齐多夫啶半乳糖神经酰胺脂质体的制备及理化性质研究

目的 制备齐多夫啶半乳糖神经酰胺脂质体并对其理化性质进行研究。方法 采用两次乳化法，以半乳糖神经酰胺为主要膜材制备齐多夫啶半乳糖神经酰胺脂质体并进行形态学观察、检测其包封率和稳定性。结果 透射电子显微镜下观察齐多夫啶半乳糖神经酰胺脂质体为粒径均匀的球形或近球形的小单层颗粒；粒径范围为60～270nm，D50为140nm；包封率达72．5％，但10d后快速下降。结论 齐多夫啶半乳糖神经酰胺脂质体制备方法可行，需进一步提高包封率和稳定性。     

中枢性高热头部降温病人体温测量方法

介绍了中枢性高热的特点、头部物理降温的意义及监测体温的部位和方法。认为耳温测量是中枢性高热头部降温病人最理想的测温方法，测温快速、准确、安全、省时。     

黄芩苷磷脂复合物理化性质的研究

 目的考察黄芩苷与磷脂形成复合物后的理化性能变化。方法对研制的黄芩苷磷脂复合物的晶体特征、相变特征、水分散性质、溶解性能及油水分配系数进行了研究。结果射线衍射分析显示,复合物呈现无定型特征;差示扫描显示复合物的相变温度降低;还观察到复合物在水中分散呈胶团状,与脂质体类似;黄芩苷磷脂复合物明显改善了黄芩苷在水及正辛醇中的溶解性能;在不同pH水正辛醇系统中,黄芩苷磷脂复合物的表观油水分配系数与黄芩苷相比也有较大的差异。结论与黄芩苷相比,黄芩苷磷脂复合物的理化性质有较显著的改变。     

理化处理对啤酒酵母自溶的影响

利用酵母自溶动力学方程考察了温度、破壁酶、酵母预处理、酵母初始质量分数、盐等理化因素对啤酒酵母自溶过程的影响.温度对酵母自溶速度有很大影响.通过回归分析得到不同体系中反映自溶温度和速度常数关系的Arrhenius方程及相应的自溶反应的活化能Ea；破壁预处理能显著加速酵母自溶，添加破壁复合酶或食盐均有利于加速酵母自溶反应，但两者复合添加有相互抑制作用；酵母初始质量分数对自溶影响不大.     

Cytotoxicity of carbon nanotube variants: A comparative in vitro exposure study with A549 epithelial and J774 macrophage cells

Abstract

While production of engineered carbon nanotubes (CNTs) has escalated in recent years, knowledge of risk associated with exposure to these materials remains unclear. We report on the cytotoxicity of four CNT variants in human lung epithelial cells (A549) and murine macrophages (J774). Morphology, metal content, aggregation/agglomeration state, pore volume, surface area and modifications were determined for the pristine and oxidized single-walled (SW) and multi-walled (MW) CNTs. Cytotoxicity was evaluated by cellular ATP content, BrdU incorporation, lactate dehydrogenase (LDH) release, and CellTiter-Blue (CTB) reduction assays. All CNTs were more cytotoxic than respirable TiO₂ and SiO₂ reference particles. Oxidation of CNTs removed most metallic impurities but introduced surface polar functionalities. Although slopes of fold changes for cytotoxicity endpoints were steeper with J774 compared to A549 cells, CNT cytotoxicity ranking in both cell types was assay-dependent. Based on CTB reduction and BrdU incorporation, the cytotoxicity of the polar oxidized CNTs was higher compared to the pristine CNTs. In contrast, pristine CNTs were more cytotoxic than oxidized CNTs when assessed for cellular ATP and LDH. Correlation analyses between CNTs’ physico–chemical properties and average relative potency revealed the impact of metal content and surface area on the potency values estimated using ATP and LDH assays, while surface polarity affected the potency values estimated from CTB and BrdU assays. We show that in order to reliably estimate the risk posed by these materials, in vitro toxicity assessment of CNTs should be conducted with well characterized materials, in multiple cellular models using several cytotoxicity assays that report on distinct cellular processes.     

甘草酸及其脂质配位体的理化性质与抗肝损害作用的比较

目的 :增加甘草酸的保肝作用。方法 :在隋性溶剂中将甘草酸与磷脂酰胆碱形成配位体口服。结果 :甘草酸形成脂质配位体后从水溶性变为脂溶性 ,理化性质 (红外、紫外光谱、高效液相等 )无明显改变 ,抗肝损作用明显增加。结论 :口服甘草酸脂质配位体的抗肝损作用明显优于甘草酸。     

CGF-1型光固化复合体的研制

目的　研制一种新型复合体 ,测定 CGF- 1,Dyract,Elan复合体和 Fuji II玻璃离子的物理机械性能 .方法　按照有关的试验方法检测这些材料的径向拉伸强度 (DTS)、挠曲强度 (FS)、压缩强度 (CS )、表面硬度 (Hv)、固化深度 (CD)、磨耗率 (R)、吸水值 (Wsp)和水溶解值 (Wsl) .结果　 CGF- 1的 CD值最高 ,R值最低 (P<0 .0 1) ;DTS,FS,Hv,CS低于Dyract和 Elan(P<0 .0 5 ) ;Fuji II玻璃离子的 Wsp值大大高于所有复合体 (P<0 .0 1) ;CGF- 1和 Elan的 Wsl值有明显差异 (P<0 .0 1) ,CGF- 1的 Wsl值最低 .结论　 CGF- 1型复合体具有较高的固化深度、优异的耐磨性和中等机械强度 ,是一种特别适合用于非咬合应力区域的新型充填修复材料