美满霉素等9种药物对人全血吞噬细胞和非细胞体系的抗活性氧作用

观察和比较美满霉素，四环素，灭滴灵，青霉胺，氯喹，布洛芬，头孢位定，异搏定和三氟拉嗪对中性粒细胞（ＰＭＮ）产生的ＲＯＳ和非细胞体系产生的Ｏ＾２和ＯＨ的影响，应用化学发光法（ＣＬ）测定人全血吞噬细胞在受调理的酵母多糖诱导下产生的活性氧（ＲＯＳ），黄嘌呤－黄嘌呤化酶体系所的Ｏ２和ＶｉｔＣ－ＣｕＳＯ４－酵母多糖－Ｈ２Ｏ２体系产生的ＯＨ。结果，所有９种药物都具有抑制或清除中性粒细胞产生的ＲＯＳ，美满霉素，     

APNF0134清除活性氧及抗超氧阴离子诱导的血小板聚集作用的…

以化学发光法测定了（１）黄嘌呤－－黄嘌呤氧化酶及碱性二甲亚砚二体系产生的Ｏ２；（２）Ｈ２Ｏ２的释放；（３）ＶｉｔＣ－Ｈ２Ｏ２－Ｃｕ＾２＋－酵母多糖产生ＯＨ。观察ＡＰＮＦ０１３４这体系产生的活性氧的影响。并以比浊法观察了Ｏ２诱导的人活活血小板的聚集及ＡＰＮＦ０１３４对其的影响。结果表明ＡＰＮＦ０１３４具有清除Ｏ２（两经产生）、Ｈ２Ｏ２、ＯＨ及抑制Ｏ２诱导的血小板聚集作用，呈霁旧依赖性，ＩＣ５０依次为     

维拉帕米对人血PMN细胞呼吸暴发和氧自由基的作用

用化学发光法观测了维拉帕米对人血ＰＭＮ细胞受ＰＭＡ刺激发生呼吸暴发产生的活性氧，黄嘌呤－黄嘌呤氧化酶体系产生的Ｏ，ＶｉｔＣ－Ｃｕ￣（２＋）－酵母多糖体系产生的·ＯＨ，及Ｈ＿２Ｏ＿２的释放反应的影响。结果表明，维拉帕米对ＰＭＮ细胞的呼吸暴发全过程均有显著的抑制作用：对Ｏ有清除作用；对Ｈ＿２Ｏ＿２的释放反应有抑制作用，并呈剂量依赖性，ＩＣ＿（５０）分别为１３６ｎｍｏｌ／Ｌ，２８．１ｎｍｏｌ／Ｌ和２２．３ｎｍｏｌ／Ｌ；对·ＯＨ则无作用。     

维拉帕米对人血PMN细胞呼吸暴发和氧自由基的作用

用化学发光法观察了维拉帕米对人血ＰＭＮ细胞受ＰＭＡ刺激发生呼吸暴发生的活性氧，黄嘌呤－黄嘌呤－黄呤氧化酶体系产生的Ｏ＾－２，ＶｉｔＣ－Ｃｕ＾２＋－母多糖体系产生的．ＯＨ，及Ｈ２Ｏ２的释放反应的影响。     

红细胞作载体的谷胱甘肽过氧化物酶和超氧化物歧化酶对脑缺血再灌注的影响

目的研究采用红细胞（ＲＢＣ）作为超氧化物歧化酶（ＳＯＤ）和谷胱甘肽过氧化物酶（ＧＳＨ·Ｐｘ）的载体的可行性，并将这些载体ＲＢＣ用于兔的脑缺血－再灌注研究。方法采用低渗透析法将ＳＯＤ和ＧＳＨ·Ｐｘ装进ＲＢＣ，测量装入量和载体ＲＢＣ在兔体内的循环半寿期。通过测量外周血浆脂质过氧化物（ＬＰＯ）和一氧化氮（ＮＯ），并做脑组织的ＴＴＣ染色来观察其作用效果。结果被装入的ＳＯＤ和ＧＳＨ·Ｐｘ的ＲＢＣ，在兔体内的循环半寿期分别为１３．７±１．０天和７．３±２．５天，兔自身ＲＢＣ的值为１３．４±１．５天。对照组血浆ＮＯ浓度在再灌注１０分钟时达最高值。ＲＢＣ－ＳＯＤ组在再灌注４．５小时期间，ＮＯ持续较高浓度状态。ＲＢＣ－ＧＳＨ·Ｐｘ组和联合给药组在再灌注期间，ＮＯ浓度的升高受到抑制。在再灌注期间，对照组和ＲＢＣ－ＧＳＨ·Ｐｘ组的ＬＰＯ升高，ＲＢＣ－ＳＯＤ组和联合给药组的ＬＰＯ无明显升高。ＴＴＣ染色结果表明，ＲＢＣ－ＳＯＤ、ＲＢＣ－ＧＳＨ·Ｐｘ和ＲＢＣ－ＳＯＤ＋ＲＢＣ－ＧＳＨ·Ｐｘ可有效减轻脑缺血—再灌注损伤。结论ＲＢＣ可用作ＳＯＤ和ＧＳＨ·Ｐｘ的载体，它们对活性氧引起的脑缺血再灌注损伤有较好的防治作用。     

药物气相色谱分析衍生化方法推荐专家系统的设计与实现

介绍了药物气相色谱分析衍生化方法推荐专家系统。该系统可对分子量小于５００,且分子中含有－ＣＯＯＨ、－ＣＯＯ－、－ＯＨ、－Ｏ＾－、－ＮＨ２（包括Ｒ－２、＝ＣＯＮＨ２、－ＳＯ２ＮＨ２）、ＮＨ（包括Ｒ１ＮＨＲ２、－ＣＯＮＨＲ、－ＣＯＮＨＳＯ２－、－ＳＯ２ＮＨＲ、Ｒ１ＮＨＣＯＮＨＲ２）、Ｒ４Ｎ（＋＋、Ｃ＝、＝ＣＨＯ、－ＳＨ、＝ＳＯ３Ｈ、－ＳＯ３＾－等 有团的药物实现衍生化方法推荐,并能自动调用柱系统推荐子     

神经多肽P物质对中性粒细胞质膜功能蛋白的影响

目的探索神经多肽对中性粒细胞质膜功能蛋白的影响。方法Ｐ物质（ｓｕｂｓｔａｎｃｅ　Ｐ,ＳＰ）与中性粒细胞（Ｎｐ）共温育后,用ＮＢＴ和ＨＶＡ方法测定呼吸爆发水平（以Ｏ２·－　、Ｈ２Ｏ２　相对量表示）,生化法测定质膜酸性磷酸酶（ａｃｉｄｉｃ　ｐｈｏｓｐｈａｔａｓｅ,ＡＣＰ）、碱性磷酸酶（ａｌｋａｌｉｎｅ　ｐｈｏｓｐｈａｔａｓｅ,ＡＬＰ）活性,荧光标记法测定补体受体３型（ＣＲ３）、白细胞表面共同抗原ＣＤ４５及趋化三肽ＦＭＬＰ受体表达的变化。结果Ｓｐ促进Ｎｐ　Ｏ２·－、Ｈ２Ｏ２水平上升,可以使Ｎｐ质膜ＡＣＰ活性降低,而ＡＬＰ无变化,使ＣＤ４５、ＣＲ３和ＦＭＬＰ３种受体的表达量增加。结论神经多肽ＳＰ在促进呼吸爆发的同时,对Ｎｐ质膜功能蛋白的影响具有广泛性和多样性,表明ＳＰ可能影响Ｎｐ参与炎症反应的各个进程。     

山药水溶性多糖的化学及体外抗氧化活性

山药经热水提取、去蛋白、乙醇沉淀，ＳｅｐｈａｄｅｘＧ－１００纯化得山药多糖（ＲＰ），分子量为８．１×１０４。气相色谱分析ＲＰ主要含葡萄糖及少量岩藻糖。经过碘酸氧化及Ｓｍｉｔｈ降解后气相色谱分析结果显示各组分的摩尔比为岩藻糖－丙三醇－赤藓醇是１：１．２４：６．４２，证明ＲＰ主要由带有分枝的１→４连接的吡喃糖苷骨架构成，同时含有少量１→３键型的岩藻糖。该多糖能降低维生素ＣＮＡＤＰＨ及Ｆｅ（２＋）－半胱氨酸诱发的微粒体过氧化脂质的含量，并对黄嘌呤－黄嘌呤氧化酶体系产生的超氧自由基（Ｏ（２－））及Ｆｅｎｔｏｎ反应体系产生的羟自由基（ＯＨ－）有清除作用。     

辅酶B12Costa型模型化合物的合成与表征

合成了以Ｎ，Ｎ一双（２，３－丁二酮－２－亚胺－３－肟）丙二胺为平面配体的烷基钴Ｓｃｈｉｆｆ碱－肟类辅酶Ｂ１２Ｃｏｓｔａ型模型化合物［Ｒ Ｃｏ（ＤＯ）（ＤＯＨ）ｐｎ·Ｈ２Ｏ］，其中，Ｒ＝ｎＣ３Ｈ７；ｎ－Ｃ４Ｈ７；ｉＧ４Ｈ９；ｎＣ５Ｈ１１；Ｃ－Ｃ６Ｈ１１；Ｃ６Ｈ５ＣＨ２，用紫外可见光谱、红外光谱、元素分析和ＨＮＭＲ进行了表征，讨论了不同Ｒ基加入对配合物的影响，为探讨辅酶Ｂ１２诱导的重排反应机理，提供了     

氟西汀与氯丙咪嗪治疗强迫性神经症的临床对照研究

目的 搪塞氟西汀对强迫性神经症（强迫症）的临床疗效及副作用。方法 ４５例强迫症患者随机分成氟西汀组（２４例）、氯丙咪嗪组（２１例）进行平行对照研究。采用Ｙａｌｅ－Ｂｒｏｗｎ强迫量表（Ｙ－ＢＯＣＳ）、Ｈａｍｉｌｏｎ抑有限 量表（ＨＡＭＤ）和副反应量表分别评定疗效和副作用。结果 两组患者治疗Ｙ－ＢＯＣＳ和ＨＡＭＤ评分均比治疗前降低、两组患者Ｙ＝ＢＯＣＳ和ＨＡＭＤ的减分率差异均针显著性意义。氟西汀组显著     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.