p16INK4a免疫细胞化学法对子宫颈癌筛查价值的Meta分析#br#

Objective?To use evidence-based medicine to comprehensively evaluate two methods in cervical cancer screening: Thinprepliquid based cytology test (TCT) and p16INK4a Immunocytochemistry (p16INK4a ICC). Methods?Pubmed, Google scholar, cochrane clinical trial, China National Knowledge Network, Wanfang Medical Network and other databases were searched, and literatures related to p16INK4a ICC and TCT screening for cervical cancer were included.The quality of the included literature was evaluated, and Stata16.0 software was used for meta analysis. Results?Finally, 10 articles were included with a total of 2 694 patients. The combined sensitivity of TCT screening and p16INK4a ICC screening was 86% (95%CI: 77%~92%) and 90% (95%CI: 81%~95%), the combined specificity was 64% (95%CI: 48%~78%) and 79% (95%CI: 69%~86%), and the combined positive likelihood ratio was 2.4 (95%CI: 1.6~3.6) and 4.2 (95%CI: 2.8~6.3), the combined negative likelihood ratio was 0.21 (95%CI: 0.13~0.34) and 0.13 (95%CI: 0.06~0.25), combined diagnostic odds ratio (DOR) was 11 (95%CI: 6~22) and 33 (95%CI: 13~85), and the area under the receiver operating characteristic curve (SROC) (AUC) was 0.85 and 0.91, respectively. Conclusions?The application of p16INK4a ICC test used in cervical cancer screening has higher sensitivity and specificity than TCT, which helps to improve the accuracy of cervical cancer screening and avoid missed diagnosis.     

INHBA在结直肠癌中的表达及其与微卫星状态和临床病理特征的关系

目的探讨INHBA在结直肠癌中的表达及其与微卫星状态、临床病理特征之间的关系。方法基于基因表达综合(GEO)数据库及基因表达谱交互分析(GEPIA)数据库进行生物信息学分析, 选定结直肠癌差异表达预后相关目标基因。收集2016年1月至2022年6月于锦州医科大学附属第三医院行手术治疗的107例结直肠癌患者蜡块组织, 制备组织芯片, 采用免疫组织化学法检测结直肠癌组织中微卫星状态[错配修复(MMR)蛋白MLH1、MSH2、MSH6、PMS2均为阳性表达为错配修复完整(pMMR), 代表微卫星低度不稳定或微卫星稳定;若其中任何一个指标为阴性, 则判定为错配修复缺陷(dMMR), 代表微卫星高度不稳定]和INHBA的表达情况。回顾性分析患者临床病理资料, 分析INHBA与微卫星状态及临床病理特征之间的关系。结果从GEO数据库筛选出结直肠癌3个数据集:GSE110223(13个癌组织、13个癌旁组织)、GSE110224(17个癌组织、17个癌旁组织)、GSE113513(14个癌组织、14个癌旁组织), 筛选出癌组织和癌旁组织间差异表达上调和下调的前50个基因, 经韦恩图分析3个数据集的交集...     

芦丁对人结肠癌SW480细胞凋亡的促进作用及其作用机制

目的:探讨芦丁对人结肠癌(CRC)SW480细胞凋亡的影响,阐明芦丁在CRC治疗中的作用及其可能的分子机制.方法:毒性与基因比较数据库(CTD)和GeneCards数据库预测与芦丁相关的交集mRNA,基因本体(GO)和京都基因与基因组百科全书(KEGG)数据库预测交集mRNA的生物学功能及作用通路,采用String数据...