昆布多糖硫酸酯对荷瘤小鼠前列腺癌的抑瘤作用

目的:研究昆布多糖硫酸酯(LAMS)对移植前列腺癌细胞RM-1荷瘤小鼠的抑瘤作用。方法:C57小鼠接种前列腺癌RM-1细胞7 d后,按瘤体大小将40只小鼠均分为4组,分别为模型组(NS),环磷酰胺(CY)组(20 mg.kg-1),LAMS低、高剂量组(50,100 mg.kg-1)i,g,1次/d,连续14 d,停药次日称体质量并处死动物,剥取瘤块,摘取脾脏和胸腺,称质量,计算抑瘤率、脾指数和胸腺指数。结果:CY 20 mg.kg-1抑瘤率为40.3%,LAMS低、高剂量组抑瘤率分别为25.0%和33.8%,与模型组比较均有显著差异;CY在抑瘤的同时能显著抑制荷瘤小鼠的胸腺指数和脾指数,与模型组比较有显著性差异,LAMS对荷瘤小鼠的胸腺指数和脾指数无明显影响。结论:LAMS对移植前列腺癌RM-1的荷瘤小鼠有一定的抑瘤作用,LAMS在抑制肿瘤的同时不影响荷瘤鼠的免疫功能,无CY等化疗药物的常见副作用。

Inhibitory Effect of LAMS Against Prostatic Cancer in Tumor-earing Mice

Objective: To study the inhibitory effect of LAMS on prostatic cancer growth in tumor-bearing mice. Method: Fourty C57 mice inoculated with RM-1 prostate cancer cells for 7 days were divided into 4 groups according to the tumor size:the model group (NS),cyclophosphamide (CY) group (20 mg ·kg^-1),LAMS low and high dose groups (50,100 mg ·kg^-1),ig,once daily. After 14 days of treatment,the animals were killed. The subcutaneous sarcoma,thymus and spleen were separated and weighted. Inhibition rate,spleen index and thymus index were then calculated. Result: CY 20 mg ·kg^-1 significantly inhibited the growth of tumor,the inhibition rate was 40.3%;LAMS(low and high dose) also showed significant inhibitory effect compared with the model group,the inhibition rates were 25.0% and 33.8%. CY could significantly inhibited the thymus index and spleen index in tumor-bearing mice,however,the effects of LAMS were not significant. Conclusion: LAMS have inhibitory effects on RM-1 in tumor-bearing mice,but do not affect the immune function. Furthermore,LAMS has no side effects on chemotherapy drugs such as CY.