快速检测神经元放电串中优势模式

目的：新建一个“优势模式（ｆａｖｏｒｅｄ ｐａｔｔｅｒｎ，ＦＰ）”快速检测法，用以检测不同神经元放电串中之ＦＰ，并检验其效果。方法：在原有的“改进的ＦＰ检测法”的基础上，采用数种措施实现快速检测，并用多个指标检验其检测效果。结果：（１）具有３种不同程度变动的各种模拟ＦＰ的检出阈均为２，表明该法敏感；（２）自发放电串中各个ＦＰ重复出现时，其全长和所含不同放电间隔长度之变异系数分别为５％和１７％，均小于检测所规定的容差，表明该法可靠；（３）自发放电串中ＦＰ长时间稳定地存在，而诱发放电串中不同ＦＰ的总数随放电率的变化而增减；（４）所有被检测的放电串（总数９２）都能检测到ＦＰ，检测一个１２００～１５００个动作电位的放电串中的ＦＰ仅需２～３ｓ。结论：新建立的“快速ＦＰ检测法”可能是研究神经信息编码的一个很有用的手段。     

褪黑激素对大鼠弓状核神经元自发单位放电的调制作用

[目的]研究褪黑激素(MEL)对大鼠下丘脑弓状核(ARC)神经元自发单位放电活动的影响。[方法]记录ARC神经元自发单位放电,微电泳注射MEL。分析注射MEL前和注射MEL时ARC神经元自发单位放电的放电间隔(ISI)及其变化率。[结果]微电泳MEL时,220个ARC神经元自发单位放电活动的变化有3种情况:92个单位(41.82％)的ISI减小(兴奋);61个单位(27.73％)的ISI增加(抑制);67个单位(30.45％)的ISI无明显改变(不反应)。[结论]MEL对ARC大部分神经元的自发放电活动有兴奋或抑制的调制作用,以兴奋作用为主,但对小部分神经元的自发放电活动无明显影响。     

静脉注射皮质酮对大鼠头端延髓腹外侧区前交感神经元的作用

目的 :研究糖皮质激素在交感神经系统和心血管活动调节中的作用。方法 :细胞外记录头端延髓腹外侧区 (RVL M)功能性质明确的前交感神经元自发放电 ;静脉注射皮质酮 (5 0、10 0、15 0μg/ kg)观察前交感神经元放电频率的变化。 结果 :静脉注射不同剂量皮质酮后 ,12个 RVL M前交感神经元的放电频率均较注射前明显增加 (P<0 .0 5 ) ,具有剂量依赖性 ,作用潜伏期为 (10 4± 2 5 ) s。 结论 :皮质酮能快速兴奋 RVL M前交感神经元 ,提示糖皮质激素可能通过“快速膜效应”参与交感神经系统和心血管活动的调节     

鞍钢某泳池水卫生指标检测结果分析

我站于 2 0 0 2年 7～ 9月间对鞍钢某游泳池水进行细菌总数、大肠菌群检测 ,现将结果报告如下。1　资料与方法1 1　样品来源　随机抽取游泳池水共十四份检样。1 2　检测项目与方法　检测项目 :细菌总数、大肠菌群。检测方法 :细菌总数、大肠菌群检测均按《卫生微生物检验方法》中规定之方法。判定标准为细菌总数≤ 10 0 0个 /ml。大肠菌群≤ 18个 /L为合格。1 3　结果　十四份样品中六份不合格、八份合格见表 1,表2。表 1　游泳池水检测结果样品编号 1 2 3 4 5 6 7 891 0 1 1 1 2 1 3 1 4细菌总数(个 /ml) 90 86 1 2 81 0 0 60 860 340 0 2…     

丘脑底核的电生理特性及刺激在帕金森病中的应用研究

目的　利用FHC微电极记录仪对丘脑底核 (STN)的细胞放电进行记录 ,研究其细胞放电的模式和电生理特点以及在帕金森病 (PD)定向手术治疗中的定位作用 ;利用植入性刺激电极植入至STN核团内 ,研究脑深部刺激(DBS)对PD的治疗作用 ,观察术中丘脑底核刺激对PD的震颤、肌僵直、运动缓慢的缓解效果以及对语言的影响 ,探讨电极在丘脑底核内的解剖位置与刺激效果的关系 ,以寻找植入性刺激电极在STN内的最佳位置。方法　 2 6例帕金森病患者 ,利用MRI影像导向的立体定向方法将微电极植入丘脑底核 ,其解剖靶点 :X =1 1～ 1 3mm ,Y =-1～ -3mm ,Z =-7mm。记录从靶点上 1 0mm开始 ,进行连续记录直至靶点下 3mm(黑质网状区 )。将植入性刺激电极植入至STN核团内并给予高频刺激 (频率 1 3 0～ 1 5 0Hz,脉宽90～ 1 5 0 μs,电压自 0 .5V开始 ,逐渐增至 8V) ;利用电极的不同触点分别进行刺激 ,根据刺激效果和副反应的出现情况 ,确定电极在STN中的最佳位置。其中有 3例进行了STN电极永久性植入慢性电刺激 ,术后随访 6～2 3个月。结果　 2 6例术中微电极记录发现 ,未定带区其细胞放电呈现为规则节律的低幅放电 ,STN核团内可记录到高频的紧张性放电 (3 5± 1 3 .3 )Hz、爆发性单细胞放电和簇状放电三种类型的细胞放电。黑质 (SN     

三种梅毒血清学试验检测方法的比较

目的：比较目前临床常用的三种梅毒血清学试验方法的效果。方法：以明胶颗粒凝集法（TPPA）为标准，对甲苯胺红不加热试验（TRUST）与梅毒快速检测法（The SD BIOLINE Syphilis 3．0 test）两种方法的检测结果进行比较。结果：两种方法的检测结果有显著性差异（P〈0．05），其灵敏度明显低于TPPA法；而梅毒快速检测法（The SD BIOLINE Syphilis 3．0test）则与TPPA法结果之间无显著差异（P〈0．05），且梅毒快速检测法具有快速、准确的特点。结论：梅毒快速检测法是临床上进行血清梅毒检测较直接快速的检验筛查方法之一，可用于各级医疗卫生单位进行梅毒的日常筛查诊断。     

四种检验方法诊断细菌性阴道炎效果分析

目的探讨细菌性阴道炎(BV)诊断中不同检验方法检出效果的对比分析。方法选取2015年1月~2015年5月在我院妇产科门诊收治的120例BV患者,对120例患者阴道分泌物分别行BV快速检测、乳酸杆菌检测法、pH检测和胺试验检测比较分析四种BV检测方法的检出率。结果 BV快速检测法检出率为85.83%,乳酸杆菌检测法的检出率为67.50%,pH检测检出率为65.00%,胺试验检测检出率为62.50%,BV快速检测法检出率高于乳酸杆菌检测法、pH检测和胺试验检测,差异均具有统计学意义(P<0.001)。结论 BV快速检测的方法具有准确、迅速、操作过程简单的特点,可作为检验BV的首选方法,值得在临床上推广应用。     

1250例疑似细菌性阴道病患者阴道分泌物检测

目的探讨生化检测法在细菌性阴道病诊断中的价值。方法对妇科门诊1250例患者阴道分泌物进行常规及生化检测。结果在1250例疑似细菌性阴道病患者中,以“唾液酸苷酶阳性＋过氧化氢阳性”为诊断标准,共检出细菌性阴道病患者921例,占总数的73．68％;Amsel法检出细菌性阴道病患者897例,占总数的71．76％。生化检测法与Amsel法检出细菌性阴道病阳性率的差异无统计学意义（x2=0．76,P〉0．05）,二者的符合率为91．04％（1138／1250）,具有很高的一致性。结论生化检测准确、快速,具有较高的临床应用价值。     

生物芯片技术与Clearview快检在CT检测中的结果分析

目的 :对生物芯片技术与Clearview男女共用衣原体快速检测法检测沙眼衣原体感染结果比较。方法 :35 0例患者标本均来自本院性病门诊的随机疑似标本。结果 :血清生物芯片法阳性率 2 8.9% ;尿、分泌物Clearview男女共用衣原体快速检测法阳性率 2 7.1% ,两种结果基本吻合 (P <0 .0 1)。其中 6例生物芯片法检测为阳性结果 ,而尿、分泌物Clearview快检阴性 ;另有 2例患者Clearview快检阳性结果生物芯片阴性。结论 :生物芯片技术作为一种新技术、新方法 ,在沙眼衣原体 (CT)检测中有其不可比拟的特异性和灵敏度、准确率 ,是临床早期检测沙眼衣原体感染的一种敏感的抗体检测指标 ,与Clearview快检的抗原检测结果有很好的相关性。     

下丘脑背内侧核注射纳洛酮对束旁核痛兴奋单位放电的影响

用玻璃微电极记录大鼠丘脑束旁核痛兴奋单位(PfPE)放电。观察到下丘脑背内侧核(DMH)微量注射纳洛酮(Nx)后:(1)PfPE的自发和痛诱发放电频率增加,时程延长;(2)部份PfPE的自发放电类型由散在单脉冲变成散在间簇状或完全的簇状发放,而窟诱发放电类型由持续性发放转变为间断性暴发式放电。本实验提示:DMH局部的内源性阿片肽对PfPE放电活动有抑制性调控作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.