芪苈强心胶囊中一个孕甾糖苷的NMR数据分析

目的 分离鉴定芪苈强心胶囊活性部位中的指标性成分。方法 采用大孔吸附树脂、硅胶和C18柱色谱的方法分离芪苈强心胶囊活性部位的化学成分,利用1H-NMR和13C-NMR、1H-1H COSY、HSQC和HMBC等多种核磁共振方法鉴定其结构。结果 从芪苈强心胶囊中分离得到1个孕甾糖,结构鉴定为△5-pregnene-3β,16β,20(R)-triol 3-O-［2, -O-acetyl-β-D-digitalopyranosyl-(1→4)-β-D-cymaropyranoside］20-O-［β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl-(1→2)-β-D-digitalopyranoside］(S-4a)。结论 S-4a为首次从芪苈强心胶囊中得到的化合物,对其1H-NMR和13C-NMR信号进行了详细分析,首次报道了其完整的1H-NMR数据。     

中西医结合治疗慢性心衰急性加重患者疗效观察

自2004年6月-2005年6月,本校附属医院内科采用芪苈强心胶囊(石家庄以岭药业股份有限公司生产)配合西药常规治疗(强心、扩管、利尿及对症治疗)治疗慢性心衰急性加重患者24例,取得了较好的疗效,现报道如下.     

芪白平肺胶囊中3种人参皂苷类成分含量测定

目的 建立高效液相色谱法配备蒸发光散射检测器 (HPLC－ELSD) 测定芪白平肺胶囊中人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量,并通过优化ELSD参数,获得最佳分离检测条件。方法 采用色谱柱为Hypersil ODS XB-C8柱(4.6 mm?250 mm,5 μm)和Hypersil ODS XB-C18柱(4.6 mm?250 mm,5 μm),以乙腈为流动相A,以水为流动相B进行梯度洗脱;洗脱程序( 0～40 min,A 19%;40～55 min,A19%～40%),流速为1. 2 mL.min-1,柱温为30 ℃。ELSD参数优化: 漂移管温度分别设为50℃,70℃,90 ℃和110℃,载气流量分别设为1.0 L.min-1,1.2 L.min-1,1.4 L.min-1和1.6 L.min-1。结果 通过对比研究,最终采用Hypersil ODS XB-C8柱作为分析柱,漂移管温度为90℃,载气流量为1.2 L.min-1时,3种人参皂苷的含量检测最佳。人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的加样回收率分别为98.3%、99.1% 和101.8%,RSD分别为0.67%、RSD为1.03% 和1.22%。结论 色谱柱的选择研究和ELSD参数的优化对人参皂苷类成分的含量测定具有一定的指导意义,该方法准确度高,重现性好,亦可用于芪白平肺胶囊的质量控制。     

芪苈强心胶囊治疗心衰临床观察

目的:评价芪苈强心胶囊治疗充血性心力衰竭的疗效及安全性。方法:94例慢性心力衰竭患者分为对照组和治疗组,所有患者根据心衰治疗指南给予强心、利尿、扩血管等西医常规治疗。治疗组在此基础上给予芪苈强心胶囊治疗。治疗组46例,对照组48例,疗程均为4周。观察患者心力衰竭症状和体征的改变。试验完成。结果:治疗组总有效率为89.1%,不良反应发生率为0%;对照组总有效率为70.8%,治疗组疗效高于对照组(P<0.05)。治疗组疗效明显优于对照组。结论:芪苈强心胶囊治疗慢性充血性心力衰竭有明显疗效     

芪苈强心胶囊治疗慢性充血性心力衰竭作用机制的网络药理学研究

目的:运用网络药理学分析芪苈强心胶囊治疗慢性充血性心力衰竭的作用机制.方法:从TCMSP平台获取芪苈强心胶囊的化学成分和作用靶点,利用TTD、Drugbank、DisGeNET数据库筛选慢性充血性心力衰竭(CHF)的靶点,将疾病靶点与药物靶点进行Venn分析,筛选二者共同靶点,应用string平台构建蛋白-蛋白相互作用网络,利用Cytoscape软件构建中药-化学成分-靶点和中药-化学成分-靶点-通路-疾病网络.最后,通过DAVID在线分析数据工具对共同靶点进行GO富集分析和KEGG通路富集分析.结果:芪苈强心胶囊涉及11味中药,140种化学成分及329个作用靶点,与治疗CHF相关的关键靶点80个,共涉及480条生物条目和86条信号通路.芪苈强心胶囊中的槲皮素、木犀草素、β-谷甾醇、山奈酚、川陈皮素、黄芩素、丹参酮ⅡA、人参皂苷Rh2等有效化学成分,可能通过作用于IL6、VEGFA、TNF、EDN1、FOS、INS等关键靶点,调节HIF-1、TNF、cGMP-PKG、PI3K-Akt、AMPK等多条信号通路治疗CHF.结论:芪苈强心胶囊通过多成分、多靶点、多途径协同作用治疗CHF.     

芪苈强心胶囊佐治慢性心力衰竭疗效观察

目的:观察芪苈强心胶囊佐治慢性心力衰竭的疗效。方法:将84例左心室收缩功能不全,心功能NYHAⅡ~Ⅳ级,左心室射血分数(LVEF)<45%的患者按入院顺序随机分为芪苈强心胶囊组和常规治疗组各42例。芪苈强心胶囊组在常规治疗基础上加用芪苈强心胶囊,比较2组疗效。结果:芪苈强心胶囊组的疗效优于常规治疗组(P<0.01);LVEF、6min步行距离治疗后比治疗前均有较大改善(P<0.01),芪苈强心胶囊组改善更为明显,且未见不良反应。结论:芪苈强心胶囊佐治慢性充血性心力衰竭可以取得较好疗效。     

清脑宣窍方有效部位的化学成分研究(Ⅰ)

目的对清脑宣窍方中有效部位化学成分进行系统研究.方法大孔树脂、聚酰胺、硅胶等层析方法分离,波谱法鉴定结构.结果从清脑宣窍方有效部位中分离得到9个化合物,分别为:①熊果酸,②人参皂苷Rg1,③人参皂苷Rh1(R),④人参皂苷Rh1(S),⑤人参皂苷Re,⑥人参皂苷Rb1,⑦栀子苷,⑧三七皂苷R1,⑨Genipin-1-O-β-gentiobioside.结论以上9个化合物均为首次从复方清脑宣窍方中分离得到的已知化合物.     

三七果化学成分的研究

目的 研究三七果的化学成分。方法 采用硅胶色谱和凝胶色谱以及重结晶等方法,从云南文山三七果的70%乙醇提取物中分离得到14个化合物。通过理化性质和波谱学分析鉴定化学结构。结果 分离得到了14个化合物,分别鉴定为β-谷甾醇(1)、20(S)-人参二醇(2)、20(S)-原人参二醇(3)、20(R)-人参皂苷Rh1(4)、20(R)-人参皂苷Rh2(5)、胡萝卜苷(6)、20(R)-人参皂苷Rg1(7)、20(S)-人参皂苷Rg2(8)、20(R)-人参皂苷Rg3(9)、腺嘌呤核糖核苷(10)、人参皂苷Re(11)、人参皂苷Rd(12)、人参皂苷Rb1(13)、人参皂苷Rb3(14)。结论 化合物1～14均为首次从三七果中分得,其中化合物10为首次从人参属中分得。     

人参果中一个新的天然化合物的分离

目的研究人参浆果的化学成分。方法采用溶剂提取和柱色谱分离等方法进行分离和纯化,根据化合物的理化常数和波谱数据鉴定其结构。结果从人参浆果分离得到12个化合物,分别鉴定为:苯甲酸(Ⅰ)、异人参皂苷-Rh3(Ⅱ)、人参皂苷-Rh2(Ⅲ)、人参皂苷-Rh1(Ⅳ)、人参皂苷-Rg1(Ⅴ)、人参皂苷-Re(Ⅵ)、人参皂苷-Rd(Ⅶ)、人参皂苷-Rc(Ⅷ)、人参皂苷-Rb2(Ⅸ)、人参皂苷-Rb1(Ⅹ)、β-谷甾醇(Ⅺ)和20-O-β-D-吡喃葡萄糖基-20(S)-原人参二醇,命名为化合物K(ⅩⅡ)。结论化合物K是一个新的天然产物。     

脑得生片物质基础的HPLC-DAD-MS~n分析

目的:明确脑得生片的化学物质基础。方法:采用高效液相-二极管阵列-质谱(HPLC-DAD-MSn)联用技术检测并鉴定其主要化学成分。色谱柱为Agilent Zorbax SB-C18(4.6 mm×250mm,i.d,5μm),流动相为0.05%甲酸-水和乙腈,流速为0.5 ml.min-1。结果:在脑得生片中共检测到43个化学成分,通过比对紫外吸收图谱、标准品ESI-MSn质谱图提供的分子量及结构式,并结合文献报道,推断鉴定出其中22个化合物,分别为羟基红花黄色素A、4’-O-葡萄糖基葛根素、3’-羟基葛根素、大豆苷元-8-C-芹菜糖基(1-6)葡萄糖苷、葛根素、6″-O-木糖基葛根素、6″-O-芹菜糖基葛根素、3’-甲氧基葛根素、3’-甲氧基-6″-O-木糖葛根素、大豆苷、5,7-三羟基异黄酮-7-糖苷、葛根苷A、三七皂苷R1、人参皂苷Re、人参皂苷Rg1、大豆苷元、鹰嘴豆素A、人参皂苷Rb1、人参皂苷Rc、人参皂苷Rb2、人参皂苷Rb3和人参皂苷Rd。结论:初步明确了脑得生片的化学物质基础,为进一步提高脑得生片的质量控制标准提供了科学数据。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…