HPLC法测定田基黄药材槲皮素的含量

目的：建立田基黄药材中槲皮素的含量测定方法。方法：采用反相高效液相色谱法,迪马（钻石）C18色谱柱。甲醇-0.06%磷酸（52∶48）为流动相,流速为1.0mL.m in;检测波长360nm。结果：槲皮素的线性范围是7～42μg/m l（r=0.9999）;槲皮素平均回收率为100.3%,RSD=2.1%。结论：该方法简便、准确,可用于田基黄中槲皮素的含量测定。     

HPLC法测定消渴降糖胶囊中槲皮素的含量

目的：探讨用高效液相色谱法检测消渴降糖胶囊中槲皮素的含量。方法：应用高效液相色谱法,采用C18柱,以甲醇-0.4％磷酸（55：45）为流动相,以360nm为检测波长,检测消渴降糖胶囊中槲皮素的含量。结果：槲皮素峰与相邻色谱峰能有效分离,柱效较高,槲皮素对照品进样量在0．056～0．560μg范围内与峰面积呈良好的线性相关,回归方程为Y=40103．2X-561．4,相关系数r值为0．9998;精密度RSD=0．9％;10h稳定性试验RSD=1．10％;重复性试验结果显示。5份样品含量的变异系数RSD=1．30％;3个不同浓度的加样回收试验,平均回收率为98．7％,RSD=0．90％。结论：采用高效液相色谱法测定消渴降糖胶囊中槲皮素的含量,专属性强,结果可靠,重现性好,可用于消渴降糖胶囊中槲皮素的含量测定。     

HPLC法测定消渴降糖胶囊中槲皮素的含量

目的：用HPLC法测定消渴降糖胶囊中槲皮素的含量。方法：采用DiamonsillC18（5μm，200×4．6mm）色谱柱，流动相为甲醇-0．4％磷酸（53：47），检测波长：360nm。结果：槲皮素进样量在0．0880μg-0．7920μg范围内，与色谱峰面积呈良好线性关系，r=0．9997（n=5），平均回收率为98．61％，RSD为1．85％（n=5），精密度RSD为0．41％（n=5）。结论：HPLC法简便、灵敏、稳定，可用于槲皮素的含量测定。     

HPLC测定珍珠通络胶囊中丁香酚含量

目的：建立测定珍珠通络胶囊中丁香酚含量的HPLC方法。方法：用十八烷基硅烷键合硅胶为填充剂；甲醇-水（50：50）为流动相；检测波长215nm；进样量为10止。结果：通过方法学实验：丁香酚在（0．011-0．443）μg范围内呈良好的线性关系，得回归方程如下，Y=1479．3＋33441．4X（r=0．9999），平均回收率为98．3％，RSD=0．7％。结论：应用HPLC法可以测定珍珠通络胶囊中丁香酚含量。其测定结果可靠，方法准确、灵敏，重现性好。     

天龙咳喘灵中总黄酮和槲皮素含量测定简

目的：测定名医验方天龙咳喘灵复方制剂中总黄酮和槲皮素的含量。方法：采用紫外一可见分光光度法测定天龙咳喘灵中总黄酮的含量;RP—HPLC测定其槲皮素的含量。结果：分光光度法测定总黄酮时,其在0．008-0．048mg·mL-1范围内线性关系良好（r=1）,平均回收率为98．98％,RSD为1．65％;RP—HPLc测定槲皮素时,其在0．0168-4）．0840mg·mL-1范围内线性关系良好（r=O．9999）,平均回收率为99．83％,RSD为2．03％。结论：确定了复方天龙咳喘灵总黄酮和槲皮素的含量,建立了可靠、稳定易行并且重复性较好的测定方法,可作为该方剂的质量控制标准。     

HPLC法测定垂盆草颗粒中槲皮素的含量

目的：建立HPLC法测定垂盆草颗粒中槲皮素含量的方法。方法：采用RP—HPLC法,色谱柱为C18柱,流动相为甲醇-0．1％三氟乙酸溶液（46：54）,检测波长371nm,柱温30℃。结果：槲皮素在6．22μg／mL～62．24μg/mL范围内线性关系良好（r=0．9999）,平均回收率为98．0％（RSD=I．2％,n=6）。结论：本法操作简便、准确,可用于垂盆苹颗粒的质量控制。     

高效液相色谱法测定活血止痛胶囊的含量

目的：建立高效液相法测定活血止痛胶囊中阿魏酸含量的方法。方法：采用Agilent-C18（4．6mm×150mm,5μm）色谱柱：流动相：乙腈-0．085％磷酸溶液（17：83）;检测波长：316nm;流速：1．0ml／min。结果：替加氟在0．015-0．960μg范围内与峰面积呈良好的线性关系（r=-0．9999）,平均回收率为99．25％,RSD=0．30％（n=5）。结论：本方法简便、准确、重现性好．可用于活血止痛胶囊中阿魏酸的质量控制。     

维药老鼠瓜中槲皮素的含量测定及氨基酸的成分分析

目的：探讨测定维药老鼠瓜提取物中槲皮素的含量的方法及基本氨基酸的成分。方法：采用HPLC法测定老鼠瓜提取物中槲皮素的含量,确定老鼠瓜提取物中黄酮的含量。HPLC的色谱条件：色谱柱：Phenmenex Lnna5NC18（2）100色谱柱（250×4．60mm）,流动相：甲醇一磷酸（50：50）,流速0．07ml／min,柱温30℃,检测波长360nm。采用氨基酸自动分析仪对老鼠瓜中氨基酸进行分析测定。结果：对照品槲皮素的标准曲线为f（z）=6．1539e^007＋0．0019（r=0．999）,平均回收率为95．0％,RSD=0．3％。氨基酸测定除色氨酸没有测定外,总氨基酸含量为10．9%。结论：HPLC法简便快捷、精密度高、准确性好,可以作为维药老鼠瓜中槲皮素的含量测定方法。老鼠瓜除能药用外,还有较高的营养价值。     

活络健骨胶囊质量标准研究

目的：建立活络健骨胶囊的质量控制方法。方法：采用薄层色谱法对活络健骨胶囊中的白芍、青风藤进行定性鉴别，用HPLC法对其制剂中芍药苷进行含量测定。结果：活络健骨胶囊薄层色谱中斑点清晰，阴性对照无干扰；含量测定芍药苷线性范围为0．16-0．95μg（r=0．9997），平均回收率为98．9％（n=5），RSD为0．96％。结论：所建立的分析方法简便可行，可用于活络健骨胶囊的质量控制。     

HPLC法测定人参首乌胶囊中人参皂苷Rgl的含量

目的 建立人参首乌胶囊中人参皂苷Rg1的含量测定方法。方法 采用高效液相色谱（HPLC）法，固定相为kromasailCl8柱，流动相为乙腈-0．05％磷酸溶液（19：81），紫外检测波长203nm。结果 人参皂苷Rgl在0．0524-0．524mg／ml范围内线性关系良好r=0．9999（n=5），平均加样回收率为96．86％（n=9），RSD=1．19％。结论该方法简便、快捷、准确，可用于人参首乌胶囊中人参皂苷Rgl的含量测定。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.