怀牛膝与红牛膝、紫花地榆与白花地榆的紫外谱线组法鉴别

应用紫外吸收光谱谱线组法鉴别（怀）牛膝ＡｃｈｙｒａｎｔｈｅｓｂｉｄｅｎｔａｔａＢＬ．及红牛膝ＡｃｈｙｒａｎｔｈｅｓｂｉｄｅｎｔａｔａＢｌｕｍｅｆ．ＲｕｂｒａＨＣＯ．的根，紫花地榆ＳａｎｇｕｉｓｏｒｂａｏｆｉｃｉｎａｌｉｓＬ．及白花地榆ＳａｎｇｕｉｓｏｒｂａｃａｎａｄｅｎｓｉｓＬ．根的研究。     

怀牛膝与红牛膝,紫花地榆与白花地榆的紫外谱线组法鉴别

应用紫外吸收光谱谱线组法鉴别牛膝Ａｃｈｙｒａｎｔｈｅｓ ｂｉｄｅｎｔａｔａ ＢＬ．及红牛膝Ａｃｈｙｒａｎｔｈｅｓ ｂｉｄｅｎｔａｔａ Ｂｌｕｍｅ ｆ．Ｒｕｂｒａ ＨＣＯ．的根，紫花地榆Ｓａｎｇｕｉｓｏｒｂａ ｏｆｆｉｃｉｎａｌｉｓ Ｌ．及白花地榆Ｓａｎｇｕｉｓｏｒｂａ ｃａｎａｄｅｎｓｉｓ Ｌ．根的研究。     

黄芪研究进展

黄芪研究进展安徽中医学院附属医院刘道芳袁敏（２３００３１）黄芪为豆科的蒙古黄芪（ＡｓｔｒａｇａｌｕｓｍｅｍｂｒａｎｃｅｕｓＢｇｅ．Ｖａｒ．ｍｏｎｇｈｏｌｉｃｕｓ（Ｂｇｅ．）Ｈｓｉａｏ）或膜荚黄芪（Ａ．ｍｅｍｂｒａｎｃｅｕｓ（Ｆｉｓｃｈ）Ｂｇｅ．）的...     

中药瞿麦的鉴别研究

本文对中国药典［１］收载的中药瞿麦品种瞿麦（ＤｉａｎｔｈｕｓｓｕｐｅｒｂｕｓＬ．）和石竹（Ｄ．ｃｈｉｎｅｎｓｉｓＬ．）的根与茎的组织特征及叶片的显微常数进行了研究，为其品种鉴别提供依据     

云南红景天化学成分的研究

云南红景天化学成分的研究云南省药品检验所（６５００１１）何广新日本冈山大学药学部吉田隆志云南红景天［Ｒｈｏｄｉｏｌａｙｕｎｎａｎｅｎｓｉｓ（Ｆｒａｎｃｈ）Ｓ．Ｈ．Ｆｕ］是景天科（Ｃｒａｓｓｕｌａｃｅａｅ）红景天属植物。具有接筋骨，除风湿，解毒消肿之功...     

北野菊挥发油化学成分的研究

北野菊挥发油化学成分的研究中国医学科学院药用植物资源开发研究所（北京１０００９４）关玲，权丽辉，沈一行，陈建民北野菊［Ｃｈｒｙｓａｎｔｈｅｍｕｍｌａｖａｎｄｕｌａｅｆｏｌｉｕｍ（Ｆｉｓｃｈ．）Ｍａｋｉｎｏ］又名甘野菊或岩香菊，它是其同属植物野菊［Ｄｅ...     

DDPH对实验性高血压大鼠血管平滑肌细胞增殖及对PDGF-B,bFGF,c-sis,c-myc的影响

目的：观察１（２，６二甲基苯氧基）２（３，４二甲氧基苯乙胺基）丙烷盐酸盐（ＤＤＰＨ）对实验性高血压大鼠血管平滑肌细胞（ＶＳＭＣ）增殖的作用及对生长因子ＰＤＧＦＢ，ｂＦＧＦ及其相关癌基因ｃｓｉｓ，ｃｍｙｃ表达的影响。方法：采用氚胸腺嘧啶核苷（３ＨＴｄＲ）掺入法，电镜，免疫组化，原位杂交法进行实验观察。结果：ＤＤＰＨ在降低血压同时，能减少ＶＳＭＣ的线粒体，粗面内质网和３ＨＴｄＲ掺入量，并能逆转ＶＳＭＣ增殖时ＰＤＧＦＢ，ｂＦＧＦ抗原及ｃｓｉｓ，ｃｍｙｃｍＲＮＡ的表达增强。结论：ＤＤＰＨ能抑制实验性高血压大鼠的ＶＳＭＣ增殖，与生长因子及癌基因调控的分子生物学机制有关。     

苦参碱对体外培养小鼠心肌细胞感染柯萨奇B3病毒的影响

近年来，病毒性心肌炎发病率显著上升，尤其在青壮年中，而临床上尚缺乏有效的防治药物。许多报道已证实了该病的致病原因主要与肠道病毒，特别是ＣＶＢ３病毒有关［１］。但目前国内外对本病尚无公认的有效治疗措施而致使临床治疗处于被动的状态。本实验采用细胞化学染色，心肌酶及ＭＤＡ含量测定等方法观察苦参碱（Ｓｏｐｈｏｒａ Ｆｌａｖｅｓｃｅｎｓ Ａｉｔ ＳＦＡ）对ＣＶＢ３感染的心肌细胞的保护作用，旨在为临床治疗病毒性心肌炎（ＶＭＣ）的药物选择提供实验依据。１材料和方法１．１材料出生１～２ｄ的ＢＡＬＢ／Ｃ乳鼠由我院实…     

五倍子与文蛤

五倍子与文蛤广饶县人民医院（２５７３００）张洪顺，隋红关键词五倍子，文蛤，中药研究五倍子为漆树科植物盐肤木ＲｈｕｓｃｈｉｎｅｎｓｉｓＭｉｌｌ青麸杨ＲｈｕｓｐｏｔａｎｉｍｉＭａｘｉｍ或红麸杨ＲｈｕｓｐｕｊａｂｅｎｓｉｓｓｔｅｗＶａｒＳｉｎｉｃａ（Ｄｉｃ...     

地黄药理研究进展

地黄药理研究进展白求恩国际和平医院（０５００８２）李兰青地黄为玄参科植物地黄［１］Ｒｅｈｍａｎｎｉａｇｌｕｔｉｎｏｓａｌｉｂｏｓｃｈ·［Ｒ·ｇｌｕｔｉｎｏｓａＬｉｂｏｓｃｈ·ｆ·ｈｕｅｉｃｈｉｎｇｅｎｓｉｓ（ｃｈａｏｅｔｓｃｈｉｈ）ＨＳｉａｏ］的干燥...     

拉雅松和细叶云南松松针挥发油的化学成分

目的:研究拉雅松和细叶云南松松针挥发油的化学成分.方法:采用水蒸汽蒸馏法从拉雅松和细叶云南松松针中提取挥发油,利用气相色谱-质谱联用技术分析挥发油的化学成分.结果:从拉雅松松针挥发油中鉴定出62个化合物,占挥发油总量的95.565％,主要成分为α-蒎烯(24.659％)、β-蒎烯(11.471％)、β-石竹烯(9.932％)和异松油烯(5.167％);从细叶云南松松针挥发油中鉴定出55个化合物,占挥发油总量的90.794％,主要成分为α-蒎烯(22.542％)、β-石竹烯(16.636％)和1,2,4a,5,8,8a-六氢化-4,7-二甲基-1-(1-甲基乙基)-萘(5.085％).结论:首次研究了拉雅松和细叶云南松松针挥发油的化学成分,为合理开发利用广西的松针资源提供了科学依据.     

Effects of Polygala tenuifolia root extract on proliferation of neural stem cells in the hippocampal CA1 region

Neurogenesis persists in the adult mammalian brain and can be a target for modulation for therapeutic purposes. This study investigated the effect of a Polygala tenuifolia root extract on the proliferation of a stem cell population in the rat hippocampus. The root extract of P. tenuifolia (2 mg/kg/day, 14 times intraperitoneal injections) increased the incorporation of bromodeoxyuridine (BrdU) into cells in the hippocampal CA1 region. This activity was enriched in the saponin-containing fraction. The majority of cells labelled with BrdU were immunoreactive to nestin or Tuj1 and the percentages of nestin/BrdU- and Tuj1/BrdU-double positive cells were increased by the P. tenuifolia root extract, suggesting that the P. tenuifolia root extract promotes the proliferation of neural stem cells. In addition, this extract promoted the neurite outgrowth of rat neuronal precursor cells, HiB5. These activities of P. tenuifolia root extract may contribute to the therapeutic benefits of herbal medicines containing P. tenuifolia root for the treatment of patients with insomnia, neurosis and dementia.     

河北太行山区远志最佳播种期的确定

目的为太行山区远志播种期的确定提供科学依据。方法本试验设置六个播种期,并比较苗期情况以及根性状和远志酸含量。结果播种时期对远志的生长影响较大。结论远志的最佳播种期为夏末秋初的雨季。     

Stability examination of the inclusion compounds of 8 Chinese medicinal volatile oils with beta-cyclodextrin

The content, hence the stability of volatile oils and their main components in the inclusion compounds of 8 Chinese medicinal volatile oils with beta-cyclodextrin have been examined with different storage times. The 8 Chinese medicines are Vitex negundo var. heterophylla, Notopterygium incisum, Alpinia officinarum, Atractylodes lancea, Pogostenon cablin, Asarum heterotropoides var. mandshuricum, Schizonepeta tenuifolia and Amomum villosum.     

Effect of Polygala tenuifolia root extract on cerebral ischemia and reperfusion

In this study, the effects of Polygala tenuifolia root extract on brain ischemia/reperfusion injury in Mongolian gerbils were investigated. The gerbils were administered ethanol extract of P. tenuifolia and its four sub-fractions orally 2 hours prior to ischemia, and were subjected to a 20-minute no-flow cerebral ischemia in vivo. Thirty minutes and 72 hours after reperfusion, the brain was removed and the ATP, lactate and lipid peroxide levels were determined, and the neurons in the hippocampal CA1 subfield were examined. In the vehicle-treated ischemic gerbils, the brain ATP levels decreased significantly, but this decrease was prevented by pre-treatment with an n-butanol fraction of P. tenuifolia. In contrast, both the lactate content and lipid peroxidation levels were elevated in the vehicle-treated ischemic animals, but this elevation was inhibited by ethanol extract and n-butanol fraction of P. tenuifolia, respectively. Both the ethanol extract and n-butanol fraction of P. tenuifolia attenuated post-ischemic neuronal necrosis in the hippocampal CA1 subfield. Our findings suggest that both ethanol extract and n-butanol fraction of P. tenuifolia root can reduce brain damage during ischemia and reperfusion, and prevent lipid peroxidation and preserve the energy metabolism.     

沉香超微粉与山沉香超微粉药效学对比分析

目的:研究沉香超微粉、原粉抗心肌缺血、耐缺氧、镇痛与镇静的药理学作用,并与山沉香超微粉作对比研究。方法:冠脉结扎方法制造大鼠心肌缺血模型,并观察沉香与山沉香各给药组大鼠心梗面积,心电图,血清肌酸激酶(CK),超氧化物歧化酶(SOD),乳酸脱氢酶(LDH);钠石灰法观察沉香与山沉香各给药组小鼠存活时间;乙酸扭体法和戊巴比妥钠协同法观察沉香与山沉香各给药组对小鼠的镇痛、镇静作用。结果:沉香超微粉、原粉和山沉香超微粉均有不同程度抗大鼠心肌缺血作用:可降低大鼠血清CK,LDH的活性,提高血清SOD的活性,并能降低心肌缺血的面积,明显改善心电图(P0.05,P0.01),且沉香超微粉0.75,0.375 g·kg~(-1)剂量组明显优于沉香原粉(P0.05);相同剂量沉香超微粉与山沉香超微粉作用相当;沉香、山沉香各给药组对小鼠缺氧状态下存活时间均有延长作用(P0.05),相同剂量下山沉香超微粉作用优于沉香超微粉(P0.05);沉香和山沉香超微粉组可明显延长小鼠扭体潜伏期(P0.05),并减少扭体次数(P0.05),同剂量下山沉香超微粉与沉香超微粉组作用相当;山沉香和沉香超微粉对阈剂量戊巴比妥钠致小鼠睡眠时间均有明显延长作用(P0.05)。山沉香超微粉与对应剂量沉香超微粉组作用相当。结论:沉香超微粉、原粉与山沉香超微粉均有抗心肌缺血、耐缺氧、镇痛、镇静等作用,且同剂量下沉香超微粉作用优于其原粉,山沉香超微粉作用相当于对应剂量的沉香超微粉。     

常用藏药"松蒂"(篦齿虎耳草)的显微鉴别研究

目的:建立常用藏药材“松蒂”(篦齿虎耳草)的显微鉴定方法.方法:对“松蒂”主流品种篦齿虎耳草及其近似种爪瓣虎耳草、唐古特虎耳草和青藏虎耳草的性状、显微特征进行比较组织学研究.结果:明确了篦齿虎耳草药材性状、各部位(根、茎、叶、花、花梗及果实)组织构造、粉末显微特征;建立了4种虎耳草的药材性状、茎横切面、叶横切面及粉末鉴别方法.结论:根据基生叶着生形态、毛茸类型、花序类型、小花数目、茎各组织部位比例、花粉粒形态等的特征能有效地鉴别“松蒂”的主流品种篦齿虎耳草及其近似种.     

荆芥选育品系农艺性状及品质性状比较

目的:在相同条件下进行荆芥品系比较试验,为选育高产优质荆芥药材提供科学依据.方法:田问实验采用随机区组设计,收获期调查其主要农艺性状;采用水蒸气蒸馏法测定挥发油含量,HPLC测定胡薄荷酮含量.结果:S4品系比CK1增产18.63%,比CK2增产29.99%,达到极显著水平,且挥发油和胡薄荷酮含量也高于其他参比品系和对照;S16品系与CK产量上无显著差异,抗病性比较强.结论:S4品系具有增产潜力大、有效成分高等优点,有望选育成在生产上推广的优良品种.     

远志的化学成分研究(Ⅰ)

目的研究中药远志Polygalatenuifolia根中的化学成分.方法采用硅胶、Sephadex LH-20及ODS柱层析进行分离纯化,通过理化性质和光谱分析鉴定结构.结果从中分离得到了5个化合物,分别鉴定为tenuifolisideB(Ⅰ),3,4,5-三甲氧基肉桂酸甲酯(Ⅱ),polygalaxanthone Ⅲ(Ⅲ),7-O-methylmangiferin(Ⅳ)和lancerin(Ⅴ).结论化合物Ⅱ和Ⅳ为首次从远志属植物中分离得到,化合物Ⅴ为首次从远志植物中分离得到.     

Stem Blight Control of Schizonepeta tenuifolia Caused by Phytophthora nicotianae Using Trichoderma spp.

Objective To control stem blight disease of Schizonepeta tenuifolia caused by Phytophthora nicotianae. Methods The antagonist effect of 13 Trichoderma strains (including T. viride and T. harzianum) was evaluated upon mycelia growth of P. nicotianae. Trichoderma strains with high antagonistic activities against the pathogen were used to control stem blight of S. tenuifolia in the field. Results Of 13 Trichoderma strains tested, T. viride strain M3 showed maximum mycelia growth inhibition (83.2%) to the patho...