中药复方双草退黄冲剂1号抗鸭乙型肝炎病毒作用的实验研究

目的：研究中药复方双草退黄冲剂１号（ＳＣＧ－１）对鸭乙型肝炎病毒的体内抗病毒作用。方法：以感染鸭乙型肝炎病毒（ＤＨＢＶ）的广州麻鸭为实验动物模型。１日龄广州麻鸭感染ＤＨＢＶ，１３天后血清ＤＨＢＶ－ＤＮＡ全部阳性，给予ＳＣＧ－１治疗１４天，于感染后第１３天（为用药前），用药７、１４天及停药后３天分别采血，采用斑点杂交方法检测血清ＤＨＢＶ－ＤＮＡ。结果：ＳＣＧ－１１０ｇ／（ｋｇ· ｄ）组，用药７、１４天鸭血清ＤＨＢＶ－ＤＮＡ　ＯＤ值均明显低于给药前，有显著性差异（Ｐ＜０．０５，Ｐ＜０．０１）；ＳＣＧ－１５ｇ／（ｋｇ．ｄ）组用药１４天血清ＤＨＢＶ－ＤＮＡ ＯＤ值亦明显低于用药前（Ｐ＜０．０５），但两组停药后３天均有反跳现象。结论：ＳＣＧ－１用药期间有一定的抑制ＤＨＢＶ－ＤＮＡ复制作用。     

定量PCR检测黄芪A6组分与无环鸟苷联合抗1型单纯疱疹病毒的协同作用

目的：探讨抗疱疹病毒药物黄芪Ａ６组分（Ａ６）和无环鸟苷（ＡＣＶ）联合抗１型单纯疱疹病毒（ＨＳＶ１）的作用机制。方法：利用竞争ＰＣＲ检测Ａ６和ＡＣＶ联合抗ＨＳＶ１的协同作用，并与细胞病变（ＣＰＥ０抑制法进行比较。结果：竞争ＰＣＲ测定Ａ６、ＡＣＶ和Ａ６＿ＡＣＶ对ＨＳＶ１的最小抑制浓度（ＭＩＣ）分别为１．８８ｍｇ／ｍｌ、３．３７μｇ／ｍｌ、３．３７μｇ／ｍｌ和０．４７ｍｇ／ｍｌ＋０．８４μｇ／ｍｌ；ＣＰ     

生命泉流浸膏抗人巨细胞病毒的体外实验研究

以病毒唑（ＲＢＶ）作阳性对照药,采用ＭＴＴ法和细胞病变（ＣＰＥ）抑法,观察生命泉流浸膏（ＦＥＳＯＬ）抗人巨细胞病毒（ＨＣＭＶ）的作用,结果ＭＴＴ法测得ＦＥＳＯＬ的ＴＣ５０为２．３８ｍｇ／ｍｌ,ＩＣ５０为０．５４７ｍｇ／ｍｌ,治疗指数ＴＩ为４．３５;ＣＰＥ抑制法测得ＦＥＳＯＬ和ＴＣ５０为３．２０ｍｇ／ｍｌ,ＩＣ５００．４０３ｍｇ／ｍｌ,治疗指数ＴＩ为５．４６。结论与阳性药ＲＢＶ相比,ＦＥＳＯＬ在体外     

中药复方双草退黄冲剂1与抗鸭乙型肝炎病毒作用的实？…

目的：研究中药复方双草退黄冲剂１号（ＳＣＧ－１）对鸭乙型肝炎病毒的体内抗病毒作用。方法：以感染甲乙型肝炎病毒（ＤＨＢＶ）的广州麻鸭为实验动物模型。１日龄广州麻鸭感染ＤＨＢＶ,１３天后血清ＤＨＢＶ－ＤＮＡ全部阳性给予ＳＣＧ－１治疗１４天,于感染后第１３天（为用药前）,用药７、１４天及停药后３天分别采血,采用斑点杂交方法检测血清ＤＨＢＶ－ＤＮＡ。结果：ＳＣＧ－１ １０ｇ／（ｋｇ．ｄ）组,用药７、１４天     

山地香茶菜对HIV—1作用的实验研究

为研究山地香茶菜提取物对艾滋病病毒１型（ＨＩＶ－１）有无抑制作用，按照以下ＭＴＴ法，进行了抗病毒实验研究。结果表明，山地香茶菜提取物Ｈ－１在０．６２５～２．５ｍｇ／ｍｌ剂量范围内，Ｈ－９在０．３１２５～０．６２５剂量范围内，对ＨＩＶ－１有一定抑制作用，其５０％有效浓度分别为０．５６６７ｍｇ／ｍｌ和０．２１５ｍｇ／ｍｌ。     

地锦草总黄酮抗氧化作用的研究

地锦草总黄酮呈剂量依赖性的抗Ｏ－２·和·ＯＨ－的氧化作用。在ＶＢ２－Ｍｅｔ－ＮＢＴ体系中对Ｏ－２的ＳＣ５０＝０．５８μｇ／ｍｌ，ＩＣ５０＝０．３５μｇ／ｍｌ；在Ｖｉｔ·Ｃ－Ｃｕ２＋－Ｃｙｔｏ·Ｃ体系中对·ＯＨ－的ＳＣ５０＝２．４μｇ／ｍｌ，ＩＣ５０＝２．１μｇ／ｍｌ；在Ｖｉｔ·Ｃ－Ｆｅ２＋－ＤＲ体系中对ＭＤＡ的ＳＣ５０＝１５１．８μｇ／ｍｌ。地锦草的消肿、清热，伤口愈合等药效作用的发挥，可能与其清除和抑制自由基有关     

润喉宁抗病毒作用的研究

润喉宁（由地黄、射干、乌梅、薄荷等组成）在０．０６８９ｇ／ｍｌ的浓度时，能明显抑制流感病毒ＦＭ１株、单纯疱疹病毒Ⅰ型（ＨＳＶ－１）和腺病毒Ａ_３所致的细胞病变（ＣＰＥ）。免疫荧光试验显示，当其浓度为０．１４ｇ／ｍｌ和０．２８ｇ／ｍｌ时，均能抑制流感病毒ＦＭ１和ＨＳＶ－１在Ｈｅｐ－２细胞上的增殖。以５，１０，２０ｇ／ｋｇ·ｄ剂量ｉｇ给予小鼠，对小鼠流感病毒性肺炎及鼠肺内流感病毒的增殖量、均有明显抑制作用。     

异丙酚用于全麻诱导时对血流动力学的影响

目的：研究异丙酚用于全麻诱导时对血流动力学的影响。方法： ＡＳＡⅠ～Ⅱ 级择期手术患者 ４０ 例，随机分为两组，第Ⅰ组硫喷妥钠用量为 ５ｍｇ／ｋｇ；第Ⅱ组异丙酚２．５ｍｇ／ｋｇ。用阻抗法观察ＭＡＰ，ＨＲ，ＣＯ， ＣＩ， ＳＶ， ＳＩ， ＴＦＩ， ＶＥＩ， ＳＶＲ的变化。结果．注药后 １０ｍｉｎ ＣＯ， ＣＩ， ＳＶ， ＳＩ等值第 Ⅰ 组下降 ２０％～３０％，第Ⅱ组下降 １０％～２０％。结论：异丙酚对血流动力学影响轻于硫喷妥钠。     

多剂量氟罗沙星在健康人体内的药代动力学研究

 对１０例健康志愿者单次及多次ｐｏ４００ｍｇ氟罗沙星后进行药代动力学研究，血药浓度及尿药浓度均采用微生物法和ＨＰＬＣ测定。单次及多次给药后的数据分别采用３Ｐ８７及ＳＳＤ程序处理，药－时曲线符合二室模型。单次ｐｏ４００ｍｇ氟罗沙星后，其微生物法和ＨＰＬＣ测定结果分别为Ｔ＿（ｍａｘ）２．２０ｈ和２．１３ｈ，ｃ＿（ｍａｘ）５．４９μｇ／ｍｌ和５．５５μｇ／ｍｌ，Ｔ＿（１／２β）１０．１４ｈ和１０．５５ｈ。０～４８ｈ尿药回收率分别为：５６．６７％和５２．２５％。经ｔ检验２种方法的测定结果无显著性差异（Ｐ＞０．０５）。在每日４００ｍｇ连续８ｄ的多次ｐｏ给药实验中，第１ｄ和第８ｄ的药代动力学参数经ｔ检验无显著性差异（Ｐ＞０．０５），说明本品每天１次的重复给药无明显蓄积现象。     

HPLC测定前列腺中依诺沙星含量及药动学研究

 采用高效液相色谱法，对前列腺中依诺沙星的测定和药动学进行了研究。固定相：Ｓｈｉｍａｄｚ－ＯＤＳ柱，流动相：甲醇－乙腈－０．１ｍｏｌ／Ｌ柠檬殴（４．５：１：９），检测器：ＳＰＤ－６ＡＶ，检测波长：２７２ｎｍ，流速：１．５ｍｌ／ｍｉｎ，标准曲线线性范围：０．５～５μｇ／ｍｌ，ｒ＝０．９９９６，平均回收率为９８．８％，ＲＳＤ为０．７％，用３Ｐ８７实用药代动力学程序进行数据处理，在前列腺中各药代动力学参数Ｔ＿（１／２Ｋｅ），Ｔ＿（ｐ，ｃ＿（ｍａｘ）），ＡＵＣ，Ｃｌ／Ｆ（ｓ）和Ｖｃ／Ｆ（ｃ）分别为２．２３ｈ，２．３７ｈ，７．４３μｇ／ｍｌ，３９．４７（ｈ·μｇ）／ｍｌ，１．２７Ｌ／（ｋｇ·ｈ）和４．０７Ｌ／ｋｇ，结果表明，依诺沙星很容易透渗到前列腺组织中去，前列腺药物浓度很高，为临床应用依诺沙星治疗前列腺炎提供了理论依据。     

6-羟多巴胺化学损毁小鼠外周交感神经对艾灸免疫调节作用的影响

应用 6 OHDA化学切除外周交感神经轴突纤维 ,观察艾灸对正常和CY(环磷酰胺 )免疫抑制小鼠免疫功能的影响。结果 :应用 6 OHDA及CY后 ,小鼠胸、脾指数及IL 1、IL 2含量均低于正常组水平 ;艾灸治疗能提高小鼠免疫功能 ,改善其免疫抑制 ,上述指标均高于各自对照组 ,但仍低于交感神经完整的艾灸组。结果表明 ,6 OHDA损毁外周交感神经末梢后 ,艾灸增强与调节免疫的作用被削弱或部分阻断 ,提示交感神经参与艾灸对免疫的调节。     

黄连素合用环孢素A对小鼠皮肤移植排斥反应的影响

目的观察黄连素合用环孢素A抗同种异体小鼠皮肤移植排斥反应的影响,探讨其作用机制。方法以BALB/c小鼠为供体,以C57BL/6小鼠为受体,采用背-背皮肤移植手术法制备模型。将受体小鼠按随机数字表法分为模型组、黄连素组、环孢素A组、联合组,每组20只;另取20只健康C57BL/6小鼠作为假手术组。造模后黄连素组小鼠腹腔注射黄连素100 mg/kg,环孢素A组小鼠腹腔注射环孢素A注射液10 mg/kg,联合组腹腔注射黄连素100 mg/kg及环孢素A注射液5 mg/kg,假手术组和模型组小鼠腹腔注射等体积0.5%羧甲基纤维素钠。1次/d,连续10 d。记录受体小鼠移植皮片的存活时间;采用ELISA法检测血浆Th1类细胞因子[IL-2、γ-干扰素(interferon-γ,IFN-γ)]和Th2类细胞因子(IL-4、IL-10)水平;采用流式细胞术检测脾脏T淋巴细胞亚群CD4+CD25+比例。结果与模型组比较,各给药组受体小鼠移植皮片的存活时间延长(P<0.01);联合组移植皮片存活时间较黄连素组或环孢素A组延长(P<0.01)。与模型组比较,联合组血浆IL-2[(11.55±3.14)pg/ml比(19.85±2.42)pg/ml]、IFN-γ[(26.41±6.20)pg/ml比(57.23±10.15)pg/ml]水平降低,IL-4[(192.45±70.12)pg/ml比(61.09±21.61)pg/ml]、IL-10[(106.79±27.83)pg/ml比(40.08±11.23)pg/ml]水平升高(P<0.05),脾脏CD4+CD25+调节性T细胞比例[(7.65±2.42)%比(3.69±0.83)%]升高(P<0.01)。结论黄连素合用环孢素A通过促使Thl向Th2细胞的偏移、诱导免疫耐受、刺激CD4+CD25+调节性T细胞的表达等途径,抑制同种异体小鼠的皮肤移植排斥反应。     

华蟾素局部注射加针灸围刺治疗眼睑带状疱疹

目的 观察华蟾素注射液局部注射加针灸围刺治疗带状疱疹的疗效.方法 将确诊为眼睑带状疱疹的患者(50例),用75％乙醇消毒病灶,用1寸的针灸针,针尖向病灶中心围刺,针尖距病灶皮肤边缘约3mm,体针取合谷穴,留针15 min,取针后,在眼睑、颜面、发际,取3个病变严重部位,注射华蟾素各1ml,每日1次,5次为1个疗程 结果 50例患者的皮肤疱疹均在用药后2～7 d消失,10d皮肤恢复正常.结论 华蟾素注射液局部注射加针灸围刺治疗眼睑带状疱疹疗效确切,值得在临床上推广应用.     

皮下针、点灸及刺络拔罐治疗带状疱疹

目的：观察皮下针围刺加点灸、刺络拔罐治疗带状疱疹镇痛及促进疱疹结痂的临床疗效。方法：治疗组给予治疗如下：①常规消毒皮损部位,在疱疹周围用1．5寸毫针围刺,留针30min,1次／日;②将直径3mm的麻绳用麻油煮沸干燥后备用,用时将麻绳一端点燃,吹灭明火,沿疱疹周围每隔5厘米点灸一壮;③以梅花针重扣皮损局部,以皮肤出血为度,然后拔罐,拔罐数根据皮损大小而定,将皮损拔满火罐为止,留罐5～10min,以拔出每罐拔出黑血2～3ml,局部皮肤充血发紫为佳。拔罐后局部皮肤水汪汪消毒。皮下针每日1次,刺络拔罐隔日1次。对照组采用阿昔洛韦膏外涂患处,5～6次／日;口服阿昔洛韦片0．2g／次,5次／日;肌肉注射聚肌胞注射液2mg,隔日1次。两组均以10日为1个疗程,1个疗程后判定疗效。结果：治疗组30例病人痊愈20例,好转8例,未愈2例。有效率93．33％。对照组30例,．痊愈11人,好转13人,未愈6人。有效率80．00％。结论：皮下针、点灸及刺络拔罐治疗带状疱疹见效快、疗效好,值得推广。     

银杏生发合剂促进小鼠毛发生长的作用研究

目的:观察银杏生发合剂对毛发生长的促进作用。方法:取C57BL/6小鼠,随机分为章光101组,银杏合剂高、中、低剂量组,将小鼠背部双侧脱发,左侧分别外擦章光101育发剂,银杏生发合剂167.7mg/ml、100.6mg/ml、33.5 mg/ml,右侧外涂30%乙醇溶剂,每日2次。观察21天内动物新生毛发生长情况,测定各组动物新生毛发长度及重量,采用ELISA法测定皮肤组织中血管内皮生长因子及Ⅰ型胶原含量。结果:银杏生发合剂高剂量能显著增加动物毛发生长速度(P<0.05),中剂量能显著增加动物新生毛发长度及皮肤中血管内皮生长因子含量(P<0.05)。结论:外擦银杏生发合剂有显著的促生发作用,并能增加皮肤中VEGF的分泌。     

昆布多糖硫酸酯对荷瘤小鼠前列腺癌的抑瘤作用

目的:研究昆布多糖硫酸酯(LAMS)对移植前列腺癌细胞RM-1荷瘤小鼠的抑瘤作用。方法:C57小鼠接种前列腺癌RM-1细胞7 d后,按瘤体大小将40只小鼠均分为4组,分别为模型组(NS),环磷酰胺(CY)组(20 mg.kg-1),LAMS低、高剂量组(50,100 mg.kg-1)i,g,1次/d,连续14 d,停药次日称体质量并处死动物,剥取瘤块,摘取脾脏和胸腺,称质量,计算抑瘤率、脾指数和胸腺指数。结果:CY 20 mg.kg-1抑瘤率为40.3%,LAMS低、高剂量组抑瘤率分别为25.0%和33.8%,与模型组比较均有显著差异;CY在抑瘤的同时能显著抑制荷瘤小鼠的胸腺指数和脾指数,与模型组比较有显著性差异,LAMS对荷瘤小鼠的胸腺指数和脾指数无明显影响。结论:LAMS对移植前列腺癌RM-1的荷瘤小鼠有一定的抑瘤作用,LAMS在抑制肿瘤的同时不影响荷瘤鼠的免疫功能,无CY等化疗药物的常见副作用。     

香菇多糖对小鼠T细胞比例及活化的影响

目的:研究香菇多糖对DTH小鼠T淋巴细胞比例及活化的影响。方法:用800mg/kg的香菇多糖给小鼠灌胃7天,给药后第3天用5%二硝基氟苯(DNFB)致敏,第4天用90mg/kg环磷酰胺造成免疫抑制,第7天用1%DNFB激发引起迟发型超敏反应(DTH),第8天取耳片及颈部淋巴结(CLN)细胞,称重法检测耳片肿胀度,流式细胞术检测CLN中CD3+、B220+细胞比例及T细胞中CD25+、CD69+表达。结果:香菇灌胃组耳片肿胀度和CLN中CD3+、B220+及CD3+CD25+、CD3+CD69+比例细胞均比模型组有所上升。结论:香菇多糖对免疫抑制小鼠的T细胞功能有改善作用。     

Acupuncture for poison ivy contact dermatitis. A clinical case report

Poison ivy contact dermatitis is fairly common in the suburbia of this country among amateur gardeners and children. It commonly inflicts its poison on the exposed parts of the limbs. The vesicular or bullous skin lesions are quite disturbingly itchy. Scratching the itchy lesions often spreads the condition by transplanting the remanent resinous toxin to other parts of the body. Though they are usually self-limiting, the intense itch is the main motivation for a patient to seek medical care. The conventional treatment is basically ineffective. During the summer of 1987 we treated four such cases of dermatitis with acupuncture upon their request to mollify their unbearable itch. They originally consulted with us for other problems. There were three males and one female. Their ages were between 29 and 63. Three cases were relatively mild and the fourth one was fairly severe. In the milder cases, their itch subsided in a few hours and skin lesions were healed in about two days after one treatment. In the severe case the itch subsided in about two days and most of the skin lesions dried up in four days after the first treatment and were healed almost completely after three sessions of acupuncture treatment. The plausible anti-inflammatory mechanism of acupuncture with the involvement of ACTH and/or cortisol was discussed.     

Immuno-enhancement effects of ethanol extract from Cyrtomium macrophyllum (Makino) Tagawa on cyclophosphamide-induced immunosuppression in BALB/c mice

Ethnopharmacological relevance

Cyrtomium macrophyllum (Makino) Tagawa has been traditionally used as a herbal medicine for the treatment of various infectious diseases such as tapeworm infestation, colds, and viral diseases. However, no systematic study of the immunity of Cyrtomium macrophyllum ethanol extracts (CM) has yet been reported. The present work evaluates these traits.

Materials and methods

120 male BALB/c mice were divided into 6 groups of 20 mice each: (1) normal group (sterile physiological saline), which served as a blank control; (2) model group (Cyclophosphamide, CY) group (sterile physiological saline), which served as a negative control; (3) low-dose CM (50 mg/kg BW); (4) intermediate-dose CM (100 mg/kg BW); (5) high-dose CM (200 mg/kg BW); (6) CM group (200 mg/kg BW). CY (0.2 ml) was administered via intraperitoneal injection. The other regimens were administered via gavage in 0.2 ml solution. Phytochemical of CM was characterized by HPLC–LTQ-Orbitrap. The acute toxicity effect of the ethanol extract of Cyrtomium macrophyllum was also investigated.

Results

The spleen and thymus indices of mice receiving low, intermediate, and high doses of CM recovered more quickly than those of CY mice, and they did so in a dose-dependent manner. These mice also showed higher T cell and B cell proliferation responses and macrophage function than those of CY mice, and their serum levels of interleukin-6 and interferon-γ had become normal. In acute toxicity test, CM exhibited no mortality and behavioral changes in mice. Quantitative phytochemical analysis showed flavonoids, polyphenols, and tannins to be the major compounds present in the extract, at 27.64%, 30.87%, and 11.22%, respectively. We found that 16 compounds were characterized by the interpretation of their mass spectra obtained by the MS/MS.

Conclusion

The current study demonstrates that Cyrtomium macrophyllum ethanol extract improved immune function in CY-treated mice.     

Hepatoprotective activity of Thunbergia laurifolia Linn extract in rats treated with ethanol: in vitro and in vivo studies

Primary cultures of rat hepatocyte and rats were used as the in vitro and in vivo models to evaluate the hepatoprotective activity of aqueous extract from Thunbergia laurifolia (TLE). Ethanol was selected as hepatotoxin. Silymarin (SL) was the reference hepatoprotective agent. In the in vitro study, MTT reduction assay and release of transaminases (ALT and AST) were the criteria for cell viability. Primary cultures of rat hepatocyte (24 h culturing) were treated with ethanol (96 microl/ml) and various concentrations of TLE (2.5, 5.0, 7.5 and 10.0 mg/ml) or SL (1, 2 and 3 mg/ml) for 2 h. Ethanol decreased MTT (%) nearly by half. Both TLE and SL increased MTT reduction and brought MTT (%) back to normal. Ethanol induced release of ALT and AST was also reduced by TLE (2.5 and 5.0 mg/ml) and SL (1 mg/ml). In the in vivo study, serum transaminases, serum triglyceride (STg) together with hepatic triglyceride (HTg) and histopathological examination were the criteria for evidences of liver injury. Ethanol (4 g/(kg day), po for 14 days) caused the increase in ALT, AST, HTg and centrilobular hydropic degeneration of hepatocytes. TLE at 25 mg/(kg day), po, or SL at 5 mg/(kg day), po, for 7 days after ethanol enhanced liver cell recovery by bringing HTg, ALT and/or AST back to normal. These results suggest that TLE and SL possess the hepatoprotective activity against ethanol induced liver injury in both primary cultures of rat hepatocyte and rats.