纤维连结蛋白,粘附抗原和造血祖细胞

纤维连结蛋白（ＦＮ）是广泛存在于多种细胞表面、基质及血浆中的一种糖蛋白，在细胞粘附、迁移、分化及成熟中的作用已有较多研究。但ＦＮ与髓系祖细胞分化成熟的关系研究尚少。除ＢＦＵ－Ｅ、ＣＦＵ－Ｅ和ＣＦＵ－ＧＭ皆能和ＦＮ分子中ＲＧＤ短肽相结合外，最近发现，原始造血祖细胞（ＬＴＢＭＣ－ＩＣ）多向造血祖细胞（ＣＦＵ－ＭＩＸ）、ＢＦＵ－Ｅ、ＣＦＵ－Ｅ及ＣＦＵ－ＧＭ还能和ＦＮ分子肝素结合片段中的“ＣＳ１”多肽结合     

血性脑脊液对内皮功能影响及药物干预的研究

目的 观察血性脑脊液（ＢＣＳＦ）对内皮细胞（ＥＣ）分泌内皮素（ＥＴ）和一氧化氮（ＮＯ）的影响及川芎嗪（ＬＺ）、尼莫地平（ＮＭ）的干预作用。方法建立脐静脉ＥＣ体外培养的方法,测定ＥＴ、ＮＯ的代谢产物ＮＯ_Ｘ－ 含量,并观察ＢＣＳＦ和ＮＭ及ＬＺ的作用。结果ＬＺ和ＮＭ可抑制正常体外培养的ＥＣ分泌ＥＴ,对ＮＯ无影响;不同时间ＢＣＳＦ均使ＥＴ分泌增加,ＮＯ分泌减少;ＬＺ既能减少ＢＣＳＦ引起的ＥＴ升高,又可抑制ＮＯ的下降,而ＮＭ仅能抑制ＥＴ的增高,对ＮＯ无明显影响。结论蛛网膜下腔出血（ＳＡＨ）后,通过破坏ＥＴ／ＮＯ的平衡,导致脑血管痉挛（ＣＶＳ）。ＬＺ和ＮＭ可通过抑制这一作用而防治ＣＶＳ。     

成人慢性髓性白血病细胞β1整合素功能障碍的研究

为了探讨Ｐｈ（＋）慢性髓性白血病（ＣＭＬ）骨髓细胞是否存在β１整合素活化障碍,我们以１０例正常及１２例ＣＭＬ骨髓单个核细胞（ＮＢＭＭＮＣ及ＣＭＬＢＭＭＮＣ）为研究对象,用β１整合素活化抗体８Ａ２和粒巨噬系集落刺激因子（ＧＭ－ＣＳＦ）作活化剂,用流式细胞术测定上述细胞与可溶性纤连蛋白（ＦＮ）的结合能力及其β１整合素跨膜区９ＥＧ７表位的表达,并观察了ＡＢＬ激酶抑制剂ＣＧＰ５７１４８Ｂ预处理对上述细胞β     

caspase 3在细胞因子调节急性白血病细胞凋亡中的变化

目的 研究ｃａｓｐａｓｅ３在粒细胞集落刺激因子（Ｃ－ＣＳＦ）和粒巨洚细胞集落因子（ＧＭ－ＣＳＦ）调节白血病细胞系ＮＢ４细胞凋亡过程中的变化及其意义。方法 利用细胞这、流式细胞仪分析及ＤＮＡ片段化比率测定,检测Ｇ－ＣＳＦ及ＤＭ－ＣＳＦ对ＮＢ４细胞凋亡的影响,应用荧光分光光度法检测ｃａｓｐａｓｅ３活性变化,并利用ＡＥ－ＤＥＶＤ－ＣＲＯ进行ｃａｓｐａｓｅ３抑制试验。结果 Ｇ－ＣＳＦ能诱导ＮＢ４细胞凋亡,     

三丁酸甘油酯对早幼粒细胞白血病细胞株的诱导分化及促凋亡作 …

目的 研究三丁酸甘油酯（ＴＢ）与全反式维甲酸（ＡＴＲＡ）不同浓度组合对ＮＢ４及ＭＲ２细胞的诱导分化作用,并观察ＴＢ对ＮＢ４,ＭＲ２细胞是否具有促凋亡作用。方法 通过硝基四唑氮蓝（ＮＢＴ）还原率及细胞表面抗原ＣＤ１１ｂ、ＣＤ１４、ＣＤ３３表达观察细胞分化程度;通过细胞形态、ＤＮＡ片段化电泳,流式细胞术（ＦＣＭ）ＤＮＡ含量分析及凋亡细胞原位标记（ＴＵＮＥＬ）试剂盒检测细胞凋亡;用逆转录－聚合酶链反应（     

逆转录-聚合酶链反应检测人外周血单个核细胞中γ干扰素的表达

探讨检测γ－干扰素（ＩＦＮγ）自然表达状况的方法。用多重逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）法检测了１５例ＳＬＥ患者和１０名正常人外周血单个核细胞（ＰＢＭＣ）中ＩＦＮγmＲＮＡ表达的水平。以异硫氰酸胍－酚－氯仿。一步法提取总ＲＮＡ，逆转录后同管扩增β－actin与ＩＦＮγ，扩增产物电泳后经光密度扫描仪扫描。扩增后β－actin与ＩＦＮγ条带清晰可见。与正常人相比，活动期ＳＬＥ患者ＰＢＭＣ中ＩＦＮγmＲＮＡ表     

急性与慢性再生障碍性贫血患者IL—4,IFN—γ及TNF—α诱生水 …

目的 检测急性和慢性再生障碍性贫血（再障）患者骨髓单个核细胞（ＢＭＭＮＣ）培养上清白细胞介素４（ＩＬ－４）、γ干扰素（ＩＦＮ－γ）及肿瘤坏死因子α（ＴＮＦ－α）的诱生水平。了解两组患者免疫学状况的异同点,方法 利用双抗体夹心ＥＬＩＳＡ法测定ＢＭＭＮＣ培养上清中ＩＬ－４、ＩＦＮ－γ和ＴＮＦ－α的诱生水平,比较两组患者上述细胞因子水平的差异。结果 （１）所有患者ＩＦＮ－γ、ＴＮＦ－α水平均增高,与对照     

流式细胞术在程序性细胞死亡研究中的应用

本文对流式细胞（ＦＣＭ）在程序性细胞死亡研究中的作用作了综述，包括ＰＩ染色法、Ｈｏｅｃｈｓｔ－ＰＩ双染色法、ＩＳＮＴ或ＴＵＮＥＬ技术及ＦＣＭ在程序性细胞死亡相关基因表达和信号转导机制研究中应用。     

超声心动图及多普勒超声探测非胰岛素依赖型糖尿病患者左室功能

应用超声心图及多普勒超声检测１０３例非胰岛素依赖型糖尿病（ＮＩＤＤＭ）患者和５１例健康人左心室功能和结构参数。结果：各组ＮＩＤＤＭ患者左室舒张功能均有异常，收缩功能障碍仅见于伴大血管病变者。与正常人比较，糖尿病患者ＰＶＡ和ＰＶＡ／ＰＶＥ升高，ＰＶＥ和ＭＥＦ降低；ＬＶＳＴ、ＬＶＰＷＴ、ＬＶＭ和ＬＶＭＩ增加；伴大血管变者ＳＶ、ＣＯ及ＥＦ均减少。结论：ＮＩＤＤＭ患者常见左室功能障碍，甚至可见于无血管并发     

流式细胞术在程序性细胞死亡研究中的应用

本文对流式细胞术（ＦＣＭ）在程序性细胞死亡研究中的作用作了综述，包括ＰＩ染色法、Ｈｏｅｃｈｓｔ－ＰＩ双染色法、ＩＳＮＴ或 ＴＵＮＥＬ技术及 ＦＣＭ在程序性细胞死亡相关基因表达和信号转导机制研究中应用。     

经腹和经阴道超声联合应用鉴别诊断子宫角部和输卵管间质部妊娠

目的探讨经腹和经阴道超声检查在子宫角部妊娠和输卵管间质部妊娠鉴别诊断中的价值。方法列经手术病理证实的18例子宫角部妊娠和23例输卵管间质部妊娠的声像图特点进行对比分析。结果41例子宫角部妊娠和输卵管间质部妊娠超声表现为孕囊型和混合型两种类型。孕囊型共30例,孕囊位于子宫角部14例,超声诊断符合率92.8%(13/14);超声表现为孕囊位于膨大的宫角内,与子宫内膜相连或隐约相连;孕囊周围有厚度均匀的肌层组织.厚9～12 mm。孕囊位于输卵管间质部16例,超声诊断符合率93.8%(15/16)。超声表现为宫角有不同程度的膨出.孕囊边缘与子宫内膜存在2～7 mm,不等的距离;孕囊周围肌层组织厚度<9mm。混合型共11例,宫角妊娠4例,间质部妊娠7例。结论联合应用经腹和经阴道超声检查有助于对子宫角部妊娠和输卵管间质部妊娠的孕囊型鉴别,有一定的临床意义。     

瘢痕子宫再次妊娠待产妇心理健康状况评价及其影响因素

目的探究瘢痕子宫再次妊娠待产妇心理健康状况及其影响因素。方法选择医院2016年11月—2019年11月收治的瘢痕子宫再次妊娠待产妇90例,根据待产妇心理健康状况评分,分为心理健康状况较差组20例,良好组70例。借助一般资料量表调查基础信息,选择症状自评量表(SCL-90)调查其心理健康状况,通过多因素Logistic回归分析筛选瘢痕子宫再次妊娠待产妇心理健康状况的影响因素。结果调查结果显示,瘢痕子宫再次妊娠待产妇心理健康状况评分为145.48±12.12分,总体心理健康状况良好。单因素分析显示,年龄、家庭经济水平、孕期身体状况、孕期并发症、妊娠反应、睡眠质量、社会支持、家庭关怀度、负性生活事件、不良妊娠经历、周围朋友瘢痕子宫再次妊娠不良妊娠结局对瘢痕子宫再次妊娠待产妇心理健康状况影响比较差异具有统计学意义(P<0.05);经多因素Logistic回归分析发现,瘢痕子宫再次妊娠待产妇心理健康状况的影响因素有孕期并发症、不良妊娠经历、负性生活事件、周围朋友瘢痕子宫再次妊娠不良妊娠结局、睡眠质量差、社会支持不足、家庭关怀度低(P<0.05)。结论瘢痕子宫再次妊娠待产妇总体心理状况良好,但仍有部分患者心理健康状况较差,且其影响因素复杂,临床应据此确定护理方案,强化护理干预。     

131例妊娠期肝内胆汁淤积症患者的护理

目的 降低妊娠期肝内胆汁淤积症(ICP)患者母儿并发症的发生。方法 对13l例ICP孕产妇进行孕期、产时及产后严密的观察和护理，有针对性地进行ICP有关知识宣教；动态监测孕妇血中胆汁酸、转氨酶及胆红素水平；加强胎儿监护，及早发现隐性胎儿窘迫，适时终止妇娠。结果 13l例ICP患者母儿健康和安全都不同程度地受到了危害，但通过严密的观察和精心的护理，母儿并发症的发生较文献报道有较大程度的降低。结论 必须重视孕产期保健，早发现、早治疗ICP，严密观察和精心护理对降低患者母儿并发症有积极作用.     

子宫角部位妊娠的超声诊断及其误诊分析

目的总结超声在诊断宫角部位妊娠过程中的经验教训。方法回顾性分析12例宫角部位妊娠病例的临床与声像图特点。结果12例中仅1例由超声直接明确诊断,2例经临床观察及反复超声检查最终由阴道彩色多普勒超声诊断,其余均在术后确诊。结论超声可以为临床早期诊断宫角部妊娠提供影像学依据,经阴道彩色多普勒超声可作为该类疾病诊断的首选方法。     

Perforin-expressing granulated metrial gland cells in murine deciduoma

It has previously been shown that granulated metrial gland (GMG) cells of the pregnant uterus express abundant quantities of the lymphocyte pore-forming protein, perforin. No perforin was present before implantation of the embryo, but large numbers of perforin-producing GMG cells were observed after implantation, which coincides with decidualization of the uterus. The possible source of the activation factors responsible for perforin gene induction in GMG cells was studied here with the pseudopregnancy model, in which cervical stimulation of mice during estrus leads to a series of hormonal changes resembling those seen in pregnancy, but in the absence of an embryo. Subsequent stimulation of the uterus of pseudopregnant mice with oil causes the stimulated portion of the endometrium to differentiate into decidual tissue. Perforin-containing GMG cells were in fact present in the deciduomata, but not in adjacent nondecidualized tissues of the same mice. These results suggest that maternal factors associated with decidual tissue are responsible for the local expression of perforin in GMG cells.     

Preeclampsia is associated with abnormal expression of adhesion molecules by invasive cytotrophoblasts.

In normal human pregnancy, invasion of the uterus and its arterial system by cytotrophoblasts extends through the entire decidua and the adjacent third of the myometrium. Our previous work showed that during the first trimester of pregnancy, invasion is accompanied by a marked change in the expression of cell adhesion molecules by invasive cytotrophoblasts. In the pregnancy disorder preeclampsia, cytotrophoblast invasion is limited to the superficial decidua, and few arterioles are breached. The purpose of this study was to determine whether cytotrophoblast expression of adhesion molecules in this disorder is also abnormal. Placental bed biopsy specimens from normal pregnancies and those complicated by preeclampsia were stained with anti-integrin antibodies. The results showed that adhesion molecule switching by invasive cytotrophoblasts is abnormal in preeclampsia, which suggests that this subpopulation of trophoblast cells fails to differentiate properly. A likely result is that the delicate balance of adhesive interactions that normally permit cytotrophoblast invasion is tipped in favor of those which restrain this process, with the net effect of shallow uterine invasion.     

肝硬化失代偿合并妊娠瘢痕子宫患者中期引产一例的护理

目的探讨肝硬化失代偿期合并妊娠瘢痕子宫患者中期引产的护理方法。方法回顾性分析2010年北京佑安医院收治的1例肝硬化失代偿期合并妊娠瘢痕子宫患者的临床资料。患者入院后,即对其进行心理护理,制定有针对性的预防并发症发生的护理措施,指导患者饮食。结果经过积极有效的治疗和护理,该患者未发生严重并发症,引产过程顺利,预后良好,病情平稳后转入内科继续治疗。结论肝炎肝硬化失代偿合并妊娠瘢痕子宫的患者在实施中期引产时,风险大,并发症多。密切观察患者病情变化,采取有效的治疗措施,制定以"病人为中心"的个性化护理,是预防并发症发生和救治成功的关键。     

Intussusception: a rare cause of abdominal pain in pregnancy.

A 34-year-old woman presented at 19 weeks in her third pregnancy with abdominal pain and hyperemesis. This was her third admission during the pregnancy for similar complaints. A few days after admission an exacerbation in her pain was noted, in particular on eating or lying down, and a firm and mobile epigastric mass could be palpated separate from her uterus. The differential diagnosis was a hernia or a degenerating pedunculated fibroid. Sonography revealed a mass separate from the uterus with an appearance consistent with intussusception. Magnetic resonance imaging confirmed the diagnosis. A limited right hemicolectomy was performed. The final diagnosis was adenocarcinoma of the colon. It is difficult to diagnose intussusception during pregnancy. The presenting symptoms of nausea, vomiting, abdominal pain and constipation are common in pregnancy and the displacement of the bowel by the gravid uterus hampers examination. Intussusception is very rare in adults and generally it is associated with tumors. Preoperative diagnosis is difficult but possible with accurate imaging.     

In vitro tolerance induction of neonatal murine B cells as a probe for the study of B-cell diversification

The susceptibility to in vitro tolerance induction has been implicated as a characteristic of B cells early in their development, since DNP-reactive B cells are tolerizable only during the first days after birth, and 25% of adult bone marrow cells are tolerizable. In the present study, a modification of the in vitro splenic focus technique was utilized to determine if PC-specific B cells, by virtue of their late expression (approximately 1 wk post-parturition), also display susceptibility to tolerance induction. The results demonstrate that at 7-10 days after birth, when over 90% of the DNP-specific splenic B cells are resistant to tolerance induction, the majority of PC-specific B cells are tolerizable. These results re-emphasize tolerance susceptibility as a characteristic of developing clones, confirm the late acquisition of PC-specific B cells, and support the contention that the acquisition of the specificity repertoire is a highly ordered, specifically predetermined process which is independent of antigen-driven events.