可溶性虫卵抗原淋巴细胞转化试验在日本血吸虫病诊断与疗效考核上的价值

本文报道急性、慢性早期和晚期日本血吸虫病患者120例的可溶性虫卵抗原(Soluble egg antigen)淋巴细胞转化试验(简称SEA淋转试验)。另以121例正常人及37例非血吸虫病患者作对照。SEA淋转试验的正常值范围为0～3.2％,大于4％时提示有血吸虫感染的可能。SEA淋转试验的灵敏性高,与环卵沉淀(Circumoval precipitin)试验(简称COP试验)合用时可互补不足。对疗效考核亦有一定的参考价值。     

石蜡包埋组织切片卵内沉淀反应诊断日本血吸虫病

本文国内首次报告用石蜡包埋含有大量血吸虫卵肉芽肿的肝脏组织切片卵内沉淀反应(IOP)技术试验人和动物血清115例,其中64例确诊为血吸虫病血清.62例(96.9%)呈阳性反应.正常人与动物血清40例、肺吸虫病人血清11例均呈阴性.这表明HOP试验对探测血吸虫病特异抗体具有较高的敏感性和特异性. 该115例血清作IOP与环卵沉淀反应(COP)比较试验.前者对血吸虫病诊断的敏感性、特异性与后者相似.IOP具有较多优点.不若COP需要分离纯卵和冻干等复杂技术,方法简便经济.因而可能成为一种较好的血吸虫病诊断的新技术.     

含血吸虫卵肝组织石蜡切片间接荧光抗体试验诊断日本血吸虫病

本文初步报告以含血吸虫卵肝组织石蜡切片进行间按荧光抗体(IFA)试验,并用干卵抗原间接血凝(IHA)试验作对比,检测了血清123份。其中89份为确诊血吸虫病人血清,IFA与IHA试验的粪检阳性符合率分别为95.5％和89.9％。但无显著差异(P>0.05),其余34名正常人血清,除做IFA与IHA试验外,还加做了环卵沉淀(COP)试验作比较,其假阳性率分别为5.9％(2/34)、2.9％(1/34)和2.9另(1/34),三者之间无显著差异(P>0.05),但IFA呈假阳性2名中1名IHA亦阳性,另1名COP亦阳性。上述结果表明:合血吸虫卵肝组织石蜡切片IFA试验对检测血吸虫病抗体具有较高的敏感性和特异性。而且切片来源丰富,制作简易。无需冰冻切片和低温保存等技术设备。固定在福尔马林液内含卵的肝脏或已制成的切片均可在室温内长期保持其抗原性,携带使用方便,较目前国内外IFA试验所用的血吸虫成虫冰冻防片或冰冻尾蚴为优。因而应用含血吸虫卵肝组织石蜡切片于IFA试验可成为诊断血吸虫病的新技术。     

环卵沉淀反应在日本血吸虫病临床诊断上的应用价值

血吸虫病的诊断,目前尚缺乏一简单而可靠的方法,Oliver-González 氏等(1954,1955,1956)发现曼氏血吸虫卵在感染曼氏血吸虫人、猴的血清中有环卵沉淀形成,对埃及血吸虫和日本血吸虫的抗血清也可有轻度反应,惟其他二种血吸虫卵对异性血清不发生反应,对其他寄生虫的抗血清三者均无交叉反应,不仅有临床诊断价值并可能作为疗效估价的辅助方法。我们在1957年春开始作环卵沉淀反应的家兔     

血吸虫病基本消灭地区应用间接血凝试验和环卵沉淀反应的诊断价值

1984年作者采用间接血凝试验(IHA)和环卵沉淀反应(COP)的综合查病方法,在武汉市郊区基本消灭血吸虫病地区进行诊断价值的考核。对曾经用常规粪检方法检查阴性的2037人群进行两项血清学试验的调查,结果双项阳性反应者35人,阳性率1.72％。两项血清学试验的阳性率与龚检复查阳性率加上治疗后抗体滴度升高的阳性率的对比,其阳性符合率高达90.33％。研究结果提示IHA和COP在基本消灭血吸虫病地区用于诊断病人可提高检出率,是简单易行,特异性和敏感性较高的方法。     

酶标记免疫吸附试验等四种免疫学方法诊断血吸虫病的研究

<正> 酶标记免疫吸附试验(ELISA)是近年来发展起来的一种新的免疫诊断技术。Huldt等(1975)用于诊断曼氏血吸虫病曾获得满意的结果。国内严自助等(1978)首先开展了本项研究,随后其它作者又陆续发表了多篇报告,一般认为它对血吸虫病的诊断有高度的敏感性,似优于环卵沉淀反应,且可用做流行病学调查。从1980年起我们应用本试验结合酶标记抗原对流免疫电泳试验(ELCIEP)、冻干血球间接血凝试验(IHA)和环卵沉淀反应(COP)四种方法,对经粪便沉淀孵化法查明的血吸虫病患者133例和非血吸虫病流行     

用福马林固定的组织切片来诊断血吸虫病的卵内沉淀(IOP)反应技术

近来发现,在血吸虫病免疫学诊断的环卵沉淀(COP)反应中涉及的虫卵抗原有显著的热稳定性,有时观察到在虫卵内沉淀(Kamiya,1980)。虫卵抗原的这个特性,通过福马林固定的日本血吸虫病鼠组织切片的使用,被成功地应用到诊断日本血吸虫病的间接荧光抗体试验(IFAT)中。而且这一结果又引起应用包埋在石腊中福马林固定组织切片的沉淀     

曼氏血吸虫病实验诊断的研究

本文用日本血吸虫成虫及虫卵抗原皮内试验、冻干虫卵环卵反应、尾蚴膜反应和粪便检查等方法调查124名援外人员,阳性率分别为25.9％、55.6％、57.2％和54.0％,属曼氏血吸虫感染。粪检阳性组的尾蚴膜和环卵反应阳性率分别达97.0％和94.0％。治疗后复查表明,环卵反应转阴率较尾蚴膜反应为高。作者还讨论了用日本血吸虫各期抗原检查曼氏血吸虫病病人及考核疗效的价值。     

血吸虫病基本消灭地区查病方案的探讨

<正> 我们于1986年9月,对已基本消灭血吸虫病的歙县桂林乡竦口村(1984～1985连续2年用尼龙绢袋粪便集卵孵化法九检清查应检人群的阳性率<1％,且当地未查获感染性钉螺)的五个相邻流行队,应用询问病史、皮内反应(IDT)、环卵沉淀反应(COP)、间接血凝试验(IHA)、肝脾触诊的综合查病方法,     

冰冻切片免疫酶染色法诊断血吸虫病的研究

用感染血吸虫鼠肝组织内虫卵冰冻切片作免疫酶染色试验,检测150例粪检阳性的血吸虫病人及150名健康人的血清,阳性率为96.7％,假阳性率为1.3‰;检测137例其它6种蠕虫病患者和50例内科疾病患者,交叉反应率为0～3.3％。该法的敏感性显著地高于用血吸虫成虫冰冻切片作免疫酶染色试验及环卵沉淀试验,而且方法简便,应用抗原经济,检测率高及获得结果较快,是一种较为理想的血吸虫病血清学诊断方法。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.