海水和淡水肺内灌注对兔肺损伤作用的实验观察

Objective To investigate differences in lung injury induced by seawater and freshwater perfusion in rabbits. Methods 21 New Zealand rabbits were randomly divided into the normal group (the control group), the freshwater group and the seawater group. Same amounts of seawater and freshwater were perfused in the lungs of rabbits through tracheal incubation. Changes in the indices concerning symptomatology, blood-gas, hemodynamics, tumor necrosis factor (TNF-α), interleukin 6 (IL-6), histology and lung injury were monitored 3 hours later. Then, the obtained data were analyzed statistically to see the differences in lung lesions of different animal groups. Results Blood-gas analysis, inflammatory factor measurement, lung injury score indicated that seawater and freshwater perfusion could all induce lung injury to a certain extent, and the injury induced by seawater perfusion was obviously severer(P<0.05). Conclusions Lung injury induced by seawater perfusion is severer than that by freshwater perfusion.     

海水和淡水肺内灌注对兔肺损伤作用的实验观察

Objective To investigate differences in lung injury induced by seawater and freshwater perfusion in rabbits. Methods 21 New Zealand rabbits were randomly divided into the normal group (the control group), the freshwater group and the seawater group. Same amounts of seawater and freshwater were perfused in the lungs of rabbits through tracheal incubation. Changes in the indices concerning symptomatology, blood-gas, hemodynamics, tumor necrosis factor (TNF-α), interleukin 6 (IL-6), histology and lung injury were monitored 3 hours later. Then, the obtained data were analyzed statistically to see the differences in lung lesions of different animal groups. Results Blood-gas analysis, inflammatory factor measurement, lung injury score indicated that seawater and freshwater perfusion could all induce lung injury to a certain extent, and the injury induced by seawater perfusion was obviously severer(P<0.05). Conclusions Lung injury induced by seawater perfusion is severer than that by freshwater perfusion.     

海水和淡水肺内灌注对兔肺损伤作用的实验观察

Objective To investigate differences in lung injury induced by seawater and freshwater perfusion in rabbits. Methods 21 New Zealand rabbits were randomly divided into the normal group (the control group), the freshwater group and the seawater group. Same amounts of seawater and freshwater were perfused in the lungs of rabbits through tracheal incubation. Changes in the indices concerning symptomatology, blood-gas, hemodynamics, tumor necrosis factor (TNF-α), interleukin 6 (IL-6), histology and lung injury were monitored 3 hours later. Then, the obtained data were analyzed statistically to see the differences in lung lesions of different animal groups. Results Blood-gas analysis, inflammatory factor measurement, lung injury score indicated that seawater and freshwater perfusion could all induce lung injury to a certain extent, and the injury induced by seawater perfusion was obviously severer(P<0.05). Conclusions Lung injury induced by seawater perfusion is severer than that by freshwater perfusion.     

海水和淡水肺内灌注对兔肺损伤作用的实验观察

Objective To investigate differences in lung injury induced by seawater and freshwater perfusion in rabbits. Methods 21 New Zealand rabbits were randomly divided into the normal group (the control group), the freshwater group and the seawater group. Same amounts of seawater and freshwater were perfused in the lungs of rabbits through tracheal incubation. Changes in the indices concerning symptomatology, blood-gas, hemodynamics, tumor necrosis factor (TNF-α), interleukin 6 (IL-6), histology and lung injury were monitored 3 hours later. Then, the obtained data were analyzed statistically to see the differences in lung lesions of different animal groups. Results Blood-gas analysis, inflammatory factor measurement, lung injury score indicated that seawater and freshwater perfusion could all induce lung injury to a certain extent, and the injury induced by seawater perfusion was obviously severer(P<0.05). Conclusions Lung injury induced by seawater perfusion is severer than that by freshwater perfusion.     

海水和淡水肺内灌注对兔肺损伤作用的实验观察

Objective To investigate differences in lung injury induced by seawater and freshwater perfusion in rabbits. Methods 21 New Zealand rabbits were randomly divided into the normal group (the control group), the freshwater group and the seawater group. Same amounts of seawater and freshwater were perfused in the lungs of rabbits through tracheal incubation. Changes in the indices concerning symptomatology, blood-gas, hemodynamics, tumor necrosis factor (TNF-α), interleukin 6 (IL-6), histology and lung injury were monitored 3 hours later. Then, the obtained data were analyzed statistically to see the differences in lung lesions of different animal groups. Results Blood-gas analysis, inflammatory factor measurement, lung injury score indicated that seawater and freshwater perfusion could all induce lung injury to a certain extent, and the injury induced by seawater perfusion was obviously severer(P<0.05). Conclusions Lung injury induced by seawater perfusion is severer than that by freshwater perfusion.     

海水和淡水肺内灌注对兔肺损伤作用的实验观察

Objective To investigate differences in lung injury induced by seawater and freshwater perfusion in rabbits. Methods 21 New Zealand rabbits were randomly divided into the normal group (the control group), the freshwater group and the seawater group. Same amounts of seawater and freshwater were perfused in the lungs of rabbits through tracheal incubation. Changes in the indices concerning symptomatology, blood-gas, hemodynamics, tumor necrosis factor (TNF-α), interleukin 6 (IL-6), histology and lung injury were monitored 3 hours later. Then, the obtained data were analyzed statistically to see the differences in lung lesions of different animal groups. Results Blood-gas analysis, inflammatory factor measurement, lung injury score indicated that seawater and freshwater perfusion could all induce lung injury to a certain extent, and the injury induced by seawater perfusion was obviously severer(P<0.05). Conclusions Lung injury induced by seawater perfusion is severer than that by freshwater perfusion.     

海水和淡水肺内灌注对兔肺损伤作用的实验观察

Objective To investigate differences in lung injury induced by seawater and freshwater perfusion in rabbits. Methods 21 New Zealand rabbits were randomly divided into the normal group (the control group), the freshwater group and the seawater group. Same amounts of seawater and freshwater were perfused in the lungs of rabbits through tracheal incubation. Changes in the indices concerning symptomatology, blood-gas, hemodynamics, tumor necrosis factor (TNF-α), interleukin 6 (IL-6), histology and lung injury were monitored 3 hours later. Then, the obtained data were analyzed statistically to see the differences in lung lesions of different animal groups. Results Blood-gas analysis, inflammatory factor measurement, lung injury score indicated that seawater and freshwater perfusion could all induce lung injury to a certain extent, and the injury induced by seawater perfusion was obviously severer(P<0.05). Conclusions Lung injury induced by seawater perfusion is severer than that by freshwater perfusion.     

海水和淡水肺内灌注对兔肺损伤作用的实验观察

Objective To investigate differences in lung injury induced by seawater and freshwater perfusion in rabbits. Methods 21 New Zealand rabbits were randomly divided into the normal group (the control group), the freshwater group and the seawater group. Same amounts of seawater and freshwater were perfused in the lungs of rabbits through tracheal incubation. Changes in the indices concerning symptomatology, blood-gas, hemodynamics, tumor necrosis factor (TNF-α), interleukin 6 (IL-6), histology and lung injury were monitored 3 hours later. Then, the obtained data were analyzed statistically to see the differences in lung lesions of different animal groups. Results Blood-gas analysis, inflammatory factor measurement, lung injury score indicated that seawater and freshwater perfusion could all induce lung injury to a certain extent, and the injury induced by seawater perfusion was obviously severer(P<0.05). Conclusions Lung injury induced by seawater perfusion is severer than that by freshwater perfusion.     

胸部开放伤后海水浸泡对犬肺损伤程度的影响及肺表面活性蛋白A、B的变化

Objective To compare the extent of acute lung injury (ALl) and changes in surfactant proteins A (SP-A) and B (SP-B) induced by seawater and freshwater immersion following open chest injury in dogs. Methods The animal model was established by seawater and freshwater injection into the thoracic cavity after open chest injury. All the experimental animals were observed for 6 hours, during which blood gas analysis, levels of TNF-α and IL-8, contents of SP-A and SP-B in the lung and blood serum were measured at different time points. Pulmonary histopathology and SP-B immuno-histochemistry were measured after the animals were sacrificed at the end of the experiment. Results Both seawater immersion and freshwater immersion could induce hypoxemia, with the extent of hypoxemia for the seawater immersion group (SG) being obviously much severer than that of the freshwater immersion group (FG) (P < 0.05). The levels of IL-8 and TNF-α in blood serum and BALF of both experimental groups were significantly higher than those of the control group (CG) (P<0.05). Pulmonary pathological lesion was noted in both SG and FG, with that of SG being severer than that of FG. The levels of SP-A and SP-B in BALF in SG and FG all decreased significantly(P <0.05) ,while the levels of SP-A and SP-B in blood serum increased gradually (P < 0.05). Type Ⅱ alveolarepithelial cells with SP-B positive in the lung decreased in both experimental groups, with grey scale values being significantly lower than that of CG. And significant deviation was observed between SG and FG (P <0.05). Conclusions Pulmonary lesion induced by seawater immersion after open chest injury was severer than that induced by freshwater immersion.     

胸部开放伤后海水浸泡对犬肺损伤程度的影响及肺表面活性蛋白A、B的变化

Objective To compare the extent of acute lung injury (ALl) and changes in surfactant proteins A (SP-A) and B (SP-B) induced by seawater and freshwater immersion following open chest injury in dogs. Methods The animal model was established by seawater and freshwater injection into the thoracic cavity after open chest injury. All the experimental animals were observed for 6 hours, during which blood gas analysis, levels of TNF-α and IL-8, contents of SP-A and SP-B in the lung and blood serum were measured at different time points. Pulmonary histopathology and SP-B immuno-histochemistry were measured after the animals were sacrificed at the end of the experiment. Results Both seawater immersion and freshwater immersion could induce hypoxemia, with the extent of hypoxemia for the seawater immersion group (SG) being obviously much severer than that of the freshwater immersion group (FG) (P < 0.05). The levels of IL-8 and TNF-α in blood serum and BALF of both experimental groups were significantly higher than those of the control group (CG) (P<0.05). Pulmonary pathological lesion was noted in both SG and FG, with that of SG being severer than that of FG. The levels of SP-A and SP-B in BALF in SG and FG all decreased significantly(P <0.05) ,while the levels of SP-A and SP-B in blood serum increased gradually (P < 0.05). Type Ⅱ alveolarepithelial cells with SP-B positive in the lung decreased in both experimental groups, with grey scale values being significantly lower than that of CG. And significant deviation was observed between SG and FG (P <0.05). Conclusions Pulmonary lesion induced by seawater immersion after open chest injury was severer than that induced by freshwater immersion.