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超细化处理复方化毒膏、散抗炎及抑菌作用的研究 总被引:1,自引:0,他引:1
目的研究超细化复方化毒膏、散的抗炎及抑菌作用。方法应用大鼠足爪肿胀法造急性炎症模型,将动物模型分为三组,超细组(外敷超细化复方化毒膏)、传统组(外敷复方化毒膏)、模型组(外敷丹士林),检测动物模型的血流灌注量、血细胞浓度、血流速度、体表温度,测量足爪肿胀度。检测超细化复方化毒散醇提物和水提物对金黄色葡萄球菌、假单胞绿脓杆菌和埃希氏大肠杆菌的最小抑菌浓度。结果超细化复方化毒膏对动物模型局部微循环的影响:血流灌注量有明显的降低作用,为模型组的48.45%(P〈0.01),为传统组的66.42%(P〈0.05);对血细胞浓度有明显降低作用,为模型组的51.52%,与传统组比无显著性差异;对血流速度有明显增强作用,为模型组的64.24%(P〈0.05),为传统组的68.58(P〈0.05)。三组对体表温度的改善方面没有显著性差异。超细化复方化毒散的醇提物和水提物的最小抑菌浓度:对金黄色葡萄球菌分别为6.25、3.125mg生药/ml,对其余菌均为6.25生药mg/ml。结论超细化复方化毒散具有明显的抑菌作用,其膏的抗炎作用明显优于传统的复方化毒膏。 相似文献
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新鲜仙人掌和芦荟的抑菌实验研究 总被引:1,自引:0,他引:1
目的测定新鲜仙人掌和芦荟对金黄色葡萄球菌、铜绿假单胞菌等5种常见细菌的抑菌作用,以及二者在抑菌方面的差异。方法采用MH(B)营养肉汤递倍稀释法,分别研究二者在醇提与水煎两种方法下对五种常见细菌的最低抑菌浓度(MIC)。结果经醇提得到的两种药液,其抑菌效果明显高于水煎液,而仙人掌醇提液对几种常见细菌均有抑制作用,并且对金葡萄球菌的抑菌效果明显高于其它药液,芦荟醇提液对铜绿假单胞菌抑菌效果较明显。结论此实验结果可为临床治疗某些致病菌引起的感染时,选择仙人掌还是芦荟药物提供参考依据。 相似文献
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解毒宣透汤水提物和醇提物化学指标及药效的比较研究 总被引:3,自引:0,他引:3
目的:评价复方中药解毒宣透汤水提和醇提对化学成分指标含量以及对抗炎和抑菌实验结果的影响,考察化学成分指标与药效的相关性,探讨复方有效组分。方法:分别采用水提和醇提工艺制备解毒宣透汤提取物,用RP-LPLC法测定连翘苷、绿原酸和黄芩苷含量,并考察提取的抗炎和抑菌作用。结果:水提物和醇提物均具有抗炎和抑菌作用,且水提物的作用略强于醇提物,而水提物中连翘苷和黄芩苷含量低于醇提物,与复方的抗炎和抑菌作用呈非正性相关。结论:解毒宣透汤以水提为佳,复方的提取工艺筛选以药效为指标较妥。 相似文献
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5种中药醇提物体外抑制2种革兰阳性菌的活性 总被引:2,自引:2,他引:0
目的:研究丹参、丁香、厚朴、金银花、大黄这5种中药醇提物对痤疮丙酸杆菌和金黄色葡萄球菌的抑菌活力,进而通过正交试验考察不同配比对这2种革兰阳性菌的抑菌作用.方法:丹参、丁香、厚朴、金银花、大黄醇提物浓缩至0.2g·mL-1,通过测量抑菌圈直径考察不同中药醇提物与醇提物配伍后对这2种细菌的抑菌效果.结果:痤疮丙酸杆菌对丹参醇提物为高度敏感,抑菌圈直径为(23.27±3.68) mm,对丁香、厚朴醇提物为中度敏感,为(18.82±2.27),(17.78±1.88) mm;金黄色葡萄球菌对丁香和厚朴醇提物为高度敏感,抑菌圈直径分别为(21.94±0.74),(19.52士1.09)mm,对大黄和丹参为中度敏感,抑菌圈直径为(14.43±2.24),(16.01±0.88) mm.丹参、厚朴、丁香醇提物最佳复配比例为1∶1∶1,其对痤疮丙酸杆菌和金黄色葡萄球菌的抑菌圈直径分别为(20.07±1.26),(20.57±0.38) mm.结论:丹参、厚朴、丁香的醇提物对这2种菌的抑菌活力均为高度敏感,抑菌作用最佳.丹参、厚朴、丁香的醇提物对2种菌抑菌活性的最佳配比为1∶1∶1. 相似文献
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石香薷、苏州荠芋抑菌作用的实验研究 总被引:3,自引:0,他引:3
目的:比较石香薷、苏州荠芋水提物和醇提物的体外抑菌作用。方法:采用二倍稀释法测定提取物的最小抑菌浓度。结果:石香薷水提物抑菌作用显著强于醇提物;苏州荠荸水提物和醇提物的抑菌作用相近;苏州荠等对金黄色葡萄球菌的抑制效果较为显著,最小抑菌浓度为0.125g/ml。结论:苏州荠等水提物和醇提物与石香薷的水提物均具有一定的抑菌作用,苏州荠芋可进一步开发利用。 相似文献
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Harat ZN Sadeghi MR Sadeghipour HR Kamalinejad M Eshraghian MR 《Journal of ethnopharmacology》2008,115(1):36-41
AIM OF THE STUDY: Contraceptive plants which were introduced by folk in traditional remedies are investigated worldwide. In this study, the contraceptive effects of Ruta graveolens L., which has been mentioned for male contraceptive in Iranian traditional folk medicine, was experimented on human sperm. MATERIALS AND METHODS: Different doses of lyophilized aqueous extract of Ruta graveolens L. were added to an amount of fresh semen, containing 10(6) cells in a 1:1 volumic ratio. Motility and viability of cells, DNA status, mitochondrial activity and sperm revival tests were carried out. RESULTS: The sperm immobilization effects of the extract appeared immediately in a does-dependent manner and 100% of the sperms became immotile at a concentration of 100mg/ml but other parameters were intact. After washing the sperms, motility was returned in 30.8+/-3.2% of the sperms, besides coiled tails in 38.6+/-5.5% of the treated cells, in comparison to 12.5+/-2.0% of the control group (p=0.001). The part of the extract, responsible for immobilization of the sperms was stable upon boiling. CONCLUSIONS: As the cells were alive and immotile, probably some ionic currents are blocked by a thermostable component of the plant which can be promising as a new male channel blocker contraceptive. 相似文献
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复方芸香抗结核菌作用及其对Ⅲ型肺结核疗效 总被引:1,自引:1,他引:0
实验结果表明,复方芸香对试管内人型结核菌抑制作用较强,对牛型、鸟型结核菌则作用弱;对小鼠结核病模型疗效显著;对人结核病疗效也显著。 相似文献
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Ojala T Remes S Haansuu P Vuorela H Hiltunen R Haahtela K Vuorela P 《Journal of ethnopharmacology》2000,73(1-2):299-305
Antimicrobial screening against selected Gram-positive and Gram-negative bacteria, yeasts, mold, as well as plant pathogenic fungi, with emphasis on method optimization was carried out on methanol extracts prepared from seven plants grown in Finland. Sensitivity to the extracts was found to vary considerably among the micro-organisms, the extract from Petroselinum crispum and Ruta graveolens showing the highest toxicity against Rhizoctonia solani. The growth of Heterobasidium annosum was inhibited, whereas that of Phytophtora (cactorum) was promoted by all the extracts. The antibacterial and antifungal activities of six natural coumarin compounds were weak, except for the inhibitory effect against Fusarium culmorum. 相似文献
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The effects of Anethum graveolens L. (dill) extracts on the female reproductive system were studied in 54 Wistar female rats with regular estrous cycle in six groups. The experimental groups were fed with 0.045 g/kg and 0.45 g/kg of aqueous extract, 0.5 g/kg and 5 g/kg of ethanol extract for 10 days. The sham group was fed with solvent and the controls received no treatment. The estrous cycle changes were determined by daily vaginal smear changes. At the beginning and at the end of the experiment blood samples were provided to determine the blood estradiol and progesterone concentration. The ovaries were prepared histologically and the volume of different follicles was estimated. A significant increase was observed in the duration of the estrous cycle and in the diestrus phase and the progesterone concentration in high dose extract treatment. The stereological study did not reveal any significant changes in the volumes of ovaries, primary, secondary and graafian follicles. Dill can be used either as a regulatory agent of the menstrual cycle for women with irregular cycles or as an antifertility agent. More studies are needed to clarify the properties of this herb. 相似文献
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石榴皮中鞣质和黄酮类化合物抑菌作用的实验研究 总被引:17,自引:0,他引:17
目的探讨石榴皮中鞣质和黄酮类化合物的体外抑菌活性效果并确认其有效部位。方法提取两种化合物,采用试管两倍稀释法对金黄色葡萄球菌、福氏痢疾杆菌、沙门氏菌、大肠杆菌、绿脓杆菌和白色念珠菌进行体外抑菌实验。结果石榴皮中鞣质类化合物对金黄色葡萄球菌、福氏痢疾杆菌、沙门氏菌、大肠杆菌、绿脓杆菌和白色念珠菌均有不同程度的抑菌作用,而黄酮类化合物对绿脓杆菌和白色念珠菌抑菌效果不强。结论石榴皮中鞣质和黄酮类化合物有一定的抑菌作用,且鞣质类化合物呈现广谱抗菌特性,并具有抗耐药菌作用。 相似文献
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Pollio A De Natale A Appetiti E Aliotta G Touwaide A 《Journal of ethnopharmacology》2008,116(3):469-482
ETHNOPHARMACOLOGICAL RELEVANCE: Ruta is a genus of Rutaceae family. It features mainly shrubby plants, native to the Mediterranean region and present in traditional medicine of this region since Antiquity. The three most diffused species Ruta chalepensis L., Ruta graveolens L., and Ruta montana (L.) L., are morphologically poorly differentiated and were probably interchangeably used during Antiquity. AIM OF THE STUDY: Hippocratic and contemporary medical applications of the Ruta genus in the Mediterranean were compared to check if they result from a continuity of use from the ancient times to nowadays. RESULTS: Ruta spp. was mainly employed in medical preparations by Hippocratic physicians as an abortifacient and emmenagogue. In addition to gynaecological conditions, in several treatises of the Corpus HippocraticumRuta spp. were also recommended as a specific remedy against pulmonary diseases. Ruta spp. leaves and also roots and seeds, were administered for internal use by Hippocratic physicians after having been soaked in wine or mixed with honey or its derivatives. Contemporary traditional uses of Ruta spp. have been assessed in detail in the whole Mediterranean area. CONCLUSION: Nowadays, Ruta spp. is used to treat different conditions but, as a general rule, the external uses are preferred as the toxicity of the plant is widely acknowledged. 相似文献
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The recent approach of using herbs and antibiotics in combination constitutes a strategy to overcome the problems of resistance and side effects associated with conventional antibiotics. In the present study, the antimicrobial effect of Pelargonium graveolens L' Hér essential oil in combination with ciprofloxacin was evaluated on uropathogens, namely, Klebsiella pneumoniae KT2, Proteus mirabilis PRT3 and Staphylococcus aureus ST2. Minimum inhibitory concentrations of P. graveolens essential oil and ciprofloxacin were determined by the microbroth dilution method and further, the interaction between these two agents was studied by a checkerboard method. The fractional inhibitory concentration index (FICI) was calculated to be 0.375 for both K. pneumoniae KT2 and P. mirabilis PRT3, while for S. aureus ST2 it was found to be 0.5. The values of FICI for the tested microorganisms were found to be ≤0.5, which indicates synergism between P. graveolens essential oil and ciprofloxacin. The concave shaped curve in the isobolograms also depicted a synergistic effect of P. graveolens essential oil and ciprofloxacin against the tested microorganisms. Hence, the synergistic action of P. graveolens essential oil and ciprofloxacin may be applied for the treatment of UTIs, which have hitherto been treated by using only synthetic drugs. 相似文献