恒河猴静脉内皮细胞衬里ePTFE人工血管的实验研究

按原位获取法恒河猴表浅静脉内皮细胞，在体外培养扩增，制备成内皮细胞悬液。用纤维蛋白胶和纤维连接蛋白联合预衬各ｅＰＴＦＥ人工血管腔，将内皮细胞灌入此人工血管，结果内皮细胞的贴壁率明显提高；内皮细胞高密度种植人工血管后２ｈ，扫描电下见人工血管腔表面已形成完整的内皮细胞单层；孵育９ｄ后，透射电镜见内皮细胞骨架已分化成熟，表明此时人工血管腔内皮细胞具有良好的抗切应能力。     

恒河猴静脉内皮细胞衬里ePTFE人工血管的实验研究

按原位获取法获取恒河猴表浅静脉内皮细胞，在体外培养扩增。制备成内皮细胞悬液（2×109／L）。用纤维蛋白胶和纤维连接蛋白联合预衬各ePTFE人工血管腔（长10cm，直径3mm），将内皮细胞灌入此人工血管。结果内皮细胞的贴壁率明显提高［（94．62±2．84）％］；内皮细胞高密度种植入工血管后2h，扫描电镜下见人工血管腔表面已形成完整的内皮细胞单层；孵育9d后，透射电镜见内皮细胞骨架已分化成熟，表明此时人工血管腔内皮细胞具有良好的抗切应能力。     

阿胶预衬人工血管对内皮细胞粘附作用的影响

用中药阿胶预衬人工血管后种植内皮细胞（ＥＣ）。结果显示：阿胶能明显提高ＥＣ在人工血管的粘附率，其中以１０％浓度阿胶效果最好，而且对ＥＣ的生长和形态均有良好影响。阿胶的作用与纤维结合素相近，优于明胶和白蛋白。主要成份蛋白质多为非糖链蛋白，可能抑制血小板在人工血管的粘附而减少血栓形成的机会     

细胞外基质对内皮细胞粘附功能的影响及意义

目的：促进内皮细胞在聚氨酯材料上粘附，为实现人工心血管内皮细胞化探索条件和方法。方法：用荧光法观察细胞外基质成分对内皮细胞在聚氨酯材料上粘附的影响。结果：用胶原Ｉ，胶原Ⅳ，纤维粘连蛋白（ＦＮ）玻璃粘连蛋白（ＶＮ）等细胞外基质成分及对照组牛血清白蛋白（ＢＳＡ）分别预衬聚氨酯５０ｍｇ／Ｌ浓度组，内皮细胞与材料接触１５ｍｉｎ后，粘附率分别为３２．３１％，５４．４３％，４２．３４％，６３．７７％和２７．７     

纤维连接蛋白在肾脏疾病中变化的意义

测２０例尿毒症患者血浆纤维连接蛋白含量，证实尿毒症者血浆Ｆｎ降低，并与患者中性粒细胞吞噬功能低下有关联。体外实验证实Ｆｎ有促进ＰＭＮ吞噬功能作用，且与其浓度正相关。表明血浆Ｆｎ与机体免疫防御功能关系密切，其水平可反映患者细胞吞噬功能状态。     

单向免疫扩散与火箭电泳测定血浆纤维结合蛋白的比较及临床应用

采用单向免疫扩散法与火箭电泳法测定血浆纤维结合蛋白（ＦＮ）比较，结果一致（ｒ＝０．９９９６），单扩法测４０例正常人群血浆ＦＮ合量为２９０．４８±４０．１１μｇ／ｍｌ。另外测８０例不同类型肝病患者ＦＮ含量，结果血浆ＦＮ水平高低不能反映肝脏纤维化的指标。     

小口径内皮化人工血管的临床应用研究

目的探讨小口径内皮化人工血管的临床应用效果。方法将成人大隐静脉内皮细胞在体外培养（１２．５４±１．２８） ｄ;扩增培养的自体细胞衬里　于纤维蛋白胶和纤维连接蛋白联合预衬的国产 ｅＰＴＦＥ人工血管,继续培养 ９ ｄ。将此人工血管行股－　动脉旁路搭桥术。结果细胞数增加（１７８．２６±４４．９３）倍。细胞种植后 ２ ｈ和 ９ ｄ,人工血管腔面见一层均匀的基质,基质表面有一层连续的内皮细胞单层。内皮细胞排列紧密,呈梭形状,形态饱满。除１例术后２月出现人工血管血栓形成外,其余１０例患者经６～３６月的随访检查,人工血管通畅良好。结论双期法离休衬里技术可有效地提高小口径人工血管的通畅率。     

β－受体激活对血小板激活因子、组胺致血管壁通透性增高的抑制作用

本实验采用整体动物和体外灌注内皮细胞单层模型，研究了异丙肾上腺素（ＩＰＮ）对炎症介质引起的血管壁通透性增加的影响。结果表明，大鼠静注伊文斯蓝２０ｍｇ／ｋｇ后，皮内注射１０￣（－７）ｍｏｌ／ＬＰＡＦ或０．１ｍｇ／ｍｌ组胺０．２ｍｌ，分别引起局部伊文斯蓝渗出１６．８２±２．０５μｇ／ｇ组织和２１．６８±２．８６μｇ／ｇ组织。预先静注ＩＰＮ１０μｇ／ｋｇ，伊文斯蓝渗出量分别减少５６．２％和４３．６％。心得安（ＰＰＮ）可逆转ＩＰＮ的作用。单纯心得安处理，使ＰＡＦ和组胺引起的伊文斯蓝渗出量增高，提示生理浓度的儿茶酚胺对血管壁通透性有抑制作用。体外灌注内皮细胞单层研究表明，ＩＰＮ可显著降低ＰＡＦ引起的跨内皮单层液体流量和蛋白清除率的增高，从细胞水平证实ＩＰＮ可抑制ＰＡＦ引起的血管壁通透性增高。     

层粘连蛋白对两株小鼠肝癌克隆细胞系体外附壁及浸润的影响

Ｈｃａ－Ｆ和Ｈｃａ－Ｐ细胞系是从小鼠癌中分离出来的具有不同转移能力的两株克隆。在Ｃ３Ｈ／ｈｅｊ小鼠实验组显示，Ｆ细胞比Ｐ细胞具有较强的侵袭力和淋巴道转移能力。在体外附壁实验中，Ｆ细胞比Ｐ细胞更易贴附于层粘连蛋白上，而且，在后者加上Ⅳ型胶原基质后，Ｆ细胞的贴壁能力更增强；纤维连接蛋白加上Ⅳ型胶原可促进Ｐ细胞贴壁。     

不同麻醉方法对血浆纤维连接蛋白的影响

不同麻醉方法对血浆纤维连接蛋白的影响孔娟，张秉钧宁跃，李鹏飞（第二临床学院麻醉科）（同位素研究中心）关键词血浆纤维粘接蛋白；全身麻醉；硬膜外麻醉纤维连接蛋白（Ｆｉｂｒｏｎｅｃｔｉｎ，ＦＮ）是一种存在于血浆和细胞表面的高分子糖蛋白，具有多种生理功能，它...     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.