两种不同仪器用于嗜酸性粒细胞测定的精密度比较

目的 探讨比较不同仪器用于测定嗜酸性粒细胞的精密度,确定仪器的临床使用价值.方法 利用Sysmex XE-2100全自动血细胞分析仪和显微镜目测法对30例正常人(正常组)和157例临床患者(疾病组)标本进行嗜酸性粒细胞的测定,对数据进行统计处理;选择3份嗜酸性粒细胞数值(高、中、低)抗凝静脉血分别采用仪器法和目测法进行计数,每份标本均重复计数10次,计算出CV值.结果 仪器法和显微镜目测法对正常组和疾病组嗜酸性粒细胞数的测定结果无显著性差异(P＞0.05),但仪器法和显微镜目测法分别对高、中、低值3份标本的重复性实验的嗜酸性粒细胞值分别为4.18%、7.46%和11.3%,批内平均为7.65%.而目测法为9.99%、10.8%和24.4%,批内平均嗜酸性粒细胞为15.1%.二者比较具有显著性差异(P＜0.01),仪器法由于目测法.结论 XE-2100全自动血细胞分析仪的精密度优于显微镜目测法,简便、快捷,可满足临床诊断、治疗和疗效观察的需要.     

两种不同仪器用于嗜酸性粒细胞测定的精密度比较

目的探讨比较不同仪器用于测定嗜酸性粒细胞的精密度,确定仪器的临床使用价值。方法利用Sysmex XE-2100全自动血细胞分析仪和显微镜目测法对30例正常人(正常组)和157例临床患者(疾病组)标本进行嗜酸性粒细胞的测定,对数据进行统计处理;选择3份嗜酸性粒细胞数值(高、中、低)抗凝静脉血分别采用仪器法和目测法进行计数,每份标本均重复计数10次,计算出CV值。结果仪器法和显微镜目测法对正常组和疾病组嗜酸性粒细胞数的测定结果无显著性差异(P>0.05),但仪器法和显微镜目测法分别对高、中、低值3份标本的重复性实验的嗜酸性粒细胞值分别为4.18%、7.46%和11.3%,批内平均为7.65%。而目测法为9.99%、10.8%和24.4%,批内平均嗜酸性粒细胞为15.1%。二者比较具有显著性差异(P<0.01),仪器法由于目测法。结论XE-2100全自动血细胞分析仪的精密度优于显微镜目测法,简便、快捷,可满足临床诊断、治疗和疗效观察的需要。     

全自动血细胞分析仪嗜酸性粒细胞计数性能的评价

目的 了解迈瑞BC-5200全自动血细胞分析仪嗜酸性粒细胞计数结果的可靠性.方法 随机抽取100份经EDTA-K2抗凝静脉全血,在BC-5200全血动血细胞分析仪上检测后制作成血涂片,经显微镜进行分类计数.同时用目测法进行嗜酸性粒细胞直接计数,将仪器分类嗜酸性粒细胞结果与手工分类法.仪器嗜酸性粒细胞绝对值计数结果与目测法结果分别进行比较.结果 100份抗凝静脉全血仪器法嗜酸性粒细胞分类计数及绝对值计数与手工分类法及目测法的测定结果无显著性差异, P 均>0.05,相关系数分别为0.964、0.997.结论 BC-5200全自动血细胞分析仪嗜酸性粒细胞计数结果可靠,可满足临床诊断和治疗需要.     

全自动血细胞分析仪嗜酸性粒细胞计数性能的评价

目的了解日本Sysmex XT-1800i全自动血细胞分析仪嗜酸性粒细胞计数结果的可靠性。方法随机取100份经EDTA-K2抗凝静脉全血,在Sysmex XT-1800i全自动血细胞分析仪上检测后制作成血涂片,经显微镜进行分类计数。同时用目测法进行嗜酸性粒细胞直接计数,将仪器分类嗜酸性粒细胞结果与手工分类法、仪器嗜酸性粒细胞绝对值计数结果与目测法结果分别进行比较。结果100份抗凝静脉全血仪器法嗜酸性粒细胞分类计数及绝对值计数与手工分类法及目测法的测定结果无显著性差异,P均>0.05,相关系数分别为0.964、0.997。结论Sysmex XT-1800i全自动血细胞分析仪嗜酸性粒细胞计数结果准确可靠,可满足临床诊断和治疗需要。     

全自动血细胞分析仪嗜酸性粒细胞计数性能的评价

目的了解日本Sysmex XT-1800i全自动血细胞分析仪嗜酸性粒细胞计数结果的可靠性。方法随机取100份经EDTA—K2抗凝静脉全血，在Sysmex XT-1800i全自动血细胞分析仪上检测后制作成血涂片。经显微镜进行分类计数。同时用目测法进行嗜酸性粒细胞直接计数。将仪器分类嗜酸性粒细胞结果与手工分类法、仪器嗜酸性粒细胞绝对值计数结果与目测法结果分别进行比较。结果100份抗凝静脉全血仪器法嗜酸性粒细胞分类计数及绝对值计数与手工分类法及目测法的测定结果无显著性差异，P均〉0．05，相关系数分别为0．964、0．997。结论Sysmex XT-1800i全自动血细胞分析仪嗜酸性粒细胞计数结果准确可靠，可满足临床诊断和治疗需要。     

Sysmex XE-2100全自动血液分析仪分析有核红细胞的临床应用评价

目的探讨 Sysmex XE-2100全自动血液分析仪有核红细胞计数的准确性并作评价.方法准确性测定分别用仪器法和显微镜目测法计数 38例抗凝静脉血的有核红细胞数( NRBC%),并将两者的结果进行比较.精密度测定选择 3份有核红细胞数值不同的抗凝静脉血分别用仪器法和目测法测定,每份标本重复计数 10次,各自计算出 CV值进行比较.结果 38份抗凝静脉血仪器法计数的有核红细胞结果与显微镜目测法计数结果之间无显著性差异,经 t检验, P >0.05,相关系数 r为 0.9893, P< 0.01.仪器法和显微镜目测法对 3份有核红细胞数值不同的标本重复计数的平均 CV值分别为 7.9%和 15.1%,显示出仪器法的精密度明显高于目测法.结论 Sysmex XE-2100全自动血液分析仪计数有核红细胞是一种较准确、快速、精密的方法,可满足临床诊断、治疗及疗效观察的需要.     

库尔特GEN-S血细胞分析仪VCS法与手工法测定嗜酸性粒细胞的比较

目的:应用库尔特GEN-S全自动血细胞分析仪的VCS法与常规手工计数法进行嗜酸性粒细胞计数,将两者结果进行比较.方法:随机抽取100例门诊病人的静脉血样本,分别用库尔特GEN-S全自动血细胞分析仪,手工乙醇-伊红法进行嗜酸性粒细胞计数.结果:手工法与VCS法计数结果差异无显著性(P>0.05),相关性良好,(r=0.98).但手工法计数结果变异系数较大(CV=10.65),重复性较仪器法差,而GEN-S血细胞分析仪重复性好(CV=5.56),操作简便,效率高.     

Beckman Coulter LH 750血液分析仪嗜酸性粒细胞计数性能评价

目的评价Beckman Coulter LH750型全自动血液分析仪嗜酸性粒细胞测定的临床应用价值。方法用LH 750型全自动血液分析仪、嗜酸性粒细胞直接计数法、白细胞分类计数法进行检测对比。精密度测定：分别用仪器法和目测法对3份不同嗜酸性粒细胞水平的样本进行计数,计算平均CV值进行比较。结果 195例对比实验结果表明,LH 750型全自动血液分析仪利用电阻抗、高频传导、激光散射（VCS原理）联合检测法对嗜酸性粒细胞的分析具有较好的准确性。仪器法与目测法对3份嗜酸性粒细胞不同水平的标本重复计数的批内平均CV值分别为3.88%和5.94%,显示出仪器法的精密度明显高于目测法。结论应用LH 750型全自动血液分析仪检测嗜酸性粒细胞有较高的准确性和精密度及较快的速度,与人工法比较差异不大,具有良好的相关性,可满足临床诊断、治疗及疗效观察的需要。     

BC-5500与手工显微镜计数嗜酸性粒细胞的比较

目的测定国产全自动五分类血细胞分析仪BC-5500计数嗜酸性粒细胞结果的准确性。方法随机抽取本院住院患者的静脉血80份,采用EDTA-K2抗凝,每份2ml。分别利用BC-5500和手工显微镜直接计数嗜酸性粒细胞。结果两种方法计数80份抗凝静脉血的嗜酸性粒细胞结果之间的差异无统计学意义（P〉0.05）。结论 BC-5500全自动血细胞分析仪性能可靠,嗜酸性粒细胞计数准确,大大缩短了检验时间,提高了工作效率,同时也可满足临床诊断、治疗和预后观察的需要。     

库尔特GEN-S血细胞分析仪VCS法与手工法测定嗜酸性粒细胞的比较

目的 :应用库尔特 GEN- S全自动血细胞分析仪的 VCS法与常规手工计数法进行嗜酸性粒细胞计数 ,将两者结果进行比较。方法 :随机抽取 10 0例门诊病人的静脉血样本 ,分别用库尔特 GEN- S全自动血细胞分析仪 ,手工乙醇 -伊红法进行嗜酸性粒细胞计数。结果 :手工法与 VCS法计数结果差异无显著性 (P>0 .0 5 ) ,相关性良好 ,(r=0 .98)。但手工法计数结果变异系数较大 (CV=10 .6 5 ) ,重复性较仪器法差 ,而 GEN- S血细胞分析仪重复性好 (CV=5 .5 6 ) ,操作简便 ,效率高     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.