皮肤老化分子机制及中药防治皮肤老化研究进展

衰老又称老化,皮肤作为人体最大的器官,其老化是人体衰老最直接具体的表现。皮肤衰老是内源性因素和外源性因素共同作用的结果,主要包括自然老化和光老化。两者在临床和组织学上存在明显差异,但具有相似的分子机制。中药具有安全可靠、不良反应小等特点,在防治皮肤老化方面有着积极的作用。本文重点综述了皮肤光老化的分子机制,总结了光老化与自然老化分子机制方面的相似点,并介绍了中药防治皮肤老化的研究进展,为中药预防治疗及延缓皮肤老化乃至整体衰老提供理论支持。     

虫草多糖对8-MOP／UVA诱导光老化皮肤成纤维细胞胶原的影响

目的：探讨虫草多糖（CP）对皮肤成纤维细胞光老化保护作用中对胶原的影响。方法：用8-甲氧补骨脂素联合UVA（8-MOP／UVA）诱导皮肤成纤维细胞光老化,用电镜观察、羟脯氨酸含量、丙二醛含量测定及免疫细胞化学等方法观察虫草多糖对成纤维细胞光老化过程中胶原的影响。结果：光老化组细胞较对照组蛋白合成功能不活跃,羟脯氨酸含量下降,丙二醛含量升高,Ⅰ型胶原表达降低,Ⅲ型胶原表达增高,虫草保护组细胞有所改善。结论：虫草多糖对8-MOP／UVA诱导光老化的皮肤成纤维细胞胶原变化具有逆转作用。     

中草药防治皮肤光老化机制研究现状

中草药在防治皮肤光老化方面有着积极的作用,文章对中草药防治皮肤光老化的研究现状进行综述,总结其防治皮肤光老化的机制主要有:清除自由基,抗氧化,调解免疫功能,刺激成纤维细胞的活性,促进胶原蛋白的合成及修复。并提出中草药防治皮肤光老化的研究应结合现代医学研究观点及传统中医理论从多角度、多层次进行。     

补肾化瘀法抗光老化对基质金属蛋白酶MMP-1及MMP-3表达的影响

【目的】探讨补肾化瘀法抗光老化对基质金属蛋白酶MMP-1与MMP-3表达的影响。【方法】采用8-甲氧沙林(8-MOP)/长波紫外线照射SD大鼠法,复制光老化大鼠模型,并对该动物模型给予补肾化瘀中药干预,连续15 d,采用免疫组织化学SP法,分别检测空白对照组、补肾化瘀中药干预组和光老化模型组大鼠皮肤MMP-1、MMP-3的表达,使用图像分析软件分析各组照片的单位灰度值并进行比较,根据灰度值的大小判断MMP-1、MMP-3的表达水平。【结果】建立了光老化大鼠模型。经过免疫组织化学染色显示,补肾化瘀中药干预组大鼠皮肤切片中阳性染色颗粒明显减少,MMP-1和MMP-3表达显著减弱。使用图像分析软件分析结果显示,光老化模型组大鼠皮肤中MMP-1和MMP-3表达显著高于空白对照组,差异有统计学意义(P0.05);中药干预组大鼠皮肤中MMP-1和MMP-3表达水平显著降低,与模型对照组比较,差异有统计学意义(P0.05)。【结论】补肾化瘀中药能显著降低光老化模型大鼠皮肤中MMP-1和MMP-3的表达水平,促进光老化大鼠皮肤损害的修复,具有一定的抗光老化作用。     

维甲酸霜对皮肤光老化的防治作用

老化是由许多内在及外在的因素促成的一种复杂过程。由于皮肤是暴露在外的器官，所以皮肤老化除自然老化（内源性因素）外，还有光老化（外源性因素），有的学者认为暴露部位皮肤的老化，９０％是紫外线光老化作用的结果。因此，防治光老化具有实际的意义。近年来维甲酸霜...     

皮肤光老化中医机理探讨

人体皮肤每天都接受来自日光中紫外线的辐射,长期辐射会引起人体暴露部位皮肤光老化。阐明其机理对防护皮肤光损伤及皮肤衰老甚至皮肤癌具有十分重要的意义。现代医学对皮肤光老化发病机理已经做了深入研究。目前,光老化尚无明确的中医理论记载,文章主要对皮肤光老化的中医病因病机作以探讨。     

甲基莲心碱对紫外线致人皮肤成纤维细胞形态结构的影响

目的:探讨甲基莲心碱对紫外线致人成纤维细胞形态结构的影响,为防治皮肤光老化性疾病提供实验依据。方法:建立中长波紫外线辐射人皮肤成纤维细胞光老化模型,实验分为对照组、模型组(单纯紫外线照射)和药物组(紫外线照射加甲基莲心碱干预),观察和分析成纤维细胞经紫外线照射后细胞生长形态和超微结构变化。结果:紫外线照射成纤维细胞后,细胞失去正常形态,超微结构显示线粒体损伤,而经甲基莲心碱处理后细胞损伤明显减轻。结论:适当浓度(0.8μM)的甲基莲心碱能减轻紫外线照射后成纤维细胞的结构损伤,为防治皮肤光老化疾病提供了新的治疗思路。     

皮肤光老化的控制方法

皮肤长期暴露于紫外线下会发生形态学、组织学和生理学的改变,称之为光老化。皮肤的光老化是造成皮肤老化的主要原因,目前大多数人认为皮肤光老化加速了皮肤自然老化的进程[1]。在临床上,光老化可表现为暴露部位皮肤松弛、粗深皱纹、结节、皮革样外观、色素斑增多、毛细血管扩张、肤色常呈灰黄。其始于儿童时期,20岁后人的容颜就开始出现老化征兆,比自然老化年龄要平均提前10年[2]。近年来,皮肤光老化的问题业已受到国内外人们越来越多的关注,认为皮肤光老化许多改变,如粗细皱纹、皮肤粗糙及色素沉着的药物改善和逆转是可能的,从而在对其预…     

光老化及其机制

人体的皮肤每天都接受来自日光中紫外线的辐射,其长期辐射可导致皮肤光老化。近年来,随着空气污染所致的臭氧层变薄,紫外线更易通过大气层,从而加重暴露皮肤的光损害。皮肤光老化可与多种皮肤病甚至与皮肤肿瘤的发生有密切关系,本文主要描述了紫外线造成的皮肤光老化及其机制。     

皮肤光老化的控制方法

皮肤长期暴露于紫外线下会发生形态学、组织学和生理学的改变，称之为光老化。皮肤的光老化是造成皮肤老化的主要原因，目前大多数人认为皮肤光老化加速了皮肤自然老化的进程。在临床上，光老化可表现为暴露部位皮肤松弛、粗深皱纹、结节、皮革样外观、色素魔：噌多、毛细血管扩张、肤色常呈灰黄。其始于儿童时期，20岁后人的容颜就开始出现老化征兆，比自然老化年龄要平均提前10年。近年来，皮肤光老化的问题业已受到国内外人们越来越多的关注，认为皮肤光老化许多改变，如粗细皱纹、皮肤粗糙及色素沉着的药物改善和逆转是可能的，从而在对其预防和治疗方面也作为一个独立的疾病或综合症进行研究。同时，     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.