白介素-16基因多态性与广西地区鼻咽癌遗传易感性的研究

目的研究广西地区人群白介素-16(IL-16)基因多态性与鼻咽癌(NPC)遗传易感的相关性。方法对来自广西地区的75例鼻咽癌患者和75例健康体检者采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)及酶联免疫吸附法,分析IL-16基因的3个单核苷酸多态性(SNP)位点(rs11556218T/G、rs4778889T/C、rs4072111C/T)多态性与NPC的相关性。结果 IL-16基因rs11556218位点在NPC患者中TG基因型分布频率(49.3%)显著高于健康对照组(34.7%)(P=0.037)。NPC组IL-16-rs11556218G等位基因频率为32.7%,高于正常对照组的21.3%(P=0.027),并且G等位基因携带者的血清IL-16水平明显高于TT基因型(P=0.00)。携带GTT和GCC单倍型的个体罹患鼻咽癌风险性是携带TTC单倍型者的2.95倍和2.73倍。结论 IL-16基因rs11556218位点的基因多态性与广西地区鼻咽癌的遗传易感性有关,TG基因型可能是NPC发生的易感基因型,携带G等位基因的个体患NPC风险增高。     

汉族人群慢性牙周炎患者IL-13和IL-4基因多态性及连锁不平衡研究

目的 研究汉族人群慢性牙周炎患者白介素13(IL-13)和IL-4基因多态性分布情况及其与慢性牙周炎发病的可能关系.方法 采用基质辅助激光解吸离子化-飞行时间质谱法(MALDI-TOF-MS)对慢性牙周炎组(n=278)和健康对照组(n=324)IL-13基因中rs2070874和rs1800925及IL-4基因中rs2243248的单核苷酸多态性(SNP)进行检测,计算等位基因频率和基因型分布,并进行连锁不平衡及单倍型分析.结果 慢性牙周炎组与健康对照组rs1800925的TT基因型和T等位基因与rs2243248的GG基因型和G等位基因频率的差异有统计学意义(P＜0.05);两组间rs2070874 T/rs1800925 T/rs2243248 G单倍型及另外两种单倍型rs2070874 T/rsl800925 C/rs2243248 G与rs2070874 T/rs1800925 T/rs2243248 T的分布差异也有统计学意义(P＜0.05).结论 IL-13基因rs 1800925和IL-4基因rs2243248的SNP可能与中国汉族人群慢性牙周炎的发病易感性相关.     

广西红水河流域长寿群体Smyca基因多态性分布及其对代谢危险因素的影响

目的：探究广西红水河流域长寿人群Smyca基因多态性的分布情况及其对衰老相关代谢指标的影响。方法：使用PCRSanger测序法对广西红水河流域长寿老人95例（长寿组，年龄≥90岁）及当地普通健康老人94例（对照组，年龄60～75岁）的Smyca基因进行多态性检测，使用Haploview分析单倍型的连锁不平衡（LD），并采用Pearson相关性分析方法来分析单倍体携带情况及长寿风险评估。结果：在Smyca基因中共检测出9个多态，其中长寿组rs2594713 AA基因型及等位基因A的频率显著低于对照组，CC基因型及等位基因C的频率则高于对照组（均P<0.05），其他基因型与等位基因频率在两组间的分布无明显差异（P>0.05）。3个单核苷酸多态性（SNPs）;rs2594713、rs7255672和rs62122064位点之间存在较高的LD；长寿组ATA单倍型频率低于对照组（P<0.05）；在长寿人群中，rs2594713、rs7255672和rs62122064位点的野生纯合型携带者甘油三酯（TG）及载脂蛋白B(ApoB)水平均低于突变型携带者（均P<0.05），其...     

Spl10基因与肺结核易感相关性研究

目的:研究Spl 10基因rs1135791、rs722555、rs116799831、rs3948464位点基因多态性与重庆市汉族人群肺结核发病的关系.方法:采用病例对照研究设计,检测rs1135791、rs722555、rs116799831、rs3948464位点SNP多态性,用SAS软件进行显著性检验.结果:(1)Spl 10基因rsll35791 CT基因型、m722555 GG基因型、rs116799831 GG基因型、rs3948464 CC基因型在病例组的频率明显高于对照组;(2)病例组rs1135791 C等位基因、rs722555 G等位基因、rs116799831 G等位基因、rs3948464C等位基因频率都高于对照组,差异有统计学意义.结论:rs1135791 C/T、rs722555 A/G、rs116799831 A/G、rs3948464 C/T可能是重庆市汉族人群肺结核易患的危险因素.     

白三烯C4合成酶基因多态性与缺血性脑卒中关系

目的探讨白三烯C4(LTC4)合成酶基因启动子区rs730012(-444A/C)位点多态性与缺血性脑卒中(IS)的相关性。方法应用聚合酶链反应限制性片段长度多态性(PCR-RFLP)及凝胶电泳技术,检测IS组和与其性别及年龄相匹配的健康对照组LTC4合成酶基因启动子区rs730012位点多态性,并比较两组基因型、等位基因频率的差异。采用Logistic回归去除传统危险因素的影响,分析其基因型、等位基因频率与IS发病相关性。结果IS组rs730012位点非携带C等位基因(AA基因型)人群患IS的风险约是携带C等位基因(AC+CC基因型)人群的1.6倍(OR=1.636,P<0.05)。结论 LTC4合成酶基因rs730012位点多态性可能与IS发病相关,等位基因A可能是其风险等位基因。     

Apelin基因与原发性高血压的相关性

目的 探讨apelin基因多态性及单倍型与原发性高血压的相关关系.方法 选取上海地区汉族人群原发性高血压家系248家共1 042人为研究样本,应用TaqMan MGB荧光探针定量PCR技术,检测各样本apelin基因启动子区T-1860C和rs3761581基因多态性分型.应用FBAT软件预测该两个多态性位点可能组成的单倍型,并对多态位点及单倍型与原发性高血压的关系进行分析.结果 所选位点基因型频率在研究人群中的分布均符合Hardy-Weinberg遗传平衡定律.应用FBAT统计分析结果显示T-1860C、rs3761581及其组成的单倍型A-T与原发性高血压相关.其中T-1860C的T等位基因(Z=1.991,P=0.046)和rs3761581的A等位基因(Z=2.119,P=0.034)可以由亲代下传给患病子代.结论 位于apelin基因启动子区的多态性位点与上海地区汉族人群原发性高血压存在相关关系,单倍型A-T可能参与高血压的发病.     

甘肃汉族人群溶质载体家族30成员8基因多态性与2型糖尿病的相关性

目的探讨甘肃汉族人群溶质载体家族30成员8(SLC30A8)基因多态性与2型糖尿病的相关性。方法采用聚合酶链式反应-限制性片段长度多态性分析法,随机选取甘肃116例2型糖尿病患者(2型糖尿病组)及80例体检者(对照组)进行SLC30A8基因rs13266634C/T单核苷酸多态性检测,比较两组间基因型频率和等位基因频率及相关性;以稳态模型胰岛B细胞分泌功能指数评估胰岛B细胞功能,胰岛素抵抗指数评估胰岛素抵抗。结果 2型糖尿病组CC基因型频率明显高于对照组,而TT基因型频率低于对照组。2型糖尿病组CC基因型频率与TT基因型频率比较,有显著性差异(P<0.01);C风险等位基因患2型糖尿病的风险是T等位基因者的2.40倍,有显著性差异(P<0.01);CC基因型的胰岛B细胞分泌功能指数、空腹胰岛素显著低于TT基因型,差异有统计学意义P<0.05)。CC、CT、TT3种基因型的胰岛素抵抗指数,差异无统计学意义。结论甘肃汉族人群存在SLC30A8基因rs13266634多态性,rs13266634多态性与甘肃汉族2型糖尿病的发生相关;SLC30A8基因rs13266634的C等位基因可能是2型糖尿病的风险等位基因。SLC30A8基因增加2型糖尿病的易感性可能与胰岛B细胞功能下降有关,与胰岛素抵抗无明显相关性。     

P-选择素基因单核苷酸多态性及单倍型与冠心病的相关性研究

目的:研究P-选择素(P-selectin)基因单核苷酸多态性及其单倍型与冠心病易感性之间的关系.方法:以210例冠心病患者和220例健康对照者为研究对象,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法对P-selectin基因-1969 G/A和-1817 T/C单核苷酸多态性进行基因分型.结果:P-selectin基因-1969 G/A多态性在冠心病组和正常人群中的分布差异无统计学意义(P＞0.05),而P-selectin基因-1817 T/C多态性在两组人群中的分布差异有统计学意义(P＜0.05),等位基因频率的相对风险分析发现,-1817 C等位基因携带者患冠心病的风险是T等位基因的1.547倍(OR=1.547,95%CI:1.170-2.045,P=0.002);联合基因型分析发现,P-selectin的-1969 A/-1817 C基因单倍型携带者频率在冠心病组中显著高于对照组(P＜0.05).与对照组比较,-1969 A/-1817 C基因单倍型携带者显著增加了冠心病的发病风险(OR=2.134,95%CI:1.273～3.576,P=0.003).结论:P-selectin基因-1817 T/C多态性和-1969 A/-1817 C单倍型与冠心病的发病具有相关性,其中-1817 C等位基因可能是冠心病的遗传易感基因.     

NTNG1基因多态性与精神分裂症的关系

目的 探讨中国北方汉族人群中NTNG1基因rs4132604、rs2218404、rs1373336基因多态性与精神分裂症的关系.方法 采用聚合酶链式反应-限制性内切酶片段长度多态性(PCR-RFLP)的方法,检测中国北方汉族316例精神分裂症患者及311例健康对照者的基因型,应用遗传统计学方法进行等住基因型及等位基因频率分析、单倍型分析.结果 病例组和对照组NTNG1基因的rs4132604、rs2218404、rs1373336基因型频数分布均符合Hardy-Weinberg平衡定律(P>0.05);rs4132604等位基因频率(χ2=7.86,P=0.01)和基因型分布(χ2=7.67,P=0.02)在病例组和对照组间存在显著性差异,提示该基因在中国北方汉族人群中同精神分裂症的发病明显相关.病例组与对照组比较,rs2218404、rs1373336等位基因频率和基因型分布未见显著性差异.在单倍型分析中,rs4132604与rs2218404中的GG(χ2=6.11,P=0.01)和TG(χ2=5.28,P=0.02)、rs2218404与rs1373336中的GT(χ2=5.38,P=0.02)和GC(χ2=4.72,P=0.03)、三者中GGT(χ2=13.40,P=0.00)和TGT(χ2=6.56,P=0.01),两组间存在显著性差异.结论 NTNG1基因的rs4132604多态位点同精神分裂症存在关联.IWNG1基因的rs4132604、rs2218404、rs1373336构成的单倍型上存在同精神分裂症发病的相关位点.     

中国汉族人群中YWHAE基因与帕金森病的相关性研究

目的 探讨YWHAE基因多态性与中国汉族人群帕金森病(PD)之间的相关性.方法 采用TaqMan检测法对中国汉族人群258例PD患者(病例组)和260例健康体检者(对照组)YWHAE的3个位点(rs1873827、rs12452627、rs1532976)进行关联分析和单核苷酸多态性分析,比较病例组和对照组等位基因频率、基因型频率及单倍型的差异.结果 两组间 YWHAE的3个位点基因频率、基因型频率比较差异无统计学意义.rs1873827与rs12452627 的LD分析其D'值较大(D'=0.933).进一步对两位点的单倍型分析发现其各种组合间差异均无统计学意义.结论 研究结果提示YWHAE基因的3个位点与中国汉族人群PD的发生不存在相关性.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.