正交函数分光光度法测定复方甲基炔诺酮片中有效成分的含量

本文用正交函数分光光度法消除甲基炔诺酮和炔雌醇及辅料紫外吸收光谱的相互干扰,从而分别测得了复方甲基炔诺酮片两成分的含量。炔雌醇的回收率为101.4±0.7％,甲基炔诺酮的回收率为99.60±1.75％。     

－阶导数光谱法测定复方甲基炔诺酮片中甲基炔诺酮的含量

－阶导数光谱法测定复方甲基炔诺酮片中甲基炔诺酮的含量李荣生（全国计划生育药具质量南京监测中心，南京２１０００９）关键词甲基炔诺酮；含量测定；一阶导数法复方甲基炔诺酮片是一种常用的口服避孕药，主要成分是十八甲基炔诺酮（０．３０ｍｇ）和炔雌醇（０．０３ｍ...     

－阶导数光谱法测定复方甲基炔诺酮片中甲基炔诺酮的含量

－阶导数光谱法测定复方甲基炔诺酮片中甲基炔诺酮的含量李荣生（全国计划生育药具质量南京监测中心，南京２１０００９）关键词甲基炔诺酮；含量测定；一阶导数法复方甲基炔诺酮片是一种常用的口服避孕药，主要成分是十八甲基炔诺酮（０．３０ｍｇ）和炔雌醇（０．０３ｍ...     

荧光分光光度法测定复方甲基炔诺酮片中炔雌醇的含量

本文应用荧光分光光度法测定复方甲基炔诺酮片中炔雌醇的含量。方法系根据Kober反应的基本原理,采用混合试剂[硫酸-水(3:1) 0.5％(W/V)对苯二酚]激发荧光,荧光强度比较稳定,再现性较好,甲基炔诺酮和辅料基本不干扰,平均回收率的标准偏差低于4％。本法比过去的方法简便迅速且适用于单片分析。     

L-18甲基炔诺酮在阴道避孕环内剩余药量的高效液相色谱法测定

作者建立了快速简便的测定L-18甲基炔诺酮阴道避孕环中的18甲基炔诺酮剩余量的反相高效液相色谱(HPLC)分析法。采用YWG-C_(18)H_(37)柱,甲醇-水流动相,紫外分光光度法(UV)波长254nm检测器,使用炔诺酮作内标化合物。色谱分析前样品环先经切碎并以乙醇-氯仿提取。色谱峰分离完全,方法线性良好,平均回收率为99.98±1.70％(n=15)。本法应用于临床样品测定结果满意。     

八种避孕药致突变性研究

用微核试验和SOS试验检测八种避孕药的致突变性。结果表明，探亲避孕片1号，53号探亲抗孕片，口服避孕片1号，2号避孕片，0号避孕片，炔雌醇片和乖复方甲基炔诺酮片两种试验结果均为阴性。麦尔伊注射液微桉试验阳性，SOS试验可疑阳性。     

小儿误服复方18甲基炔诺酮2例

例1,幼女,3岁,因乳房肿大,阴道出血3 d就诊。其母诉于1997年误服复方18甲基炔诺酮6片（每片含18甲基炔诺酮12 mg,炔雌醚3 mg）,10 d后,家长在帮其洗澡时发现,两侧乳房丰满,阴道流血。体检：生长发育正常,两侧乳房隆起,乳晕色素沉着,乳头增大如黄豆,乳核可触及,女性外阴组织松软,小阴唇着色较深,阴道口有少量出血,未见其他第二性征发育。例2,幼女,4岁,恶心,两侧乳房丰满,阴道流血2 d就诊,母诉小孩见避孕药复方18甲基炔诺酮好看味甜,每天当糖丸吞服1～2片不等,共服5～6 d。体检：发育正常,皮下脂肪增多,心肺均无异…     

雌、孕激素对大鼠子宫内膜NO含量的影响

目的观察乙烯雌酚、左旋18甲基炔诺酮对SD大鼠子宫内膜NO含量的影响。方法实验分为对照组、雌激素组、孕激素组,采用Griess方法测定NO含量。结果雌激素组SD大鼠子宫内膜NO含量较对照组显著升高(P<0.01),孕激素组NO含量较对照组显著降低(P<0.01)。结论雌激素、孕激素可调节子宫内膜NO含量。     

分光光度法测定复方刺五加片中总黄酮含量研究

目的建立分光光度法测定复方刺五加片中总黄酮含量的方法,为复方刺五加片的质量控制提供参考。方法采用分光光度法,以芦丁为对照品、采用510 nm波长处对复方刺五加片中总黄酮含量进行测定。结果芦丁对照品在(8.32~58.24)mg/L的浓度范围内呈良好的线性关系(r=0.999 7),平均加样回收率为99.96%,RSD=4.19%(n=6),3批复方刺五加片样品中总黄酮含量分别为10.05、9.98、10.01 mg/片,平均为10.01 mg/片。结论分光光度法测定复方刺五加片中总黄酮含量方法操作简单易行,测定结果可靠,可作为复方刺五加片总黄酮的检测及质量控制。     

放射免疫法测定兔血浆18甲基炔诺酮

用自制的18甲基炔诺酮-3-羧甲基肟-牛血清白蛋白(Ng-3-CMO-BSA)免疫新西兰兔,首次免疫后8个月抗体滴度为1:5000,抗体与18甲基炔诺酮(Ng)的亲和常数(K_A)为7:84×10~9(mol/L)~(-1)。灵敏度为12.5Pg,回收率>90％,批内差异<9％,批间差异<17%。抗体除了与炔诺酮有20％的交叉反应外,与其他8种甾体激素的交叉反应均<0.1％。     

二阶导数分光光度法和紫外分光光度法测定炔诺孕酮炔雌醚片中炔雌醚和炔诺孕酮的含量

采用二阶导数分光光度法对炔诺孕酮炔雌醚片中炔雌醚的含量进行了测定；采用紫外分光光度法测定炔诺孕酮的含量。该方法不需预先分离，简单、快速，其精密度及准确度均较好。同时，采用一般分光光度计进行手动式二阶导数分光光度法对比实验，也获得了满意的结果。     

二阶导数分光光度法和紫外分光光度法测定炔诺孕酮炔雌醚片中炔雌醚和炔诺孕酮的含量

采有二阶导数分光光度法对炔诺孕酮炔雌醚中炔雌醚的含量进行了测定；采用紫外分光光度法测定炔诺孕酮的含量，该方法不需预无分离，简单，快速，其精密及准确度均较好，同时，采用一般分光光度计进行手动式二阶导数分光光度法对比实验，也获得了满意的结果。     

dl-18-甲基炔诺酮-[2,3,d]异(口恶)唑及其卤代物的合成

dl-18-甲基炔诺酮是现用的避孕药之一。甾体化合物引入[2,3 d]异噁唑环或C_(21)卤代后生物活性均有不同程度的提高。我们参考文献方法,合成了dl-18-甲基炔诺酮-[2,3 d]-异噁唑2;并采用简便的方法,合成了化合物2的卤代物3、4及炔诺酮的C_(21)     

dl-18-甲基炔诺酮-[2,3,d]异(口恶)唑及其卤代物的合成

dl-18-甲基炔诺酮是现用的避孕药之一。甾体化合物引入[2,3 d]异噁唑环或C_(21)卤代后生物活性均有不同程度的提高。我们参考文献方法,合成了dl-18-甲基炔诺酮-[2,3 d]-异噁唑2;并采用简便的方法,合成了化合物2的卤代物3、4及炔诺酮的C_(21)     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…