覆盆子药材的HPLC特征指纹图谱研究

目的:采用HPLC法建立覆盆子药材的指纹图谱。方法:应用Hypersil ODS C18色谱柱、1%冰醋酸水溶液和甲醇采用梯度洗脱,流速1ml/min,检测波长266nm。结果:精密度和重现性试验中各共有峰相对面积的RSD均小于5%,符合有关规定。结论:HPLC特征指纹图谱可作为控制覆盆子药材内在质量的标准。     

刺五加HPLC/UV/MS指纹图谱研究

目的 :采用HPLC/UV/MS法分别对刺五加药材水溶性和脂溶性成分进行指纹图谱研究 ,并对其主要成分进行定性。方法 :采用岛津ODSC18(4 6mm× 15 0mm ,5 μm)柱和AgilentZORBAXSB C18柱 (2 5 0mm× 4 6mm ,5 μm) ,分别用 0 5 %冰醋酸乙腈、0 5 %冰醋酸、乙腈、0 1%甲酸为流动相梯度洗脱 ,流速 :1ml/min。 结果 :得到分离度和重现性均较好的刺五加药材HPLC/UV及HPLC/MS指纹图谱 ,并分别对水溶性成分指纹图谱中 5个色谱峰和脂溶性成分指纹图谱中 8个色谱峰进行了初步定性。结论 :本方法可用于刺五加药材的指纹图谱测定 ,并为其质量控制提供参考。     

高效液相色谱法测定5种不同产地丹参中丹参素和原儿茶醛含量

目的 :测定 5种产地丹参中丹参素、原儿茶醛的含量。方法 :采用高效液相色谱法 ,Kro masil分析柱 (2 5 0mm× 4 6mm ,5 μm ;流动相 :甲醇 10g/L冰醋酸 2 0 80 ;紫外检测波长 :2 80nm ;流速 :1.0ml/min。结果 :丹参素在 4 .2～ 33.6 μg/ml,原儿茶醛在 0 .36～ 1.80 μg/ml范围内线性关系良好 ,丹参素和原儿茶醛最低检测限分别为 10 .5ng和 0 .6ng ,回收率分别为 99.36 % (RSD为 1.2 6 % )和 97.5 4% (RSD为 0 .81% )。结论 :该方法简便、灵敏、准确 ,样品处理简便易行 ,在考察评价丹参药材质量上有实用价值。不同产地的丹参 ,其丹参素和原儿茶醛含量有一定差异。     

高效液相色谱法测定丹参药材中丹参素的含量

目的：采用高效液相色谱法测定丹参药材中丹参素的含量。方法：以甲醇-0．5％冰醋酸(1：10)作为流动相，流速为1．0ml／min。结果：丹参中丹参素的含量为0．18％，回收率101．83％，RSD为0．176％。结论：此法可作为丹参和复方丹参制剂质量控制的标准。     

天麻药材HPLC指纹图谱研究

目的:研究天麻药材的HPLC 指纹图谱,为天麻药材的质量控制提供可靠方法.方法:采用HPLC 法.色谱柱为Ultimate XB-C18 柱(250mm×4.6mm,5μm),流动相为甲醇-1% 冰醋酸水溶液,梯度洗脱,柱温为30℃,流速1ml·min-1,检测波长270nm.结果:初步建立了天麻药材的HPLC 指纹图谱,标定出16 个共有峰,天麻素的加样加收率为102.66%,RSD<2.5%.结论:HPLC 指纹图谱可为天麻药材质量控制提供一种新模式,并可为未知天麻样品鉴别提供一个准确、快捷的工具.     

不同产地丹参药材指纹图谱比较研究

【目的】通过建立丹参药材的HPLC指纹图谱对不同产地丹参药材质量进行比较。【方法】采用C18柱,色谱柱为HypersilODS2(250 mm×4.6 mm,5μm);流动相为乙腈-0.5%冰乙酸溶液梯度洗脱;柱温为30℃;流速为1.0 ml/min;检测波长为270 nm;洗脱时间75 min;进样量20μl。【结果】用相似度软件对来自5个不同产地的10批药材指纹图谱进行分析,标定了8个共有峰,相似度评价均在0.9以上。【结论】通过丹参药材指纹图谱的比较,可为不同产地丹参药材遴选提供依据。     

黄芩指纹图谱的研究

目的：建立黄芩药材的HPLC指纹图谱,优化黄芩的提取方法和色谱条件,确定某些化学成分。方法：采用梯度洗脱方法进行色谱分离,AgilentTC-C18柱（4.6mm×250mm,5μm）,流动相：乙腈与0.1％冰醋酸溶液进行梯度洗脱,检测波长276nm,流速1.0ml／min,柱温25℃,使用标准品对其化学成分进行确定。结果：以水：乙醇：乙酸乙酯：冰醋酸（50：49：5：1）为提取溶剂,以乙腈与0.1％冰醋酸溶液进行梯度洗脱色谱峰分离效果较好并确定7种化学成分,优化了黄芩药材的HPLC指纹图谱。结论：本文建立的方法能较多的分离出色谱峰,可为黄芩药材的指纹图谱评价及物质基础的确定提供依据。     

丹参药材的综合质量评价研究

目的 建立丹参药材HPLC方法同时用于指纹图谱和多指标测定。方法 采用Diamonsil C₁₈柱（250 mm×4.6 mm,5 μm）,在280 nm波长下,以乙腈-0.05%磷酸水溶液梯度洗脱,测定了19批丹参药材的指纹图谱和丹参素、迷迭香酸、丹酚酸B及丹参酮Ⅱ_A 4个指标成分,对指纹图谱共有峰和相似度进行分析,并进行聚类分析和主成分分析。结果 在选定的色谱条件下,得到19批丹参药材的指纹图谱,标定了12个共有峰,并建立了丹参药材的指纹图谱共有模式,相似度在0.808～0.997。聚类分析结果将19批丹参药材分为两大类,与主成分分析结果及药材测定结果相一致。结论 不同产地丹参药材色谱峰的数目差别不大,但各指标成分量差别较大。该方法准确、可靠,为有效地评价丹参药材的综合质量提供了参考依据。     

RP-HPLC法测定枳实药材中橙皮苷含量

目的 :建立用反相高效液相色谱 (RP HPLC)测定枳实药材中橙皮苷含量的方法。方法 :采用色谱柱为HypersilODS2 5μmC1 8柱 (2 50mm× 4 6mm) ;流动相为乙腈 1 %冰醋酸水溶液 (2 0∶80 ) ;检测波长 :2 83nm ;参比波长 360nm ;流速 1 0mL/min。结果 :橙皮苷的线性范围分别为 0 40 7～ 3 2 56μg,橙皮苷的平均回收率为 1 0 1 5 %。结论 :RP HPLC测定枳实药材中橙皮苷含量的方法 ,不仅操作简便、准确 ,而且重现性好     

丹参及丹参注射液指纹图谱的HPLC-MS研究

目的 采用HPLC－UV－MS法对丹参药材、丹参注射液中间体及丹参注射液进行指纹图谱的研究。方法 采用Alltima C18分析柱，甲醇－水－冰醋酸梯度洗脱系统，流速：1mL/min，检测波长：281nm，MS册时记录总离子流（TIC）色谱图。结果 得到分离度较好的丹参药材、中间体及注射液的HPLC－UV及HPLC－MS指纹图谱。结论为丹参药材、中间体及注射液的质量控制提供全面、可靠的依据。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.