颜面皮肤癌中NOS和VEGF的表达及其临床病理意义

目的研究血管内皮生长因子(VEGF)、诱导型一氧化氮合酶(iNOS)和内皮型一氧化氮合酶(eNOS)在颜面皮肤癌中表达的相关性;探讨三者与颜面皮肤癌血管生成和临床病理特征的关系;研究一氧化氮(NO)和VEGF的相互作用及NO在VEGF促肿瘤生长中的作用机制.方法应用免疫组织化学方法检测47例颜面皮肤癌手术切除标本VEGF、iNOS和eNOS的表达,Ⅷ因子相关抗原(FⅧRAg)血管内皮细胞特异性染色计数肿瘤微血管密度(MVD).结果 (1)32例颜面皮肤癌组织表达VEGF,30例表达iNOS,35例表达eNOS;(2)VEGF与iNOS的表达呈正相关,与eNOS的表达无相关;(3)VEGF、iNOS的表达与颜面皮肤癌MVD呈正相关, eNOS的表达与颜面皮肤癌MVD的差异无显著性意义;(4)VEGF的表达与颜面皮肤癌淋巴结转移和肿瘤分化程度及肿瘤浸润深度呈正相关;与临床分期无关.iNOS表达与颜面皮肤癌的分化程度及临床分期和有无淋巴结转移及肿瘤浸润深度正相关;eNOS表达与颜面皮肤癌临床分期和分化程度及有无淋巴结转移及肿瘤浸润深度均无关.结论 (1)iNOS对VEGF的生成和发挥作用的过程有重要影响;(2)MVD随着VEGF和iNOS表达的增强而增加,说明两者对颜面皮肤癌血管生成具有促进作用.     

胃癌组织iNOS、COX-2和VEGF的表达及其与血管生成关系

目的探讨iNOS、COX-2、VEGF在人胃癌组织中的表达及其与胃癌血管生成及临床病理因素之间的关系.方法应用免疫组化S-P法检测67例胃癌组织中iNOS、COX-2、VEGF的表达,根据第八因子相关抗原(FⅧRAg)阳性的血管内皮细胞计数肿瘤组织微血管密度(MVD).结果①iNOS、VEGF阳性表达主要定位于癌细胞胞质;COX-2的阳性表达定位于癌细胞的胞质,部分胞膜见阳性表达;胃癌组织iNOS、COX-2和VEGF高表达率分别为68.7%,76.1%和76.1%;② iNOS、COX-2和VEGF的高表达与胃癌组织的分化程度成正相关;③癌组织MVD 均数为30.84±9.13个/200倍视野,iNOS、COX-2及VEGF高表达组MVD显著高于低表达、无表达组,差异具有显著性意义(P值均<0.05).④ iNOS、COX-2和VEGF高表达与胃癌浸润深度呈显著正相关(P<0.001),iNOS、VEGF的高表达还与五年生存率呈负相关(P<0.01).⑤ iNOS、COX-2的表达均与VEGF的表达呈正相关(r分别=0.527,0.425;P值分别＝0.015,<0.001).结论胃癌组织iNOS、COX-2的表达与肿瘤血管生成密切相关,二者可能与VEGF协同促进肿瘤血管生成,有望成为判断肿瘤恶性潜能的重要生物学指标.     

涎腺腺癌COX-2表达和肿瘤血管生成的研究

目的 探讨环氧合酶-2（COX-2）表达和涎腺腺癌血管生成的关系。方法 免疫组化检测涎腺腺癌COX-2、诱导型一氧化氮合酶（iNOS）、血管内皮生长因（VEGF）表达，并测定肿瘤微血管密度（MVD）。结果 涎腺腺癌COX-2表达与VEGF、iNOS表达密切相关（P〈0．05）。涎腺腺癌COX-2表达阳性组和阴性组MVD差异有统计学意义（P〈0．01）。结论 涎腺腺癌COX-2表达和肿瘤血管生成有关，iNOS和VEGF可能参与COX-2对肿瘤血管生成的促进作用。     

口腔恶性黑色素瘤COX-2表达和肿瘤血管生成的研究

目的探讨环氧化酶-2（COX-2）表达和口腔恶性黑色隶瘤血管生成的关系。方法免疫组化检测口腔恶性黑色素瘤COX-2、诱导型一氧化氮合酶（iNOS）、血管内皮生长因子（VEGF）表达。并测定肿瘤微血管密度（MVD）。结果口腔恶性黑色素瘤COX-2表达与VEGF、iNOS表达密切相关（P〈0.05）。口腔恶性黑色素瘤COX-2表达阳性组和阴性组MVD有显著差异（P〈0．01）。结论口腔恶性黑色素瘤COX-2表达和肿瘤血管生成有关，iNOS和VEGF可能参与COX-2对肿瘤血管生成的促进作用。     

大肠肿瘤COX-2表达和肿瘤血管生成的研究

目的 探讨环氧化酶-2(cyclooxygenase,COX-2)表达和大肠肿瘤血管生成的关系。方法 免疫组化检测大肠肿瘤COX-2表达、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达,并测定肿瘤微血管密度(microvessel density,MVD)。结果 大肠肿瘤COX-2表达阳性组和阴性组MVD有显著差异(P<0.05)。偏相关分析显示COX-2表达和VEG表达相关(校正MVD因素,偏相关系数r=0.801,P<0.01),VEG表达和MVD相关(校正COX-2因素,偏相关系数r=0.392,P<0.01),COX-2和MVD不相关(校正 VEGF因素,偏相关系数r=0.138 0,P>0.05)。结论 大肠肿瘤COX-2表达和肿瘤血管生成有关,VEGF可能参与COX-2对肿瘤血管生成的促进作用。     

术前间质化学治疗对口腔鳞状细胞癌环氧酶2、血管内皮生长因子表达及微血管密度的影响

[目的]探讨术前间质化学治疗对口腔鳞状细胞癌环氧酶2(COX-2)、血管内皮生长因子(VEGF)表达及微血管密度(MVD)的影响.[方法]选择2001年—2009年间接受治疗的高分化口腔鳞状细胞癌患者30例,分为术前静脉化学治疗组(15例)和间质化学治疗组(15例),采用免疫组织化学法检测两组COX-2,VEGF表达水平和MVD值变化.[结果]术前间质化学治疗组COX-2,VEGF表达水平及MVD值明显低于术前静脉化学治疗组(P<0.05),COX-2,VEGF阴性组MVD值明显低于COX-2,VEGF阳性组(P<0.05).[结论]术前间质化学治疗和静脉化学治疗均可能通过抑制COX-2,VEGF及MVD表达从而起到减少肿瘤新生血管生成的作用,间质化学治疗较静脉化学治疗作用更为明显.     

COX-2和VEGF在前列腺癌中的表达及其与肿瘤血管生成关系的研究

目的:探讨环氧化酶-2(COX-2)和血管内皮生长因子(VEGF)在前列腺癌(PCa)中的表达及其与肿瘤血管生成的关系。方法:30例前列腺癌组织,按Gleason评分,将≥7分者列为高Gleason评分组(n=16);<7分者列为低Gleason评分组(n=14)。良性前列腺增生(BPH)20例,BPH并高级前列腺上皮内瘤(PIN)12例。正常前列腺组织(NP)10例。应用免疫组化方法检测各组织中COX-2、VEGF的表达水平及其微血管密度(MVD)。结果:PCa、PIN中COX-2、VEGF的阳性表达率及MVD值高于BPH及NP(P均<0.05),高Gleason评分组中COX-2、VEGF的阳性表达率(81.3%、93.8%)及MVD值(63.4±1.80)高于低Gleason评分组(阳性表达率为42.9%、64.3%,MVD值为51.7±1.56,P均<0.05)。COX-2与VEGF之间呈正相关(r=0.630,P<0.05),COX-2阳性组及VEGF阳性组MVD值(69.3±3.91、72.6±3.63)均高于COX-2阴性组及VEGF阴性组(43.9±2.51、40.6±2.84,P<0.05)。结论:COX-2、VEGF在前列腺癌中均为高表达,且与肿瘤的Gleason评分密切相关,COX-2与VEGF的表达可能有协同作用,共同促进肿瘤血管生成,增加微血管密度。     

环氧合酶-2与食管鳞癌新生血管的关系

目的 探讨环氧合酶-2(COX-2)与食管鳞癌新生血管形成的关系.方法 应用免疫组化方法检测33例食管鳞癌组织和癌旁组织的COX-2、血管内皮生长因子(VEGF)、微血管密度(MVD)的表达水平,分析三者间的相互关系,结合临床病理特征进行分析.结果 COX-2与食管鳞癌浸润深度以及淋巴结转移相关(P<0.05);食管鳞癌组织COX-2阳性组、VEGF阳性组比各自的阴性组MVD水平高(P<0.01),鳞癌组织的COX-2、VEGF表达阳性率以及MVD水平比癌旁组织明显增高(P<0.05). 结论 食管鳞癌肿瘤新生血管形成可能与COX-2、VEGF过度表达有关.     

环氧合酶-2在食管癌中表达及与血管生成关系

目的:研究食管癌中环氧合酶-2(COX-2)、血管内皮生长因子(VEGF)的表达及其与肿瘤微血管密度(MVD)和组织病理特征之间的关系。方法:应用免疫组化和原位杂交的方法分别检测72例食管癌组织和邻近正常食管上皮中COX-2 mRNA、COX-2蛋白、VEGF蛋白和CD31的表达,以CD31作为MVD指标,分析COX-2、VEGF与肿瘤MVD值和组织病理特征之间的关系。结果:在食管癌组织和正常上皮组织中各因子表达率分别为:COX-2 mRNA(77.8%,9.7%)、COX-2蛋白(88.9%,11.1%)、VEGF蛋白(58.3%,18.1%)、MVD值(33.40±6.517,18.22±6.396)。COX-2和VEGF在食管癌组织中出现异常高表达并伴有MVD值增加,而且与肿瘤浸润深度、淋巴结转移和TNM分期呈正相关。结论:COX-2、VEGF在食管癌组织中表达增高并与血管生成关系密切,并且可能参与了肿瘤细胞的浸润和转移;COX-2、VEGF的表达可作为判断食管癌生物学行为的重要指标。     

胃癌组织中COX-2、VEGF和MVD的表达及临床意义

目的 观察环氧合酶-2(COX-2)、血管内皮生长因子(VEGF)和微血管密度(MVD)在正常胃黏膜组织、癌旁组织和胃癌组织中的表达,探讨COX-2的表达与肿瘤血管生成的关系?方法 采用SP免疫组化方法检测30例正常胃黏膜组织、50例癌旁组织和50例胃癌组织中COX-2、VEGF和MVD的表达?结果 正常胃黏膜组织→癌旁组织→胃癌组织中,COX-2的表达强度呈逐渐增加(P<0.01),COX-2的表达与肿瘤的分化程度和淋巴结转移有关(P<0.01),而VEGF的表达和MVD呈逐渐上升趋势,两者表达与肿瘤的分化程度、浸润深度和淋巴结转移有关?COX-2与MVD的表达呈正相关。结论 COX-2的异常表达及其诱导的血管生成与胃癌的分化程度、淋巴结转移密切相关。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…