曲马多加强针刺对关节炎大鼠的镇痛作用

目的 :观察不同剂量曲马多对关节炎大鼠热痛敏的镇痛作用及曲马多对针刺镇痛的影响。方法 :在完全弗氏佐剂关节炎大鼠模型上采用辐射热缩腿反射的方法 ,以痛敏分数作为观察指标 ,分别腹腔注射 (i.p .) 5mg·kg- 1 ·d- 1 、1 0mg·kg- 1 ·d- 1 、2 0mg·kg- 1 ·d- 1 曲马多和 1 0mL·kg- 1 ·d- 1 生理盐水 1 0天 ,记录每次给药 2 4hr后痛敏分数变化。继而选择 1 0mg·kg- 1 ·d- 1 曲马多与电针合用 ,观察单独应用 1 0mg·kg- 1 ·d- 1 曲马多、单独应用电针、曲马多与电针合用及1 0mL·kg- 1 ·d- 1 生理盐水四组 (均为 1 0天 ) ,每次给药或 /和电针 2 4hr后痛敏分数变化。结果 :i.p .5mg·kg- 1 ·d- 1 曲马多连续应用 1 0天时 ,大鼠痛敏分数无明显变化 ;i.p.1 0mg·kg- 1 ·d- 1 曲马多 9天时大鼠痛敏分数显著提高 ;i.p .2 0mg·kg- 1 ·d- 1 曲马多 6天时大鼠的痛敏分数显著提高 ;单独应用电针 8天时大鼠痛敏分数显著提高 ;i.p.1 0mg·kg- 1 ·d- 1 曲马多与电针合用 4天时可明显提高大鼠痛敏分数。结论 :长期应用曲马多对慢性炎症痛大鼠具有镇痛作用 ,呈剂量相关性 ;曲马多与电针合用可加强电针的镇痛作用 ,并降低曲马多使用剂量。提示曲马多与电针合用是一种治疗慢性痛的有效方法     

口服猪脾来源免疫抑制组分抗炎作用的实验研究

 目的研究口服Mr小于5000的猪脾来源免疫抑制组分（SISC-p）的抗炎作用。方法通过给动物ig SISC-p,观察其对巴豆油致小鼠耳廓肿胀和对大鼠棉球肉芽肿的影响。结果ig SISC-p一次给药对巴豆油致小鼠耳廓肿胀没有影响,多次给药在一定剂量（10mg·kg^-1）则有显著的抑制作用（P＜0.05）;在实验剂量（16,8和4 mg·kg^-1）下,SISC-p对大鼠棉球肉芽肿均有显著的抑制作用,其中高剂量和中剂量的作用效果较好。结论ig SISC-p多次给药对急性炎症和亚急性炎症均有对抗作用。     

氯沙坦和卡托普利联合应用对高血压大鼠肾脏的保护作用

目的　探讨氯沙坦和卡托普利联合应用对自发性高血压大鼠 (SHR)肾结构与功能的保护作用。方法　将自发性高血压大鼠 30只随机分为对照组和 3个治疗组 ,治疗组分别以氯沙坦 (30mg·kg- 1 ·d- 1 )、卡托普利 (1 0 0mg·kg- 1 ·d- 1 )、卡托普利50mg·kg- 1 ·d- 1 +氯沙坦 1 5mg·kg- 1 ·d- 1 灌胃 1 5周 ,观察大鼠的血压、尿蛋白 ,肾组织中血管紧张素Ⅱ (AngⅡ )、转化生长因子 β1 (TGFβ1 )以及肾组织病理改变。结果　氯沙坦和卡托普利治疗组的尿蛋白显著减少 ,肾内Ⅳ型胶原沉积和TGFβ1 减少 ,肾小动脉结构正常 ,且氯沙坦 +卡托普利治疗作用更显著。结论　氯沙坦合用卡托普利后具有协同作用 ,可以延缓肾脏损害。     

广西沿海平颏海蛇和青环海蛇提取物的药效学研究

目的 观察广西沿海平颏海蛇和青环海蛇(1:1)提取物的主要药效学作用.方法 采用佐剂致大鼠关节炎、巴豆油致小鼠耳肿胀、醋酸所致小鼠腹腔毛细血管通透性增高及棉球致大鼠肉芽组织增生、醋酸致小鼠疼痛等模型,观察广西沿海平颏海蛇和青环海蛇(1:1)提取物的药效学作用.结果 广西沿海平颏海蛇和青环海蛇提取物能明显抑制佐剂致大鼠关节炎、亦能明显抑制巴豆油所致的小鼠耳肿胀和醋酸所致小鼠腹腔毛细血管通透性增高,对棉球致大鼠肉芽组织增生有明显抑制作用,并有显著的镇痛作用.结论 广西沿海平颏海蛇和青环海蛇提取物具有抗风湿、抗炎、镇痛作用.     

雪上一枝蒿总碱注射液的药效学研究

目的：对雪上一枝蒿总碱注射液的抗炎、镇痛作用进行研究。方法：采用巴豆油所致小鼠耳廓肿胀法和角又菜胶所致大鼠足跖肿胀法，观察抗炎作用；采用小鼠热板法和甲醛所致小鼠疼痛模型，观察镇痛作用。结果：雪上一枝蒿总碱注射液可明显抑制巴豆油所致小鼠耳廓肿胀和角叉莱胶所致大鼠足跖肿胀，能明显提高热板法小鼠痛阈，对甲醛疼痛模型小鼠舔足反应具有明显的抑制作用。结论：雪下一枝蒿总碱注射液具有明显的抗炎、镇痛作用。     

铁包金不同萃取物的抗炎镇痛作用研究

目的：观察铁包金提取物的镇痛抗炎作用。方法：铁包金提取物按0．2g/kg和0．4gg/kg的剂量对巴豆油所导致的小鼠出现耳廓肿胀以及由醋酸所致的小鼠扭体分别进行实验。结果：铁包金中的提取物样品可以对因为巴豆油而导致的小白鼠的耳廓肿胀有明显的抑制作用;对醋酸引起的小鼠扭体的次数有了明显的减少。结论：铁包金的提取物具有镇痛抗炎作用。     

龙眼肉乙醇提取物对雌性大鼠垂体-性腺轴的作用

目的 :观察龙眼肉乙醇提取物对大鼠垂体 -性腺轴的作用。方法 :龙眼肉乙醇提取物给大鼠ip。 2 5g·kg- 1 ·d- 1 、5g·kg- 1 ·d- 1 ,共 1 0天 ,颈总动脉取血 ,用放射免疫法测定大鼠血浆中FSH、E2 、P、LH、T、PRL的含量。结果 :龙眼肉乙醇提取物明显降低雌性大鼠血浆中PRL、E2 、T的含量 (P <0 0 1 ) ,增加其FSH、P的含量 (P <0 0 1 )。但是对LH的含量无影响。结论 :龙眼肉乙醇提取物明显影响大鼠垂体 -性腺轴的内分泌机能。     

参莲提取物抗炎药理活性评价

目的 评价参莲提取物的抗炎作用.方法 采用巴豆油诱导小鼠急性耳肿胀模型、大鼠慢性肉芽肿模型、脂多糖(lipopolysaccharide,LPS)诱导小鼠急性肺炎性损伤模型、家兔内皮剥脱复合高脂诱导动脉粥样硬化(atherosclerosis,AS)早期炎症损伤模型等,对中药复方制剂参莲提取物进行抗炎药理作用评价.结果 参莲提取物可抑制大鼠肉芽肿生成,降低家兔AS早期炎症反应,对小鼠急性肺炎症损伤反应有一定的抑制作用,对巴豆油诱导小鼠急性耳肿胀无明显治疗作用.结论 参莲提取物具有明显的抗炎作用,对慢性、亚急性炎症的治疗作用优于急性炎症.     

净得康喷雾剂的抗菌、止痒、抗炎作用研究

目的:观察净得康喷雾剂的主要药效学作用。方法:采用体外抗菌;细菌致大鼠阴道炎模型、霉菌致大鼠阴道炎模型,取造模成功的大鼠随机分为4组,每组10只。其中2个组分别阴道给予净得康喷雾剂生药2.0,0.5 g·kg-1(给药体积为1 mL.kg-1);1个组阴道给予妇炎平胶囊0.5 g·kg-1;余1组为模型对照,阴道给予等体积蒸馏水。磷酸组织胺致豚鼠瘙痒、巴豆油所致小鼠耳壳肿胀等模型,分组方式同上,采用外用药给药方式,其中巴豆油所致耳壳肿胀模型小鼠涂抹给予净得康喷雾剂生药10.0,2.5 g·kg-1,观察净得康喷雾剂的主要药效学作用。结果:净得康喷雾剂对阴道感染常见的细菌具有不同程度的抑制作用;对大鼠细菌性阴道炎的抑制率为82.6%、霉菌性阴道炎的治愈率为90%;并能明显抑制磷酸组织胺所致豚鼠瘙痒反应,抑制率为51.2%;对巴豆油诱发小鼠耳廓肿胀的抑制率为15.8%。结论:净得康喷雾剂具有抗菌、止痒、抗炎作用。     

昆明山海棠总生物碱提取新工艺的研究

目的昆明山海棠总生物碱提取新工艺的研究。方法采用大孔吸附树脂法。结果与结论用0.5%酸水提取,H型大孔吸附树脂吸附昆明山海棠总生物碱,提取量可以达到500mg·kg-1,生物碱在50,25mg·kg-1对正常大鼠棉球肉芽肿有明显的抑制作用(P<0.001),对角叉菜胶致正常大鼠足跖肿胀在6h后有抑制作用。     

脊痛宁胶囊抗炎镇痛作用的实验研究

目的研究脊痛宁胶囊的抗炎、镇痛作用。方法以巴豆油致小鼠耳肿胀模型和角叉菜胶致大鼠足肿胀模型,观察脊痛宁胶囊的急性抗炎作用;以棉球植入小鼠腹腔致肉芽肿模型,观察脊痛宁胶囊的慢性抗炎作用;以醋酸法造成小鼠疼痛模型,观察脊痛宁胶囊的镇痛作用。结果脊痛宁胶囊对巴豆油致耳肿胀和角叉菜胶致大鼠足肿胀有显著的抑制作用,可显著抑制棉球所致肉芽肿形成,减少醋酸所致小鼠扭体次数。结论脊痛宁胶囊具有显著的抗炎、镇痛作用。     

Assessment of anti-inflammatory and antinociceptive activities of Daphne pontica L. (Thymelaeaceae)

The n-hexane, diethylether, ethyl acetate and methanol extracts from roots, leaves, stems and flowers with young leaves of Daphne pontica L. (Thymelaeaceae) were investigated for their in vivo anti-inflammatory and antinociceptive activities. For the anti-inflammatory activity assessment, carrageenan-induced hind paw edema, PGE(2)-induced hind paw edema and 12-O-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema models and for the antinociceptive activity, p-benzoquinone-induced abdominal constriction test were used. Only ethyl acetate extracts of the roots showed significant anti-inflammatory activity on carrageenan-induced (22.7-32.0% inhibition) and PGE(2)-induced hind paw edema (3.2-27.3% inhibition) as well as 12-O-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema (47.8-43.3% inhibition) models at 50 mg/kg dose without inducing any apparent gastric lesion or acute toxicity, whereas the other extracts were shown to be ineffective. In addition to roots, ethyl acetate extracts of the stems exhibited 19.5-29.9%; 5.3-23.9%; 36.6-28.1% inhibition on carrageenan-induced and PGE(2)-induced hind paw edema as well as 12-O-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema models, respectively. On the other hand, none of the extracts showed any significant antinociceptive activity.     

Anti-inflammatory activity of Jatropha curcas roots in mice and rats

Anti-inflammatory activity of topical application of Jatropha curcas L. root powder in paste form in TPA-induced ear inflammation was confirmed in albino mice and the successive solvent extraction of these roots was carried out by ether and methanol. The methanol extract exhibited systemic and significant anti-inflammatory activity in acute carrageenan-induced rat paw edema. It also showed activity against formalin-induced rat paw edema, as well as, turpentine-induced exudative changes and cotton pellet-induced granular tissue formation after oral treatment for 7 days in albino rats. Thus, resultant anti-inflammatory activity might be due to effects on several mediators and arachidonic acid metabolism involving cyclo-oxygenase pathway resulting in prostaglandin formation, anti-proliferative activity leading to reduction in granular tissue formation and leukocyte migration from the vessels.     

In vivo anti-inflammatory and antinociceptive actions of some Lamium species

ETHNOPHARMACOLOGICAL RELEVANCE: Several Lamium species have been used to relieve pain in arthritic ailments in Turkish folk medicine. AIM OF THE STUDY: To evaluate the anti-inflammatory and antinociceptive activities of different extracts prepared with methanol, dichloromethane, n-butanol and water from the aerial parts of some Lamium species of Turkish origin. MATERIALS AND METHODS: Lamium eriocephalum subsp. eriocephalum, Lamium garganicum subsp. laevigatum, Lamium garganicum subsp. pulchrum and Lamium purpureum var. purpureum (Lamiaceae); for the anti-inflammatory activity, carrageenan-induced hind paw edema model, PGE(2)-induced hind paw edema model, and 12-O-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema model and for the antinociceptive activity p-benzoquinone (PBQ)-induced writhing test in mice were employed. RESULTS: The n-butanol extracts of Lamium garganicum subsp. laevigatum (LGL-BuOH), Lamium garganicum subsp. pulchrum (LGP-BuOH), and Lamium purpureum var. purpureum (LPP-BuOH) exhibited notable inhibition (16.5-28.9, 14.5-26.9, 12.3-21.5%, resp.) in carrageenan-induced hind paw edema model at doses of 200mg/kg without inducing any gastric damage. The LGL-BuOH (7.1-30.4%) and LGP-BuOH (5.9-24.1%) extracts also displayed potent anti-inflammatory activity against PGE(2)-induced hind paw edema model. LGL-BuOH and LGP-BuOH were also found to exhibit remarkable antinociceptive activity in p-benzoquinone-induced abdominal constriction test at a dose of 200 mg/kg (25.0 and 24.3%, respectively). CONCLUSION: The experimental data demonstrated that Lamium garganicum subsp. laevigatum and Lamium garganicum subsp. pulchrum displayed remarkable anti-inflammatory and antinociceptive activities.     

栽培黄芩和野生黄芩抗炎、解热和免疫作用比较研究

目的:探讨栽培黄芩和野生黄芩的抗炎、解热作用和对非特异性免疫功能的影响,并比较它们的药效。方法:以巴豆油致小鼠耳肿胀法和醋酸致小鼠腹腔毛细血管渗出法比较栽培黄芩与野生黄芩的抗炎作用;以干酵母致大鼠发热法比较其解热作用;采用碳廓清实验比较其对非特异性免疫功能的影响。结果:栽培黄芩和野生黄芩均能明显抑制巴豆油致小鼠耳肿胀和小鼠腹腔毛细血管通透性增加、降低干酵母致大鼠发热模型的体温、提高小鼠碳廓清指数(K)值和吞噬指数(α)值。结论:栽培黄芩和野生黄芩都具有抗炎、解热作用,可提高非特异性免疫功能。药效学比较两者无显著性差异,为栽培黄芩代替野生黄芩提供了实验依据。     

丹芷胶囊抗炎镇痛作用的实验研究

目的:评价丹芷胶囊的抗炎、镇痛作用。方法:用二甲苯致小鼠耳廓肿胀试验、角叉菜胶致大鼠足跖肿胀试验,考察丹芷胶囊的抗炎作用;用小鼠醋酸扭体法和热板法,考察丹芷胶囊的镇痛作用。结果:丹芷胶囊能明显抑制二甲苯致小鼠耳廓肿胀和角叉菜胶致大鼠足跖肿胀;对醋酸致小鼠扭体反应有明显的抑制作用,提高小鼠热痛阈值。结论:丹芷胶囊有明显的抗炎、镇痛作用。     

Anti-inflammatory and related pharmacological activities of the n-BuOH subfraction of mushroom Phellinus linteus

This study aimed to elucidate the anti-inflammatory and related activities of mushroom Phellinus linteus. The results show that the EtOH extract of Phellinus linteus (PLE) dose-dependently inhibited the mouse ear edema induced by croton oil. Among PLE subfractions, the n-BuOH subfraction showed highest anti-inflammatory activity in croton oil-induced ear edema test. The n-BuOH subfraction also showed highest inhibitory activity on the chick embryo chorioallantoic membrane (CAM) angiogenesis in a dose-dependent manner. PLE could significantly reduce the number of writhing induced by acetic acid in mice, indicating that PLE possesses potent antinociceptive effect mediated by its anti-inflammatory activity. Mycelial extract of six different Phellinus strains were found to contain anti-angiogenic activity in the CAM assay. These results suggest that Phellinus linteus has anti-inflammatory and antinociceptive activities, in addition to its anti-angiogenic activity.     

Anti-inflammatory activity of Sedum kamtschaticum

Sedum kamtschaticum Fischer (Crassulaceae) has been used as a folk medicine in North-East Asia for treating inflammatory disorders. The present investigation was carried out to establish in vivo anti-inflammatory activity and cyclooxygenase-2 modulating activity of this plant material. The methanol extract of Sedum kamtschaticum significantly inhibited mouse croton oil-induced ear edema (24–47% inhibition at 50–400 mg/kg) and rat paw edema (24–30% inhibition at 400–800 mg/kg) by oral administration. Prednisolone (10 mg/kg) showed 54 and 36% inhibition in the same animal models, respectively. Sedum kamtschaticum also showed significant inhibitory activity against mouse ear edema induced by multiple treatment of phorbol ester for 3 days. In addition, Sedum kamtschaticum exhibited potent analgesic activity against mouse acetic acid-induced writhing (IC₅₀=125 mg/kg), while aspirin (200 mg/kg) showed 57% inhibition. Using lipopolysaccharide-treated RAW 264.7 cells, down-regulation of cyclooxygense-2 expression was found to be one of the cellular action mechanisms of anti-inflammation by Sedum kamtschaticum.     

炎康颗粒的抗炎作用及机制研究

目的研究炎康颗粒的抗炎作用，并探讨其作用机制。方法采用巴豆油致小鼠耳肿胀实验模型、角叉菜胶致雄性大鼠足跖肿胀实验模型，观察炎康颗粒对急性炎症的作用；采用大鼠棉球肉芽肿实验模型，观察炎康颗粒对慢性炎症的作用；采用摘除双侧肾上腺的大鼠进行角叉菜胶致足跖肿胀实验及棉球肉芽肿实验，以观察炎康颗粒抗炎作用与下丘脑-垂体-肾上腺轴（HPAA）的关系。结果炎康颗粒8、16g（生药）／kg连续给药1周，明显抑制小鼠耳肿胀；1.8g（生药）／kg明显抑制由角叉菜胶诱导的大鼠足趾肿胀；明显抑制大鼠棉球肉芽的增生。摘除肾上腺后，炎康颗粒连续给药1周，无明显抗炎作用。结论炎康颗粒对急性炎症和慢性炎症均有明显的防治作用，其抗炎作用依赖于HPAA系统，并与减少白细胞介素有关。     

Anti-inflammatory and antinociceptive potential of Maclura pomifera (Rafin.) Schneider fruit extracts and its major isoflavonoids, scandenone and auriculasin

The aqueous, ethanolic and chloroform extracts and two prenylated isoflavones: scandenone (I) and auriculasin (II), isolated from the fruits of Maclura pomifera (Rafin.) Schneider, were investigated for their in vivo anti-inflammatory and antinociceptive activity. For the anti-inflammatory activity, both carrageenan-induced hind paw edema and 12-O-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema models and for the antinociceptive activity, p-benzoquinone-induced abdominal constriction test were used. Scandenone, the chloroform and the ethanolic extracts were shown to possess antinociceptive activity and anti-inflammatory activity on carrageenan-induced hind paw edema model at 100 mg/kg dose. The same compound and the extract were also found to be highly active in (TPA)-induced mouse ear edema model whereas auriculasin and the H(2)O extract showed to be inactive in all of the assays.