Frequent Import and Multiple Sources of Dengue Fever have Changed the Epidemic Situation of the Disease in Fujian Province,China

Objective The aim of this study was to update the epidemic situation of dengue fever(DF) and provide new insights for the consideration of disease control in Fujian province,China.Methods Details about DF cases in Fujian reported during 2004–2017 were collected and analyzed.The envelope(E) genes of isolates of dengue virus(DENV) were sequenced for phylogenetic analysis.Results The number of imported DF cases had increased dramatically since 2013,and the source regions expanded from Southeast Asia to South Asia,America,Oceania,and Africa,as well as the surrounding provinces.This resulted in local outbreaks and indigenous cases of DF that occurred more frequently,with 10 of 13 local outbreaks and 85.9%(1,252/1,458) of indigenous cases reported in2013–2017.Compared with only two coastal cities before 2013,four coastal and one inland city in 2013–2017 experienced the local DF outbreaks.The phylogenetic analysis of E genes confirmed that the import of DENV,not only from abroad but also from the surrounding provinces,played an important role in dissemination and local outbreaks of DF in Fujian.Conclusions The frequent import of DF cases from not only abroad but also the surrounding provinces resulted in increased incidence,frequent local outbreaks,and expansion of distribution in Fujian in recent years.There is a need for urgent measures to improve disease control in this province.     

Trends in Suicide by Poisoning in China 2000-2006： Age, Gender, Method, and Geography

Objective This study analyzed patterns of suicide and suicide attempts by poisoning as reported through a national poison control system for the purpose of improving intervention and prevention. Methods During the period of 2000 to 2006, 6440 cases of poisoning suicide were reported to the telephone consultation service system of The National Center for Poisoning Control （Chinese Center for Disease Control and Prevention）. Among these records, 4728 cases had completed data for this analysis in terms of age, sex, trend of time and location, and type of poisons. Results There were 60.6% female cases with the age from 10 to 90 years old. The age of cases from 20 to 39 years accounted for 54.5% of all age groups. Both the numbers and percentage in record related to poisoning consultation of oral poisoning suicide showed an increasing tendency during the 7 years. In particular, there was a drastic increase from 2004 to 2006. In addition, the high frequency of cases occurred from May to October. Hebei, Shandong, Henan, and Anhui Provinces had the highest number of cases. Pesticide poisonings were the most common method in these cases of consultation for suicide and suicide attempts. Conclusion This study describes epidemiological characteristics in the oral poisoning suicide cases and provides scientific basis for suicide prevention interventions.     

Acute bacterial meningitis in children in Hefei, China 1990-1992.

OBJECTIVE To obtain etiologic and epidemiologic information about bacterial meningitis, especially the H influenza type B (Hib), from a medium-sized city, Hefei, China.

METHODS Data were collected prospectively over 3 years, from 1990 to 1992 by a well-organized group including 13 hospitals. All children with a clinical diagnosis of acute bacterial meningitis were enrolled and the specimens were taken for the etiologic studies. CSF and blood were tested by standard bacteriologic technique. CSF, blood and concentrated urine were tested directly for detection of antigen by countercurrent immuno-electrophoresis (CIE). Data were analyzed by epidemiologic methods.

RESULTS Bacterial culture and CSF Gram's staining were positive only in 13.3% and 11.7%, respectively. Bacterial antigen detection was positive in up to 90% by CIE which was more sensitive than bacterial culture (chi 2 = 67.7, P < 0.005). The annual incidence of acute bacterial meningitis in the city is calculated as 9.3 cases/100,000 children from 1 month to 15 years of age and 19.2 cases/100,000 children from 1 month to 5 years of age. Hib meningitis accounted for 51.7%, N. meningitis (Nm) for 38.3%, and S. pneumoniae (Sp) for 8.3%. There was no significant seasonal variation. Of the patients, 76.7% were children under 5 years of age, and 51.7% under 1 year of age. The case fatality rate was 11.7% for all bacterial meningitis, 9.7% for Hib, 17.4% for Nm and 20% for Sp. A total of 22.6% of survivors suffered from neurological or psychological problems.

CONCLUSIONS Using antigen detection combined with bacterial culture, we could make an etiologic diagnosis in up to 90% of the patients in this group. Hib, Nm and Sp were the predominant pathogens, which was similar to the findings in other countries. Hib was the most common cause of bacterial meningitis, but the incidence was much lower than in most parts of the world.

     

Hospitalized patients with novel influenza A (H1N1) virus infection: Shanghai, June–July 2009

Background From late May 2009, sporadic imported cases of novel influenza A （HIN1） were continuously confirmed in Shanghai, but there were few reports on its clinical presentation in China. The aim of the study was to investigate the demographic and clinical features of the laboratory-confirmed cases and the treatment with oseltamivir. Method We performed a retrospective study in the Shanghai Public Health Clinical Center （SHAPHC）, reviewing the medical records of the laboratory-confirmed patients derived from June 10 to July 20, 2009. Results A total of 156 cases were enrolled, of whom 152 had a history of recent travel. The mean age was 22.6 years and 89 cases （57.1%） were males. The most common symptoms were fever, cough, and sore throat, with children more likely to run a temperature above 38.5℃ than adults. The mean leucocyte count was 5.4×10^9/L, the mean neutrophil count 3.2×10^9/L and the mean lymphocyte count 1.4×10^9/L. Other findings included a normal range or elevated level of C-reactive protein （CRP） and glutamic-pyruvic transaminase and a normal or decreased level of prealbumin; the levels of prealbumin and CRP were significantly lower in the children than in the adults. Fifty-two patients had abnormal chest CT results, with small unilateral or bilateral pulmonary infiltrates, axillary and mediastinal lymphadenopathy and local pleural thickening, while no cases showed symptoms of hypoxia. All the patients received oseltamivir and recovered without complications, but the duration of fever and virus shedding were significantly longer in the children than in the adults. Conclusions Travel-related circulation may be an important reason for the H1N1 epidemic in the non-epidemic areas, and the virus caused mild respiratory symptoms. The infection in children was more severe in terms of prealbumin levels, temperature, the duration of fever and virus shedding. Oseltamivir was effective for H1N1, but more effective in the adults than in the children.     

Urban-rural comparison of HBV and HCV infection prevalence in eastern China

The present study was initiated to make and urban-rural comparison of the prevalence of cases positive to hepatitis B and C virus(HBV and HCV,respectively)infection markers in densely populated eastern half of China.For this purpose.10 survey sites were selected,i.e.,six sites in urban areas(the city group;Beijing,shangahi and four provincial capitals)and four sites in rural areas(the village group;one village each in Jilin and Shandong provinces,and two villages in Shaanxi Province),About 50 adult women per site volunteered to participate,from whom 494 valid bllod samples were collected.Positivities to HBsAg(HBsAg^ ),anti-HBs(anti-HBs^ )and antiHBc(anti-HBc^ )were examined by RIA methods.and that to anti-HCV(anti-HCV^ )by either EIA or RIA.Those positive to any one of the three HBV infection markers were taken as HBV infection-positive(HBV^ ).The prevalence of HBsAg^ ,HBV^ and anti-HBc^ was 8%,70%and 2.7% in the city group,and 8%,65% and 2.0%in the village group,and no significant difference was found between the two groups.The overall prevalence was 8% for HBsAg^ ,68% for HBV^ ,and 2.4% for anti-HVC^ ,The results were discussed in reference to some 20 papers each on HBV^ and anti-HCV^ prevalence in China published since(1991),The reviewing of these papers of anti-HCV was low(well below 5%),and that no substantial difference was found between the rural and urban populations.     

Validity of diagnostic evidence for deceased cases in hospitals

Objective To determine the validity of the diagnostic evidence for deceased cases in hospitals. Methods All information collected from medical records of the deceased cases in tertiary care health facilities was input into ottr database. Four diagnosis levels were determined based on level of diagnostic evidence： level Ⅰ was based on autopsy, pathology or operative exploration, level Ⅱ on physical and laboratory tests plus expert clinical judgment, level Ⅲ on expert clinical judgment, level Ⅳ on postmortem assumptions. After the diagnostic evidence of each deceased case was reviewed by a panel of three experts, the diagnostic level of each diagnosis was determined. Results Among the 2102 medical cases for verbal autopsy study, only 26 （1.24%） afforded diagnostic evidence for level Ⅲ Among the level Ⅲ evidence-based cases of death, the major causes of death were cardiovascular diseases, respiratory diseases, and gastroenterological diseases. According to some special symptoms and medical histories, these cases could be diagnosed by comprehensive clinical judgment. Only one case met the criteria for level Ⅳ. Conclusion Level Ⅰ diagnostic evidence is hard to attain in China because of the traditional concept and economic restriction. The causes for 2101 deaths can be validated by level Ⅱ or Ⅲ diagnostic evidence.     

Analysis of DNA Ploidy, Cell Cycle and Ki67 Antigen in Nasopharyngeal Carcinoma by Flow Cytometry

The expression of DNA ploidy, the cell cycle and Ki67 antigen in nasopharyngeal carcinoma (NPC) were studied and their relationship with the clinical biological behaviors and prognosis of NPC was evaluated. Biopsied specimens of NPC were made into cell suspension. By using cytometric double labeling Ki67 and DNA method, the expression of DNA ploidy, the cell cycle and Ki67 antigen were analyzed. The patients were followed up for about 3 years and the relationship between the above mentioned parameters and the clinical biological behavior and prognosis of NPC were evaluated. Of the 62 cases of NPC, the DNA aneuploid accounted for 29.03 %. The S phase cells accounted for 0 to 54 % in the cell cycle and the positive expression of Ki67 ranged from 0 to 52%. There were 40 cases of LPI (64.5%) including 15 negative cases and 22 cases of HPI (35.5%) respectively. The DNA anneploid content was positively related to the S phase cells. The patients having a low expression of Ki67 or DNA aneuploid in tumor cells were not sensitive to chemotherapy, liable to metastasis to distant organs and had a poor prognosis, while Ki67 showed no correlation with DNA ploidy and the cell cycle. It was suggested that DNA ploidy and Ki67 could be used as an independent and objective marker to evaluate the radiosensitivity and prognosis of NPC.     

Primary tracheobronchial amyloidosis in China: Analysis of 64 cases and a review of literature

Primary tracheobronchial amyloidosis (TBA) is a rare pulmonary disease.A systematic review was performed on 64 cases of primary TBA in China and progress in the diagnosis and treatment of this disease is discussed.The Chinese biological and medical databases from 1970 to 2010 were searched and 75 cases of complete clinical and pathological data were identified.The clinical characteristics of the disease were summarized and longitudinal comparisons were made of diagnostic and treatment methods over time.The results showed that the morbidity associated with primary TBA has increased over recent years.The clinical manifestations were non-specific.Progressive dyspnea, cough and sputum were the most common symptoms.The percentage of patients undergoing computed tomography (CT) scan has increased over the years.The bronchoscopy and transbrochial lung biopsy (TBLB) were usually sufficient to establish the diagnosis.Treatment was reported for a total of 44 cases.Bronchoscopic Nd:YAG laser irradiation, argon plasma coagulation (APC) and drugs administration such as steroids and colchicines were reported to be effective in some patients.It is concluded that the demographic characteristics and clinical manifestations of primary TBA patients in China are largely consistent with findings reported in other countries.Dramatically more cases were reported in recent years, mainly due to the extensive application of bronchoscopy since 1990s.Chest CT scan provides important clues for the diagnosis of the disease.The definite diagnosis was confirmed by bronchoscopic findings and Congo red staining of biopsy specimen.Bronchoscopic Nd:YAG laser irradiation, argon plasma coagulation (APC) and drugs administration, such as steroids and colchicines were reported to be effective in some patients.     

A survey of blindness and poor vision in leprosy patients

Objective To determine the prevalence, cause and distributions of blindness and poor vision in patients with leprosy.Methods An epidemiological survey of blindness and poor vision among 1045 cases of leprosy was carried out in Taixing City of Jiangsu Province, China.Results The prevalence of bilateral blindness was 7. 67%, unilateral blindness 4. 4%, bilateral poor vision of various degrees 9.28% and unilateral poor vision 5.84%. The prevalence of eye complications varied significantly among different groups of patients; females had a higher prevalence than males, multibacillary patients higher than paucibacillary patients, and in-patients higher than outpatients. Corneal disease was the most common cause of blindness in study groups, followed by iritic disease and cataract; while the main cause of poor vision was cataract, then corneal and iritic diseases. Treatable blindness accounted for 62. 7% of the cases and treatable poor vision for 88. 6% of the patients studied. 56. 62% of cases with eye complications expressed their willingness to be treated.Conclusions Although prevention and treatment of low vision and blindness in leprosy patients is very hard, it is necessary for doctors and medical workers to make clear of the factors to cause low vision and blindness, especially those in leprosy patients so that some measures for prevention and treatment of the disease could be taken accordingly.     

海口市2019年登革热本地和输入病例流行病学特征比较

目的 对2019年海口市登革热流行病学特征进行分析,比较本地和输入疫情特点,为海口市登革热防控工作提供参考依据。方法 根据“中国疾病预防控制信息系统”和海口市、区疾控中心流行病学调查资料,对登革热病例的流行特征、感染来源和病原学等进行描述性分析。结果 2019年累计报告登革热病例291例,发病率为12.64/10万。其中本地病例共报告251例（占86.3%）,输入病例共报告40例（占13.7%）。输入病例中境外输入病例32例,均来自东南亚柬埔寨和泰国等国家,国内输入病例8例,主要来自广西和云南。输入病例集中在5—11月,本地病例集中在8—11月;本地病例40~<50岁人群占29.5%,民工（47.4%）所占比例最高,其次为商业服务（9.6%）和工人（8.4%）;输入病例30~<40岁人群占40.0%,以商业服务（47.5%）、其他职业（12.5%）和工人（10.0%）为主;本地病例主要发生在建筑工地和城中村,而输入病例则集中在城区和城中村;病例从发病到诊断中位数为3.29 d,其中本地病例中位数为2.75 d,输入病例中位数为3.71 d;本地和输入病例就诊前自行购药的比例分别是7.6%和27.5%;77.3%的本地病例初次就诊选择省级或卫生院/社区及以下医疗机构,82.4%的输入病例初次就诊选择省级或市区级医疗机构;本地确诊病例均为登革病毒Ⅰ型,而输入病例中Ⅰ、Ⅱ、Ⅲ和Ⅳ型均有报告。结论 2019年海口市登革热疫情以输入病例引起本地暴发为主,本地和输入病例的流行病学特征差异明显,应根据本地和输入病例流行病学特点,制定相应的登革热防控策略。     

海口市2010—2017年疟疾病例流行病学特征分析

目的 分析海口市2010—2017年疟疾病例流行病学特征,为制订消除疟疾后的防控策略和措施提供科学依据。方法 采用描述性方法,对海口市2010—2017年报告的疟疾病例进行分析。结果 海口市2010—2017年报告疟疾病例36例,年发病率（0.09~0.31）/10万,死亡1例。病例全部为输入性,其中非洲输入27例,南亚3例,东南亚6例,海口市各区均有病例分布。1、4、5、7月发病占63.89%。病例分型恶性疟28例,间日疟6例,卵型疟2例。25.00%病例有典型疟疾临床症状,患者发病到就诊时间中位数为2 d,就诊到确诊时间中位数为1 d。病例治疗规范用药率为22.22% 。结论 海口市2010—2017年疟疾病例均为输入性,病例主要来自赴非洲务工后返乡的青壮年人群,节日前后发病较多,出现典型临床症状病例少,治疗规范用药率低。提高医疗机构特别是基层机构医务人员对疟疾的敏感性,加强输入性病例监测和规范病例治疗是今后工作重点。     

广西南宁市2008—2017年输入性登革热流行特征

目的 分析南宁市2008—2017年输入性登革热流行特征,为制定登革热防控策略提供参考依据。方法 对2008—2017年南宁市输入性登革热病例资料进行描述性流行病学分析。结果 南宁市2008—2017年共报告输入性登革热51例,无死亡,2009年和2014年的输入性病例较多,其余年度相对较少;病例集中于西乡塘（23例）、兴宁（10例）和青秀（9例）等3个主城区（共占82.35%）;输入高峰在9—10月,病例年龄中位数为23岁,以21~40岁青壮年为主（占82.35%）,职业多为学生、商业服务和干部职员;输入来源主要是东南亚（40例,占境外输入病例的97.56%）,其次是广东省（10例）;共发生输入性登革热暴发疫情3起;病例发病至诊断间隔时间的中位数为5 d。结论 应加强医务人员登革热诊疗知识培训,增强对询问流行病学史重要性的认识和提高监测灵敏性;加强重点人群的健康宣教,提高海关检疫和医疗机构早期诊断和报告的能力;加强蚊媒监测和控制工作。     

南宁市江南区2019年登革热流行病学特征和疫情应急处置分析

目的 分析总结南宁市江南区2019年登革热流行病学特征和疫情应急处置的工作情况,为今后有效地防制登革热提供对策、参考和技术支持。方法 收集南宁市江南区2019年登革热疫情相关数据,评价本次应急处置的工作成效。结果 2019年南宁市江南区登革热疫情严峻,共报告登革热病例370例,其中输入病例4例,本地病例366例;感染人数以家务待业和离退休者居多;男女性别比为1∶1.12;发病年龄最小1岁,最大92岁;发病的空间分布呈现高度聚集,福建园街道占本城区本地病例的87.70%。早期伊蚊应急监测布雷图指数和账诱指数合格率偏低,分别为72.17%和62.61%。针对本次疫情特性,制定有针对性的防控策略,做好精准疫情应急处置,有效压低峰值,迅速控制了疫情的扩散和蔓延。结论 本次疫情是由输入性病例导致本地病例社区水平暴发,疫情呈现多点暴发及扩散蔓延态势。需做好疫情研判、预警预测,准确分析流行病学特征,尽早实施登革热应急处置,精准防控、孳生地处理、健康宣教和病例管理是应急处置的关键措施。     

四川省广元市2011—2020年自然疫源和虫媒传染病流行特征

目的 分析2011—2020年四川省广元市甲乙类传染病中自然疫源及虫媒传染病的流行病学特征,为下一步精准科学防控该类疾病提供建议。方法 收集整理2011—2020年广元市甲乙类自然疫源及虫媒传染病的监测数据,运用描述性流行病学方法对其流行病学特征进行分析。结果 2011—2020年广元市共报告甲乙类自然疫源及虫媒传染病7种209例,年均报告发病率为0.81/10万;报告死亡13例,病死率为6.22%,年均报告死亡率为0.05/10万。发病趋势总体平稳。报告发病数由高到低排序依次为疟疾、流行性乙型脑炎、布鲁菌病、流行性出血热、狂犬病、登革热、钩体病。每年7—8月为发病高峰,7个县（区）均有病例发生,各县区发病率差异无统计学意义（χ²=11.30,P=0.07）。男性发病率明显高于女性（χ²=48.23,P<0.01）。30~34岁年龄组发病率最高,为1.85/10万,职业以农民为主,占39.71%。结论 广元市自然疫源及虫媒传染病发病趋势总体平稳。发病主要以输入性疟疾和本地乙脑病例为主,各地各部门应加强对外出务工、旅游人员的防病知识宣传,同时加强病例和虫媒监测,早发现早报告早处置。进一步做好疫苗接种工作,搞好爱国卫生运动,加大虫媒孳生场所清理,尤其是在夏秋季节要采取防虫措施,从而有效防控该类疾病的发生发展。     

辽宁省2014—2018年登革热流行特征分析

目的 分析辽宁省近年来登革热疫情特点及流行规律,为制定预防控制措施提供依据。方法 收集国家疾病监测信息报告管理系统2014—2018年登革热基本信息,个案流行病学调查资料,用描述流行病学方法分析登革热的流行特征和流行因素。结果 2014—2018年辽宁省共报告登革热输入病例38例,无死亡;各月均有病例报告,主要集中在沈阳（占52.63%）,男女比例为1.38∶1,以20~<50岁的青壮年（占84.21%）为主,职业多为家务及待业、工人;输入来源主要是东南亚、南亚（25例,占65.79%）,其次是非洲（7例,占18.42%）;发病到诊断时间中位数7 d。结论 辽宁省登革热均为输入性病例,加强重点人群健康宣教,提高早期诊断和报告能力,尽快采取控制措施是防制登革热的关键。     

海口市2010—2020年孕产妇死亡病因分析

目的 探讨海口市孕产妇死亡原因,分析孕产妇死亡评审情况,为制定有效降低孕产妇死亡率的政策提供理论依据。方法 收集海口市2010—2020年孕产妇死亡监测报告和按十二格表法评审孕产妇死亡病例资料,分析海口市孕产妇死亡原因分布、死亡评审结论和影响死亡的相关因素。结果 2010—2020年海口市本地户籍活产数共为232 088例,死亡孕产妇31例,死亡孕产妇年龄17~43岁,平均（30.71±5.82）岁。死亡孕产妇年龄25~43岁18例（占58.1%）。高中或中专以下学历22例（占71.0%）。家庭人均年收入<8 000.0元22例（占71.0%）。孕期保健情况平均（5.12±3.47）次;前三位死亡原因分别为妊娠合并心血管疾病8例（占25.81%）,产科出血5例（占16.13%）,妊娠合并肝脏疾病3例（占9.68%）;对比2010—2015年与2016—2020年两个时期的孕产妇死亡率和评审结果,两时期孕产妇死亡率差异均无统计学意义 （P>0.05）;按十二格表法评审孕产妇死亡病例,20例可避免死亡,10例不可避免死亡,1例未评审。可避免死亡的前三位影响因素包括医疗保健机构的环节知识技能、个人和家庭以及居民团体的环节知识技能、医疗保健机构环节管理。结论 高龄、低文化水平和低家庭收入孕妇为海口市孕产妇死亡的主要原因。高危孕产妇预防工作应关口前移至婚前、孕前保健阶段,另是加强医疗机构人员医疗保健专业技术水平,以有效降低孕产妇死亡率。     

海口市2004—2020年突发公共卫生事件流行特征分析

目的 分析2004—2020年海口市突发公共卫生事件流行特征,为制定防控策略提供参考。方法 应用国家突发公共卫生事件监测网络,收集2004—2020年海口市突发公共卫生事件相关数据进行描述性统计分析。结果 2004—2020年海口市共报告突发公共卫生事件174起,发病5 297人,总罹患率为0.74%,罹患率呈现下降趋势（χ²_趋势=4 307.85,P<0.001）;共死亡11人,死因均为化学性中毒;时间分布呈现双峰型特征,报告高峰在3—5月和9月;74.14%的事件发生在城区;72.99%的事件发生在学校及托幼机构;传染病事件占78.16%,以呼吸道传染病为主（占73.53%）,其次是肠道传染病（占20.59%）和虫媒传染病（占5.88%）;食源性疾病占20.11%,以细菌性食源性疾病为主（占71.43%）,其次是食源性化学性中毒（占11.43%）、植物性毒素中毒（占8.57%）和未检出致病因子（占8.57%）;建筑工地罹患率较高（23.79%）;其他公共卫生事件罹患率为6.70%,高于传染病事件和食源性疾病;介入处置时间越长,罹患率越高（χ²_趋势=2 233.61,P<0.001）;报告时已有病例数>14例的罹患率较高（2.87%）;事件持续时间≤7.10 d的罹患率较高（1.21%）。结论 海口市突发公共卫生事件以传染病事件和食源性疾病为主,加强学校及托幼机构、建筑工地等场所的传染病防控和食品卫生管理,提高监测报告敏感性,及早介入处置,可减少突发公共卫生事件的发生和危害。     

重庆市万州区2019年登革热暴发疫情流行特征及相关因素

目的 探讨分析登革热的流行病学特征及其发生因素,为登革热疫情防控决策提供科学依据。方法 采用描述流行病学方法对重庆市万州区2019年登革热流行病学调查疫情资料进行分析,用Excel 2007软件作数据统计处理。结果 2019年万州区登革热疫情333例,其中输入性4例,本地感染329例,罹患率20.68/10万。登革热疫情波及万州城区11个街道和4个镇,其中城区328例,占98.49%,农村5例占1.51%。4例输入性登革热病例均为国内输入,其中云南输入2例、海南输入1例、台湾输入1例。本地感染病例中,除1例在重庆渝北区感染发病回万州区治疗外,其余328例均分布在万州城区的11个街道辖区。333例病例中,临床诊断病例250例,实验室检测确诊病例83例,均为登革病毒血清型1型。时间分布为8月2例,9月288例,10月42例,11月1例,病例高峰出现在9月。328例本地感染病例分布在万州主城区11个街道,农村5例。发病数由多到少的前5位镇乡街道依次为钟鼓楼街道155例,牌楼街道93例,高笋塘街道29例,太白街道16例,陈家坝街道11例。患者年龄最小者11个月,最大者82岁,患者平均年龄为37.49岁。结论 近年登革热疫情呈上升态势,我地开通柬埔寨、泰国航班,加速人员流动,万州区白纹伊蚊密度较高,引起登革热暴发疫情,提示应加强登革热疫情和蚊媒监测,及时预警,为登革热防控决策提供科学依据。     

深圳市2015年首例输入性登革热病毒溯源分析

目的 对2015年深圳市报告的首例输入性登革热病例进行溯源分析。方法 收集该病例流行病学资料及血清样本,并通过免疫层析法和实时荧光RT-PCR对该病例血清中的特异性IgM抗体、IgG抗体、NS1抗原及病毒核酸进行检测。用C6/36细胞对血清进行病毒分离。对分离株的E基因进行序列测定,与不同型别的标准株及不同地区的分离株进行同源性分析并构建系统发生树。同时对糖基化位点、毒力位点上的序列进行比较。结果 从该病例血清中检测出IgM抗体、NS1抗原和2型登革病毒RNA,并成功从血清样本中分离出登革病毒DEN2-SZ1503。 SZ1503与标准2型登革病毒NGC株E基因的核苷酸及氨基酸序列同源性分别为93.8％和97.8％。系统发生树表明SZ1503与SG（EHI）D2 / 06572Y13株（Malaysia 2013）,具有最高相似性,且位于系统发育树的同一分支中。该分离株同1051株（Indonesia 76）、10株（Somalia 84）和271-206株（Sri Lanka 90）一起属于基因型Ⅳ。SZ1503株E基因的糖基化位点与其他登革热2型毒株相同,毒力位点也与之前报道的毒株一致。结论 病毒学、血清学和流行病学结果表明,该输入登革热病例是由2型登革热病毒引起的,SZ1503病毒株的遗传特征与马来西亚流行的登革热病毒一致。