HPLC法测定盐酸普鲁卡因注射液中分解产物对氨基苯甲酸含量

目的：研究和测定盐酸普鲁卡因注射液中的降解产物对氨基甲酸,方法：采用离子对ＨＰＬＣ法,固定相Ｌｕｎａ５μＣ１８（２）柱,流动相为缓冲液（０．００５ｍｏｌ．Ｌ＾－１磷酸二氢钾,ｐＨ３．０,０．１％庚烷磺酸钠）－甲醇（７７：２３）;流速１．５ｍｌ／ｍｉｎ,柱温３５℃;检测波长２８７ｎｍ,结果：对氨基苯酸浓度在２．０～２０．０μｇ．ｍｌ＾－１范围,线性关系良好,回归方程为Ｃ＝５．８０４１×１０＾－５＋０．０７８２９,ｒ ＝０．９９９９。回收率为１００．２％,ＲＳＤ＝０．３２％,结论：本测定方法简便快速,结果准确,可用于该制剂有关物质检查。     

介绍一种大鼠脑中γ—氨基丁酸受体的检测方法

目的：介绍一种大鼠脑中γ＝氨基丁酸本的快速简便的检测方法。方法：用＾３Ｈ－ＧＡＢＡ为配体来检测大鼠脑神经末梢微囊中的ＧＡＢＡ受体。检测ＧＡＢＡ受体的合适条件是１．０×１０＾－３ｍｏｌ／Ｌ的脑膜蛋白在３７℃下反应３０ｍｉｎ。结果：大鼠脑中存在ＧＡＢＡ受体,Ｋｄ＝（９７．３±５．８）×１０＾－９ｍｏｌ／ｇ蛋白±０．０１０３×１０＾－９ｍｏｌ／ｇ蛋白,属低亲和力受体。结论：用＾３Ｈ－ＧＡＢＡ可以检测大鼠     

人外周血林巴细胞松果体素受体鉴定及意义

为证明免疫组织上是否存在松果体素受体（ＭＲ）。以＾１２５Ｉ－松果体系（Ｍ）为配体，应用放射配体结合法检测４０例健康人外周血淋巴细胞＾１２５Ｉ－Ｍ特异结合位点。结果：①＾１３５Ｉ－Ｍ特异结合最大结合容量（Ｂｍａｘ）为０．３６±０．１０ｆｍｏｌ／１０＾６细胞（２１７±６０位点／细胞）；平衡解离常数（Ｋｄ）为７９±１３ｐｍｏｌ／Ｌ；②动力学分析显示Ｋ１＝（１．２４±０．３１）×１０＾－７Ｌ·ｍｉｎ＾－１     

核工业职工在危险及相关职业危害的分析

目的 在核工业职工全死因调查的基础上，着重进行死因危险及相关职业危害的分析，探讨职业与健康的关系。方法 调查对象为１１个单位自组建至１９９０年底在册职工、分组观察。调查历年职工人数及死亡资料，按ＩＣＤ－９原则进行死因统计。结果 粗死亡率１９３．６６×１０＾－５，标化死亡率２３７．６５×１０＾－５，而放射组、非放射组分别为３４６．１１×１０＾－５、２０２．０３×１０＾－５，但均低于全国值。放射组与非     

RP—HPLC法测定复方氧氟沙星栓剂中氧氟沙星和甲硝唑的含量

目的：建立反相高效液相色谱法,测定复方氧氟沙星栓剂中氧氟沙星和甲硝唑的含量。方法：采用ＯＤＳ－Ｃ１８（４．６×２５０ｍｍ）５μｍ不锈钢柱,以盐酸雷尼替丁为内标物,甲醇：水：磷酸：乙乙胺（３０：７０：０．３９：０．７０;ｐＨ＝３．１５）为流动相,检测波长２７５ｎｍ。结果：氧氟沙星在３．０￣２６．０μｇ·ｍｌ＾－１范围内线性关系良好,ｒ＝０．９９９９（ｎ＝５）,平均回收率为９８．８２％,ＲＳＤ＝０．７     

RP—HPLC法测定人血清中醋酸甲地孕酮浓度

目的：建立高效液相色谱法测定人血清中醛酸甲地孕酮浓度。方法：分析柱为ＹＷＧ－Ｃ１８（１０μｍ,４．６ｍｍ×２５０ｍｍ）;流动相为水－乙腈（４０：６０）;流速为１．０ｍｌ／ｍｉｎ;紫外检测波长为２８８ｎｍ;用乙酸乙酯作为提取液。结果：血清中醛酸甲地孕酮浓度在１￣１２８ｎｇ·ｍｌ＾－１范围内线性关系良好（ｒ＝０．９９９７）;醛酸甲地孕酮高、中、低３个浓度的日内、日间变异系数分别小于１０％和１￣１２８ｎ     

高效毛细管电泳法测定何首乌中二苯乙烯甙的含量

目的：建立测定何首乌中二苯乙烯甙含量的高效毛细管电泳法。方法：电解液为２０ｍｍｏｌ·Ｌ＾－１硼砂,检测波长２１４ｎｍ,二苯乙烯甙为对照品,测定了何首乌及其制剂中二苯乙烯甙的含量。结果：进样量在１０．０￣１００．０μｇ·ｍｌ＾－１范围内线性关系良好（ｒ＝０．０９９９９）,加样回收率为９８．７１％,ＲＳＤ为１．５１％（ｎ＝３）,测得生何首乌,制何首乌及优生宝胶囊中二苯乙烯甙的含量分别为０．１２７％、０     

IL—2受体放射配基结合分析法的建立

为建立一种稳定可靠的白细胞介素－２受体（ＩＬ－２Ｒ）放射配合基结合分析（ＲＢＡ）法，对６例健康男性取血分离井培养外周血单个核细胞（ＰＢＭＣ），进行ＩＬ－２Ｒ多点法；１０例健康男性进行ＰＢＭＣＩＬ－２Ｒ单点法ＲＢＡ。结果：多点法ＲＢＡ测得每个细胞高亲和力ＩＬ－２Ｒ的数量为１１２５．７±１８０．４个，其Ｋｄ高＝（７．０６０±２．９２８）×１０＾－１１ｍｏｌ／Ｌ；低亲和力ＩＬ－２Ｒ的数量为１２５２４．５     

高效液相色谱法测定法莫替丁含量

目的：高效液相色谱法测定法莫替丁的含量。方法：固定相ＣＬＯ－ＯＤＳ ５μｍ（色柱４．６×１５０ｍｍ）,流动相：甲醇－磷酸盐缓冲液（２０：８０）,以４－氨基安替比林为内标,检测波长为２６８ｎｍ。结果：在２０￣１００μｇ·ｍｌ＾－１范围内,线性关系良好,其相关系数ｒ＝０．９９９８。平均回收率为９９．８６％,ＲＳＤ为０．８６％（ｎ＝４）,结论：本法简单快速,可用于法莫替丁的定量分析。     

紫外分光光度法测定盐酸丁卡因胶浆中盐酸丁卡因的含量

目的：建立测定盐酸丁卡因胶浆中盐酸丁卡因含量的方法。方法：采用紫外分光光度法。结果：盐酸丁卡因的线性范围为５ ～１０μｇ·ｍｌ－１ ,Ａ＝０ ．０７５１２Ｃ＋ ０．００３７１,ｒ ＝１．０００ ０。结论：此方法简便、快速、经济、准确、适用于药品质量控制。     

The role of interventional radiology in trauma

Introduction Interventional Radiology has evolved into a specialty having enormous input into the care of the traumatized patient.In all hospitals,regardless of size,the Interventional Radiologist must consider their relationships with the trauma service in order to     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

Do Balance Board Training Programs Reduce the Risk of Ankle Sprains in Athletes?

Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).     

Carbohydrate Supplementation during Exercise: Does It Help? How Much is Too Much?

KEY POINTS· Carbohydrate intake during exercise can delay the onset of fatigue and improve performance of prolonged exercise as well as exercise of shorter duration and greater intensity (e.g., continuous exercise lasting about 1h and intermittent high-intensity exercise), but the mechanisms by which performance is improved are different.     

Diagnóstico ecográfico del neumotórax

The ultrasonographic diagnosis of pneumothorax is based on the analysis of artifacts. It is possible to confirm or rule out pneumothorax by combining the following signs: lung sliding, the A and B lines, and the lung point. One fundamental advantage of lung ultrasonography is its easy access in any critical situation, especially in patients in the intensive care unit. For this reason, chest ultrasonography can be used as an alternative to plain-film X-rays and computed tomography in critical patients and in patients with normal plain films in whom pneumothorax is strongly suspected, as well as to evaluate the extent of the pneumothorax and monitor its evolution.     

High Intensity Interval Training:New Insights

KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief，intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.，≥90%of VO2 peak).     

磁共振DWI和动态增强鉴别颅底脊索瘤和侵袭性垂体瘤的应用

目的 探讨磁共振扩散加权成像(DWI)和动态增强在颅底脊索瘤和侵袭性垂体瘤(IPA)鉴别诊断中的应用价值.方法 搜集经手术病理证实且影像学有鞍区破坏的颅底脊索瘤患者15例、向鼻咽部侵犯的IPA患者20例.测量二者的表观扩散系数(ADC)值,绘制受试者工作特征曲线(ROC),分析动态增强曲线的类型,统计达峰时间(TTP)、增强峰值(EP)和最大对比增强率(MCER),分析各个参数在鉴别诊断中的价值.结果 颅底脊索瘤的ADC值为(1.274±0.07)×10-3mm2/s,高于IPA ADC值(0.672±0.03) ×10-3 mm2/s(P ＜0.001),ADC阈值为0.964×10-3mm2/s时,ROC曲线下面积为0.997,敏感度为93.3％,特异度为100％.颅底脊索瘤时间-信号强度曲线(TIC)Ⅰ型14例,TICⅢ型1例,此例TICⅢ型者TTP约40 s;IPA TIC Ⅰ型7例,TICⅢ型13例.颅底脊索瘤和IPA的EP、MCER差异均有统计学意义(P ＜0.001).结论 ADC值和TIC的类型及其相关参数(EP,MCER)有助于颅底脊索瘤和IPA之间的鉴别.     

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version

The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.