心舒康胶囊质量标准研究

目的:建立心舒康胶囊质量控制标准.方法:采用薄层色谱(TLC)法对处方中葛根、川芎、丹参、赤芍进行了定性鉴别研究;采用反相高效液相色谱法对样品中的葛根素进行了含量测定.结果:TLC定性鉴别斑点清晰,灵敏度高,色谱特征明显,专属性强;含量测定葛根素的平均回收率为99.72%,RSD为1.49%(n=6).结论:该法简便、准确、专属性强,可作为心舒康胶囊的质量控制方法.     

寒热痹胶囊质量标准研究

目的:建立寒热痹胶囊的质量标准.方法:采用薄层色谱法,对寒热痹胶囊中知母、干姜、甘草进行薄层定性鉴别.采用高效液相色谱法对其中的芍药苷进行含量测定,采用Dikma Diamonsil C18(4.6 mm×250 mm,5μm)色谱柱;流动相:乙腈-0.1%:磷酸溶液(14:86);流速:1.0 ml/min:检测波长:230 nm.结果:在薄层色谱中可检出知母、干姜、甘草的特征斑点.芍药苷在0.112 4～1.124 0μg范围内与峰面积呈良好的线性关系(r=0.999 9),平均回收率为101.20%,RSD=1.71%(n=6).结论:本方法简便、准确、重现性好,可用于寒热痹胶囊的质量控制.     

肝宁胶囊的质量标准研究

目的:对肝宁胶囊质量标准进行研究。方法:采用薄层色谱法对处方中的紫草进行定性鉴别;采用气相色谱法对处方中的斑蝥素进行含量测定。结果:在薄层色谱中可检出紫草的特征斑点;斑蝥素在0.013 8μg⁰.124 2μg(r=0.999 9)范围内线性关系良好,回收率为98.466%(RSD=1.92%,n=5)。结论:本方法简便可行、重复性好,能有效控制该制剂的质量。     

RP-HPLC和TLCS测定黄连解毒汤有效部位中藏红花酸含量的方法研究

目的　建立黄连解毒汤有效部位中藏红花酸的含量测定方法。方法　采用高效液相色谱法(HPLC)及薄层色谱扫描法 (TLCS)。HPLC法 :YWG C18色谱柱 ;流动相 :甲醇—水—冰乙酸 ;检测波长 :4 2 3nm。薄层色谱扫描法 :硅胶G薄层板 ;展开剂 :氯仿—甲醇—甲酸 ;反射法锯齿扫描 ,入射波长 :4 10nm ;参比波长 :5 6 0nm。结果　HPLC法平均回收率为 10 1 6 8% ,RSD =1 16 % (n =5 ) ;TLCS法平均回收率为 98 5 4% ,RSD =0 83% (n =5 )。结论　两种方法测定结果基本一致 ,且操作简便、准确 ,均可作为黄连解毒汤有效部位的质量控制方法。     

萸藿胶囊质量标准研究

目的:建立萸藿胶囊的定性、定量检测方法。方法:采用薄层色谱法对萸藿胶囊中山茱萸、淫羊藿、女贞子、桑椹进行定性鉴别;采用高效液相色谱法测定萸藿胶囊中马钱苷的含量。结果:山茱萸、淫羊藿、女贞子、桑椹的薄层色谱斑点清晰、分离效果较好、且无阴性干扰。马钱苷的质量浓度在5.16~41.24μg/m L范围内与其峰面积积分值呈良好线性关系(r=0.9999);精密度、稳定性、重复性试验的RSD均≤2.0%;平均加样回收率为98.89%,RSD=3.08%(n=6)。结论:该方法简便可行、专属性强、重复性好,可用于萸藿胶囊的质量控制。     

前列宝胶囊中黄芪甲苷的含量测定

目的 :研究前列宝胶囊质量控制方法。方法 :采取聚酰胺柱层析法分离提取前列宝胶囊中黄芪甲苷 ,用薄层扫描法测定黄芪甲苷含量。结果 :黄芪甲苷的回归方程为 :s=76 97.6 6 +85 2 8.97×c ,r=0 .9984。线性范围 1 .8～ 9.0 μg ,平均回收率为 1 0 1 .4 1± 5 .2 3% ,RSD为 2 .89%。结论 :薄层扫描法测定前列宝胶囊中黄芪甲苷含量可作为该制剂的质量控制方法     

心舒康胶囊质量标准研究

目的:建立心舒康胶囊质量控制标准.方法:采用薄层色谱(TLC)法对处方中葛根、川芎、丹参、赤芍进行了定性鉴别研究;采用反相高效液相色谱法对样品中的葛根素进行了含量测定.结果:TLC定性鉴别斑点清晰,灵敏度高,色谱特征明显,专属性强;含量测定葛根素的平均回收率为99.72%,RSD为1.49%(n=6).结论:该法简便、...     

金莲清热胶囊质量标准研究?

目的提高和完善金莲清热胶囊的质量标准。方法采用薄层色谱法(TLC)对金莲清热胶囊中金莲花、大青叶、玄参和苦杏仁进行定性鉴别;采用高效液相法(HPLC),色谱柱为Phenomenex Luna C18(2)100A(250 mm×4.6 mm,5μm),以乙腈-0.4%乙酸铵溶液(15∶85)为流动相,流速1.0 m L/min,检测波长340 nm,柱温30℃,进行牡荆苷含量测定。结果金莲花、大青叶、玄参和苦杏仁的薄层色谱主斑点清晰,分离良好;牡荆苷在0.06~0.57μg(r=0.999 9)线性良好,平均加样回收率为99.7%,RSD为2.1%。结论建立的TLC和HPLC方法专属性强、准确、重复性好,可用于金莲清热胶囊的质量控制。     

脑康灵A胶囊质量标准研究

目的:建立脑康灵A胶囊的质量控制方法。方法:采用薄层色谱法(TLC)对脑康灵A胶囊中的川芎、丹参、车前子进行定性分析,采用高效液相色谱法(HPLC)测定制剂中川芎的阿魏酸含量。以十八烷基硅烷键合硅胶为填充剂;色谱柱:C18柱(250 mm×4.6 mm,5μm);流动相:乙腈-0.085%磷酸溶液(13∶87);检测波长:321 nm;流速:1.0 m L/min,进样量10μL。结果:川芎、丹参、车前子的薄层色谱鉴别供试品色谱中,在与对照药材色谱相应的位置上,显相同颜色的斑点,分离较好,阴性对照无干扰。阿魏酸质量浓度在100.8~604.8 ng范围内与其峰面积积分值成良好线性关系(r=0.9999);精密度、稳定性、重复性实验的RSD3%;平均加样回收率为101.04%,RSD=2.93%(n=6)。结论:该方法操作简单、灵敏度高、专属性强、重复性好,可作为脑康灵A胶囊的质量控制方法。     

RP-HPLC法测定兔血浆中羟基喜树碱的浓度

目的 :建立测定羟基喜树碱在兔血浆中浓度的反相高效液相色谱 -紫外检测法。方法 :血浆样品酸化后 ,用甲醇沉淀蛋白 ,高速离心后取上清液进行色谱分析。色谱柱 :LichrospherC1 8柱 ( 2 5 0mm× 4.6mm ,5 μm ) ,C1 8预柱 ( 10mm× 4.6mm ,5 μm) ;流动相 :ψ(甲醇 ∶ 0 .0 1mol/L磷酸盐缓冲液 ,pH4.0 ) =60∶40 ;流速 :1.0mL/min ;检测波长 3 84nm。以 5mg/kg剂量给大耳白兔静脉推注羟基喜树碱注射液 ,于不同时间点采血测定药物浓度。结果 :羟基喜树碱保留时间为 5 .0min ,定量线性范围为 5 0～ 5 0 0 0ng/mL ,最低检测限为 2 5ng/mL。血浆中羟基喜树碱的回收率为 99.88%～ 10 3 .13 %,日内精密度≤ 3 .2 7%,日间精密度≤ 7.2 5 %。羟基喜树碱静脉注射后符合二室模型。结论 :本法简便实用 ,定量准确 ,可满足羟基喜树碱药动学研究的需要     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.