抗癌生物活性肽对人乳腺癌nm231细胞的增殖抑制作用

 目的 探讨抗癌生物活性肽（ACBP）对人乳腺癌 nm231细胞的作用及其机制。 方法 nm231 细胞经不同浓度（0.05、0.10、0.20、 0.25 µg/ml）ACBP 作用 72 h，以噻唑蓝（MTT）法测定细胞增殖活性。以 0.25 µg/ml 的 ACBP 分别作用于 nm231 细胞 4、6、24、48、72 h，行光镜和透射电镜（作用 48 h 细胞）观察。应用 DNA 凝胶电泳和磷脂结合蛋白 V（Annexin V，AV）/碘化丙啶（PI）双标记染色流式细胞术等方法，观察 0.25 µg/ml 的 ACBP 作用不同时间对nm231 细胞凋亡发生及作用 48 h 对细胞周期的影响。以上各项检测均设以 RPMI 1640 培养液取代 ACBP 的对照组。 结果 浓度为 0.1 µg/ml 的 ACBP 即对 nm231 细胞的增殖有明显抑制作用，抑制率为 10.3%，抑制作用具有浓度依赖性，ACBP 浓度为 0.25 µg/ml 时抑制率达 35.1%。光镜观察显示，经 0.25 µg/ml 的 ACBP 作用 24 h，细胞出现凋亡特征；作用 48 h，光镜及电镜下均可见大量的典型凋亡细胞。DNA 凝胶电泳显示，经 0.25 µg/ml 的ACBP 作用 24 h 的细胞出现 DNA 断裂；作用 48 h 的细胞出现典型的梯形电泳图谱。流式细胞术分析显示，ACBP 作用后AV（+）/ PI（－）细胞和 AV（+）/ PI（+）细胞比例均随培养时间延长而增大；作用 48 h 的 nm231细胞 G0/1 期细胞比例高于对照组（P < 0.01），而细胞增殖指数低于对照组（P < 0.01）。 结论 ACBP 可抑制 nm231 细胞的增殖，其机制与抑制nm231 细胞的 DNA 合成和诱导细胞凋亡相关。     

抗癌胶囊对实验性肿瘤的治疗及对化疗减毒作用研究

目的：研究抗癌胶囊对小鼠移植肿瘤S180，H22，Lewis的抑瘤作用及对化疗药所致小鼠免疫功能抑制的保护作用。方法：按照抗癌药物筛选规程进行体内抑瘤实验：采用氟尿嘧啶（FU）制备免疫功能低下模型，测定抗癌胶囊对小鼠白细胞计数、免疫器官重量、腹腔巨噬细胞吞噬功能和NK细胞的影响。结果：抗癌胶囊对小鼠移植肿瘤S180，H22，Lewis均有不同的抑瘤作用，其中高剂量组抑瘤效果最佳，并对小鼠体重生长无明显影响。抗癌胶囊还有明显拮抗FU所致白细胞下降，胸腺、脾脏萎缩，腹腔巨噬细胞功能降低和NK细胞减少等毒副作用。结论：抗癌胶囊具有明显的抗肿瘤作用。     

抗癌口服液在体内外抗肿瘤作用的实验研究

OBJECTIVETo investigate anti-cancer of the Anti-cancer Oral Liquid (ACOL) for MGC-803 gastric cancer cell line in vivo and vitro. METHODUsing the MTT assay, colonogenic assay and sero-pharmacological experiment by treating with the Chinese traditional med     

人肺癌细胞体外对抗癌药物敏感性的初探

目的探讨人肺癌细胞体外对１０种抗癌药的敏感性及方法的可靠性。方法收集人肺癌手术切除的标本２０例，用一简单、快速，有一定特异性优点的ＭＴＴ（３－（４，５）－双甲基－２－唑噻－（２，５）－二苯基溴化四氮唑蓝）法作威猛、足叶乙甙、阿霉素、长春新碱、长春地辛、卡铂、平阳霉素、氟脲嘧啶、环磷酰胺和异环磷酰胺抗癌敏感药物试验。结果患者不同个体之间药物敏感性不同（Ｐ＜０．０１）：１０种药物平均抑制率以威猛类药物最高（Ｐ＜０．０１），依次为阿霉素、卡铂、足叶乙甙、长春地辛等。敏感药物顺序与临床治疗结果大致相符。结论用ＭＴＴ法可作肿瘤治疗个案化较为可靠的检测方法。     

调节p53途径—提高癌症治疗效率

p53肿瘤抑制蛋白,也被称为基因组监控因子,是一种保护细胞免受一系列生理逆境(例如致癌基因活化、辐射、有丝分裂压力、核糖体压力和化学损害)的转录控制因子.这些生理逆境会导致依赖于p53激活的信号产生,并通过复杂的相互作用网络定位到细胞核,起始转录或抑制许多基因的表达,这些基因与诱导生长停滞、修复、细胞凋亡、衰老或者改变新陈代谢有关.由于p53途径对于调控疾病有如此重要的作用,使得针对该途径的药物干预日益成为人们关注的焦点.本文对调节p53功能的最新研究进展进行综述,并对有关p53基因的癌症治疗前景进行展望.     

Pro-Apoptotic Activity of 4-Isopropyl-2-(1-Phenylethyl) Aniline Isolated from Cordyceps bassiana

Cordyceps species including Cordyceps bassiana are a notable anti-cancer dietary supplement. Previously, we identified several compounds with anti-cancer activity from the butanol fraction (Cb-BF) of Cordyceps bassiana. To expand the structural value of Cb-BF-derived anti-cancer drugs, we employed various chemical moieties to produce a novel Cb-BF-derived chemical derivative, KTH-13-amine-monophenyl [4-isopropyl-2-(1-phenylethyl) aniline (KTH-13-AMP)], which we tested for anti-cancer activity. KTH-13-AMP suppressed the proliferation of MDA-MB-231, HeLa, and C6 glioma cells. KTH-13-AMP also dose-dependently induced morphological changes in C6 glioma cells and time-dependently increased the level of early apoptotic cells stained with annexin V-FITC. Furthermore, the levels of the active full-length forms of caspase-3 and caspase-9 were increased. In contrast, the levels of total forms of caspases-3, caspase-8, caspase-9, and Bcl-2 were decreased in KTH-13-AMP treated-cells. We also confirmed that the phosphorylation of STAT3, Src, and PI3K/p85, which is linked to cell survival, was diminished by treatment with KTH-13-AMP. Therefore, these results strongly suggest that this compound can be used to guide the development of an anti-cancer drug or serve as a lead compound in forming another strong anti-proliferative agent.     

Thymoquinone (2-Isopropyl-5-methyl-1, 4-benzoquinone) as a chemopreventive/anticancer agent: Chemistry and biological effects

Cancer remains the topmost disorder of the mankind and number of cases is unceasingly growing at unprecedented rates. Although the synthetic anti-cancer compounds still hold the largest market in the modern treatment of cancer, natural agents have always been tried and tested for potential anti-cancer properties. Thymoquinone (TQ), a monoterpene and main ingredient in the essential oil of Nigella sativa L. has got very eminent rankings in the traditional systems of medicine for its anti-cancer pharmacological properties. In this review we summarized the diverse aspects of TQ including its chemistry, biosynthesis, sources and pharmacological properties with a major concern being attributed to its anti-cancer efficacies. The role of TQ in different aspects involved in the pathogenesis of cancer like inflammation, angiogenesis, apoptosis, cell cycle regulation, proliferation, invasion and migration have been described. The mechanism of action of TQ in different cancer types has been briefly accounted. Other safety and toxicological aspects and some combination therapies involving TQ have also been touched. A detailed literature search was carried out using various online search engines like google scholar and pubmed regarding the available research and review accounts on thymoquinone upto may 2019. All the articles reporting significant addition to the activities of thymoquinone were selected. Additional information was acquired from ethno botanical literature focusing on thymoquinone. The compound has been the centre of attention for a long time period and researched regularly in quite considerable numbers for its various physicochemical, medicinal, biological and pharmacological perspectives. Thymoquinone is studied for various chemical and pharmacological activities and demonstrated promising anti-cancer potential. The reviewed reports confirmed the strong anti-cancer efficacy of thymoquinone. Further in-vitro and in-vivo research is strongly warranted regarding the complete exploration of thymoquinone in ethnopharmacological context.     

Natural products against cancer: Review on phytochemicals from marine sources in preventing cancer

Marine natural products have as of now been acknowledged as the most important source of bioactive substances and drug leads. Marine flora and fauna, such as algae, bacteria, sponges, fungi, seaweeds, corals, diatoms, ascidian etc. are important resources from oceans, accounting for more than 90% of the total oceanic biomass. They are taxonomically different with huge productive and are pharmacologically active novel chemical signatures and bid a tremendous opportunity for discovery of new anti-cancer molecules. The water bodies a rich source of potent molecules which improve existence suitability and serve as chemical shield against microbes and little or huge creatures. These molecules have exhibited a range of biological properties antioxidant, antibacterial, antitumour etc. In spite of huge resources enriched with exciting chemicals, the marine floras and faunas are largely unexplored for their anticancer properties. In recent past, numerous marine anticancer compounds have been isolated, characterized, identified and are under trials for human use. In this write up we have tried to compile about marine-derived compounds anticancer biological activities of diverse flora and fauna and their underlying mechanisms and the generous raise in these compounds examined for malignant growth treatment in the course of the most recent quite a long while.     

Apoptogenic activity of ethyl acetate extract of leaves of Memecylon edule on human gastric carcinoma cells via mitochondrial dependent pathway

ObjectiveTo evaluate the anti-proliferative and apoptogenic activity of ethyl acetate extract from the leaves of Memecylon edule (EtAc-LME) in MKN-74, NUGC gastric cancer cells and non cancerous gastric mucous cells (GES-1), and to explore the mechanism of EtAc-LME induced apoptosis.MethodsThe mechanism of EtAc-LME induced apoptosis was explored by analysing the activation of pro-caspases, PARP cleavage, expression of cytochrome-c (Cyt-c) was determined by western blotting, mRNA expression of Bcl-2, Bax by RT-PCR, loss of mitochondrial potential using DiOC6 dye, annexin binding assay and its influence on cell cycle arrest by flow cytometry.ResultsThe results indicated that EtAc-LME inhibited the gastric cancer cell growth in dose-dependent manner and cytotoxicity was more towards the gastric cancer cells (NUGC and MKN-74) compared to normal gastric cells (GES-1), suggesting more specific cytotoxicity to the malignant cells. Over expression of Cyt-c and subsequent activation of caspases-3 and down regulation of Bcl-2 and loss in mitochondrial potential in EtAc-LME treated MKN-74 and NUGC cells suggested that EtAc-LME induced apoptosis by mitochondrial dependent pathway.ConclusionsThe present findings suggest that ethyl acetate extract of Memecylon edule induces apoptosis selectively in gastric cancer cells emphasizing the importance of this traditional medicine for its potential in the treatment of gastric cancer.     

EGF-IL-18 fusion protein as a potential anti-tumor reagent by induction of immune response and apoptosis in cancer cells

We report here the generation and characterization of EGF-IL-18 fusion protein as an anti-tumor reagent. The epidermal growth factor (EGF) and interleukin-18 (IL-18) fusion protein was shown to induce interferon-γ (IFNγ) expression and secretion in KG-1 cells, and to promote PBMNC proliferation. It also stimulated activation of CD4⁺ T cells, and increased other immune responses. Moreover, EGF-IL-18 could induce significant tumor regression in SMMC-7721-xenografted Balb/c nude mice when administered together with peritumoral injection of X-ray-irradiated NK-92 cells, and this regression is associated with arresting of the tumor cells in G1 phase and induction of apoptosis.