一株产生苝醌类化合物真菌的化学成分研究

目的研究菌寄生菌属真菌（Hypomyces (Fr.) Tul. sp.）菌丝体的化学成分。方法用硅胶柱色谱法进行分离纯化，根据理化性质和光谱数据进行结构鉴定。结果从菌丝体中分得两个化合物，命名为hypomycin C（I）和hypomycin D（II)。结论I和II均为新化合物。     

百部生药学研究——Ⅸ.中国百部属植物块根中总生物碱的测定与评估

An acid-dye colorimetric method was reported for the determination oftotal alkaloids in 53 samples of Baibu drugs from their growing destricts in 14 provincesand municipalities and its average recovery and its linear range were 96. 1% ( CV <4%)and 20～150μg respectively. The relationship between the total alkaloid content, geogra-phical origins and morphology were discussed. The results showed that: 1. the content of totalalkaloids of stemona was 0.26～3. 1 %; 2. that of Stemona sessilifolia was 0.26～2.17%with the highest content of the sample from Nanyang country in Henan Province; 3.that of S. japonica was 0.83～1.43%; 4. that of S. tuberosa was 0.53～3.1% with thehighest content of sample from Hengyang in Hunan Province; 5.that of S.parviflora was 0.22～0.74% with the highest content of sample from Qongzhong in Hainan Province;and 6.thatof more yellow, solider and stronger samples was higher than that of any other samples.Howerver, that of all bigger samples in shape was not higher than that of smaller ones.     

湖北贝母化学成分的研究 Ⅴ、湖贝新的分离和鉴定

A new C-nor-D-homosteroid alkaloid of 22,27-imino-17, 23-oxidojervane skeleton was isolated from the bulb of Fritillaria hupehensis Hsiao et K. C. Hsia by column chromatographic techniques, C₂₇H₄₁NO₃, mp257～259℃, named hupehenisine.On the basis of study of the UV. IR. ¹H-NMR and EI-mass spectra of hupehenisine and its diacetyl derivative, the structure of hupehenisine has been established as 22,27-imino-17,23-ozido-5α, 6-dihydrojerv-12-en-11-oxo-3β-ol (Ⅶ).     

甘草叶中甘草宁P-3′-甲醚的分离和鉴定

Two flavonoids were isolated from the leaves of Glycyrrhiza uralensis Fisch (Licorice, Leguminosae). On the basis of physico-chemical properties and spectroscopy (UV, ¹HNMR and MS), a new compound was elucidated as 3,5,7,4′-tetrahydroxy-3′-methoxy- 6-isoprenyl flavone (gancaonin P-3′-methylether) and another known compound was identified as 8-C-prenyleriodictyol.     

丹参冠瘿组织的生长和总丹参酮的积累动态

AIM　To determine the dynamics of growth and total tanshinones accumulation in crown gall cultures of Salvia miltiorrhiza in MS and 67-V liquid media. METHODS　Fresh, dry weight and total tanshinones yields in the cultures and in the medium were determined every 5 days in crown gall suspension cultures. RESULTS　In MS medium, the logarithmic growth phase of crown gall cultures in S.miltiorrhiza was from the 5th to 30th days, and the stationary growth phase was from the 30th to 35th days. From the 25th to 30th days, physiological activity of crown gall cultures was higher and their growth was better. However, in 67-V medium, the logarithmic growth phase of crown gall cultures was from the 10th to 25th days, and the stationary growth phase was from the 25th to 35th days. Total tanshinones were largely accumulated in the cultures and in the medium after 25 days. The total tanshinones yield (60 mg.L^-1) was reached at the 35th day. CONCLUSION　Knowing the regularity of the growth and total tanshinones accumulation in crown gall cultures of S.miltiorrhiza will be helpful to take proper regulative measures in order to obtain the maximum total tanshinones yield.     

宁国贝母中宁贝新甙的分离和结构鉴定

A new steroidal alkaloidal glucoside, C₃₄H₅₇NO₇, mp 284～286℃, named ningpeisinoside, was isolated from the bulb of Fritillaria ningguoensis S.C. Chen et S F. Yin. Based on preparation of derivatives and IR, MS, ¹HNMR, ¹³CNMR spectral studies, the structure of ningpeisinoside has been established as N- methyl- 5α- veratranine-6-oxo-3β-O-β-D-glucoside (Ⅰ).     

浙贝母化学成分的研究——Ⅲ.浙贝酮的分离和鉴定

A new C-nor D-homo steroidal alkaloid named zhebeinone was iso-lated from the bulbs of Fritillaria thunbergii Miq..Its structure was determined by spe-tral analyses and chenmical conversion as 5α, 14α-cevanine-3β, 20β-dihydroxy-6-one or C-27 epimer of periminine.     

平贝母的化学成分研究——Ⅱ.平贝碱甙的分离和鉴定

A new C-nor-D-homosteroid alkaloid glucoside, pingpeimine glucoside, was isolated from Fritillaria ussuriensis Maxim. Based on preparation of derivative and spectral studies, the structure of pingpeimine glucoside was elucidated as Ⅰ.     

短刺虎刺中5-羟基虎刺素-ω-乙基醚的分离和结构鉴定

The structure of 5-hydroxydamnacanthol-ω-ethyl ether, a new anthraquinone, isolated from the root of Damnacanthus subspinosus Hand-Mazz (Rubiaceae) was identified to be 3,5-dihydroxy-2-ethoxymethyl-1-methoxyanthraquinone V by means of UV, IR, NMR and MS analyses. The compound was found to be active against Hela cells in culture.     

鲜罗汉果中黄酮甙的分离及结构测定

A new flavonol glycoside named grosvenorine and a known compoundwere isolated from the fresh fruits of Siraitia grosvenori( swingle)C. By means of UV,FAB-MS,¹H-¹H COSY,¹³C-1H COSY and NOE difference ,spectra,the structure of grosvenorinewas established as kaempferol-3-O-α-L-rhafmnopyranoside-7-O[β-D-glucopyranosyl-(1-2)-α-L-rhamnopyranoside].The known compound was identified as kaempferol-3,7-α-L-dirhamnopyra-noside.     

一个新的醌类化合物的结构鉴定

醌类化合物是一类优良的光敏剂 ,有特殊的化学和生物学特性。含　醌类化合物的一些真菌的代谢产物和植物在民间用于治疗胃痛、风湿性关节炎、跌打损伤等症 ,近来发现　醌类化合物不仅可治疗某些皮肤病 ,而且有光敏杀伤肿瘤细胞和抑制爱滋病病毒的作用[1,2 ] 。作者从云南省西北部山区采集到一株丝状真菌 ,由云南大学生物系杨发蓉教授鉴定为子囊菌纲、肉座菌科、菌寄生菌属真菌(ＡｓｃｏｍｙｃｅｔｅｓＨｙｐｏｃｒｅａｃｅａｅＨｙｐｏｍｙｃｅｓ (Ｆｒ .)Ｔｕｌ.ｓｐ .) ,经驯化 ,实验室内培养成功。本文从该菌固态发酵的菌丝体中分得一新…     

HPLC profiling of Phellinus linteus

HPLC chromatograms of MeOH extracts from a fruit body of the wild-grown P. linteus (natural fruit body), from cultivated fungus (cultivated fruit body), and from the cultured mycelia were compared. The extract prepared from the natural fruit bodies revealed a typical HPLC profile referred to as type 1 with a major peak corresponding to meshimakobnol A (1) together with two minor peaks of hypholomine B (3) and inoscavin A (4); the cultivated fruit bodies exhibited a profile referred to as type 2 with major peaks corresponding to 3 and 4 and a minor peak of 1, and the cultured mycelia showed a profile referred to as type 3 without any of these peaks. We also analyzed HPLC chromatograms of commercial products of P. linteus obtained in the markets. Most of the products claimed to be natural fruit bodies exhibited type 1 profiles, except for one product having an intermediate HPLC profile between type 1 and type 2. The products claimed to be cultivated fruit bodies and cultured mycelia revealed type 2 and type 3 profiles, respectively. The present results indicate that the HPLC chromatogram of the methanol extract of P. linteus can be used as a fingerprint to identify whether the product is from natural fruit bodies, cultivated fruit bodies, or cultured mycelia.     

棒束孢属菌（Isaria sp.）中游离尿苷,腺嘌呤,腺苷的HPLC测定

本文用ＷａｔｅｒｓＨＰＬＣ系统，Ｒａｄｉａｌ－ＰａｋＣ１８径向加压柱、甲醇－０．０１ｍｏｌ／Ｌ磷酸二氢钾（１０：９０）为流动相定量测定了虫划菌－棒束孢菌丝体中游离尿苷、腺嘌呤、腺苷的含量。检测波长为２５４ｎｍ，方法平均回收率９０．６－９３．１％，相对标准偏差０．９０２－１．５８％。方法简便、灵敏度高。     

天然与发酵培养冬虫夏草中核苷类成分的含量及其变化

目的比较天然和人工培养冬虫夏草中核苷类成分的含量,并考察其变化.方法用高效毛细管电泳法测定天然和人工冬虫夏草中核苷类成分的含量;考察湿热对冬虫夏草中核苷类成分含量的影响.结果人工冬虫夏草的核苷含量明显高于天然冬虫夏草;新采集的天然冬虫夏草中核苷类物质含量极低,而采集日久者核苷类成分含量较高;湿热可明显增加天然冬虫夏草中核苷类物质的含量,但对人工冬虫夏草中核苷类成分的含量无明显影响.结论天然和人工冬虫夏草中核苷类成分存在一定差异,天然冬虫夏草中核苷类物质可能主要来源于大分子核苷(酸)的降解.     

高链孢霉属真菌YD-01菌丝体中化学成分的研究

通过对交链孢霉属真菌(Alternaria sp.)YD-01菌丝体的丙酮提取物进行化学成分研究，从中分离得到3个化合物，经波谱数据分析，鉴定其结构为2,3-二羟基喹啉-4-O-β-D-葡萄糖苷(1)、 3-甲基-吡咯并哌嗪-2,5-二酮(2)和柚皮素(3)，其中化合物1为新化合物。     

真菌Nigrospora sp.Z18-17的化学成分

目的研究Nigrospora sp.Z18-17号菌株代谢产物,对其化学成分进行分离鉴定。方法采用硅胶柱色谱、凝胶柱色谱、HPLC等方法进行分离纯化,通过理化性质和波谱分析确定化合物的结构。结果从Nigrospora sp.Z18-17菌株发酵液和菌丝体中分离得到7个化合物,分别鉴定为:2-羟基-3-(4-羟基苯基)丙酸甲酯[2-hydroxy-3-(4-hydroxyphenyl)propionic acid methyl ester,1]、对羟基苯乙醇α-羟基丙酸酯(4-hydroxyphenethyl 2-hydroxypropanoate,2)、环(脯-缬)[cyclo(Pro-Val),3]、环(脯-脯)[cyclo(Pro-Pro),4]、环(苯丙-脯)[cyclo(Phe-Pro),5]、环(酪-羟脯)[cyclo(Tyr-Hyp),6]、尿嘧啶核苷(uridine,7)。结论化合物1～7均为首次从Nigrospora sp.Z18-17号菌株的代谢产物中分离得到。     

冬虫夏草菌丝体水提醇沉物体外抗肿瘤活性研究

目的 研究冬虫夏草菌丝体水提醇沉物的体外抗肿瘤活性及其机制。方法 采用热水浸提-醇沉法得到水提物,高效液相色谱仪测定水提醇沉物中腺苷的量;采用MTT法测定其抗肿瘤活性,利用流式细胞仪结合碘化丙锭染色法检测其对细胞周期的抑制。结果 实验表明水提醇沉物能抑制人肝癌HepG2细胞株及大细胞肺癌NCI-H460细胞株的增殖,并呈浓度相关性;半数抑制浓度（IC₅₀）值分别为（1.49±0.19）和（1.67±0.27）mg/mL。细胞周期分析表明,水提醇沉物分别阻滞HepG2及NCI-H460细胞周期于G₂/M期、S期,并可诱导上述两种细胞发生凋亡。结论 冬虫夏草水提醇沉物通过阻滞HepG2及NCI-H460细胞周期循环,诱导其凋亡,从而表现出良好的增殖抑制活性。为深入研究冬虫夏草菌丝体水提醇沉物抗肿瘤的机制提供了实验证据。     

天然与发酵培养冬虫夏草中核苷类成分的含量及其变化

目的 比较天然和人工冬虫夏草中核苷类成分的含量,并对其变化进行考察。方法 采用高效毛细管电泳法测定天然和人工冬虫夏草中核苷类成分的含量;考察湿热对冬虫夏草中核苷类成分含量的影响。结果 人工冬虫夏草的核苷含量明显高于天然冬虫夏草;新采集的天然冬虫夏草中核苷类物质含量极低,而采集日久者核苷类成分含量却较高;湿热可明显增加天然冬虫夏草中核苷类物质的含量,但对人工冬虫夏草中核苷类成分的含量无明显影响。结论 天然和人工冬虫夏草中核苷类成分存在一定差异,天然冬虫夏草中核苷类物质可能主要来源于大分子核苷(酸)的降解,腺苷在天然冬虫夏草质量控制中的应用价值尚值得进一步研究。     

海绵共附生真菌Hansfordia sp.次级代谢产物的分离与鉴定

目的从海绵共附生真菌Hansfordia sp.的次级代谢产物中得到具有活性的化合物。方法采用硅胶柱色谱、Sephadex LH-20柱色谱和半制备HPLC色谱分离纯化,根据化合物的NMR、MS波谱数据和理化性质进行结构鉴定。结果从真菌Hansfordia sp.的固体发酵物的乙酸乙酯提取物中分离得到8个单体化合物,分别鉴定为1,3,6-trihydroxy-8-methylxanthone(1)、4-chloro-1,3,6-trihydroxy-8-methylxanthone(2)、6-hydroxymellein(3)、(R)-4ydroxymellein(4)、3-(2-hydroxypropyl)-8-hydroxy-3,4-dihydroisocoumarin(5)、2,5-dimethyl-7-hydroxychromone(6)、(S)-regiolone(7)和cy-clonerodiol(8)。结论化合物1～8全部为首次从Hansfordia属真菌中分离得到。