细辛脑对斑马鱼胚胎发育及运动行为的影响

目的：研究细辛脑对斑马鱼胚胎发育及运动行为的影响。方法以受精后3h（hpf）斑马鱼胚胎为实验模型,分别暴露于不同浓度（25、50、100、200和400μmol/L）细辛脑溶液中,以不加细辛脑为对照组,每隔24 h更换1次细辛脑溶液,分别在24、48、72和96 hpf等时间点用显微镜观察班马鱼胚胎发育形态,记录24 hpf胚胎自主抽动次数,48 hpf心率、畸形率和死亡率,72和96 hpf孵化率和死亡率,利用RT-qPCR检测96 hpf斑马鱼胚胎Sepn1基因表达情况,采用Noldus斑马鱼幼鱼行为视频跟踪系统评价细辛脑暴露对幼鱼运动行为的影响。结果细辛脑各实验组斑马鱼胚胎自主抽动次数随浓度增加而降低,100、200和400μmol/L组与对照组相比有极显著性统计学差异（P＜0.01）;细辛脑各实验组斑马鱼48 hpf心率显著降低,与对照组相比均有显著统计学差异（P＜0.01）;72和96 hpf斑马鱼幼鱼出现了脊柱弯曲、心包水肿、卵黄囊水肿等畸形形态;与对照组相比,100、200和400μmol/L组96 hpf孵化率均下降,具有显著性统计学差异（P＜0.01）;与对照组相比,400μmol/L组死亡率降低,有统计学差异（P＜0.05）;随着细辛脑浓度增加,96 hpf斑马鱼幼鱼运动速度和距离明显减小,200和400μmol/L组与对照组相比均有统计学差异（P＜0.05）;同时200和400μmol/L浓度组活跃度降低,与对照组相比具有显著差异（P＜0.01）;96 hpf时,细辛脑各浓度组胚胎Sepn1基因表达下降,其中100μmol/L组与对照组相比有统计学差异（P＜0.05）,而200和400μmol/L组与对照组相比差异更显著（P＜0.01）。结论细辛脑对斑马鱼胚胎和幼鱼有致畸作用,并且影响其运动行为,建议婴幼儿慎用细辛脑。     

肺气肿兔膈肌力学模式对慢性超低频复合生理频率电刺激的适应性改变

目的研究不同频率慢性电刺激(CES)后肺气肿模型兔膈肌力学模式适应性变化,以了解慢性超低频电刺激对肺气肿兔膈肌力学特征的影响.方法采用木瓜蛋白酶雾化吸入法建立兔肺气肿模型;测定正常对照组、肺气肿组和肺气肿CES组膈肌颤搐收缩张力(Pt)、强直颤搐收缩张力(Po)、峰值张力时间(TPT)、1/2松弛时间(1/2Rt)、疲劳指数(FI)和疲劳恢复指数(FRI).结果①同正常对照组比较,肺气肿组Pt、Po降低(P＜0.01),TPT、1/2Rt延长(P＜0.01),FI、FRI增加(P＜0.01).②同肺气肿组比较,40 Hz和(2.5 40)Hz组Pt、Po明显增加(P＜0.01),TPT、1/2Rt明显缩短(P＜0.01),10 Hz组结果则相反(P＜0.05).40Hz、(2.5 40)Hz、10 Hz组FI、FRI明显下降(P＜0.01).③(2.5 40)Hz组与40 Hz组比较,Pt、Po、TPT、1/2Rt无显著统计学差异(P＞0.05),FI、FRI下降(P＜0.01).结论超低频复合生理频率慢性电刺激[(2.5 40)Hz]可显著提高肺气肿兔的膈肌收缩力,较生理频率慢性电刺激(40 Hz)能使肺气肿兔膈肌抗疲劳能力得到更明显的提高.     

高同型半胱氨酸血症与短暂性脑缺血发作及预后的关系

目的：探讨高同型半胱氨酸（ Hcy）血症与短暂性脑缺血发作（ TIA）和患者预后的关系。方法选取确诊治疗的120例TIA患者（ TIA组）和80例脑梗死患者（脑梗死组）,选取同期体检中心120例健康体检者作为对照组,采用酶联免疫吸附法检测3组的血清Hcy 水平,根据Hcy水平进一步将TIA患者分为Hcy≥15.0μmol／L组和＜15.0μmol／L组,随访1年,比较TIA及脑梗死事件,采用logistic回归分析TIA发作的相关危险因素。结果 TIA组和脑梗死组血清Hcy水平分别为（15.65±2.65）μmol／L和（16.88±3.67）μmol／L,均明显高于对照组,差异有统计学意义（P＜0.05）,TIA组与脑梗死组间比较差异无统计学意义（P＞0.05）;脑血管疾病的发生发展与高Hcy、高血压、高血脂等因素有关,差异有统计学意义（ P＜0.05）;logistic回归分析显示高Hcy、高血压、高血脂是TIA发病的独立危险因素（OR＝1.65,OR＝3.22,OR＝4.15,P＜0.05）;Hcy≥15.0μmol／L的TIA患者1年内再发TIA和脑梗死、发作持续时间≥30 min的比例均明显高于Hcy＜15.0μmol／L者,差异有统计学意义（ P＜0.05）。结论高Hcy与TIA复发次数、持续时间均密切相关,血清Hcy升高提示患者预后较差。     

胰岛素泵强化治疗对中期糖尿病肾病尿蛋白的影响

目的 观察不同胰岛素给药方法对中期糖尿病肾病尿蛋白的影响.方法 将98例中期糖尿病肾病患者随机分为胰岛素泵治疗组（A）、胰岛素笔治疗组（B）和口服药物组（C）,A组予诺和灵R胰岛素泵持续皮下注射控制血糖,B组应用诺和灵R3餐前皮下注射控制血糖,C组应用瑞格列奈1～2mg tid po治疗,4周后观察疗效,比较3组治疗前后24h尿微量白蛋白排泄率（UAER）、肌酐（Cr）的变化.结果 A组治疗后UAER较治疗前差异有统计学意义（P＜0.001）,治疗后UAER小于200μg/min的例数明显增多（P＜0.001）,肌肝（Cr）差异无统计学意义（P＞0.05）,但治疗后肌肝（Cr）小于100μmol/L的例数明显增加（P＜0.05）.B组治疗后（UAER）较治疗前差异无统计学意义（P＞0.05）,但治疗后UAER小于200μg/min的例数增多,较治疗前差异有统计学意义（P＜0.05）,CR组间数值与例数差异都无统计学意义（P＞0.05）.C组治疗后（UAER）较治疗前无明显差异（P＞0.05）,治疗后UAER小于200μg/min的例数亦无明显增多（P＞0.05）,肌肝（Cr）组间数值与例数差异都无统计学意义（P＞0.05）.结论 胰岛素强化治疗能降低中期患者尿微量白蛋白,而胰岛素泵应用效果更为理想.药物对降低中期患者尿微量白蛋白无效果.     

N-甲基-D-天门冬氨酸对离体培养人海马神经干细胞增殖的影响

目的研究N-甲基-D-天门冬氨酸（NMDA）对离体培养的人胚胎海马神经干细胞（NSCs）增殖的影响。方法取胎龄8～12周人胚脑海马,分离、培养、传代、鉴定NSCs,取传代培养的NSCs随机分为以下5组：对照组、1μmol/L NMDA组、10μmol/L NMDA组、30μmol/L NMDA组、100μmol/L NMDA组。每组取8孔,对照组使用正常培养基培养,各NMDA组加入不同剂量的NMDA,放在培养箱中继续培养。各组细胞分别在3 d、7 d和12 d后采用MTT法测定吸光度（A）值。结果在NMDA作用3 d和7 d时,100μmol/L NMDA组的A值显著高于对照组（均P〈0.05）;其他各组与对照组相比,以及不同浓度NMDA组间相比,差异均无统计学意义（均P〉0.05）。NMDA作用12 d时,30μmol/L和100μmol/L NMDA组的A值显著高于对照组（均P〈0.05）;其他各组与对照组相比以及不同浓度NMDA组间相比,差异均无统计学意义（均P〉0.05）。结论一定条件下NMDA可以促进离体培养人胚胎海马NSCs增殖。     

促红细胞生成素对阿霉素致大鼠膈肌损伤的保护作用

目的:探讨促红细胞生成素(erythropoietin,EPO)对阿霉素致大鼠膈肌损伤的保护作用。方法:雄性SD大鼠30只,随机均分为对照组、模型组、EPO组。应用离体灌流大鼠膈肌肌条方法,分别测量单收缩张力(Pt)、最大强直张力(Po)、峰值收缩时间(CT)、半舒张时间(1/2RT)、张力最大上升速率(+dT/dtmax)、张力最大下降速率(-dT/dtmax)、张力-频率曲线变化,同时测定膈肌组织超氧化物歧化酶(SOD)活力和丙二醛(MDA)的含量。电镜观察膈肌细胞超微结构变化。结果:模型组大鼠膈肌Pt、Po、+dT/dtmax、-dT/dtmax均明显低于对照组(P0.01),EPO组大鼠膈肌的Pt、Po、+dT/dtmax、-dT/dtmax与对照组差异均无统计学意义(P0.05)。模型组的CT、1/2RT均明显长于对照组及EPO组(P0.01)。予10、20、40、60、100 Hz频率的方波电压刺激膈肌时,模型组大鼠膈肌张力均明显低于对照组和EPO组(P0.01)。与对照组比较,模型组大鼠膈肌组织的SOD活力明显降低,MDA含量明显升高(P0.01),EPO组的SOD活力提高,MDA含量与对照组比较差异无统计学意义(P0.05)。电镜显示阿霉素导致膈肌细胞肌纤维肿胀,线粒体空泡化,EPO改善阿霉素造成的损伤。结论:阿霉素导致大鼠膈肌氧自由基生成增多,清除减少,收缩功能减低;EPO对阿霉素所致的膈肌损伤有保护作用。     

连续性与间歇性肾替代疗法治疗肾衰竭临床对比分析

目的：对肾衰竭患者给予连续性和间歇性肾替代疗法的效果。方法选取肾衰竭患者86例,随机分为2组,各43例。对照组行间歇性肾替代疗法治疗,观察组行连续性肾替代疗法治疗,对比2组临床治疗效果。结果观察组治疗有效率为95.35%,高于对照组的76.74%,差异有统计学意义（P＜0.05）;观察组治疗后Scr、BUN分别为（401.02±138.32）μmol/L、（19.30±4.51）mmol/L,低于对照组,Ccr、β2-MG分别为（23.79±10.93）μmol/L、（27.98±7.64）mg/L,高于对照组,差异有统计学意义（P＜0.05）;观察组治疗后平均动脉压和心率分别为（101.1±20.2）mmHg、（88.4±12.5）次/min,低于对照组,中心静脉压为（15.7±7.5）mmHg,高于对照组,差异有统计学意义（P＜0.05）。结论连续性肾替代疗法治疗肾衰竭患者可明显提高临床疗效,改善肝肾功能,减轻临床症状,降低平均动脉压和心率。     

糖尿病大鼠膈肌功能损伤及钙调控蛋白基因表达的改变

目的研究糖尿病大鼠膈肌功能及钙调控蛋白基因表达的变化,探讨糖尿病大鼠膈肌功能损伤的发生机制。方法使用链
脲佐菌素建立糖尿病大鼠模型,SD大鼠随机分为糖尿病组（随机血糖≥16.7 mmol/L）和正常对照组,造模后4周、8周测定大鼠
体质量和膈肌/体质量比值,生化指标检测各组大鼠空腹血糖和膈肌组织线粒体琥珀酸脱氢酶（SDH）活性;应用体外灌流大鼠
膈肌条的方法,测定单收缩张力、最大强直张力、峰值收缩时间、半舒张时间、张力-频率曲线,电镜观察大鼠膈肌超微结构,采用
RT-PCR检测大鼠膈肌肌质网钙泵（SERCA）和受磷蛋白（PLB）mRNA表达。结果（1）与正常对照组相比,糖尿病大鼠体质量和
膈肌质量/体质量比值均明显降低（P<0.01）,膈肌组织SDH的活性显著降低（P<0.01）;（2）在给予大鼠膈肌条10、20、40、60、
100 Hz刺激时,糖尿病大鼠膈肌在各频率下的收缩张力明显低于正常对照组（P<0.01）;膈肌力学指标单收缩张力和最大强直张
力明显降低,峰值收缩时间和半舒张时间明显延长（P<0.01）;（3）超微结构显示糖尿病组大鼠膈肌组织损伤明显,肌纤维断裂,
肌质网扩张,线粒体水肿,数目减少,脊断裂,空泡化或囊泡化,同时可见随病程延长膈肌损伤明显加重;（4）RT-PCR结果提示：
与正常对照组相比,糖尿病组大鼠膈肌SERCA mRNA表达均明显降低（P<0.01）,糖尿病大鼠膈肌SERCA mRNA表达8周组比
4周组明显降低（P<0.01）,糖尿病大鼠膈肌PLB mRNA表达显著增强（P<0.01）。结论糖尿病大鼠膈肌超微结构受到破坏,线
粒体损伤和数目减少,线粒体SDH活性降低,ATP生成减少,膈肌组织SERCA mRNA表达减少和PLB mRNA表达增强,引起
膈肌肌质网摄取Ca2+减少,促成膈肌收缩和舒张功能损伤。
     

姜黄素抑制刺猬信号诱导小细胞肺癌细胞凋亡

目的：探讨姜黄素对小细胞肺癌NCI- H446细胞的作用及可能机制。方法不同浓度（5、10、15μmol/L）的姜黄素作用于小细胞肺癌NCI- H446细胞后,采用MTT法检测细胞增殖,流式细胞仪检测细胞凋亡,Western blot技术检测索尼克刺猬信号（Shh）、脑胶质瘤相关癌基因1（Gli1）的表达水平。结果不同浓度的姜黄素与小细胞肺癌NCI- H446细胞共培养后,浓度为15μmol/L的姜黄素能明显抑制NCI- H446细胞的增殖。与其余各组相比,差异有统计学意义（P＜0.01）。小细胞肺癌NCI- H446细胞经姜黄素处理后,姜黄素15μmol/L与其余各组比较,细胞凋亡率的差异有统计学意义（P＜0.01）。经姜黄素（15μmol/L）处理的NCI- H446细胞,Shh和Gli1表达均明显降低,与对照组相比,差异均有统计学意义（P＜0.01）。结论姜黄素能通过抑制刺猬信号通路,抑制小细胞肺癌细胞的增殖,诱导其凋亡。     

丹参酮ⅡA对人膀胱癌J82细胞增殖、凋亡、侵袭及迁移的影响

目的探讨丹参酮ⅡA在体外对人膀胱癌J82细胞增殖、凋亡、侵袭及迁移的影响。方法以不同浓度的丹参酮ⅡA处理J82细胞,分别采用细胞计数试剂盒（CCK-8）法、膜联蛋白V-异硫氰酸荧光素（AnnexinV-FITC）/碘化丙锭（PI）双标记流式检测法、Transwell侵袭实验及划痕实验检测J82细胞的增殖、凋亡、侵袭及迁移情况。结果丹参酮ⅡA可抑制J82细胞增殖,且呈浓度与时间依赖性（均P＜0.05）。1、3、5滋mol/L丹参酮ⅡA处理组总凋亡率分别为12.23%、14.74%、18.40%,均高于对照组的3.36%（P＜0.05）,且呈浓度依赖性（P＜0.05）。对照组、1滋mol/L组、3滋mol/L组、5滋mol/L组细胞侵袭数比较,差异有统计学意义（P＜0.05）,且呈浓度与时间依赖性（均P＜0.05）。对照组、1滋mol/L组、3滋mol/L组、5滋mol/L组划痕后12、24、36h划痕愈合率比较,差异均有统计学意义（均P＜0.05）,呈浓度依赖性（P＜0.05）。结论丹参酮ⅡA在体外可抑制膀胱癌J82细胞增殖,促进其凋亡,降低其侵袭和迁移能力。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.