精制抗五步蛇毒血清和抗蝮蛇毒血清对竹叶青蛇毒的中和作用

本文通过琼脂免疫扩散实验表明抗五步蛇毒血清和抗蝮蛇毒血清对竹叶青蛇毒有中和作用，对竹叶青蛇毒的出血毒素及促凝毒性作用两种抗血清均有中和及对抗作用；两种抗血清的混和液对竹叶青蛇毒有协同作用。     

抗蛇毒血清的应用与并发症处理

自抗蛇毒血清发明以来,经100多年的试验和改进,抗蛇毒血清已公认为治疗蛇伤的最有效药物.抗蛇毒血清的作用机理,是抗蛇毒血清中的抗体与相应蛇种的蛇毒结合,可形成无毒性的抗原抗体复合物,起中和解毒作用.抗蛇毒血清目前已成为治疗毒蛇咬伤的首选特效药物.上海赛伦生物技术公司认为:"毒蛇咬伤6～8小时内,应用抗蛇毒血清,96%以上病例在1～2天即可治愈出院."目前我国已能生产抗银环蛇毒血清、抗蝮蛇毒血清、抗眼镜蛇毒血清、抗五步蛇毒血清、抗蝰蛇毒血清、抗金环蛇毒血清等6种(其治疗剂量参考附表).单价血清只能中和同种蛇毒,多价血清能治疗多种蛇毒,就效价来说多价不如单价.     

精制抗眼镜蛇毒血清与精制抗银环蛇毒血清并用治疗眼镜王蛇伤的疗效评价

国内至今研制成功的六种单价抗蛇毒血清中尚无抗眼镜王蛇毒血清。眼镜王蛇伤在所有毒蛇伤中占的比例虽低，但其死亡率极高。本文收集9例危重眼镜王蛇伤患者的治疗并结合部份基础研究，对眼镜王蛇伤患者合用抗眼镜蛇毒血清、抗银环蛇毒血清治疗的疗效作一分析，证实抗眼镜蛇毒血清与抗银环蛇毒血清合用治疗眼镜王蛇伤疗效较佳。     

广西眼镜蛇毒对人类小涎腺腺样囊性癌细胞株的抑制作用

目的研究广西眼镜蛇毒（guangxi cobravenom）对人类小涎腺腺样囊性癌（NACC）细胞在体外生长的抑制作用。方法以不同浓度的眼镜蛇毒作用于NACC细胞，应用三磷酸腺苷一生物荧光肿瘤化疗药物敏感试验法（ATP—TCA法）测定细胞的增殖状态；应用细胞形态学和流式细胞仪观察细胞凋亡。结果实验显示眼镜蛇毒对体外培养的NACC有明显的抑制作用，与浓度和作用时间呈正比。用浓度2．0μg／mL的眼镜蛇毒处理癌细胞48h后，试验组细胞凋亡率显著高于对照组。HE染色观察到眼镜蛇毒作用NACC细胞后其形态发生改变。结论眼镜蛇毒能抑制NACC细胞增殖并诱导其凋亡。     

抢救眼镜王蛇咬伤2例报告

本文报告２例眼镜王蛇咬伤的危重型患者，成功１例。作者强调采取下列措施：（１）尽快足量应用抗眼镜蛇毒血清和抗银环蛇毒血清，（２）及时切开气管确保呼吸道通畅；（３）切开静脉输液：（４）中心静脉压监护，（５）中药的应用。     

五步蛇和蝮蛇的抗蛇毒血清对烙铁头蛇毒的中和作用

针对我国院目前尚无烙铁头蛇毒血清，本研究旨在利用国内现有的精制抗五步蛇毒血清和精制抗蝮蛇毒血清，通过动物实验，证明这两种抗血清在体外和体内都能中和烙铁头蛇毒，对被烙铁头蛇毒中毒的小鼠有明显保护作用，且两种血清的保护率无显著差异。     

蛇毒对人及小鼠传代细胞的杀伤作用

本文应用体外微量培养方法,测定9种蛇毒(眼镜王蛇、眼镜蛇、金环蛇、银环蛇、青环海蛇、蝮蛇、尖吻蝮,竹叶青及圆斑蝰)对4株人体肿瘤细胞系(鼻咽癌、肝癌、子宫颈癌及胃癌)和2株小鼠传代细咆(骨髓瘤细胞及小鼠肥大细胞)的杀伤作用和抑制生长作用。结果表明眼镜蛇毒对肿瘤细胞的杀伤作用最强。胃癌及鼻咽癌细胞株对多种蛇毒呈现较高的敏感性。     

蛇毒组分对K562阿霉素敏感株和耐药株的抑制作用

目的：通过对中华眼镜蛇毒进行分离和各组分的初筛,寻找逆转K562对阿霉素耐药的活性成分KD-Ⅲ-1．为今后研究肿瘤耐药性奠定工作基础。方法：通过凝胶分离得到的蛇毒组分分别作用于K562对阿霉素耐药株K562／A和敏感株K562／S,筛选有效活性组分;通过荧光探针Rhl23测定P-gP蛋白活性和PI染色进一步确定该组分的逆转K562／A的耐药活性。结果：分别给予2μg／mL阿霉素（Adr）和各浓度蛇毒组分处理24h后,可以发现蛇毒组分对阿霉素敏感株K562／S和耐药株K562／A都有明显的抑制作用,并呈现出剂量-效应关系。1μg／mL蛇毒组分处理组与2μg/mL蛇毒处理组抑制作用明显;在药物持续作用48h后对K562／A的活性仍有抑制作用在0．5、1、2μg／mL的蛇毒组分组表现的更加明显。通过Rho外排实验发现蛇毒粗毒组平均荧光强度（MFI）与阴性对照组没有明显差异,而2．5μg／mL蛇毒组分组的MFI明显降低,与对照组相比具有统计学意义（P〈0．05）。2．5μg／mL蛇毒粗毒和分离组分分别对K562／S敏感株和K562／A耐药株作用3h后。K562／S的PI染色阳性率明显升高,而K562／A的PI染色阳性率并没有明显升高。结论：蛇毒组分KD-Ⅲ-1对K562／A和K562／S细胞均有明显抑制的作用,抑制作用可能与诱导凋亡有关。     

精制抗银环蛇毒血清、胰蛋白酶及糜蛋白酶对银环蛇毒中毒动物保护作用的比较

本文根据动物实验观察,报导精制抗银环蛇毒血清、胰蛋白酶及糜蛋白酶对银环蛇毒中毒动物保护作用的比较,为临床合理选用或合用这些药物提供参考。一、实验材料(一) 银环蛇毒(下文简称蛇毒);本教研室自行采集。(二) 精制抗银环蛇毒血清(下文简称抗血清):本教研室与上海生物制品研究所协作研制。(三) 胰蛋白酶:上海生物化学制药厂生产供注射用结晶胰蛋白酶,每一安瓿为2,000u。(四) 糜蛋白酶:上海生物化学制药厂生产供注射用结晶糜蛋白酶,每一安瓿5mg。(五) 实验动物:体重18～22g小白鼠,雌雄兼用。二、实验方法将蛇毒结晶用蒸馏水深解配成1 mg/1ml母液,按实验需要再配成3.5 μg/0.1 ml、7μg/0.1 ml、10.5μg/0.1ml及14μg/0.1ml四种蛇毒溶液(这批蛇     

中国眼镜蛇毒对血小板聚集的影响

中国常见八种蛇毒包括眼镜蛇毒;银环蛇毒;金环蛇毒;眼镜王蛇毒;蝰蛇毒;蝮蛇毒;竹叶青蛇毒;五步蛇毒。在体外前四种蛇毒通过ADP的诱导,抑制家兔血小板的聚集,后四个毒促进家兔血小板聚集。用CM-Sephaex C 50柱层析3.0×65cm。眼镜蛇毒分为9个组份。组份Ⅰ和组份Ⅱ有明显抑制血小板聚集作用。组份I和组份Ⅱ经聚丙烯酰胺凝胶园盘电泳,鉴定为一区带。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.