桂枝茯苓胶囊抑制小鼠离体子宫收缩效应及其物质基础评价

目的 分析评价桂枝茯苓胶囊及各分离部位对小鼠离体子宫痛经模型收缩的拮抗效应,并探讨物质基础与效应之间的关系。方法 采用萃取法分离制备各部位样品;采用小鼠离体子宫收缩模型评价该方及各部位的生物效应;采用 LC-UV-MS-MS 法对具有显著活性的醋酸乙酯部位的主要色谱峰进行分析鉴定。结果 桂枝茯苓胶囊拮抗子宫平滑肌收缩的活性部位主要为醋酸乙酯部位,通过与对照品对照及质谱特征对醋酸乙酯部位色谱峰进行了归属和指认,鉴定了其中8个化合物分别为没食子酸、苦杏仁苷、芍药内酯苷、芍药苷、1,2,3,4,6-O-没食子酰葡萄糖、苯甲酸、苯甲酰芍药苷、丹皮酚。结论 桂枝茯苓胶囊中醋酸乙酯部位是治疗痛经的有效部位,为进一步研究桂枝茯苓胶囊的效应物质基础及建立以有效成分为基础的质量标准提供了依据。     

茯苓三萜成分RP-HPLC-ELSD指纹图谱研究

目的 利用RP-HPLC-ELSD技术建立不同菌株茯苓三萜的指纹图谱,为评价不同菌株培育茯苓药材的质量品质提供理论依据,以期规范培育茯苓的菌株,提高茯苓资源品质。方法 色谱柱为Kromasil 100-5 C₁₈柱（250 mm×4.6 mm,5 μm）;流动相为0.2%甲酸水溶液（A）-乙腈（B）,采用梯度洗脱法;体积流量为1.0 mL/min;柱温为30 ℃;蒸发光检测条件：雾化温度为40 ℃,压力为350 kPa,gain值为7;进样量为20 μL。结果 建立的指纹图谱中有18个共有峰,且方法学考察符合规定的标准。结论 该方法简便快速、准确可靠,可作为评价不同菌株茯苓药材质量的有效手段。     

强肝胶囊HPLC指纹图谱研究

目的 建立强肝胶囊HPLC指纹图谱并对其进行化学模式识别。方法 采用Diamonsil C₁₈柱（250 mm×4.6 mm,5 μm）,在230 nm波长下,以乙腈-0.05%磷酸水溶液进行梯度洗脱,测定了44批强肝胶囊样品;应用相似度分析软件建立强肝胶囊指纹图谱的共有模式,并对色谱峰进行了指认。结果 建立强肝胶囊HPLC指纹图谱,在确定的方法下,得到44批强肝胶囊的色谱图,并获得了44批样品的相似度;确定共有峰14个,其中13个归属到各药材,11个已确认成分。结论 强肝胶囊的HPLC指纹图谱的构建和共有色谱峰成分的确定为强肝胶囊药品质量控制提供参考。     

益胃胶囊中芍药苷的HPLC法测定

目的 建立益胃胶囊中芍药苷的测定方法。方法 采用HPLC法,Agilent Extend C₁₈色谱柱(250 mm×4.6 mm,5 μm);乙腈-0.01 mol/L磷酸溶液(13∶87)为流动相;检测波长为230 nm;柱温30 ℃;体积流量1 mL/min。结果 芍药苷在进样量0.162~0.486 μg与峰面积有良好的线性关系(r=1.000 0),平均回收率为99.4%,RSD为1.8%(n=6)。结论 建立的测定方法可作为控制益胃胶囊质量的参考依据。     

痛安注射液HPLC指纹图谱的研究

目的 建立痛安注射液的指纹图谱,更有效地控制该产品的质量。方法 采用HPLC法对痛安注射液指纹图谱进行研究。色谱柱为Diamonsil C₁₈柱(250 mm×4.6 mm, 5 μm),保护柱：Phenomenex C₁₈ (4 mm×3 mm, 5 μm);流动相：0.02 mol/L磷酸二氢钾-乙腈,线性梯度洗脱;体积流量：1 mL/min;进样量：10 μL;柱温：30 ℃;检测波长为230 nm。采用国家药典委员会推荐的中药色谱指纹图谱相似度评价系统的操作规范软件（版本2004A）进行评价。结果 制剂中选定的共有峰分离良好。结论 该方法为痛安注射液的质量控制提供了较全面的信息,利于产品的质量控制。     

广东土牛膝HPLC指纹图谱的研究

目的 采用HPLC法对广东土牛膝进行指纹图谱研究,为广东土牛膝药材质量控制提供依据。方法 实验采用Kromasil C₁₈柱(250 mm×4.6 mm,5 μm),以乙腈-水为流动相梯度洗脱,体积流量1.0 mL/min,柱温25 ℃,检测波长230 nm。结果 建立广东土牛膝药材HPLC指纹图谱,确定13个共有峰,确定其相对保留时间和相对峰面积。结论 在选定的条件下所建立的指纹图谱为广东土牛膝药材质量控制提供依据。     

凉粉草的特征指纹图谱研究

目的 建立凉粉草特征指纹图谱,为凉粉草的质量控制提供依据。方法 采用HPLC法测定,色谱柱为Dikma Diamonsil（2）C₁₈柱（250 mm×4.6 mm,5 μm）;流动相为乙腈-2%甲酸,梯度洗脱;检测波长为320 nm;体积流量为0.8 mL/min;柱温为25 ℃。结果 建立了凉粉草的特征指纹图谱,34批次凉粉草确定10个共有峰。结论 该法准确可靠,重复性好,为凉粉草质量控制提供了依据。     

HPLC-ELSD法测定周络通胶囊中知母皂苷BII

目的 建立HPLC-ELSD法测定周络通胶囊中知母皂苷BII的方法。方法 采用ES AquaSep C₁₈（250 mm×4.6 mm,5 μm）色谱柱,以乙腈–水（24∶76）为流动相,体积流量1.0 mL/min;柱温30 ℃;ELSD检测器,漂移管温度为75 ℃,加热级别为66%,载气压力为172.4 kPa。结果 知母皂苷BII在0.62～7.79 μg呈良好的线性关系,平均回收率为98.75%,RSD 1.77%。结论 该方法简便快捷、准确可靠,为周络通胶囊的质量控制提供了依据。     

HPLC法测定养血当归胶囊中阿魏酸

目的 建立HPLC法测定养血当归胶囊中阿魏酸的方法。方法 采用DIKMA Diamonsil C₁₈（150 mm×4.6 mm,5 μm）柱,流动相为甲醇-1%醋酸溶液（30∶70）,体积流量1.0 mL/min,检测波长320 nm。结果 阿魏酸线性范围为4.02～20.24 μg/mL;平均回收率为96.67%,RSD为1.77%。结论 本方法定量准确、重复性好,可作为养血当归胶囊的质量控制方法。     

散结镇痛胶囊中皂苷类成分的指纹图谱研究

目的 建立散结镇痛胶囊中皂苷类成分的HPLC指纹图谱。方法 经水饱和正丁醇提取后,用氢氧化钠溶液萃取,采用D101大孔树脂富集制备供试品溶液。色谱条件为Waters Symmetry^@ C₁₈（250 mm×4.6 mm,5 μm）色谱柱;流动相为乙腈-水,梯度洗脱;检测波长203 nm;体积流量1.0 mL/min;柱温30 ℃。采用国家药典委员会出版的《中药色谱指纹图谱相似度评价》（2004年A版）软件,对11批不同批次的散结镇痛胶囊中皂苷类成分指纹图谱进行相似度计算。结果 11批散结镇痛胶囊指纹图谱中有9个共有峰,各峰分离度良好,各批次间共有峰的相对保留时间RSD均＜1.0%,样品间相似度均＞95%。结论 本方法具有良好的精密度、重现性、稳定性,各共有峰间分离度高,可用于散结镇痛胶囊质量综合评价。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…