海藻、甘草及其不同比例配伍后的水提取物的肝毒性研究

目的：评价海藻、甘草及其相配伍后的水提取物对肝脏毒性的影响。方法：制备离体肝灌流模型，分离出完整肝脏，先以Krebs-Henseleit等渗缓冲液冲洗肝脏，再以1640缓冲灌流冲洗灌流至流出液呈红色，将肝脏移至盛1640灌流液杯中，循环性前向性灌流，各剂量组药物分别缓慢注入盛1640灌流液杯中，定时收集灌流液供测AST、ALT用。结果：甘草水提取物在灌注液中低浓度时未观察到明显变化，高浓度60MINC RG出现毒性反应（P＜0．05）。海藻水提取物在灌流液中低浓度15min以后）都能导致肝细胞损伤，并与浓度和时间呈依赖关系。甘草：海藻（1：1）合煎液未见对肝细胞有损伤（P＞0．05），余下各比例合煎及单煎后混合液对肝细胞均有损伤，且随海藻含量增加毒性增加，合煎液毒性强于单煎合混合液。结论：海藻单煎液及除甘草：海藻（1：1）合煎液外各比例合煎及单煎后混合液均有一定肝毒性，而甘草单煎液在低浓度、短时间未见肝毒性。     

甘草与附子配伍后甘草黄酮含量变化的测定

目的:测定甘草与附子配伍后甘草黄酮含量的变化. 方法:采用高效液相色谱法测定甘草单煎液和甘草与附子合煎液中甘草苷的含量变化. 结果:甘草单煎液中甘草苷平均含量0.76%,甘草与附子合煎液中甘草苷平均含量0.48%. 结论:甘草与附子配伍后,甘草黄酮(甘草苷)含量明显减少,表明甘草能抑制附子的毒性.     

马钱子配伍减毒研究概述

从实验研究和临床应用两方面概述了马钱子配伍生地黄、甘草、赤芍、肉桂、麻黄、地龙、洋金花、黄连后对马钱子毒性的影响。参考文献21篇。     

甘草与乌头液对小鼠乌头毒量药动学参数的影响

目的探讨甘草与乌头合煎对小鼠毒量药动学参数的影响。方法将乌头煎液、甘草与乌头合煎液给小鼠腹腔注射,用小鼠急性死亡率法求得LD50和药动学参数。结果乌头煎液小鼠腹腔注射的LD50为5.27 g/kg,甘草与乌头合煎液小鼠腹腔注射的LD50为9.36 g/kg,乌头煎液、甘草与乌头合煎液小鼠腹腔注射体内均属一级动力学消除,呈一室开放模型,表观半衰期分别为1.18、0.92 h。结论甘草与乌头合煎对乌头小鼠毒量药动学参数均有不同程度的影响,能使其消除半衰期缩短,提示甘草与乌头合煎能加快乌头体内消除,可能降低乌头的毒性。     

不同炮制方法对马钱子中马钱子碱及士的宁的影响

目的探讨不同炮制方法对马钱子中马钱子碱及士的宁的影响。方法以油炸法、奶制法、烘烤法、甘草制法、砂烫法为主。结果奶制法中马钱子与士的宁的含量下降依次优于甘草制法、油炸法、烘烤法和砂烫法。另外在经过3次的验证试验得出马钱子在奶制法中的平均保留率为33.5%,士的宁平均保留率为26.4%。结论在马钱子炮制方法中,油炸法、奶制法、烘烤法、甘草制法、砂烫法均有各自的优缺点,但整体而言,奶制法炮制马钱子最佳,它不仅能有效降低毒性生物碱的含量,还能简单、快捷地测出马钱子中马钱子碱及士的宁的比例。     

中药洗浴治疗婴儿毒性红斑的护理

目的 探讨中药洗浴对婴儿毒性红斑的治疗效果.方法 对212例婴儿毒性红斑采用甘草、川连、银花煎永局部冲洗.结果 红斑均有不同程度地消退,显效75.47％.有效14.15％,总有效率达89.62％.结论 中草药洗浴治疗婴儿毒性红斑疗效好,使用方便.     

马钱子炮制品探讨

本文比较了马钱子不同炮制品性状的不同以及有效成分士的宁含量的差异。 马钱子为马钱科植物马钱Strychnos nuxVomica L.的干燥成熟种子。主产于印度、泰国、越南等国,在我国云南、广东、海南等地也有种植。其性寒、味苦、有通络、止痛、散结消肿的功效。主治风湿顽痹,麻木瘫痪,跌打损伤,痈疽肿痛,小儿麻痹后遗症,类风湿关节炎等。但在临床上毒性极大,用量为0. 3～0.6g。为了寻求一种有效的炮制方法,本文就是采用烘法、炸法、甘草水煮法之后,比较了马钱子中有效成分和毒性成分士的宁含量差别。     

附子与灸甘草和生甘草配伍其水煎液中乌头类生物碱溶出率的比较

对附子、附子与灸甘草、附子与生甘划的水煎液进行定量分析。其结果：附子与灸甘草同煎会子单煎其乌头类生物碱的溶出率降低２８．６８％；附子与生甘草同煎其溶出率降低８１．１８％。     

士的宁和马钱子碱及其氮氧化合物的毒性比较

士的宁和马钱子碱及其氮氧化合物的毒性比较马骋，何亚维，蔡宝昌，陈龙（中医药研究所２１００２９）主题词马钱子／分析，士的宁／毒性，马钱子碱／毒性ＳｔｒｙｃｈｎｉｎｅａｎｄＢｒｕｃｉｎｅＣｏｍｐａｒｅｄｗｉｔｈＳｔｒｙｃｈｎｉｎｅＮ－ｏｘｉｄｅａｎｄＢｒ...     

海藻甘草反药组合配伍对海藻玉壶汤中5种活性成分溶出含量影响

目的对甘草单煎液、海藻甘草配伍及于复方海藻玉壶汤中配伍后药效组分甘草苷、甘草酸、连翘苷、橙皮苷、阿魏酸的溶出含量进行测定并比较其差异,从物质基础层面,初步探讨海藻甘草反药组合的配伍规律及机制。方法制备甘草单煎液(GC)、海藻甘草合煎液(GH)、海藻玉壶汤煎液(HYD)、海藻玉壶汤去海藻煎液(HYD-H)、海藻玉壶汤去甘草煎液(HYD-G)、海藻玉壶汤去海藻甘草煎液(HYD-GH),采用HPLC法对各组煎液中5中成分进行了含量测定,计算各组煎液5种成分的溶出含量并进行比较。结果甘草苷、甘草酸溶出含量由高到低为HYD-HHYDGCGH;连翘苷、橙皮苷、阿魏酸溶出含量由高到低分别为:HYD-HHYD-GHHYD-GHYD。结论 HPLC方法简便、灵敏、准确、重复性好,适合用于研究海藻甘草配伍及于复方中配伍后活性成分溶出含量的变化规律。海藻与甘草这对反药组合单独配伍时,表现出引起甘草中甘草苷、甘草酸的溶出含量降低的结果。二者在复方中配伍应用时,甘草苷、甘草酸的溶出含量与甘草组基本持平。而与复方全方组相比,去掉海藻甘草反药组合的拆方组表现出引起甘草苷、甘草酸、连翘苷、橙皮苷、阿魏酸溶出含量升高的结果,可为海藻玉壶汤临床应用及反药组合能否配伍应用研究提供参考。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…