弯管花的化学成分

从茜草科植物弯管花树皮的甲醇提到物中分得５个化合物,经波谱技术鉴定为３,４－二羟基苯甲酸（Ｉ）,３－甲氧基－４－羟基苯甲酸（Ⅱ）３,５－二甲氧基－４－羟基苯甲酸（Ⅲ）２,６－二甲氧基对苯醌（Ⅳ）和６－甲氧基－７－羟基香豆素（Ｖ）,这些化合物为首次从该植物中得到。     

林茜草化学成分的研究

从林茜草Ｒｕｂｉｓ ｓｙｌｖａｔｉｃａ中首次分得１１个化合物,经理化常数和光谱分析,分别鉴定为５－羟基－萘并〔１－２－ｂ〕呋喃－４－羧酸甲酯（Ⅰ）,大叶茜草素（Ⅱ）、１－羟基－２－甲基蒽醌（Ⅲ）,β－谷甾醇（Ⅳ）,胡萝卜苷（Ⅴ）,１,３,６－三羟基－２－甲基－蒽醌－３－Ｏ－（６’－乙酰基）新橙皮糖苷（Ⅵ）,１,３,６－三羟基－２－甲基蒽醌－３－Ｏ新橙皮糖苷（Ⅶ）,萘酸双糖苷（Ⅷ）、１,３,６－三羟     

葫芦茶化学成分的研究(Ⅱ)

从葫芦茶Ｔａｄｅｈａｇｉ ｔｒｉｑｕｅｔｒｕｍ（Ｌ．）Ｏｈａｓｈｉ中又分得９个单体化合物。通过化学方法和小谱分析鉴定其结构分别为：乌素酸（Ⅰ）、冬青素Ａ（Ⅱ）、３,５－二羟基苯基－６－Ｏ－反式－对羟基－肉桂酰基－β－Ｄ－葡萄吡喃糖苷、山奈素－３－Ｏ－β－Ｄ－葡萄吡喃糖苷、山柰－３－Ｏ－β－Ｄ半乳吡喃糖苷（Ⅴ）、（＋）－儿茶素（Ⅵ）,３,５－二羟基苯基－β－Ｄ－葡萄吡喃糖苷（Ⅶ）,槲皮素－３－Ｏ－β     

灰苞蒿中物碱和黄酮成分研究

从灰苞蒿Ａｒｔｅｍｉｓｉａ ｒａｘｂｕｇｈｉａｎａ地上部分的混合溶剂（石油醚－乙醚－甲醇）提取物和乙醇提取物中首次分离并鉴定了１个生物碱和４个黄酮成分：Ｎ－苯基－２－萘胺，５，４＇－二羟基－３，６，７－三甲氧基黄酮（喷杜素），５，７－二羟基－３，３＇，４＇－三甲氧基黄酮（ｑｕｅｒｃｅｔｉｎ ３，３＇，４＇－三甲基醚）５，７－二羟基－６，３＇，４＇－三甲氧基黄酮（异泽兰黄素），５，７，４＇－三羟基－     

油丹化学成分的研究

从油丹Ａｌｓｅｏｄａｐｈｎｅ ｈａｉｎａｎｅｎｓｉｓ Ｍｅｒｒ,树皮中分离得到４个化合物,根据ＨＲ－ＳＩＭＳ、＾１ＨＮＭＲ、＾１３ＣＮＭＲ及二维核磁共振技术鉴定其结构分别为：一个木脂素ｅｕｓｉｄｅｒｉｎ Ａ「７Ｒ,８Ｒ）－３,４,５,３’－四甲氧基－△＾８’,９’－８－Ｏ－４‘,７－Ｏ－５’木脂素」（Ⅰ）;两个苄基异喹啉类生物碱（６,７－二甲氧基异喹啉基）－（４’－甲氧基苯基）甲酮（Ⅱ）、（６,７     

滇白珠化学成分的研究(Ⅰ)

从滇白珠Ｒｅｈｄ。根的氯仿提取物中分离得到１１个化合物，其中７个化合物经理化 波谱分析分别鉴定为东莨菪素（Ⅰ），棕榈酸（Ⅱ），胡萝卜苷（Ⅲ）（＋）－ｌｙｏｎｉｒｅｓｉｎｏｌ（Ⅳ）－甲氧基异落叶松树脂醇（Ⅴ），３，４，５－三甲氧基苯甲酸（Ⅵ）和乙酰丁香酸（Ⅶ）。化合物Ⅴ为一新天然产物，其它６个化合物为该属植物中首次报道。     

臭灵丹化学成分的再研究

 从臭灵丹［Ｌａｇｇｅｒａｐｌｅｒｏｄｏｎｔａ（ＤＣ．）Ｂｅｎｔｈ］地上部分的水煎浓缩液的氯仿、醋酸乙酯、正丁醇提取液中，通过反复硅胶柱层析及重结晶，分得４个化合物。经理化性质及光谱测定，分别鉴定为：臭灵丹二醇（ｐｔｅｒｏｄｏｎｄｉｏｌ）、臭灵丹三醇乙（ｐｔｅｒｏｄｏｎｔｒｉｏｌＢ）、３’，４’，５－三轻基－３，６，７．三甲氧基黄酮（３’，４’，５－ｔｒｉｈｙｄｒｏｘｙ－３，６，７－ｔｒｉｍｅｔｈｏｘｙｆｌａｖｏｎｅ）和橙皮甙（ｈｅｓｐｅｒｉｄｉｎ）。其中，后２个为首次从该植物中分离得到的化合物。     

柳穿鱼黄酮成分的研究

从柳穿鱼Ｌｉｎａｒｉａ ｖｕｌｇａｒｉｓ Ｍｉｌｌ．全草的乙醇提取物中分离到７个黄酮类化合物,经理化性质及光谱分析,分别鉴定为柳穿鱼苷元（Ｉ）,柳穿鱼苷（Ⅱ）,粗毛豚草素（Ⅲ）,刺槐素（Ⅳ）,乙酰蒙花苷（Ⅴ）,木犀草素（Ⅵ）和４＇－甲氧基－５,７,３＇－三羟基黄酮（Ⅶ）。其中Ⅲ,Ⅴ,Ⅵ和Ⅶ为首次从该属植物中得到。     

多成分综合评分法优化高良姜乙醇提取工艺研究

目的优化高良姜的乙醇提取工艺。方法建立高效液相色谱法,同时测定高良姜中5-羟基-7-(4-羟基-3-甲氧基苯基)-1-苯基-3-庚酮、高良姜素、山柰素、7-(4-羟基-3-甲氧基苯基)-1-苯基-4-烯-3-庚酮和1,7-二苯基-5-羟基-3-庚酮5种成分,采用L9(34)正交试验法,以上述5种成分含有量的综合评分为指标,考察乙醇体积分数、提取时间、提取次数和料液比4个影响因素,优选高良姜的醇提工艺。结果提取次数对高良姜的提取工艺有显著性影响,优选出的提取工艺为加10倍量80%乙醇,提取3次,每次1 h;高良姜中5种成分的转移率分别为5-羟基-7-(4-羟基-3-甲氧基苯基)-1-苯基-3-庚酮91.7%、高良姜素92.3%、山柰素85.0%、7-(4-羟基-3-甲氧基苯基)-1-苯基-4-烯-3-庚酮94.8%、1,7-二苯基-5-羟基-3-庚酮93.9%。结论多成分综合评分法优选出的提取工艺能对高良姜药材进行较完全地提取,可用于在线监控。     

马鹿茸化学成分的研究

从马鹿茸的乙醇提取物中首次分得１６个化合物。经理化常数和光谱分析，鉴定为：胆固醇肉豆蔻酸酯（Ｉ）、胆固醇油酸酯（Ⅱ）、胆固醇软酸酯（Ⅲ）、胆固醇硬脂酸脂（Ⅳ）、胆固醇（Ｖ）、胆旮－５０烯－３Ｂ－醇－７－酮（Ⅵ）、胆甾－５－烯－３β，７α－二醇（Ⅶ）、胆甾－５－烯－３β、７β－二醇（Ⅷ）、尿嘧啶（Ⅸ）、次黄嘌呤（Ⅹ）、同酐（Ⅺ）、脲（Ⅻ）、脲（ＸⅢ）、对羟基苯甲醛（ＸⅣ）、对羟基苯甲酸（ＸＶ）和尿苷     

高良姜根茎中一个新的二芳基庚烷

目的：对高良姜（Alpinia officinarum Hance）根茎化学成分进行研究。方法：运用多种层析方法进行分离纯化,通过波谱解析进行结构鉴定。结果：分离鉴定了5个二芳基庚烷类化合物,分别为：5-乙氧基-7-（4-羟基-3-甲氧基苯基）-1-苯基-3-庚酮（1）,5-羟基-1,7-二苯基-3-庚酮（2）,5-羟基-7-（4-羟基-3-甲氧基苯基）-1-苯基-3-庚酮（3）,5-甲氧基-7-（4-羟基-3-甲氧基苯基）-1-苯基-3-庚酮（4）,（E）-7-（4-羟基-3-甲氧基苯基）-1-苯基-4-烯-3-庚酮（5）。结论：化合物1为新的二芳基庚烷类化合物。     

元宝草化学成分的分离与鉴定

目的对元宝草进行化学成分研究。方法采用硅胶柱色谱、Sephadex LH-20柱色谱、重结晶等方法进行分离纯化,根据理化性质和波谱数据进行结构鉴定。结果分离得到9个化合物,分别鉴定为1,7-二羟基酮①、1,3,5,6-四羟基酮②、5,7,4’-三羟基-黄酮醇③、5,7,3’,4’-四羟基-黄酮醇④、金丝桃苷⑤、山萘酚-3-0-葡萄糖苷⑥、对羟基苯甲酸⑦、3,4-二羟基苯甲酸⑧、白桦脂酸⑨。结论化合物7,9为首次从该植物中分离得到。     

Antioxidative effects of curcumin and its analogues against the free‐radical‐induced peroxidation of linoleic acid in micelles

Curcumin (1,7‐bis(4‐hydroxy‐3‐methoxyphenyl)‐1,6‐heptadiene‐3,5‐dione, 1) is a yellow ingredient isolated from turmeric (curcumin longa). Many health benefits have been claimed for curcumin, and these have generally been ascribed to its radical‐trapping antioxidant properties. In order to find more active antioxidants with 1 as the lead compound, we synthesized curcumin analogues, i.e., 1,7‐bis(3,4‐dihydroxyphenyl)‐1,6‐heptadiene‐3,5‐dione (2), 1‐(3,4‐dihydroxyphenyl)‐7‐(4‐hydroxy‐3‐methoxyphenyl)‐1,6‐heptadiene‐3,5‐dione (3), 1‐(4‐hydroxy‐3‐methoxyphenyl)‐7‐(4‐hydroxyphenyl)‐1,6‐heptadiene‐3,5‐dione (4), 1,7‐bis (4‐hydroxyphenyl)‐1,6‐heptadiene‐3,5‐dione (5), 1,7‐bis(3,4‐dimethoxyphenyl)‐1,6‐heptadiene‐3,5‐dione (6), 1,7‐bis(4‐methoxyphenyl)‐1,6‐heptadiene‐3,5‐dione (7), and 1,7‐diphenyl‐1,6‐heptadiene‐3,5‐dione (8). Antioxidative effects of curcumin and these analogues against the peroxidation of linoleic acid were studied in sodium dodecyl sulfate (SDS) and cetyltrimethylammonium bromide (CTAB) micelles. The peroxidation was initiated thermally by a water‐soluble initiator 2,2′‐azobis(2‐amidinopropane hydrochloride) (AAPH), and reaction kinetics were monitored by the formation of linoleic acid hydroperoxides. Kinetic analysis of the antioxidation process demonstrates that these compounds, except 6, 7 and 8, are effective antioxidants in micelles by H‐atom abstraction from the phenolic groups. Compounds 2 and 3, which bear ortho‐diphenoxyl functionality, possess significantly higher antioxidant activity than curcumin and other analogues, and the 4‐hydroxy‐3‐methoxyphenyl group also plays an important role in the antioxidative activity. In addition, the synergistic antioxidant effect of these compounds with α‐tocopherol (vitamin E) in micelles was also studied by following the formation of linoleic acid hydroperoxides and the consumption of α‐tocopherol. It was found that these compounds could not synergistically interact with α‐tocopherol in micelles. Copyright © 2009 John Wiley & Sons, Ltd.     

Discovery of natural products from Curcuma longa that protect cells from beta-amyloid insult: a drug discovery effort against Alzheimer's disease

From Curcuma longa, two novel compounds, 4' '-(3' "-methoxy-4' "-hydroxyphenyl)-2' '-oxo-3' '-enebutanyl 3-(3'-methoxy-4'hydroxyphenyl)propenoate (calebin-A, 1) and 1,7-bis(4-hydroxy-3-methoxyphenyl)-1,4,6-heptatrien-3-one (2), and seven known compounds, 1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione (curcumin, 3), 1-(4-hydroxy-3-methoxyphenyl)-7-(4-hydroxyphenyl)-1,6-heptadiene-3,5-dione (demethoxycurcumin, 4), 1,7-bis(4-hydroxyphenyl)-1,6-heptadiene-3,5-dione (bisdemethoxycurcumin, 5), 1-hydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl)-6-heptene-3,5-dione (6), 1,7-bis(4-hydroxyphenyl)-1-heptene-3,5-dione (7), 1,7-bis(4-hydroxyphenyl)-1,4,6-heptatrien-3-one (8), and 1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadien-3-one (9), were isolated following a bioassay-guided fractionation scheme utilizing an assay to detect protection of PC12 cells from beta-amyloid insult. Compounds 1, 3-5, and 7 were found to more effectively protect PC12 cells from betaA insult (ED(50) = 0.5-10 microg/mL) than Congo red (10) (ED(50) = 37-39 microg/mL).     

Six new diarylheptanoids from the seeds of Alpinia blepharocalyx

Chromatographic separation of part of an EtOH extract of the seeds of Alpinia blepharocalyx resulted in the isolation of six new (1-6) and two known (7, 8) diarylheptanoids together with 12 known compounds. The structures of the new compounds, including their absolute stereochemistry, were elucidated by spectroscopic and chemical methods as (3S,5S)- (1) and (3S,5R)-3-hydroxy-5-methoxy-1-(4-hydroxyphenyl)-7-phenyl-6E-heptene (2), (3S,5S)- (3) and (3S,5R)-3-hydroxy-5-ethoxy-1-(4-hydroxyphenyl)-7-phenyl-6E-heptene (4), (3S)-methoxy-1,7-bis(4-hydroxyphenyl)-6E-hepten-5-one (5), and 1,7-bis(4-hydroxyphenyl)hepta-4E,6E-dien-3-one (6). Among the isolated compounds, 5, (3S,5S)-3,5-dihydroxy-1,7-bis(4-hydroxyphenyl)heptane (8), 4'-hydroxy-5,6-dehydrokawain (14), and/or phloroglucinol (20) showed significant antiproliferative activity against murine colon 26-L5 carcinoma (ED(50): 5, 5.2 microM; 8, 12.8 microM; 14, 20.7 microM; 20, 26.4 microM) and human HT-1080 fibrosarcoma (ED(50): 5, 10.1 microM; 14, 20.1 microM; 20, 20.9 microM) cells.     

Phenolic compounds from the roots of Lindera fruticosa

The phenolic compounds isolated from the roots of Lindera fruticosa included four new compounds, 2-methoxy-3,4-methylenedioxybenzophenone (1), (S)-2-methoxy-3,4-methylenedioxybenzhydryl alcohol (2), 3-hydroxy-5-methoxybiphenyl (3), and 1-methoxy-2,5,7-trihydroxyxanthone (4). Three previously identified phenolics were also identified, namely, 3,5-dimethoxybiphenyl (5), benzyl 2-hydroxy-6-methoxybenzoate (6), and 1,7-dihydroxyxanthone (7). These compounds were evaluated for their inhibitory effects on human acyl-CoA:cholesterol acyltransferase activity and on the in vitro oxidation of low-density lipoprotein.     

绵萆薢中两个新二芳基庚酮类化合物的分离和结构鉴定

目的：研究绵萆薢（Dioscorea spongiosa）的化学成分。方法：利用大孔吸附树脂、硅胶柱层析、制备薄层色谱法进行分离纯化，根据化合物的理化性质和光谱数据鉴定结构。结果：从绵萆薢中分离得到5个化合物，分别鉴定为（3R，5S，7S）-1，7-二苯基-3，7-环氧-5-羟基-1-庚酮（命名为绵萆薢素A，化合物1）、（3S，5S，7S）-1，7-二苯基-3，7-环氧-5-羟基-1-庚酮（命名为绵萆薢素B，化合物2）、（＋）-丁香树脂醇（化合物3）、胡椒醇（化合物4）、芝麻素酮（化合物5）。结论：化合物1、2为新化合物，化合物3、4和5是3个首次从薯蓣属分得的木脂素类化合物。     

Trypanocidal diarylheptanoids from Aframomum letestuianum

Three new diarylheptanoids, (4Z,6E)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-7-(4-hydroxyphenyl)hepta-4,6-dien-3-one, letestuianin A (1), (4Z,6E)-5-hydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-4,6-dien-3-one, letestuianin B (2), and 1,7-bis(4-hydroxyphenyl)heptan-3,5-dione, letestuianin C (3), as well as the known (4Z,6E)-5-hydroxy-1,7-bis(4-hydroxyphenyl)hepta-4,6-dien-3-one (5) were isolated from Aframomum letestuianum. The known flavonoids 3-acetoxy-5,7,4'-trihydroxyflavanone, 3-acetoxy-7-methoxy-5,4'-dihydroxyflavanone, 7-methoxy-3,5,4'-trihydroxyflavone, and 3,3',4',5,7-pentahydroxyflavan were also obtained from this plant. Their structures were determined using a combination of 1D and 2D NMR techniques. The four diarylheptanoids were tested for growth inhibitory activity in vitro versus bloodstream forms of African trypanosomes. IC(50) values in the range of 1-3 microg/mL were found for compounds 3 and 5.     

瑶药扁担藤化学成分的研究

 目的 研究我国瑶族植物药扁担藤[Tetrastigma planicaule(Hook.) Gagnep.]的化学成分。方法 采用色谱技术进行分离,根据化合物的理化性质和光谱数据鉴定其结构。结果 从扁担藤茎的体积分数95%乙醇提取物中分离得到8个化合物,分别鉴定为stigmast-4-en-6β-ol-3-one(1),7α-hydroxysitosterol(2),古柯二醇(3),水杨酸(4),香草酸(5),丁香酸(6),原儿茶酸(7),glycerol-2-(3-methoxy-4-hydroxybenzoic acid) ether(8)。结论 所有化合物均为首次从该植物中分离得到,其中化合物1~3,5~8为首次从该属植物中分得。化合物8为首次从天然产物中分离得到。     

Antioxidant activity of caffeoyl quinic acid derivatives from the roots of Dipsacus asper Wall

The methanol extract from Dipsacus asper Wall (Dipsacaceae) was found to show antioxidant activity against free radical and Cu(2+)-mediated LDL oxidation. In further study, to identify active constituents from the plant, six caffeoyl quinic acid derivatives: 3,4-di-O-caffeoylquinic acid (1), methyl 3,4-di-O-caffeoyl quinate (2), 3,5-di-O-caffeoylquinic acid (3), methyl 3,5-di-O-caffeoyl quinate (4), 4,5-di-O-caffeoylquinic acid (5) and methyl 4,5-di-O-caffeoyl quinate (6) were isolated. Their structures were identified by spectroscopic methods including 2D-NMR. The isolated compounds, 1-6, were found to be potent scavengers of the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), and are more potent than butylated hydroxyl toluene (BHT) used as a positive control. The compounds 1-6 also inhibited Cu(2+)-mediated low-density lipoprotein (LDL) oxidation. They increased the lag time of conjugated dienes formation and inhibited the generation of thiobarbituric acid reactive substances (TBARS) in a dose-dependent manner. These results suggested that Dipsacus asper due to its antioxidant constituents, 1-6, may have a role to play in preventing the development and progression of atherosclerotic disease.