安神补脑胶囊中淫羊藿苷含量测定方法的研究

目的:建立安神补脑胶囊中淫羊藿苷含量的测定方法。方法:采用高效液相色谱法,C_(18)柱(4.6 mm×250 mm,5μm);流动相:乙腈-0.1%三氟乙酸(30∶70);检测波长:270 nm;流速:1.0 m L/min。结果:淫羊藿苷在0.050 6~2.024 0μg范围内有良好的线性关系(r~2=0.999),平均回收率为96.4%,RSD=0.56%。结论:该方法准确、快速、简便,可用于安神补脑胶囊中淫羊藿苷的含量测定。     

RP-HPLC法测定更舒安颗粒中淫羊藿苷

目的:为控制新药更舒安颗粒的产品质量,建立其有效成分淫羊藿苷的定量方法。方法:淫羊藿苷的定量测定采用RP-HPLC法。色谱柱为ZORBAXSBC18(250mm×4.6mm,5μm),流动相为乙腈-水(30∶70),流速1.00mL/min;柱温为25℃;检测波长为270nm。结果:淫羊藿苷在测定条件下,平均回收率为101.18%,RSD=0.62%,回归方程为Y=20.121X-3.7077,r=1.0000;最低检出限度为0.12μg/mL,线性范围在1.22-24.48μg/mL;精密度试验淫羊藿苷峰面积RSD为0.58%。结论:本方法准确、简便、专属性强、灵敏度高,结果稳定,适用于更舒安颗粒的质量控制。     

HPLC法测定仙乐雄胶囊中淫羊藿苷的含量

目的:建立仙乐雄胶囊中淫羊藿苷含量的测定方法.方法:采用HPLC法,ODS C18(4.6 mm×250 mm,5μm),以乙腈-水(30:70)为流动相,流速1.0 ml/min,检测波长270 mm.结果:淫羊藿苷在(5.35-85.60)μg·ml-1的进样浓度范围内与其峰面积呈良好的线性关系(r=0.9998),平均回收率为98.06%(RSD=0.44%,n=6).结论:本方法操作简便、灵敏、准确,可用于仙乐雄胶囊的质量控制.     

HPLC测定前列舒乐胶囊中淫羊藿苷的含量

目的 建立HPLC测定淫羊藿中淫羊藿苷含量的方法.方法 采用BDS HYPERSIL C18色谱柱(4.6mm×25mm,5 μm);流动相:乙腈-水(30:70);检测波长:270nm;流速:1.0mL/min.结果 淫羊藿苷在0.506～2.024μg范围呈良好的线性关系,r=0.9996(n=5),平均回收率99.4%,RSD=1.08%(n=5).结论 该方法简便、准确、重现性好,可作为控制前列舒乐胶囊质量的方法.     

补肾强身片中淫羊藿苷的含量测定

目的建立补肾强身片中淫羊藿苷的含量测定方法。方法对国内有五个企业生产的十三个批次的补肾强身片,采用Agilent Eclipse Plus C_(18)色谱柱(4.6×250 mm,5μm),流动相是乙腈-水(30:70),流速1.0 m L/min,检测波长为270 nm,柱温35℃。结果淫羊藿苷回归方程为:Y=20722X+4.495,相关系数r=1(n=6)。淫羊藿苷的线性范围在0.048~0.96μg。结论本方法操作简便、准确、专属性强、稳定,可用于补肾强身片中淫羊藿苷的含量测定。     

HPLC测定补肾壮骨颗粒中淫羊藿苷的含量

目的:补肾壮骨颗粒为我院院内制剂,淫羊藿是方中君药.文中采用HPLC建立补肾壮骨颗粒中淫羊藿苷含量的测定方法. 方法:采用HPLC法,色谱柱:Agilent HC-18(150mm×4.6mm,5μm),柱温:30℃,以乙腈-水(30∶ 70)为流动相,流速为1.0ml/min,检测波长270nm,测定了3批补肾壮骨颗粒中淫羊藿苷的含量. 结果:在HPLC色谱中,淫羊藿苷可与其他成分分离,且在0.0962～0.6734μg范围内与峰面积呈良好的线性关系,r2=1(P<0.001),样品平均回收率为94.4%,RSD为1.83%(n=6). 结论:该法分离效果好,分析快速、准确,灵敏度高、重现性好,可用于补肾壮骨颗粒中淫羊藿苷的含量测定.     

高效液相色谱法测定化瘀通冠颗粒中淫羊藿苷的含量

目的:建立化瘀通冠颗粒中主要有效成分淫羊藿苷的高效液相含量测定方法.方法:采用高效液相色谱法,色谱柱为Kromasil C18柱(4.6 mm×250 mm,5 μm),流动相为乙腈-水(26:74),流速为1.0 ml/min,检测波长为270 nm,柱温为室温.结果:淫羊藿苷进样量在0.019 49～0.389 80 μg范围内线性关系良好(r=0.999 8),平均回收率为100.03%.RSD为0.92%.结论:本方法简便可行、重复性好,可用于本制剂中有效成分淫羊藿苷的含量测定.     

复方仙灵脾颗粒质量标准研究

目的:建立复方仙灵脾颗粒的质量控制方法。方法:采用TLC法对方中淫羊藿、枸杞子进行鉴别;采用HPLC法测定样品中淫羊藿苷的含量。色谱条件:色谱柱为Welchrom C18柱(200 mm×4.6 mm,5μm);流动相:乙腈-水(25∶75);流速为1.0 m L/min;检测波长:270 nm;柱温:30℃。结果:薄层定性鉴别的斑点清晰,分离效果良好;淫羊藿苷含量在3~160μg/m L范围内线性关系良好(r=0.9998),平均加样回收率为100.20%,RSD为2.55%(n=6)。结论:本方法结果准确、操作简单,重复性良好,适合作为该制剂的质量控制标准。     

HPLC法测定益肾灵颗粒中淫羊藿苷的含量

目的 建立测定益肾灵颗粒中有效成分淫羊藿苷含量的方法.方法 采取高效液相色谱法测定,经乙腈一水为流动相,检测波长为270mm,柱温为25℃.结果 淫羊藿苷在2.8-28.0ug/ml(r=0.9999)范围内呈良好线性关系,淫羊藿苷的平均回收率为98.11%(RSD=0.73%,n=5).结论 方便简便,分离效果好,结果准确可靠,可以用于检测益肾灵颗粒的质量.     

RP-HPLC法同时测定明目颗粒中淫羊藿苷和仙茅苷含量

目的:采用反相高效液相色谱法同时测定明目颗粒中淫羊藿苷和仙茅苷的含量。方法:采用C18色谱柱(4.6 mm×250 mm,5μm),以甲醇(A)-1.2%冰乙酸(B)为流动相进行梯度洗脱。梯度洗脱程序为:0~10 min,8%~15%A;10~15 min,15%~25%A;15~25 min,25%~30%A;25 min~50 min,30%A。检测波长为283 nm,柱温30℃,流速1.0 m L/min,进样量:10μL。结果:淫羊藿苷在进样量为0.02~0.18μg范围内线性关系良好,Y=0.595X+1.17,r=0.999 5;仙茅苷在进样量为0.04~0.36μg范围内线性关系良好,Y=0.614X+2.737,r=0.999 5。淫羊藿苷和仙茅苷平均加样回收率符合含量测定的要求。结论:该方法准确、简单、灵敏,可用于明目颗粒中淫羊藿苷和仙茅苷的含量测定。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…