幅分别比单伤组高35.90%和10.97%。结论伤后伤口局部细胞数量减少和表皮细胞中bFGF含量下降是合并放射损伤后创面难愈的重要原因之一,康复新对伤口的细胞游走、增殖及表皮细胞分泌bFGF的功能具有促进作用。     

山东济宁市淡色库蚊抗药性的测定及杀虫剂配方的筛选

目的为了延缓和克服蚊虫抗性的发生和发展,更好地选用卫生杀虫剂控制蚊虫,测定了常用杀虫剂复配对采自济宁市部分地区现场淡色库蚊幼虫的敏感性及增效作用。方法采用WHO生物测试法,计算LC50、回归方程、增效系数。结果DDVP+三氯杀虫酯、残杀威+三氯杀虫酯复配共毒系数分别为123.24～212.40和171.80～335.80,显示出较好的增效作用。DDVP+残杀威复配效果较差。结论当淡色库蚊产生抗药性后,采用有机磷或氨基甲酸酯类杀虫剂与有机氯类杀虫剂混用的方法,能取得较好效果。     

GPC3真核表达载体的构建及其对肝癌细胞功能的影响

目的：通过构建GPC3增强型绿色荧光蛋白真核表达载体，研究磷脂酰肌醇蛋白聚糖-3（Glypican-3，GPC3）促细胞增殖效应的影响，探讨GPC3基因对肝癌细胞侵袭和转移能力的影响。方法：应用基因重组技术及限制性内切酶酶切构建并鉴定pEGFP-IRES-N1-GPC3增强型绿色荧光蛋白真核表达载体，经脂质体Lipofectamine^TM 2000介导转染BEL-7404后，通过G418筛选出抗性克隆，应用逆转录-聚合酶链反应（RT—PCR）检测GPC3 mRNA在真核细胞中的表达，并在激光共聚焦显微镜下观察目的蛋白在真核细胞内的表达情况，采用免疫荧光法和流式细胞仪检测GPC3对细胞增殖效应的影响。Transwell小室实验检测BEL-7404肝癌细胞的侵袭能力。结果：限制性内切酶酶切分析、重组质粒测序鉴定表明为正确重组子，荧光显微镜下可见转染的真核细胞胞膜区发出强绿色荧光，RT-PCR法表明GPC3在真核细胞中成功表达，转GPC3的BED7404肝癌细胞与对照组相比有促细胞增殖及侵袭和转移效应。结论：构建完成真核表达重组质粒pEGFP—IRES-N1-GPC3；GPC3基因在BEL-7404中成功表达；GPC3可促进肝癌细胞的增殖，其通过增加细胞的侵袭能力而促进肝癌的转移。