HPLC法同时测定痤疮擦剂中3种组份的含量

目的：研究测定痤疮擦中甲硝唑,水杨酸及氯霉素的含量,方法：采用反相高效应相法,外标法,shin-packCLC-ODS柱,流动相为甲醇－水（50：50）,检测波长280nm,结果：一次进样即可完成3种不同组分的含量分析,线性范围分别是：甲硝唑20.28-104.4ug.ml^-1,r=0.9999,水杨酸80．2－401ug.ml^-1,r=0.9998,氯霉素40－200ug.ml^-1,r=0.9998,平均回收率,甲硝唑96.05%,RSD=1.45％,RSD=0.98%,氯霉素97．34％,RSD＝1．20％,结论：本法快速,筒便,准确,分离度好,可用于该制剂中3种成分的含量测定。     

HPLC法测定消炎止痒酊中氯霉素和地塞米松含量

目的：采用HPLC测定消炎止痒酊中氯霉素和地塞米松的含量，方法：采用C18柱，流动相为甲醇－水（75：25v/v).检测波长氯毒素为278nm，地塞米松240nm，结果：氯霉素在100－500ug.ml^-1浓度范围内，回归方程A＝27614＋4627C,r=0.997,回收率98．9％，RSD＝0．43％，地塞米松在30－150ug.ml^-1浓度范围内，峰面积与浓度呈线性关系，回归方程A＝4092＋20148C，r=0.9998，回收率99．1％，RSD＝0．38％，结论；该方法可用于消炎止痒酊中氯霉素和地塞米松的含量测定。     

HPLC法测定复方替硝唑漱口液中替硝唑和呋喃西林的含量

目的：建立测定复方替硝唑嗽口液中替硝唑，呋喃西林含量的高效液相色谱法，方法：采用反相ODS色谱柱，水－乙腈－冰醋酸（85：15：0．1）为流动相，检测波长为317nm，结果：替硝唑和呋喃西林的线性范围分别为50－350ug.ml^-1(r=0.9996),10-70(ug.ml^-1)(r=0.9997)，加可咽收率分别为100．56％，RSD＝0.69%,100.64%,RSD=0.79%,日内RSD为0.12%-1.35%和0.66%-1.45%，n=6,日间RSD为0.28%-1.39%，和0.18%-1.23%,n=5，结论：HPLC法可用于复方替硝唑漱口液的含量定，方法准确，灵敏，专属性强。     

HPLC法测定复方氯霉素洗剂中氯霉素和水杨酸的含量

目的建立高效液相色谱法测定复方氯霉素洗剂中氯霉素和水杨酸含量。方法色谱柱为C18（150mm×3．9mm，5μm）；流动相为磷酸盐缓冲液（0．01mmol·L^-1，pH3．5）-甲醇（55：45）；流速为1．0ml／min；检测波长为278nm；柱温为25℃。结果氯霉素、水杨酸分别在4．08～40．8μg·ml^-1（r=0．9989）、7．76～77．6μg·ml^-1（r=0．9990）范围内呈良好的线性关系，平均回收率分别为100．5％、100．1％，RSD分别为0．52％、0．66％（n=9）。结论本方法简便、准确、快速、重现性好，可用于该制剂的含量测定。     

高效液相色谱法测定大鼠血清中艾司洛尔的含量

目的：建立高效液相色谱法（HPLC）测定因清中艾司洛尔浓度的方法。方法：以甲醇－冰醋酸－0．2％醋酸钠（60：1：40）为流动相，流速1．0ml/min，检测波长为280nm。结果：在10－600ug/ml^-1范围内样品色谱峰高与血清药物浓度呈良好的线性关系，r=0.9998，日内及日内RSD分别测得为3．05％,4．43％，平均加样回收率为100．45％，血清中药物最低检测浓度为13．3ug.L^-1。结论：本方法适于艾司洛尔的血药浓度监测。     

HPLC法同时测定复方氯霉素醇溶液中氯霉素和水杨酸含量

目的建立HPLC法同时测定复方氯霉素醇溶液中氯霉素和水杨酸含量。方法采用CAPCELL PAK C8DD（4.6mm×250mm,5μm）色谱柱;流动相：甲醇-0.05mol.L^-1磷酸二氢钠溶液（用磷酸调pH至2.5）（29∶71）;流速：1.0ml/m in;检测波长278nm;柱温：40℃;进样量10μl。结果氯霉素、水杨酸的线性范围分别为0.020 54-1.027mg.ml^-1、0.021-1.05mg.ml^-1（r=0.999 9）,平均回收率分别为100.01%、99.85%,RSD分别为0.9%、1.0%（n=9）。结论本方法快速、准确,可同时测定复方氯霉素醇溶液中两种主要成分的含量。可用于复方氯霉素醇溶液的质量控制。     

复方氯霉素醇溶液含量测定方法的改进

目的 建立复方氯霉素醇溶液中水杨酸和氯霉素的含量测定方法.方法 采用反相高效液相色谱法,色谱柱：C18（250mm×4.6mm,5μm）;流动相：甲醇-水-冰醋酸（50∶50∶0.1）;流速：0.8ml/min;检测波长：295nm;柱温：30℃.结果 水杨酸在0.253 5～2.535 0 μg·ml^-1范围内呈良好线性关系,r=0.999 9,平均回收率99.75%,RSD为1.3%;氯霉素在0.255 0～2.550 0 μg·ml^-1范围内呈良好线性关系,r=0.999 9,平均回收率99.18%,RSD为1.3%.结论 方法简便、准确,重现性好.     

HPLC法测定滴鼻灵中地塞米松磷酸钠的含量

目的：建立HPLC法测定滴鼻灵中地塞米松磷酸钠的含量,方法：以十八烷基硅烷键合硅胶为填充剂,0．01mol.L^-1磷酸二氢钾（KH2PO4）－甲醇－磷酸（48：52：0．4）为流动相,检测波长240nm。结果：地塞米松磷酸钠在1．26－10．08ug/ml^-1(r=0.9999)范围内线性关系良好。平均回收率为99．16％,RSD为0．65％（n=6)。结论：本方法快速,准确,可用于测定滴鼻灵中地塞米松磷酸钠的含量。     

HPLC法测定急麻颗粒剂中黄芩苷的含量

目的：本应用HPHC法浊定麻颗粒剂中黄芩苷的含量,方法：色谱柱ODS,流动相为甲醇－磷酸液（0．4％）（60：40）,检测波长为280nm,结果：含量测定结果为1.551mg.g^-1(n=3),线性关系范围,黄芩苷在0.5-4ug范围内线关系良好（r=0.9993),平均回收率为98．43％,RSD为1．11％（n=6),结论：该法操作简便,快速,可用于该药中黄芩苷的含量测定。     

分光光度法测定复方醋酸曲安奈德搽剂中酯酸曲安奈德的含量

目的：测定复方醋酸曲安奈德搽剂中醋酸曲安奈德的含量，方法：采用分光光度法，测定波长为485nm，结果：醋酸曲安奈德在4．22－33.74ug.ml^-1(r=0.9998)范围内，吸收度值与其浓廑良好的线性关系，方法平均回收率为99．82％，RSD为0．84％，（n=6)。结论：方法简便易行，快速，准确，可做为该制剂的质量控制方法。     

The role of interventional radiology in trauma

Introduction Interventional Radiology has evolved into a specialty having enormous input into the care of the traumatized patient.In all hospitals,regardless of size,the Interventional Radiologist must consider their relationships with the trauma service in order to     

Diagnóstico ecográfico del neumotórax

The ultrasonographic diagnosis of pneumothorax is based on the analysis of artifacts. It is possible to confirm or rule out pneumothorax by combining the following signs: lung sliding, the A and B lines, and the lung point. One fundamental advantage of lung ultrasonography is its easy access in any critical situation, especially in patients in the intensive care unit. For this reason, chest ultrasonography can be used as an alternative to plain-film X-rays and computed tomography in critical patients and in patients with normal plain films in whom pneumothorax is strongly suspected, as well as to evaluate the extent of the pneumothorax and monitor its evolution.     

Carbohydrate Supplementation during Exercise: Does It Help? How Much is Too Much?

KEY POINTS· Carbohydrate intake during exercise can delay the onset of fatigue and improve performance of prolonged exercise as well as exercise of shorter duration and greater intensity (e.g., continuous exercise lasting about 1h and intermittent high-intensity exercise), but the mechanisms by which performance is improved are different.     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

磁共振DWI和动态增强鉴别颅底脊索瘤和侵袭性垂体瘤的应用

目的 探讨磁共振扩散加权成像(DWI)和动态增强在颅底脊索瘤和侵袭性垂体瘤(IPA)鉴别诊断中的应用价值.方法 搜集经手术病理证实且影像学有鞍区破坏的颅底脊索瘤患者15例、向鼻咽部侵犯的IPA患者20例.测量二者的表观扩散系数(ADC)值,绘制受试者工作特征曲线(ROC),分析动态增强曲线的类型,统计达峰时间(TTP)、增强峰值(EP)和最大对比增强率(MCER),分析各个参数在鉴别诊断中的价值.结果 颅底脊索瘤的ADC值为(1.274±0.07)×10-3mm2/s,高于IPA ADC值(0.672±0.03) ×10-3 mm2/s(P ＜0.001),ADC阈值为0.964×10-3mm2/s时,ROC曲线下面积为0.997,敏感度为93.3％,特异度为100％.颅底脊索瘤时间-信号强度曲线(TIC)Ⅰ型14例,TICⅢ型1例,此例TICⅢ型者TTP约40 s;IPA TIC Ⅰ型7例,TICⅢ型13例.颅底脊索瘤和IPA的EP、MCER差异均有统计学意义(P ＜0.001).结论 ADC值和TIC的类型及其相关参数(EP,MCER)有助于颅底脊索瘤和IPA之间的鉴别.     

Do Balance Board Training Programs Reduce the Risk of Ankle Sprains in Athletes?

Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).     

Treatment of intrahepatic arteriovenous fistula in hepatocellular carcinoma with N-butyl-2-cyanoacrylate

Primary hepatocellular carcinoma (HCC) continues to be one of the most common malignancies with an incidence of approximately one million cases per year and a dismal prognosis; some authors have reported a median survival of 1 ～ 2 months after diagnosis. Although surgery remains the only hope for cure, few patients are candidates[1,2].     

U50,488H对正常及缺氧心肌细胞L型钙电流的作用

目的 探讨心脏阿片受体和β-受体相互作用的机制。方法 采用全细胞膜片钳技术,观察U50,488H(β-阿片受体选择性激动剂)对正常和缺氧心肌细胞L型钙电流的作用。结果 U50,488H剂量依赖性(0．1～100μmol／L)抑制正常心肌细胞的L型钙电流及异丙肾上腺素(0．1μmol／L)激动的钙电流,而细胞缺氧后,这一抑制作用减弱;U50,488H对Forskolin(10μmol／L)激动的L型钙电流无明显影响。结论 β-阿片受体对β-受体信号的负性调节作用在细胞缺氧后减弱,其作用位点可能发生于β-受体与腺苷酸环化酶环节之间。