不同强度脉冲电磁场对大鼠骨质疏松的影响

目的:观察不同强度脉冲电磁场(PEMFs)对去势后大鼠骨质疏松症的影响.方法:30只雌性SD大鼠行双侧卵巢切除术建立骨质疏松动物模型,随机分为3组(n=10):磁场组暴露于强度分别为4×10-4 T,15×10-4 T的PEMFs环境中(f=15 Hz,τ=5 ms),暴磁时间为6 h/d;对照组饲养于线圈中,但不暴磁.12 wk后,对血清骨钙素、骨密度以及生物力学等参数进行检测.结果:与对照组相比,实验组骨密度、最大载荷及结构刚度均有增加(P＜0.05),血清骨钙素水平减少(P＜0.05);两个磁场组之间作比较,各指标的差异也具有统计学意义(P＜0.05).结论:PEMFs有益于骨质疏松症的预防和治疗,磁场参数的选择对其疗效有影响.     

低强度脉冲电磁场对大鼠骨密度及骨形态计量学的影响

目的:探讨低强度脉冲电磁场对大鼠骨密度及生物力学特性的影响. 方法:将32只3 mo龄雌性SD大鼠随机分为4组(n=8):去势组、对照组、磁场A组、磁场B组;各组大鼠均采用下腹部切口,去势组及磁场组行双侧卵巢切除术,对照组仅行开腹术. 其中,磁场A组暴露于强度为4×10-4 T的脉冲磁场环境中,磁场B组暴露于强度为8×10-4 T的脉冲磁场环境中,两组磁场频率f均为15 Hz,τ为5 ms,暴磁时间为6 h/d. 各组动物饲养条件相同,8 wk后处死,测定全身骨密度及骨形态计量学指标. 结果:与去势组大鼠相比,磁场组全身骨密度、股骨远端骨密度、骨小梁面积百分比、骨小梁宽度、骨小梁数目均有增加(P<0.05),骨小梁间隙减小(P<0.05);而与对照组相比,磁场B组全身骨密度、骨小梁面积百分比以及骨小梁数目等指标间的差异则没有显著性意义(P>0.05);两磁场组之间作比较,全身骨密度和骨小梁数目的差异显著(P<0.05). 结论:PEMFs对卵巢切除大鼠骨质疏松症的发生有一定的预防和治疗作用,磁场生物效应存在明显的窗口效应.     

低强度脉冲电磁场暴露对兔血液流变特性及血脂水平的影响

目的　研究低强度脉冲电磁场预防心血管疾病的机制 .方法　 2 4只雌性新西兰大耳白兔随机分为正常组 ( )、模型组 ( )和磁场组 ( ) . 组用正常饲料 , 和 组用高胆固醇饲料饲养 .其中 组饲养于磁场环境中 ,暴磁时间为10 h· d- 1 ,所采用激励磁场的强度为 4× 10 - 4T、频率为 15Hz.饲养 8wk后测定其血液流变特性和血脂水平等指标 .结果　 组与 组比较 ,血细胞比容、血浆粘度、全血高切粘度、全血低切粘度、高切还原粘度、低切还原粘度、红细胞聚集指数以及胆固醇、三酰甘油、高密度脂蛋白等指标均有显著性差异 (P<0 .0 5 ) ; 组与 组比较 ,血脂中胆固醇、三酰甘油以及血液流变中的血细胞比容、血浆粘度、全血低切粘度指标降低显著 (P<0 .0 1) ,而高密度脂蛋白升高显著 (P<0 .0 1) .结论　低强度脉冲电磁场能够显著降低血脂、血液流变学等指标 ,提高血液流动性 ,对心血管疾病有良好的预防作用     

低强度脉冲电磁场对兔心血管系统的影响

目的: 探讨低强度脉冲电磁场(pulsed electromagnetic fields,PEMFs)对心血管系统的影响,为心血管疾病的预防和治疗提供新的思路和理论依据.方法: 30只雌性5 mo龄新西兰大耳白兔随机等分为3组: ①对照组用常规饲料喂养.②高脂组用高脂、高胆固醇饲料饲养.③磁场组用高脂、高胆固醇饲料饲养,并暴露于强度为8×10-4 T的脉冲磁场环境中(f=15 Hz).饲养8 wk后,分别检测血液流变学、血脂以及心功能等指标,并对主动脉进行解剖学观察,对心肌与肝脏进行组织病理学检查.结果: ①磁场组的血液流变学指标与高脂组比较,差异有显著性意义(P<0.01);与对照组比较,磁场组只有全血高切黏度(ηb1)、低切还原黏度(ηb2′)和红细胞聚集指数(AI)等3项指标的差异具有显著性意义(P<0.01).②与高脂组比较,磁场组的血清总胆固醇和三酰甘油降低明显(P<0.01),高密度脂蛋白反而有明显升高(P<0.01);与对照组比较,磁场组三酰甘油和高密度脂蛋白的差异没有显著性意义(P>0.05);③与高脂组比较,磁场组各项心功能指标的差异均具有显著性意义(P<0.01);与对照组比较,磁场组LVET,PEP,ICT以及ICT/LVET均没有显著性差异(P>0.05).④对主动脉内壁大体观察,肉眼清晰可见高脂组弥漫有大量条纹状白色斑块,致使管壁增厚、变硬、管腔狭窄;磁场组内膜表面仅见少量针头大小斑点,血管弹性正常;对照组内膜光滑,未见任何病变.⑤肝脏HE染色后,镜下观察高脂组可见大量脂肪空泡,而磁场组与对照组肝组织无明显病变.⑥心肌HE染色后,镜下可见高脂组细胞质中出现大小不等的脂肪空泡,散在于肌原纤维间,而磁场组与对照组镜下观察无明显病变.结论: 低强度PEMFs对心血管疾病有明显的预防和治疗作用.     

脉冲电磁场对高胆固醇饮食兔左室功能的影响

目的:探讨低强度脉冲电磁场对长期进食高胆固醇饲料兔心功能的影响. 方法: 将30只雌性5 mo龄新西兰大耳白兔随机分为3组(n=10): 对照组、高脂组、磁场组. 对照组用常规饲料喂养,高脂组和磁场组用高胆固醇饲料饲养. 磁场组暴露于强度为4 Gs的脉冲电磁场环境中(f=15 Hz),暴磁时间为10 h/d;饲养8 wk后测定左室功能指标. 结果: 与对照组比较,高脂组左室射血时间显著缩短,射血前期和等容收缩期明显延长,PEP/LVET和ICT/LVET均显著增加(P《0.01),而磁场组的差异没有统计学意义(P》0.01);与高脂组比较,磁场组左心室射血时间延长(P《0.01)、射血前期及等容收缩期缩短(P《0.01)、PEP/LVET及ICT/LVET比值显著降低(P《0.01). 结论: 低强度脉冲电磁场能够显著改善左室功能.     

稳恒磁场对高血脂小鼠血液流变学的影响

目的：研究稳恒磁场对高血脂小鼠血液流变学的影响。方法：16只小鼠用胆固醇饲料喂养6周，建立高血脂模型成功后随机分为对照组和磁场组，每组8只，正常饲料喂养6周，其中磁场组小鼠放置于磁场环境中，暴磁时间为8 h/ d，磁场强度为0.36 T；6周时测定其高切全血黏度、低切全血黏度等血液流变学指标。结果：磁场组血液流变学指标明显低于对照组（ P ﹤0.05）。结论：稳恒磁场可以提高血液的流动性，改善血液循环，可能有利于高血脂的治疗。     

极低频电磁场对小鼠血细胞影响的研究

目的通过观察不同照度的极低频电磁场对小鼠血液细胞成分影响及淋巴细胞微核发生的关系,推测极低频电磁场对人类血液系统的影响,以及导致人类疾病发生的机制,为人类合理利用电磁波资源、预防疾病的发生提供理论依据.方法将100只健康的小鼠随机分为对照组和曝磁组.曝磁组小鼠置于微机所产生的极低频电磁场环境中,每天将曝磁组小鼠分别放入极低频电磁场中照射,曝磁1组照射20min,曝磁2组照射40min,曝磁3组照射60min,曝磁4组照射6h;对照组小鼠不接触极低频电磁场,共4个月,分别取各组小鼠血液进行外周血血细胞计数和淋巴细胞微核检测.结果随着小鼠每天曝磁时间的增加,其血液中的红细胞、白细胞、血小板数量及血红蛋白含量呈现逐渐下降的趋势,淋巴细胞微核率逐渐出现并呈现增高的趋势,从曝磁2组的表现开始明显,曝磁4组表现最为显著.但仅有红细胞数量、血红蛋白含量和淋巴细胞微核率与对照组结果之间存在显著性差异(P＜0.05或P＜0.01).白细胞分类呈现中性粒细胞、嗜酸性粒细胞、单核细胞的比例逐渐增高、淋巴细胞的比例逐渐下降的趋势,其中以曝磁4组小鼠血液中白细胞比例改变最为显著.结论长时间接触极低频电磁场对小鼠血液细胞数量和不同白细胞比值存在明显的影响,同时可诱发淋巴细胞微核的发生.揭示极低频电磁场对人体血液系统可能存在一定的影响,建议人们应合理利用电磁波资源,预防电磁场给人类带来的危害.     

极低频脉冲磁场生物效应的实验研究

目的：研究极低频（ＥＬＦ）脉冲磁场的生物效应,方法：选择不同频率,不同功率的ＥＬＦ脉冲磁场,对小鼠、人离体血、细菌等实验对象进行不同时间长度的照射,照射后地小鼠的血液流变学指标,自由基 血细胞的变化,对小鼠的学习记忆能力,人离体血血液流变学指标和细力的生化指标及质粒ＤＮＡ进行测量,研究脉冲磁场作用对这些指标的影响。结果：脉冲电磁场可使血液的表观粘度降低;可抑制小鼠骨髓生成白细胞的功能;可影响大肠杆     

低强度脉冲电磁场对小鼠血液流变学指标的影响

探讨低强度脉冲电磁场对小鼠血液流变学指标的影响。方法将小鼠分为４组,前３组分别暴露于脉冲频率为１５Ｈｚ,脉冲时延为５ｍｓ,强度分别为２＊１０＾－４Ｔ,４＊１０＾－４Ｔ,８＊１０＾－４Ｔ的磁感应强度下,时间为１０ｄ,每日６ｈ,后１组为对照组,第１０日统一右眼取血,测定其血液流变学指标中的血细胞比容及全血高切、低切表观粘度。结果低强度低频脉冲电磁场辐照组与对照组比较,除２＊１０＾－４Ｔ组的红细胞聚集     

脉冲电磁场对大鼠骨髓间充质干细胞体外诱导分化影响的研究

目的观察脉冲电磁场(PEMFs)对5-氮胞苷(5-aza)诱导大鼠骨髓间充质干细胞(MSCs)向心肌样细胞分化的影响。方法50Hz脉冲低频电磁场刺激5-aza诱导7d后的MSCs,根据不同的感应强度和作用的时间分为A、B、C组和1、2、3、4亚组,设未暴露PEMFs的为对照组。通过Western印迹法检测不同条件下肌钙蛋白T(cTnT)的表达量的变化。结果在0.5、1、5mT组中20、30min各亚组较对照组有显著性增加,在5mT组在10min的cTnT的表达量显著性减少。结论50Hz的0.5~5mT感应强度的PEMFs作用20~30min为促进体外5-aza诱导MSCs向心肌方向分化的有效窗口。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.