冠心病患者血栓前状态的研究

目的 :观察冠心病患者血浆纤溶酶原激活物的抑制物 - 1(PAI- 1)、血栓素B2 (TXB2 )、6 -酮 -前列腺素F1α(6 -K -PGF1α)等反映血栓前状态分子标志物含量变化 ,并探讨它们与冠心病的关系。方法 :选择符合 1979年WHO诊断标准或经冠脉造影证实的冠心病患者 6 0例为冠心病组 ,其中 ,稳定型心绞痛 (SAP) 17例 ,不稳定型心绞痛 (UAP) 31例 ,急性心肌梗死 (AMI) 12例。同时 ,选择年龄、性别、等配对的正常志愿者 2 0例作为对照组。分别用酶联吸附双抗体夹心法、放射免疫法检测血浆PAI - 1和ET、TXB2 、6 -K -PGF1α含量。结果 :①冠心病患者血浆PAI- 1、ET、TXB2 含量显著高于对照组 (P <0 .0 1)、6 -K -PGF1α含量显著低于对照组 (P <0 .0 1)。②SAP、UAP、AMI三组患者血浆中PAI- 1、ET含量依次升高 ,差异均有显著性 (P <0 .0 5 ) ;TXB2 、6 -K -PGF1α含量在AMI、SAP、UAP三组之间比较 ,TXB2 在AMI组升高更显著 (P <0 .0 1) ,6 -K -PGF1α在AMI组下降更显著 (P <0 .0 1) ,而在SAP、UAP两组之间比较差异均无显著性 (P >0 .0 5 )。结论 :冠心病患者存在血栓前状态 ,表现为纤溶功能障碍、血管内皮细胞受损和血小板激活等。     

血栓前状态与高血压病的关系

目的判断原发性高血压是否存在血栓前状态,并探讨血栓前状态在原发性高血压中的意义。方法运用酶联免疫吸附双抗体夹心法(ELLSA)、酶联免疫吸附竞争法、发色底物法、凝固法等手段,检测了1 000例原发性高血压病患者和100例健康成人的6-酮-前列腺素Fla(6-K-PGFla)含量、血栓素B2(TXB2)、纤维蛋白原(Fbg)、组织型纤溶酶原激活剂(t-PA)及纤溶酶原激活抑制物-1(PAI-1)的含量和活性、血液流变学等指标的变化并进行分析与评价。结果1-2级高血压组及3级高血压组病人,血浆TXB2、PAI-1含量及活性、血黏度、Fbg水平均明显高于对照组(P< 0.01);而t-PA含量及活性、6-K-PGFl a含量均显著下降(P<0.01)。结论高血压病患者存在血栓前状态。血栓前状态与高血压患者的病情发展,血栓栓塞性疾病的发生密切相关。     

榛蘑多糖对三氯化铁所诱导大鼠血栓的抑制作用

[目的]探讨榛蘑多糖对三氯化铁所诱导大鼠血栓的抑制作用.[方法]将SD大鼠随机分为假手术组、模型组和用药低、高剂量组(100,400mg/kg),给予三氯化铁诱导建立大鼠颈动脉血栓模型.用药组灌胃给予不同剂量榛蘑多糖,每日1次,连续灌胃7d后检测血栓质量,ELISA法测定血浆组织纤溶酶原激活物(t-PA)、纤溶酶原激活物抑制剂(PAI-1)、6-酮-前列腺素E1α(6-keto-PGE1α)和血栓素B2(TXB2)含量,用硝酸还原酶法检测一氧化氮水平.[结果]与模型组比较,用药低、高剂量组血栓质量显著降低,t-PA、一氧化氮和6-keto-PGE1α水平均显著升高,TXB2含量显著降低(P<0.05,P<0.01).[结论]榛蘑多糖具有抗血栓作用.     

高血压病患者血栓前状态的研究（摘要）

目的 :探讨高血压病易并发血栓性疾病的机制。方法 :分别采用放射免疫法、发色底物显色法、ＥＬＩＳＡ法等检测 16例健康人 (对照组 )、5 3例高血压病 1、2、3级患者血浆中的分子标志物———血栓烷Ｂ2 (ＴＸＢ2 )、6 -酮 -前列腺素Ｆ1α(6 -Ｋ-ＰＧＦ1α)、组织型纤溶酶原激活物 (ｔ-ＰＡ)、组织型纤溶酶原激活物抑制物 (ＰＡⅠ - 1)及血液流变学指标。结果 :1- 2级高血压组及 3级高血压组病人 ,血浆ＴＸＢ2 、ｔ-ＰＡ含量、ＰＡⅠ- 1含量及活性、血粘度、纤维蛋白原水平均明显高于对照组 (Ｐ <0 0 5 ) ;而ｔ -ＰＡ活性、6 -Ｋ -ＰＧＦ1α…     

香椿子抗血栓作用的实验研究

目的:观察香椿子的抗血栓作用。方法:对大鼠凝血酶原时间(PT)、大鼠活化部分凝血活酶时间(APTT)、大鼠凝血酶时间(TT)进行测定来观察香椿子对这些指标的影响。复制大鼠静脉血栓模型并称取血栓湿重来观察香椿子的抗血栓作用。对血瘀证大鼠模型血浆TXB2和6-Keto-PGF1α含量及血浆纤溶酶原激活物(t-PA)、纤溶酶原激活抑制物(PAI)含量的影响。结果:①对大鼠PT、APTT及TT的影响:香椿子三个剂量组均显著延长了大鼠PT及TT。980 mg/kg剂量组与生理盐水组比较能显著延长大鼠的APTT;②对大鼠血栓湿重的影响:香椿子700、980 mg/kg剂量组的血栓湿重与空白对照组比较均有显著性差异;③对血瘀证大鼠模型血浆TXB2和6-Keto-PGF1α含量的影响:香椿子水煎液对血浆TXB2含量并无明显影响。而香椿子中、高剂量组能显著升高血浆6-Keto-PGF1α含量;④对血瘀证大鼠模型血浆纤溶酶原激活物(t-PA)、纤溶酶原激活抑制物(PAI)含量的影响:香椿子水煎液各组与生理盐水组均无统计学差异。结论:香椿子有较强的抗血栓作用。     

深静脉血栓形成患者急性期和恢复期纤溶功能的变化

目的探讨下肢深静脉血栓(DVT)形成患者急性期和恢复期纤溶功能的变化。方法选择急性期DVT患者38例,恢复期患者63列,对照组45例,实验室中分别测定纤溶酶原(Plg)、组织纤溶酶原激活物活性(t-PA:A)、组织纤溶酶原激活物抑制剂-1(PAI-1)、组织纤溶酶原激活物含量(t-PA:Ag)水平。结果急性期t-PA:A和恢复期的Plg、t-PA:A、t-PA:Ag、PAI-1均比正常降低(P<0.05,P<0.01):而恢复期Plg、t-PA:A和PAI-1比急性期降低更明显(P<0.05,P<0.01)。结论无论急性期还是恢复期,DVT患者纤溶功能均较正常减弱。     

t-PA,PAI-1检测在脑梗死患者中的应用

目的:探讨组织型纤溶酶原激活物(t-PA)和纤溶酶原激活抑制物(PAI-1)含量及t-PA/PAI-1比值在脑梗死患者中的临床意义。方法:用酶联免疫发色底物法分别测定53例脑梗死患者和30例健康对照组血浆t-PA、PAI-1含量。结果:脑梗死患者血浆中t-PA、PAI-1及t-PA/PAI-1比值与正常对照组比较均有显著性差异(P<0.01)。结论:检测患者血t-PA、PAI-1水平及t-PA/PAI-1比值对脑梗死患者的诊断和治疗有重要意义。     

TNF-α、t-PA/PAI-1在2型糖尿病血管并发症中的意义

目的探讨血清肿瘤坏死因子(TNF-α)、血浆组织型纤溶酶原激活物(t-PA)与血浆纤溶酶原激活物抑制物(PAI-1)的比值在2型糖尿病(DM)有血管并发症患者中的变化。方法检测40例DM有血管并发症及40例DM无血管并发症患者TNFα-、t-PA/PAI-1水平并进行比较。结果DM血管并发症患者TNF-α水平明显增高(P<0.01);t-PA/PAI-1比值明显减低(P<0.01)。结论TNF-α、t-PA/PAI-1检测对DM血管并发症发生及患病情况分析有重要的临床价值。     

2型糖尿病血管并发症TNF-α、t-PA/PAI-1变化

通过检测血清肿瘤坏死因子α（TNF-α）、血浆组织型纤溶酶原激活物（t-PA）与血浆纤溶酶原激活物抑制物（PAI-1）的比值，证明2型糖尿病（DM）血管并发症患者TNF-α水平明显增高，t-PA／PAI-1比值明显减低。以便正确分析2型糖尿病（DM）血管并发症发生及预后情况。     

血府逐瘀汤对动脉血栓模型大鼠纤溶活性的影响

目的 观察血府逐瘀汤对动脉血栓模型大鼠血浆中组织型纤溶酶原激活物（t-PA）、纤溶酶原激活物抑制剂-1（PAI-1）、纤溶酶原（PLG）含量和t-PA/PAI-1 比值的影响.方法 SD 大鼠60 只,雌雄各半,随机分为空白组、模型组、阳性对照组、血府逐淤汤高、中、低剂量组,连续灌胃给药14 d,空白组和模型组予以生理盐水,用三氯化铁外敷颈总动脉复制血栓模型,观察血栓质量和测定血浆中t-PA、PAI-1、PLG 含量.结果 模型组颈总动脉有明显的血栓形成,说明造模成功;与模型组比较,药物组血栓质量均显著降低,其中血府逐瘀汤中剂量组血栓质量最轻,差异有统计学意义（P〈0.01）;与模型组比较,血府逐瘀汤能明显升高大鼠血浆中t-PA 含量和t-PA/PAI-1 比值、显著降低PAI-1、PLG 含量,差异有统计学意义（P〈0.01）.结论 血府逐瘀汤通过升高t-PA 含量、降低PAI-1、PLG 含量,调节t-PA/PAI-1 比值,达到纠正t-PA、PAI-1 之间的平衡失调的作用.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.